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The map label for this gene is rnhB

Identifier: 86748206

GI number: 86748206

Start: 1240599

End: 1241453

Strand: Direct

Name: rnhB

Synonym: RPB_1081

Alternate gene names: 86748206

Gene position: 1240599-1241453 (Clockwise)

Preceding gene: 86748205

Following gene: 86748207

Centisome position: 23.27

GC content: 70.76

Gene sequence:

>855_bases
ATGATTCGGAATTCCACGAAGCCTGCCAAGGCCGCGCCCGGCAAGGCGAAGACGACGCCCAAGGCGGCGAAGTCTGCCGC
GACGTCAAGCAAAGCCACCGGAACGGCGCAGGCCGTCAAAGCCTCGCCCGCCAAGGGACTGGGCAAGGGCGTGATCGCCG
TCGCGCCGCCGAGCTTTCGGCGCGAACGGGCGCTGATCAAGCGCGGCATCTGGCCGGTCGCCGGCTGCGACGAGGCGGGT
CGCGGGCCGCTCGCCGGCCCGGTGGTCGCGGCTGCGGTGGTGCTCGATCCCAAGCGGGTGCCCAAGGGGCTCGACGACTC
CAAGCGGCTCTCAGCCGAGAAGCGCGAAGAGCTGTTCGAGGAAATCTGCGCCAGCGCGCAGGTCTCGGTGGCGTGCGCCT
CGCCGGAGCGGATCAACCGCGACAACATCCTGCGTGCCTCGCTGTGGGCATTGACCCGCGCGGTGCATGCGCTGCCGGAC
CTGCCGCAGCACGTCTTCGTCGACGGCCGCGATCGGCTCGCGACGCGCTGCGAAAGCGAGGCGGTGATCGGCGGCGACGG
GCTGATCGCGTCGATCGCCGCGGCCTCGATCATCGCCAAGGTCACGCGCGACCGGCTGATGTGCCGGCTGGCGCAGGACT
GCCCCGGCTACGGCTTCGAGCAGCACAAGGGCTACGGCGTGCCGGAGCATCTCGCGGCGCTGGCACGGCTCGGCCCGTCC
GTGCATCACCGCCGTTTCTTCGCGCCAGTGGCCGCCGCCTGGCAGAAGATCGAGGGGGCGCCGGCCGAACCGACAGGTCT
CACCGGTGATCTGTTCGGCACACCGGCTGATTCCGGTCACGCAGCCACGCTGTAG

Upstream 100 bases:

>100_bases
GGGAGGGGGCAAGTTCCGGCGGCTTCGAGGTCGCTTGAATAGCGGGCATGGCGCTGAATTTCCGGTTGCGCGTGCCGGTG
CCTGCCCGCAGCATCGCCAC

Downstream 100 bases:

>100_bases
GCGGGGCCAAGCCGGTTGCCTGGTGCGCCGGTGGCCTCTATCACGGGCGAACCGAACTCCGCTGGCCCCGCATGCGCTTC
ACCTCCCTGATCATCGAATT

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MIRNSTKPAKAAPGKAKTTPKAAKSAATSSKATGTAQAVKASPAKGLGKGVIAVAPPSFRRERALIKRGIWPVAGCDEAG
RGPLAGPVVAAAVVLDPKRVPKGLDDSKRLSAEKREELFEEICASAQVSVACASPERINRDNILRASLWALTRAVHALPD
LPQHVFVDGRDRLATRCESEAVIGGDGLIASIAAASIIAKVTRDRLMCRLAQDCPGYGFEQHKGYGVPEHLAALARLGPS
VHHRRFFAPVAAAWQKIEGAPAEPTGLTGDLFGTPADSGHAATL

Sequences:

>Translated_284_residues
MIRNSTKPAKAAPGKAKTTPKAAKSAATSSKATGTAQAVKASPAKGLGKGVIAVAPPSFRRERALIKRGIWPVAGCDEAG
RGPLAGPVVAAAVVLDPKRVPKGLDDSKRLSAEKREELFEEICASAQVSVACASPERINRDNILRASLWALTRAVHALPD
LPQHVFVDGRDRLATRCESEAVIGGDGLIASIAAASIIAKVTRDRLMCRLAQDCPGYGFEQHKGYGVPEHLAALARLGPS
VHHRRFFAPVAAAWQKIEGAPAEPTGLTGDLFGTPADSGHAATL
>Mature_284_residues
MIRNSTKPAKAAPGKAKTTPKAAKSAATSSKATGTAQAVKASPAKGLGKGVIAVAPPSFRRERALIKRGIWPVAGCDEAG
RGPLAGPVVAAAVVLDPKRVPKGLDDSKRLSAEKREELFEEICASAQVSVACASPERINRDNILRASLWALTRAVHALPD
LPQHVFVDGRDRLATRCESEAVIGGDGLIASIAAASIIAKVTRDRLMCRLAQDCPGYGFEQHKGYGVPEHLAALARLGPS
VHHRRFFAPVAAAWQKIEGAPAEPTGLTGDLFGTPADSGHAATL

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=179, Percent_Identity=49.1620111731844, Blast_Score=150, Evalue=7e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_RHOP2 (Q2J169)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_484702.1
- ProteinModelPortal:   Q2J169
- SMR:   Q2J169
- STRING:   Q2J169
- GeneID:   3910167
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_1081
- eggNOG:   COG0164
- HOGENOM:   HBG584843
- OMA:   LFEEICA
- ProtClustDB:   PRK00015
- BioCyc:   RPAL316058:RPB_1081-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 29662; Mature: 29662

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRNSTKPAKAAPGKAKTTPKAAKSAATSSKATGTAQAVKASPAKGLGKGVIAVAPPSFR
CCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCEEEECCCHHH
RERALIKRGIWPVAGCDEAGRGPLAGPVVAAAVVLDPKRVPKGLDDSKRLSAEKREELFE
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHH
EICASAQVSVACASPERINRDNILRASLWALTRAVHALPDLPQHVFVDGRDRLATRCESE
HHHCCCEEEEEECCCHHCCHHHHHHHHHHHHHHHHHHCCCCCHHEEECCHHHHHHHHCCC
AVIGGDGLIASIAAASIIAKVTRDRLMCRLAQDCPGYGFEQHKGYGVPEHLAALARLGPS
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHCCH
VHHRRFFAPVAAAWQKIEGAPAEPTGLTGDLFGTPADSGHAATL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIRNSTKPAKAAPGKAKTTPKAAKSAATSSKATGTAQAVKASPAKGLGKGVIAVAPPSFR
CCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCEEEECCCHHH
RERALIKRGIWPVAGCDEAGRGPLAGPVVAAAVVLDPKRVPKGLDDSKRLSAEKREELFE
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHH
EICASAQVSVACASPERINRDNILRASLWALTRAVHALPDLPQHVFVDGRDRLATRCESE
HHHCCCEEEEEECCCHHCCHHHHHHHHHHHHHHHHHHCCCCCHHEEECCHHHHHHHHCCC
AVIGGDGLIASIAAASIIAKVTRDRLMCRLAQDCPGYGFEQHKGYGVPEHLAALARLGPS
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHCCH
VHHRRFFAPVAAAWQKIEGAPAEPTGLTGDLFGTPADSGHAATL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA