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The map label for this gene is nifB [H]

Identifier: 86748084

GI number: 86748084

Start: 1109005

End: 1110561

Strand: Direct

Name: nifB [H]

Synonym: RPB_0959

Alternate gene names: 86748084

Gene position: 1109005-1110561 (Clockwise)

Preceding gene: 86748083

Following gene: 86748085

Centisome position: 20.8

GC content: 64.68

Gene sequence:

>1557_bases
ATGAGCAAGCTGCTGCAACTCCACGATTTCGGGGCCCCCGGGGCGAAGTCCTTCGAGGCGCTGCGCGCCGGCGCGGCGCA
ATCCGGCTGCAGCACCACGGGCGGCAACGGCAAGTCCGGCTGCGGCTCGGCGGCTGGACAGGGCGATCTGCCGCCGGAGA
TCTGGGAGAAGGTGAAGAACCATCCCTGCTACAGCGAGCAGGCGCATCATCACTTCGCCCGCATGCATGTCGCGGTCGCG
CCCGCATGCAACATCCAGTGCAATTACTGCAATCGCAAATATGACTGCGCCAATGAATCGCGCCCCGGCGTCGTCAGCGA
GAAGCTGACGCCCGAGCAGGCGGCCCGCAAGGTGATCGCGGTGGCCTCGACGATTCCGCAGATGACCGTGCTCGGCATCG
CCGGTCCGGGCGACGCGCTCGCGAACCCTGCCAAGACCTTCAAGACCTTCGAGCTGGTGACGGCCACGGCGCCCGACATC
AAGTTGTGCCTGTCGACCAACGGGCTGATGCTGCCCGACTACGTCGAGCAGATCGCCGCCATGAATGTCGATCACGTCAC
CATCACCATCAACATGATCGATCCGGAGATCGGCGCGCAGATCTATCCGTGGATCTTCTACAACCACCGCCGCTTCACCG
GCGTCGAGGCGTCGAAAATCCTCAGCGAGCGGCAATTGCTCGGGCTCGAGATGCTCACCGCGCGCGGCATCCTGGTGAAG
GTCAATTCGGTGATGATCCCGGGGATCAACGACCGGCACCTGATCGAGGTCAACAAGGCGGTGAAGTCGCGCGGCGCCTT
CCTGCACAACATCATGCCGCTGATCTCCGAGCCGGAGCACGGCACGGTGTTCGGTCTCGAAGGCCGTCGCGGCCCGTCGG
CGCAGGAGCTCAAGGCGCTGCAGGACGATTGCGAAGGCGAGATGAACATGATGCGGCACTGCCGGCAGTGCCGCGCCGAC
GCGGTCGGCCTGCTCGGCGAGGATCGCAGCGCCGAATTCACCACCGACAAGGTGATGGAGATGGAGGTCGAATACGATCT
CGCCGCGCGGCAGGCCTATCAGGCCAAGGTCGAGGCCGAGCGCGACGCCATCGCCCTCGCCAAGCAGCGCGAACTGGCGA
CGCTCGCCGACGAGACCGCCACGATCAAGATCCAGGTCGCGATCGCCACCAAGGGCGGCGGCGTGATCAACGAGCACTTC
GGACATGCTCACGAATTCCAGATCTACGAGGTTTCGACCGCCGGCGCCAAGTTCATCGGTCATCGCCGCGTCGATCTGTA
TTGCGAGGGCGGCTACGCCAGCGAGACCGGCATCGAGCCGATCCTGAAGGCGCTGAACGACTGCACCGCGGTGCTGGTCG
CCAAGATCGGTCTGTGCCCGAAGGATTCTCTGGCCGGCGCCGGGATCGAGGCGGTCGAGGACTACGCCTTCGAATATATC
GAGCAGTCGGTGATCGCTTATTTCAAGGACTACCTCGCGCGCGTCGGGAAGTCGGAAATCCGCCACGTCGCTCGCGGCGA
CGCCGATATTCGCCAGGGCGCGTTCGTCGCGAGTTGA

Upstream 100 bases:

