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The map label for this gene is oprF [H]

Identifier: 86748026

GI number: 86748026

Start: 1036991

End: 1037617

Strand: Reverse

Name: oprF [H]

Synonym: RPB_0900

Alternate gene names: 86748026

Gene position: 1037617-1036991 (Counterclockwise)

Preceding gene: 86748028

Following gene: 86748025

Centisome position: 19.46

GC content: 64.91

Gene sequence:

>627_bases
ATGATGATTCGGCAGATCAAATTCAGCCTGGCGCTGGCCGCCGTCGTTCTGGTGGGCGGCTCTGGTATCAGCATGACCGC
GGCGGAGGCCGGCGAGGTGTCCTCGCAGCAGATTCGCGATCAGCTCAAGACCTCCAAGACCCGCAGCCTGAGCGGAACCC
GCGCCGCAATGAGTCCGGACGATCTGGCGACGATCAAGCGGGTCAGCCGGACCCGGTCGCTGTCGGCCGGCGACCGCGAG
CAGATCGCAGCGATTGCCGCCAAGCGCCCGACGATCGATCTCGAGATCAATTTCGACTACAACTCCGCGACCTTGTCGCC
GCGGGCCGAACCGCAATTGAAGAGCCTCGGCGACGCCCTGACCAGCAGCGACCTGAAGAATTCGATCGTCATGCTGGCCG
GCCATACCGACGCCAAAGGCGGCGACGATTACAATCAGAGCCTGTCGGAACGGCGGGCCGAGGCCGTCAAGCGCTACCTG
ATCGAGCGCTATCGGATCCAGCCGGACAATCTGGTCGCCGTCGGCTACGGCGAGAAGCAGCTCAAGAGCTCGGCCGAGCC
GCTCGCCGCGGAGAACCGCCGCGTCCAGATCGTCAACATGGCCGAGCACGACGAGGCGGCGAAGTAA

Upstream 100 bases:

>100_bases
AGTGACCTATGTCACAGCGAAGCGCAGCACTTGGGCGCCATCCTAATTCCCATGAGGACCGAGCCCGCCGCTGAGCGGCC
TCGGCCAGATGGAGCGAAAC

Downstream 100 bases:

>100_bases
CCTCGTGCCGCGACTGATCGGTCCCGGCGCCGGTGATTGATCGGGCGCTGGGTTGAAGATAGGATCATGGCGATCGTCGA
TGTCGTTCTTGAAGCTATTG

Product: OmpA/MotB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MMIRQIKFSLALAAVVLVGGSGISMTAAEAGEVSSQQIRDQLKTSKTRSLSGTRAAMSPDDLATIKRVSRTRSLSAGDRE
QIAAIAAKRPTIDLEINFDYNSATLSPRAEPQLKSLGDALTSSDLKNSIVMLAGHTDAKGGDDYNQSLSERRAEAVKRYL
IERYRIQPDNLVAVGYGEKQLKSSAEPLAAENRRVQIVNMAEHDEAAK

Sequences:

>Translated_208_residues
MMIRQIKFSLALAAVVLVGGSGISMTAAEAGEVSSQQIRDQLKTSKTRSLSGTRAAMSPDDLATIKRVSRTRSLSAGDRE
QIAAIAAKRPTIDLEINFDYNSATLSPRAEPQLKSLGDALTSSDLKNSIVMLAGHTDAKGGDDYNQSLSERRAEAVKRYL
IERYRIQPDNLVAVGYGEKQLKSSAEPLAAENRRVQIVNMAEHDEAAK
>Mature_208_residues
MMIRQIKFSLALAAVVLVGGSGISMTAAEAGEVSSQQIRDQLKTSKTRSLSGTRAAMSPDDLATIKRVSRTRSLSAGDRE
QIAAIAAKRPTIDLEINFDYNSATLSPRAEPQLKSLGDALTSSDLKNSIVMLAGHTDAKGGDDYNQSLSERRAEAVKRYL
IERYRIQPDNLVAVGYGEKQLKSSAEPLAAENRRVQIVNMAEHDEAAK

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI1787191, Length=94, Percent_Identity=40.4255319148936, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1480 Molecules/Cell In: Growth Phase, Mi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 22534; Mature: 22534

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS01068 OMPA_1 ; PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMIRQIKFSLALAAVVLVGGSGISMTAAEAGEVSSQQIRDQLKTSKTRSLSGTRAAMSPD
CCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCH
DLATIKRVSRTRSLSAGDREQIAAIAAKRPTIDLEINFDYNSATLSPRAEPQLKSLGDAL
HHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHH
TSSDLKNSIVMLAGHTDAKGGDDYNQSLSERRAEAVKRYLIERYRIQPDNLVAVGYGEKQ
HHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHH
LKSSAEPLAAENRRVQIVNMAEHDEAAK
HHCCCCCCCCCCCEEEEEEECCCCCCCC
>Mature Secondary Structure
MMIRQIKFSLALAAVVLVGGSGISMTAAEAGEVSSQQIRDQLKTSKTRSLSGTRAAMSPD
CCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCH
DLATIKRVSRTRSLSAGDREQIAAIAAKRPTIDLEINFDYNSATLSPRAEPQLKSLGDAL
HHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHH
TSSDLKNSIVMLAGHTDAKGGDDYNQSLSERRAEAVKRYLIERYRIQPDNLVAVGYGEKQ
HHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHH
LKSSAEPLAAENRRVQIVNMAEHDEAAK
HHCCCCCCCCCCCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1898935 [H]