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The map label for this gene is braD [H]

Identifier: 86748785

GI number: 86748785

Start: 1890424

End: 1892337

Strand: Reverse

Name: braD [H]

Synonym: RPB_1662

Alternate gene names: 86748785

Gene position: 1892337-1890424 (Counterclockwise)

Preceding gene: 86748786

Following gene: 86748784

Centisome position: 35.49

GC content: 67.87

Gene sequence:

>1914_bases
GTGGAACTGATATTGGTCCAGGCCCTCAGCGGTCTCGCCAGCGCCGCGTCGCTGTTCCTGGTCGCATCCGGGCTCTCGAT
CATCTTCGGCGTCACGCGCATCGTGAACTTCGCGCACGGCGCGTTCTACATGCTCGGCGCCTATGTCGCCTTCACGCTGA
CCGACCGCTTTTCCGGCCCGCTCGGCTTCTGGGGCGGACTGCTCGCCGCCAGCCTGATCGTGGCGGTGCTCGGCGCGCTG
ATGGAGATGGTGCTGCTGCGCCGCATCTATCACGCGCCCGAGCTGTTCCAATTGCTCGCCACCTTCGGCGTGACGCTGAT
GGTACAGGACATCGTGGTGCTGATCTGGGGCGCCGACGATCTGATGGGGCCGCGCGCGCCCGGCTTCGCCGGCGCCTTCA
GCCTGTTCGGACGCCTGGTGCCGACCTACGACATCCTGCTGATCGTGCTCGGTCCGGTCGTACTCGGCGCATTGTGGCTG
TTGTTCCACCGCACCCGCTGGGGCGTGCTGGTGCGCGCCGCGACACAGGACCGCGACATGGTCGCCTGCCTCGGCGTCAA
TCAGAAGTGGCTGTTCACCAGCGTGTTCGCACTCGGCTGCTTCCTCGCCGCGCTCGGCGGCGCGCTGCAGATCCCGCGCA
CCACCGTGACCCACTACATGGACCTGTCGATCATCGTCGAGGTGTTCGTGGTGGTGGTGATCGGCGGGCTCGGCTCGGTC
ACCGGCGCCTTCATCGCCGCGGTTCTGGTGTCGGAATTGAACGCGCTCGGCATTCTGGTGCTGCCGAAGATCTCGCTGAT
CGCCGTGTTCCTGGTGATGGCGGTGGTGCTGGTGGTGCGGCCCTACGGACTGCTCGGCAAGGCCGAACGCGGCGCCCGCG
CCAGCGCCGGGATCTCGTTCCGGCCCTGGAAGCCGCTGTCGCAATGGGAGCGCATCGGCGCCGCGGTCGTCGTCGTCATC
GCGGCCTGCCTGCCCTTGGTCGCCGGCAACTATCTGTTGAGCGTCGGCGCGGAGATCCTGGTGTTCGTGCTGTTCGCCGC
CAGCCTGCACTTCATGATGACAGTGGGCGGGTTGGCATCGTTCGGCCACGCGGCGTTTTTCGGCCTCGGCGCCTATGGCG
CGGCGTTCGCCGTCAAGTTTCTCGGCGCCCCGATGGAGGTCGCGCTGCTCGTCGGCCCGTTGTTCGGCCTGCTCGGCGCG
GTGGTGTCGGGCTGGTTCTGCGTGCGGCTGTCGGGTGTGTATTTCGCCATGCTGACGCTGGCCTTCGCCCAGATCATCTG
GTCGATCGCGTTCCAGTGGGTCGAGGTGACCGGCGGCGACAACGGCATTCTCGGGATCTGGCCCTCGGCCTGGGCGTCGA
CCCCGCAAGGCTTCTTCTGGCTCACGCTCGGCGTGGTCGTCGCGGGCGTCGCACTGCTGCGGATGCTGGTGTTCTCGCCG
TTCGGCTTCGGTCTGCGGGCGACGCGCGACAATACGCTGCGCTCGGAGGCGATCGGCATCGACCGCAAGTCGGTGCAGTG
GGCCGCGCTGATCGTCGCCGGCACCTTCGCCGGCGTCGCCGGCGCGCTGTTCGCCTTCCTCAAGGGCTCGGTGTTTCCGG
AATCGCTGGCGATCCCGATCTCGGTCGACGGATTGGTGATGGTGCTGCTCGGCGGCATCGAAACGGTGTCGGGCGCGGTG
GTCGGCGCGATCGTCTACAAGGCGCTGGCGATCTGGCTGATGAGCCAGACCGACCTCTCCAAGCTCGTGCTCGGCGCGAT
CATCGTGCTGCTGGTGGTGGCTGCGCCCAAGGGCATCGTCGGTTTCATCGAGGATTGGCGGCATCGCCGGCCGCCGGCGC
CGAACGGCGCCGGGCCGAAGCCGGCCGGCAAATCCGACGCGCTCGGGATCGCCAAGATGGAGGCCGCCGAATGA

