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The map label for this gene is ptxB [H]

Identifier: 86749051

GI number: 86749051

Start: 2200345

End: 2201304

Strand: Reverse

Name: ptxB [H]

Synonym: RPB_1928

Alternate gene names: 86749051

Gene position: 2201304-2200345 (Counterclockwise)

Preceding gene: 86749052

Following gene: 86749050

Centisome position: 41.29

GC content: 61.56

Gene sequence:

>960_bases
GTGATCCGTTCTGTTCTTCGGACCCTGGCCCTCGGCGCCGCTGTCGGCATGTCTCTGTCGAGCAGCGCGTGGGCCCTCGA
TCCCCGCTTCACCGATGCCGACGGCGACCTGTTGGCCGACGTTCCGACCAACGCGTCGGAGCAGATCGATCCCCCGGTGC
TGGTGTTCGCCTACACGCCCGTGGAAGACCCCGCCGTTTACGCCAAGGTCTGGGAAGGCTTCCTGAAGCATATGGAGCAG
GTCACCGGCAAACGCGTGCAGTTCTTCCCGGTGCAGTCCAACGCCGCCCAGATCGAGGCGATGCGCGCCGGCCGCCTGCA
TGTCGCGGGCTTCAACACCGGGTCCAATCCGATCGCGGTGAACTGCGCCGGCTTCCGCCCGTTCGCGATGATGGCGTCGA
ACAAGGACGAGTTCGGCTACAAGATGGAGATCATCACCTATCCGGGGTCCGGCATCGAGAAGATCGAGGACATCAAGGGT
AAGAAGATGGCCTTCACCTCGGAGACTTCGAACTCCGGCTACAAGGCGCCCTCGGCCCTGCTGCGCGATCAGTTCAAGAT
GGAAGCGCAGAAGGACTACGAGCCGGTGTTCTCCGGCAAGCACGACAATTCGATTCTCGGCGTCGCCAACAAGGATTATC
CAGCCGCCGCGATCGCCTCCTCGGTCAAGGACCGGATGATCGCGCGCAACGTAGTGAAGGCCGATCAGTTGAAGGTGATC
TACGCCTCGGCGCCGTTTCCGACCACCGGCTACGGGATGGTCTACAATCTGAAGCCGGAACTGCAGGCCAAGATCAAGCA
GGCGTTCTTCTCCTACCCGTGGGAAGGCACCGAACTCCTGAAGGAGTTCGAGAAGTCCGATCCGCCGCAGCAGAAGTTCA
TGGAGATCTCGTTCAAGGACAAGTGGCAGGTGGTGCGTCAGATCGATCAGGCGATGGGCGTCAGCTACGCCTGCAAGTAA

Upstream 100 bases:

>100_bases
TTGCACCTCCGGGCCGATCCTTCGTTGTCCTCTCGTCGACCGCATCGTCGCGGTCCGTCTGGACCGACATTTCATTCCCC
TCGACCCTCAGGAGAAATTC

Downstream 100 bases:

>100_bases
CACCCCCCTTGCCGAGCGGCGCCGTCCGGATTGAAATCCGGGCGGCGCGACGCATGTCGGGCGCCGGCCAATGCGATTCG
GGGAAATCACCGTGCTGAGA

Product: phosphonate-binding periplasmic protein

Products: 3-phospho-serine [Cytoplasm]; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MIRSVLRTLALGAAVGMSLSSSAWALDPRFTDADGDLLADVPTNASEQIDPPVLVFAYTPVEDPAVYAKVWEGFLKHMEQ
VTGKRVQFFPVQSNAAQIEAMRAGRLHVAGFNTGSNPIAVNCAGFRPFAMMASNKDEFGYKMEIITYPGSGIEKIEDIKG
KKMAFTSETSNSGYKAPSALLRDQFKMEAQKDYEPVFSGKHDNSILGVANKDYPAAAIASSVKDRMIARNVVKADQLKVI
YASAPFPTTGYGMVYNLKPELQAKIKQAFFSYPWEGTELLKEFEKSDPPQQKFMEISFKDKWQVVRQIDQAMGVSYACK

Sequences:

>Translated_319_residues
MIRSVLRTLALGAAVGMSLSSSAWALDPRFTDADGDLLADVPTNASEQIDPPVLVFAYTPVEDPAVYAKVWEGFLKHMEQ
VTGKRVQFFPVQSNAAQIEAMRAGRLHVAGFNTGSNPIAVNCAGFRPFAMMASNKDEFGYKMEIITYPGSGIEKIEDIKG
KKMAFTSETSNSGYKAPSALLRDQFKMEAQKDYEPVFSGKHDNSILGVANKDYPAAAIASSVKDRMIARNVVKADQLKVI
YASAPFPTTGYGMVYNLKPELQAKIKQAFFSYPWEGTELLKEFEKSDPPQQKFMEISFKDKWQVVRQIDQAMGVSYACK
>Mature_319_residues
MIRSVLRTLALGAAVGMSLSSSAWALDPRFTDADGDLLADVPTNASEQIDPPVLVFAYTPVEDPAVYAKVWEGFLKHMEQ
VTGKRVQFFPVQSNAAQIEAMRAGRLHVAGFNTGSNPIAVNCAGFRPFAMMASNKDEFGYKMEIITYPGSGIEKIEDIKG
KKMAFTSETSNSGYKAPSALLRDQFKMEAQKDYEPVFSGKHDNSILGVANKDYPAAAIASSVKDRMIARNVVKADQLKVI
YASAPFPTTGYGMVYNLKPELQAKIKQAFFSYPWEGTELLKEFEKSDPPQQKFMEISFKDKWQVVRQIDQAMGVSYACK

Specific function: Probably forms part of a binding-protein-dependent phosphite transporter. Required for oxidation of phosphite to phosphate [H]

COG id: COG3221

COG function: function code P; ABC-type phosphate/phosphonate transport system, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015168
- InterPro:   IPR005770 [H]

Pfam domain/function: PF09084 NMT1 [H]

EC number: NA

Molecular weight: Translated: 35277; Mature: 35277

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRSVLRTLALGAAVGMSLSSSAWALDPRFTDADGDLLADVPTNASEQIDPPVLVFAYTP
CHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCEEEECCCCCCCCCCCCEEEEEECC
VEDPAVYAKVWEGFLKHMEQVTGKRVQFFPVQSNAAQIEAMRAGRLHVAGFNTGSNPIAV
CCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHCCEEEEEEECCCCCCEEE
NCAGFRPFAMMASNKDEFGYKMEIITYPGSGIEKIEDIKGKKMAFTSETSNSGYKAPSAL
EECCCCCEEEEECCCCCCCEEEEEEECCCCCHHHHHHCCCCEEEEECCCCCCCCCCHHHH
LRDQFKMEAQKDYEPVFSGKHDNSILGVANKDYPAAAIASSVKDRMIARNVVKADQLKVI
HHHHHHHHHHCCCCHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEE
YASAPFPTTGYGMVYNLKPELQAKIKQAFFSYPWEGTELLKEFEKSDPPQQKFMEISFKD
EECCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHEECCCHH
KWQVVRQIDQAMGVSYACK
HHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIRSVLRTLALGAAVGMSLSSSAWALDPRFTDADGDLLADVPTNASEQIDPPVLVFAYTP
CHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCEEEECCCCCCCCCCCCEEEEEECC
VEDPAVYAKVWEGFLKHMEQVTGKRVQFFPVQSNAAQIEAMRAGRLHVAGFNTGSNPIAV
CCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHCCEEEEEEECCCCCCEEE
NCAGFRPFAMMASNKDEFGYKMEIITYPGSGIEKIEDIKGKKMAFTSETSNSGYKAPSAL
EECCCCCEEEEECCCCCCCEEEEEEECCCCCHHHHHHCCCCEEEEECCCCCCCCCCHHHH
LRDQFKMEAQKDYEPVFSGKHDNSILGVANKDYPAAAIASSVKDRMIARNVVKADQLKVI
HHHHHHHHHHCCCCHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEE
YASAPFPTTGYGMVYNLKPELQAKIKQAFFSYPWEGTELLKEFEKSDPPQQKFMEISFKD
EECCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHEECCCHH
KWQVVRQIDQAMGVSYACK
HHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9791102 [H]