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The map label for this gene is adh1 [H]

Identifier: 86748071

GI number: 86748071

Start: 1093541

End: 1094728

Strand: Direct

Name: adh1 [H]

Synonym: RPB_0946

Alternate gene names: 86748071

Gene position: 1093541-1094728 (Clockwise)

Preceding gene: 86748070

Following gene: 86748072

Centisome position: 20.51

GC content: 70.2

Gene sequence:

>1188_bases
ATGATCCTGCCTTTCTCGATCGCCCGTCTGCCCCGCATCGAATTCGGCGCAGGCGCCATCGCGAGGTTGCCGAGCCTCGC
AGCCGTCTACGGCAAGCGCCTGCTGCTGGTCACCGGTGCACGCTCGTTCGATCATGCCTCCTATACGGCGCCGTTGCTGG
CCGCGTTGCGCGACCAGAGCTTCAGTATCGCGCGGGTGCCGATCCGCGGCGAGCCGTCGCCCGAGATGGTCGACGACGCC
GTGCGGGAGTGTCGCCGCGCCGAGATCGACGTCGTGATCGGCCTCGGCGGCGGCAGCGCGCTCGATGCCGCCAAGGCGAT
CGCCGGGCTGCTGAAGCCCGGCAATTCGGTGATGGACCATCTCGAAGGCGTCGGCCCCGAACTGCCCTATCGAGGCCCGG
CGACGCCGTTCATCGCGGTGCCGACCACCGCCGGCACCGGCTCCGAGGCGACCAAGAACGCGGTGTTGTCCCGTCACGGC
CCCGATGGCTTCAAGAAATCGTTCCGCGATGAGGCGCTGGTGGCCGAGATCGCGCTGGTCGACCCAGAACTGTTGGCCGG
CTGCCCGCCAGCGCTGATCGCGGCCAACGGCATGGACGCGCTGACCCAGTTGCTCGAATCCTACGTCTCGACCCGCGCCA
ATCCGTTCACCGACGCGCTGGCGCTGTCCGGCCTGCGCGCGGTGCGGGACGGCCTGCTCGCCTGGTACGATGGCGGCGAC
GCCGCGGGCGAGGCGCGCACGCAGATGGCCTACGCCTCGCTGCTGTCCGGCATCTGTCTGGCGCAGACCGGGCTCGGCTC
TGTGCACGGTCTGGCCTCGCCGCTCGGCGCGTTCTTCCCGATCGGCCACGGCGTGGTGTGCGGCACGCTGGTCGCGGATG
CCACTCGCGTCAATATCGCCGCAATGGAAGCGCGTGCGCCGCAACATCCGGCGCTGGCGAAATACGCCGAGATCGGCCGC
CTGCTGTGCGGCGGCTCCGGCAGCGGCGCGGCGCAGGATCGCGACGCTCTGCTGCGCACGCTGCGGCACTGGACGCAGCG
CCTGTCGCTGCCGAGGCTGTCGTCGCTCGGCGTCGCCGCCGCGGACGTCGATCGGATCGTCGCGGCCAGCCGTGGGTCCA
GCATGAAGACCAATCCGGTGGTGCTGACCGACGACGAGATCAGGCTGATCGTGACCTCGCGCTTCTGA

Upstream 100 bases:

>100_bases
GCGGCTGTTGCAATATCTCGCGCCGTAGCGCGCGGGCTGCTAAACCTCACGCCGTGTCGAAGCCCCGCGCGGCCTGCGCG
GGCCTGTGTCCGAGCGCGTG

Downstream 100 bases:

>100_bases
TTTAGATCACGGCGGGTGCCAATTGCCGGGTGCATGGATGGCCTAGGCCTTACACCGCATAAGGTATTTAAGTAGGGTAA
GAGATATAAGATTTCTTGCG

Product: Iron-containing alcohol dehydrogenase

Products: aldehyde; ketone; NADH; H+

Alternate protein names: BDH [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MILPFSIARLPRIEFGAGAIARLPSLAAVYGKRLLLVTGARSFDHASYTAPLLAALRDQSFSIARVPIRGEPSPEMVDDA
VRECRRAEIDVVIGLGGGSALDAAKAIAGLLKPGNSVMDHLEGVGPELPYRGPATPFIAVPTTAGTGSEATKNAVLSRHG
PDGFKKSFRDEALVAEIALVDPELLAGCPPALIAANGMDALTQLLESYVSTRANPFTDALALSGLRAVRDGLLAWYDGGD
AAGEARTQMAYASLLSGICLAQTGLGSVHGLASPLGAFFPIGHGVVCGTLVADATRVNIAAMEARAPQHPALAKYAEIGR
LLCGGSGSGAAQDRDALLRTLRHWTQRLSLPRLSSLGVAAADVDRIVAASRGSSMKTNPVVLTDDEIRLIVTSRF

Sequences:

>Translated_395_residues
MILPFSIARLPRIEFGAGAIARLPSLAAVYGKRLLLVTGARSFDHASYTAPLLAALRDQSFSIARVPIRGEPSPEMVDDA
VRECRRAEIDVVIGLGGGSALDAAKAIAGLLKPGNSVMDHLEGVGPELPYRGPATPFIAVPTTAGTGSEATKNAVLSRHG
PDGFKKSFRDEALVAEIALVDPELLAGCPPALIAANGMDALTQLLESYVSTRANPFTDALALSGLRAVRDGLLAWYDGGD
AAGEARTQMAYASLLSGICLAQTGLGSVHGLASPLGAFFPIGHGVVCGTLVADATRVNIAAMEARAPQHPALAKYAEIGR
LLCGGSGSGAAQDRDALLRTLRHWTQRLSLPRLSSLGVAAADVDRIVAASRGSSMKTNPVVLTDDEIRLIVTSRF
>Mature_395_residues
MILPFSIARLPRIEFGAGAIARLPSLAAVYGKRLLLVTGARSFDHASYTAPLLAALRDQSFSIARVPIRGEPSPEMVDDA
VRECRRAEIDVVIGLGGGSALDAAKAIAGLLKPGNSVMDHLEGVGPELPYRGPATPFIAVPTTAGTGSEATKNAVLSRHG
PDGFKKSFRDEALVAEIALVDPELLAGCPPALIAANGMDALTQLLESYVSTRANPFTDALALSGLRAVRDGLLAWYDGGD
AAGEARTQMAYASLLSGICLAQTGLGSVHGLASPLGAFFPIGHGVVCGTLVADATRVNIAAMEARAPQHPALAKYAEIGR
LLCGGSGSGAAQDRDALLRTLRHWTQRLSLPRLSSLGVAAADVDRIVAASRGSSMKTNPVVLTDDEIRLIVTSRF

Specific function: This enzyme has activity using butanol and ethanol as substrates [H]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=336, Percent_Identity=26.7857142857143, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI48994951, Length=389, Percent_Identity=30.5912596401028, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI87082107, Length=329, Percent_Identity=32.2188449848024, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1787493, Length=352, Percent_Identity=30.6818181818182, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1789163, Length=299, Percent_Identity=31.1036789297659, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI1789386, Length=375, Percent_Identity=25.3333333333333, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17537053, Length=233, Percent_Identity=31.7596566523605, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6321181, Length=345, Percent_Identity=33.3333333333333, Blast_Score=166, Evalue=6e-42,
Organism=Drosophila melanogaster, GI24657991, Length=337, Percent_Identity=28.486646884273, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.1.1

Molecular weight: Translated: 41088; Mature: 41088

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILPFSIARLPRIEFGAGAIARLPSLAAVYGKRLLLVTGARSFDHASYTAPLLAALRDQS
CCCCCCHHHCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHCCC
FSIARVPIRGEPSPEMVDDAVRECRRAEIDVVIGLGGGSALDAAKAIAGLLKPGNSVMDH
CCEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHH
LEGVGPELPYRGPATPFIAVPTTAGTGSEATKNAVLSRHGPDGFKKSFRDEALVAEIALV
HHCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
DPELLAGCPPALIAANGMDALTQLLESYVSTRANPFTDALALSGLRAVRDGLLAWYDGGD
CHHHHHCCCHHHEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEEECCCC
AAGEARTQMAYASLLSGICLAQTGLGSVHGLASPLGAFFPIGHGVVCGTLVADATRVNIA
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEE
AMEARAPQHPALAKYAEIGRLLCGGSGSGAAQDRDALLRTLRHWTQRLSLPRLSSLGVAA
EECCCCCCCCHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCCHH
ADVDRIVAASRGSSMKTNPVVLTDDEIRLIVTSRF
HHHHHHHHHCCCCCCCCCCEEEECCCEEEEEEECC
>Mature Secondary Structure
MILPFSIARLPRIEFGAGAIARLPSLAAVYGKRLLLVTGARSFDHASYTAPLLAALRDQS
CCCCCCHHHCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHCCC
FSIARVPIRGEPSPEMVDDAVRECRRAEIDVVIGLGGGSALDAAKAIAGLLKPGNSVMDH
CCEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHH
LEGVGPELPYRGPATPFIAVPTTAGTGSEATKNAVLSRHGPDGFKKSFRDEALVAEIALV
HHCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
DPELLAGCPPALIAANGMDALTQLLESYVSTRANPFTDALALSGLRAVRDGLLAWYDGGD
CHHHHHCCCHHHEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEEECCCC
AAGEARTQMAYASLLSGICLAQTGLGSVHGLASPLGAFFPIGHGVVCGTLVADATRVNIA
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEE
AMEARAPQHPALAKYAEIGRLLCGGSGSGAAQDRDALLRTLRHWTQRLSLPRLSSLGVAA
EECCCCCCCCHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCCHH
ADVDRIVAASRGSSMKTNPVVLTDDEIRLIVTSRF
HHHHHHHHHCCCCCCCCCCEEEECCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+; NADH [C]

Metal ions: Co2+ Zn2+-depleted [C]

Kcat value (1/min): 150 [C]

Specific activity: 19

Km value (mM): 0.2 {tryptophol}} 0.0074 {NAD+}} 0.0069 {NAD+}} 13 {ethylene} 6 {methanol}} 27 {pentanol}} 0.44 {1-butanol}} 120 {ethanol}} 0.91 {acetaldehyde}} 0.1 {acetaldehyde}} 0.005 {1-octanol}} 0.004 {benzyl} 0.005 {cyclohe

Substrates: alcohol; NAD+

Specific reaction: an alcohol + NAD+ = an aldehyde or ketone + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: 2, 2'-Bipyridyl; 4-Methyl pyrazole acetaldehyde; 8-Amino-6-methoxyquinoline; 8-Hydroxy quinoline-5-sulfonic acid; Acetaldehyde; Dipicolinic acid; EDTA; Ethanol; GSH; Heptafluorbutanol; Imidazole; Iodoacetamide; Iodoacetate; Isobutyramide EDTA; NAD+; NADH;

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2673928 [H]