Upstream 100 bases:

>100_bases
CGCCCGCACGGCTGCTTCCTCTCTTGCGAACGTCCGCCGCTGACATCAGCGGCGGACACCAGACCCAACCCGCTCCTCCT
CCAGCACGAAAGGCGTCGCC

Downstream 100 bases:

>100_bases
CCGCGTTGCTCGAAGTCAAAGGACTGGTGAAGTCCTACGCCGGAGTTCATGCCGTGCGCGACGTCAGCTTCGCCGTGCGC
CCCGGCGAAATCCTGGCGCT

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 637; Mature: 637

Protein sequence:

>637_residues
MELILVQALSGLASAASLFLVASGLSIIFGVTRIVNFAHGAFYMLGAYVAFTLTDRFSGPLGFWGGLLAASLIVAVLGAL
MEMVLLRRIYHAPELFQLLATFGVTLMVQDIVVLIWGADDLMGPRAPGFAGAFSLFGRLVPTYDILLIVLGPVVLGALWL
LFHRTRWGVLVRAATQDRDMVACLGVNQKWLFTSVFALGCFLAALGGALQIPRTTVTHYMDLSIIVEVFVVVVIGGLGSV
TGAFIAAVLVSELNALGILVLPKISLIAVFLVMAVVLVVRPYGLLGKAERGARASAGISFRPWKPLSQWERIGAAVVVVI
AACLPLVAGNYLLSVGAEILVFVLFAASLHFMMTVGGLASFGHAAFFGLGAYGAAFAVKFLGAPMEVALLVGPLFGLLGA
VVSGWFCVRLSGVYFAMLTLAFAQIIWSIAFQWVEVTGGDNGILGIWPSAWASTPQGFFWLTLGVVVAGVALLRMLVFSP
FGFGLRATRDNTLRSEAIGIDRKSVQWAALIVAGTFAGVAGALFAFLKGSVFPESLAIPISVDGLVMVLLGGIETVSGAV
VGAIVYKALAIWLMSQTDLSKLVLGAIIVLLVVAAPKGIVGFIEDWRHRRPPAPNGAGPKPAGKSDALGIAKMEAAE

Sequences:

>Translated_637_residues
MELILVQALSGLASAASLFLVASGLSIIFGVTRIVNFAHGAFYMLGAYVAFTLTDRFSGPLGFWGGLLAASLIVAVLGAL
MEMVLLRRIYHAPELFQLLATFGVTLMVQDIVVLIWGADDLMGPRAPGFAGAFSLFGRLVPTYDILLIVLGPVVLGALWL
LFHRTRWGVLVRAATQDRDMVACLGVNQKWLFTSVFALGCFLAALGGALQIPRTTVTHYMDLSIIVEVFVVVVIGGLGSV
TGAFIAAVLVSELNALGILVLPKISLIAVFLVMAVVLVVRPYGLLGKAERGARASAGISFRPWKPLSQWERIGAAVVVVI
AACLPLVAGNYLLSVGAEILVFVLFAASLHFMMTVGGLASFGHAAFFGLGAYGAAFAVKFLGAPMEVALLVGPLFGLLGA
VVSGWFCVRLSGVYFAMLTLAFAQIIWSIAFQWVEVTGGDNGILGIWPSAWASTPQGFFWLTLGVVVAGVALLRMLVFSP
FGFGLRATRDNTLRSEAIGIDRKSVQWAALIVAGTFAGVAGALFAFLKGSVFPESLAIPISVDGLVMVLLGGIETVSGAV
VGAIVYKALAIWLMSQTDLSKLVLGAIIVLLVVAAPKGIVGFIEDWRHRRPPAPNGAGPKPAGKSDALGIAKMEAAE
>Mature_637_residues
MELILVQALSGLASAASLFLVASGLSIIFGVTRIVNFAHGAFYMLGAYVAFTLTDRFSGPLGFWGGLLAASLIVAVLGAL
MEMVLLRRIYHAPELFQLLATFGVTLMVQDIVVLIWGADDLMGPRAPGFAGAFSLFGRLVPTYDILLIVLGPVVLGALWL
LFHRTRWGVLVRAATQDRDMVACLGVNQKWLFTSVFALGCFLAALGGALQIPRTTVTHYMDLSIIVEVFVVVVIGGLGSV
TGAFIAAVLVSELNALGILVLPKISLIAVFLVMAVVLVVRPYGLLGKAERGARASAGISFRPWKPLSQWERIGAAVVVVI
AACLPLVAGNYLLSVGAEILVFVLFAASLHFMMTVGGLASFGHAAFFGLGAYGAAFAVKFLGAPMEVALLVGPLFGLLGA
VVSGWFCVRLSGVYFAMLTLAFAQIIWSIAFQWVEVTGGDNGILGIWPSAWASTPQGFFWLTLGVVVAGVALLRMLVFSP
FGFGLRATRDNTLRSEAIGIDRKSVQWAALIVAGTFAGVAGALFAFLKGSVFPESLAIPISVDGLVMVLLGGIETVSGAV
VGAIVYKALAIWLMSQTDLSKLVLGAIIVLLVVAAPKGIVGFIEDWRHRRPPAPNGAGPKPAGKSDALGIAKMEAAE