>100_bases
GTTCGCCGCGCCCGCAGTTCATCCGAACATCGCCGGCTCGGTTGCAGCACCCCACCCACGGACAAGCCGCCGATCGTGCG
GCGATCGAACGAGGCCGGAC

Downstream 100 bases:

>100_bases
GGTCGAGCGAGGACATCGGCCATGGCCTACAAGATCGTCGCGTCGCAATGCACCTCCTGCTCGGCCTGCGAGCCGGAGTG
CCCGAACGAGGCGATCAGCG

Product: nitrogenase cofactor biosynthesis protein NifB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MSKLLQLHDFGAPGAKSFEALRAGAAQSGCSTTGGNGKSGCGSAAGQGDLPPEIWEKVKNHPCYSEQAHHHFARMHVAVA
PACNIQCNYCNRKYDCANESRPGVVSEKLTPEQAARKVIAVASTIPQMTVLGIAGPGDALANPAKTFKTFELVTATAPDI
KLCLSTNGLMLPDYVEQIAAMNVDHVTITINMIDPEIGAQIYPWIFYNHRRFTGVEASKILSERQLLGLEMLTARGILVK
VNSVMIPGINDRHLIEVNKAVKSRGAFLHNIMPLISEPEHGTVFGLEGRRGPSAQELKALQDDCEGEMNMMRHCRQCRAD
AVGLLGEDRSAEFTTDKVMEMEVEYDLAARQAYQAKVEAERDAIALAKQRELATLADETATIKIQVAIATKGGGVINEHF
GHAHEFQIYEVSTAGAKFIGHRRVDLYCEGGYASETGIEPILKALNDCTAVLVAKIGLCPKDSLAGAGIEAVEDYAFEYI
EQSVIAYFKDYLARVGKSEIRHVARGDADIRQGAFVAS

Sequences:

>Translated_518_residues
MSKLLQLHDFGAPGAKSFEALRAGAAQSGCSTTGGNGKSGCGSAAGQGDLPPEIWEKVKNHPCYSEQAHHHFARMHVAVA
PACNIQCNYCNRKYDCANESRPGVVSEKLTPEQAARKVIAVASTIPQMTVLGIAGPGDALANPAKTFKTFELVTATAPDI
KLCLSTNGLMLPDYVEQIAAMNVDHVTITINMIDPEIGAQIYPWIFYNHRRFTGVEASKILSERQLLGLEMLTARGILVK
VNSVMIPGINDRHLIEVNKAVKSRGAFLHNIMPLISEPEHGTVFGLEGRRGPSAQELKALQDDCEGEMNMMRHCRQCRAD
AVGLLGEDRSAEFTTDKVMEMEVEYDLAARQAYQAKVEAERDAIALAKQRELATLADETATIKIQVAIATKGGGVINEHF
GHAHEFQIYEVSTAGAKFIGHRRVDLYCEGGYASETGIEPILKALNDCTAVLVAKIGLCPKDSLAGAGIEAVEDYAFEYI
EQSVIAYFKDYLARVGKSEIRHVARGDADIRQGAFVAS
>Mature_517_residues
SKLLQLHDFGAPGAKSFEALRAGAAQSGCSTTGGNGKSGCGSAAGQGDLPPEIWEKVKNHPCYSEQAHHHFARMHVAVAP
ACNIQCNYCNRKYDCANESRPGVVSEKLTPEQAARKVIAVASTIPQMTVLGIAGPGDALANPAKTFKTFELVTATAPDIK
LCLSTNGLMLPDYVEQIAAMNVDHVTITINMIDPEIGAQIYPWIFYNHRRFTGVEASKILSERQLLGLEMLTARGILVKV
NSVMIPGINDRHLIEVNKAVKSRGAFLHNIMPLISEPEHGTVFGLEGRRGPSAQELKALQDDCEGEMNMMRHCRQCRADA
VGLLGEDRSAEFTTDKVMEMEVEYDLAARQAYQAKVEAERDAIALAKQRELATLADETATIKIQVAIATKGGGVINEHFG
HAHEFQIYEVSTAGAKFIGHRRVDLYCEGGYASETGIEPILKALNDCTAVLVAKIGLCPKDSLAGAGIEAVEDYAFEYIE
QSVIAYFKDYLARVGKSEIRHVARGDADIRQGAFVAS

Specific function: Probably involved in the synthesis of the Fe-Mo cofactor [H]

COG id: COG0535

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moaA/nifB/pqqE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003731
- InterPro:   IPR000385
- InterPro:   IPR005980
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02579 Nitro_FeMo-Co; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 56162; Mature: 56031

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLLQLHDFGAPGAKSFEALRAGAAQSGCSTTGGNGKSGCGSAAGQGDLPPEIWEKVKN
CCCCHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHC
HPCYSEQAHHHFARMHVAVAPACNIQCNYCNRKYDCANESRPGVVSEKLTPEQAARKVIA
CCCCCHHHHHHHHHHEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
VASTIPQMTVLGIAGPGDALANPAKTFKTFELVTATAPDIKLCLSTNGLMLPDYVEQIAA
HHHHCCCEEEEEECCCCHHHCCCHHHHHEEEEEEECCCCEEEEECCCCCCCHHHHHHHHH
MNVDHVTITINMIDPEIGAQIYPWIFYNHRRFTGVEASKILSERQLLGLEMLTARGILVK
CCCCEEEEEEEEECCCCCCEEEEEEEECCCEECCCHHHHHHHHHHHHHHHHHHHCCEEEE
VNSVMIPGINDRHLIEVNKAVKSRGAFLHNIMPLISEPEHGTVFGLEGRRGPSAQELKAL
ECCEEECCCCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHH
QDDCEGEMNMMRHCRQCRADAVGLLGEDRSAEFTTDKVMEMEVEYDLAARQAYQAKVEAE
HHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEEEEHHHHHHHHHHHHHHHHH
RDAIALAKQRELATLADETATIKIQVAIATKGGGVINEHFGHAHEFQIYEVSTAGAKFIG
HHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCHHHHCCCCEEEEEEEEECCCHHHHC
HRRVDLYCEGGYASETGIEPILKALNDCTAVLVAKIGLCPKDSLAGAGIEAVEDYAFEYI
CCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
EQSVIAYFKDYLARVGKSEIRHVARGDADIRQGAFVAS
HHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHCCCEECC
>Mature Secondary Structure 
SKLLQLHDFGAPGAKSFEALRAGAAQSGCSTTGGNGKSGCGSAAGQGDLPPEIWEKVKN
CCCHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHC
HPCYSEQAHHHFARMHVAVAPACNIQCNYCNRKYDCANESRPGVVSEKLTPEQAARKVIA
CCCCCHHHHHHHHHHEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
VASTIPQMTVLGIAGPGDALANPAKTFKTFELVTATAPDIKLCLSTNGLMLPDYVEQIAA
HHHHCCCEEEEEECCCCHHHCCCHHHHHEEEEEEECCCCEEEEECCCCCCCHHHHHHHHH
MNVDHVTITINMIDPEIGAQIYPWIFYNHRRFTGVEASKILSERQLLGLEMLTARGILVK
CCCCEEEEEEEEECCCCCCEEEEEEEECCCEECCCHHHHHHHHHHHHHHHHHHHCCEEEE
VNSVMIPGINDRHLIEVNKAVKSRGAFLHNIMPLISEPEHGTVFGLEGRRGPSAQELKAL
ECCEEECCCCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHH
QDDCEGEMNMMRHCRQCRADAVGLLGEDRSAEFTTDKVMEMEVEYDLAARQAYQAKVEAE
HHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEEEEHHHHHHHHHHHHHHHHH
RDAIALAKQRELATLADETATIKIQVAIATKGGGVINEHFGHAHEFQIYEVSTAGAKFIG
HHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCHHHHCCCCEEEEEEEEECCCHHHHC
HRRVDLYCEGGYASETGIEPILKALNDCTAVLVAKIGLCPKDSLAGAGIEAVEDYAFEYI
CCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
EQSVIAYFKDYLARVGKSEIRHVARGDADIRQGAFVAS
HHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1840608 [H]