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=296, Percent_Identity=26.6891891891892, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1789865, Length=306, Percent_Identity=25.1633986928105, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 67007; Mature: 67007

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELILVQALSGLASAASLFLVASGLSIIFGVTRIVNFAHGAFYMLGAYVAFTLTDRFSGP
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LGFWGGLLAASLIVAVLGALMEMVLLRRIYHAPELFQLLATFGVTLMVQDIVVLIWGADD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEECCHH
LMGPRAPGFAGAFSLFGRLVPTYDILLIVLGPVVLGALWLLFHRTRWGVLVRAATQDRDM
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCE
VACLGVNQKWLFTSVFALGCFLAALGGALQIPRTTVTHYMDLSIIVEVFVVVVIGGLGSV
EEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHH
TGAFIAAVLVSELNALGILVLPKISLIAVFLVMAVVLVVRPYGLLGKAERGARASAGISF
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
RPWKPLSQWERIGAAVVVVIAACLPLVAGNYLLSVGAEILVFVLFAASLHFMMTVGGLAS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGHAAFFGLGAYGAAFAVKFLGAPMEVALLVGPLFGLLGAVVSGWFCVRLSGVYFAMLTL
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFAQIIWSIAFQWVEVTGGDNGILGIWPSAWASTPQGFFWLTLGVVVAGVALLRMLVFSP
HHHHHHHHHHHHHHEEECCCCCEEECCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
FGFGLRATRDNTLRSEAIGIDRKSVQWAALIVAGTFAGVAGALFAFLKGSVFPESLAIPI
CCCCCEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
SVDGLVMVLLGGIETVSGAVVGAIVYKALAIWLMSQTDLSKLVLGAIIVLLVVAAPKGIV
CHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHH
GFIEDWRHRRPPAPNGAGPKPAGKSDALGIAKMEAAE
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHCCCCC
>Mature Secondary Structure
MELILVQALSGLASAASLFLVASGLSIIFGVTRIVNFAHGAFYMLGAYVAFTLTDRFSGP
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LGFWGGLLAASLIVAVLGALMEMVLLRRIYHAPELFQLLATFGVTLMVQDIVVLIWGADD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEECCHH
LMGPRAPGFAGAFSLFGRLVPTYDILLIVLGPVVLGALWLLFHRTRWGVLVRAATQDRDM
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCE
VACLGVNQKWLFTSVFALGCFLAALGGALQIPRTTVTHYMDLSIIVEVFVVVVIGGLGSV
EEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHH
TGAFIAAVLVSELNALGILVLPKISLIAVFLVMAVVLVVRPYGLLGKAERGARASAGISF
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
RPWKPLSQWERIGAAVVVVIAACLPLVAGNYLLSVGAEILVFVLFAASLHFMMTVGGLAS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGHAAFFGLGAYGAAFAVKFLGAPMEVALLVGPLFGLLGAVVSGWFCVRLSGVYFAMLTL
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFAQIIWSIAFQWVEVTGGDNGILGIWPSAWASTPQGFFWLTLGVVVAGVALLRMLVFSP
HHHHHHHHHHHHHHEEECCCCCEEECCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
FGFGLRATRDNTLRSEAIGIDRKSVQWAALIVAGTFAGVAGALFAFLKGSVFPESLAIPI
CCCCCEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
SVDGLVMVLLGGIETVSGAVVGAIVYKALAIWLMSQTDLSKLVLGAIIVLLVVAAPKGIV
CHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHH
GFIEDWRHRRPPAPNGAGPKPAGKSDALGIAKMEAAE
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]