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The map label for this gene is metZ [H]

Identifier: 86747917

GI number: 86747917

Start: 886386

End: 887612

Strand: Reverse

Name: metZ [H]

Synonym: RPB_0791

Alternate gene names: 86747917

Gene position: 887612-886386 (Counterclockwise)

Preceding gene: 86747919

Following gene: 86747913

Centisome position: 16.65

GC content: 67.81

Gene sequence:

>1227_bases
ATGTCCGAGACCAATACGCCCAATCCGAAGCCCTCCATCGCCATCCCCGACACCGCGCATTTCCGCCGCGAAACCCGGCT
GGTGCATTCCGGCAGCCTGCGCTCGCAATATGGCGAGACCTCCGAGGCGCTGTTTCTGACCCAGGGCTTCGTCTACGACA
GCGCCGAGCAATGCGAGGCGCGCTTCACCGGCGACGACGCCGGCTTCCAATATTCGCGGTTCTCCAATCCGACGGTGTTC
AGCTTCGAGCAGCGGATGGCGGAATTCGAGGGCGCCGAGGCGGCGCGCGCCACCGCCACCGGCATGGCCGCGGTGACCGC
CGCGATGCTGGCGCCGCTGCGCGCCGGCGATCACGTCGTGGCCTCGAAGGCGATGTTCGGCTCGTGCCGCTACGTGGTCG
AGGATCTGCTGCCGCGCTACGGCATCGAATCGACGCTGGTCGACGGCCTCGATCTCGACCAGTGGCAGAAGGCGCTGCGC
CCGAACACCAGGACCTGCTTCCTCGAAAGCCCGACCAATCCGACGCTCGACGTGCTCGACATCGGCGCGATCGCCGAGAT
CGCGCATGCCGGCGGCGCGCGGCTCGTCGTCGACAACGTGTTCGCGACGCCGATCTGGCAGAGCCCGCTCGCGCTCGGCG
CCGACGTCGTGGTGTACTCCGCCACCAAGCATATCGACGGCCAGGGCCGCTGCCTCGGCGGCGTGGTGCTGTCGACGCAG
GCGTTCATCGAGGAGCACATCCAGATGTATCTGCGCCAGACCGGGCCGTCGCTGTCGCCGTTCAACGCCTGGGTACTGCT
GAAGGGGCTGGAGACGCTGGCGGTCCGGGTTCGCGCCCAGACTGAGACGGCGGCGGCGATTGCCGATGCGCTGGCCGGCC
ATCCGAAGATCCCGCGGCTGGTCTATCCCGGCCGCGCCGACCATCCGCAGGCCGCCACGGTCAAGAAGCAGATGGGCGCC
GGCTCGACGCTGATCGGCTTCGAGGTCGCGGGCGGCAAGGCGGAAGCGTTCCGCTTCCTCAACGCGCTGAAGCTGATCCG
GATCAGCAACAATCTCGGCGACGCCAAGAGCCTGGTCACGCATCCGGCGACGACGACCCATCAGCGGCTGAAGCCGGAAG
CGCGCGCCGAACTCGGCATCAGCGAAGGCTTCATCCGCTATTCGGTCGGCCTCGAGCACAAGGACGACCTGATCGAGGAC
ATCGTCGCGGCGCTGGAGAAGGTGTAG

Upstream 100 bases:

>100_bases
CTAAACAGGCCGGGGACAATGTGATCCCGGCTTGTGGAAATTTTTCCAGGCCGGTTTTTTTGTGCCCGCATCGCCAGGTG
TGGTCACGAATGGAGACCAC

Downstream 100 bases:

>100_bases
CGCCGCGCGAGTTTCGTGCCCCGGATGCTGCGCAGCGCGCCGCCATCCTGGACGCGGCGACGGGTACCCACGTCATCCTG
AGGTGCGCGCTCTTGCGCGC

Product: O-succinylhomoserine sulfhydrylase

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MSETNTPNPKPSIAIPDTAHFRRETRLVHSGSLRSQYGETSEALFLTQGFVYDSAEQCEARFTGDDAGFQYSRFSNPTVF
SFEQRMAEFEGAEAARATATGMAAVTAAMLAPLRAGDHVVASKAMFGSCRYVVEDLLPRYGIESTLVDGLDLDQWQKALR
PNTRTCFLESPTNPTLDVLDIGAIAEIAHAGGARLVVDNVFATPIWQSPLALGADVVVYSATKHIDGQGRCLGGVVLSTQ
AFIEEHIQMYLRQTGPSLSPFNAWVLLKGLETLAVRVRAQTETAAAIADALAGHPKIPRLVYPGRADHPQAATVKKQMGA
GSTLIGFEVAGGKAEAFRFLNALKLIRISNNLGDAKSLVTHPATTTHQRLKPEARAELGISEGFIRYSVGLEHKDDLIED
IVAALEKV

Sequences:

>Translated_408_residues
MSETNTPNPKPSIAIPDTAHFRRETRLVHSGSLRSQYGETSEALFLTQGFVYDSAEQCEARFTGDDAGFQYSRFSNPTVF
SFEQRMAEFEGAEAARATATGMAAVTAAMLAPLRAGDHVVASKAMFGSCRYVVEDLLPRYGIESTLVDGLDLDQWQKALR
PNTRTCFLESPTNPTLDVLDIGAIAEIAHAGGARLVVDNVFATPIWQSPLALGADVVVYSATKHIDGQGRCLGGVVLSTQ
AFIEEHIQMYLRQTGPSLSPFNAWVLLKGLETLAVRVRAQTETAAAIADALAGHPKIPRLVYPGRADHPQAATVKKQMGA
GSTLIGFEVAGGKAEAFRFLNALKLIRISNNLGDAKSLVTHPATTTHQRLKPEARAELGISEGFIRYSVGLEHKDDLIED
IVAALEKV
>Mature_407_residues
SETNTPNPKPSIAIPDTAHFRRETRLVHSGSLRSQYGETSEALFLTQGFVYDSAEQCEARFTGDDAGFQYSRFSNPTVFS
FEQRMAEFEGAEAARATATGMAAVTAAMLAPLRAGDHVVASKAMFGSCRYVVEDLLPRYGIESTLVDGLDLDQWQKALRP
NTRTCFLESPTNPTLDVLDIGAIAEIAHAGGARLVVDNVFATPIWQSPLALGADVVVYSATKHIDGQGRCLGGVVLSTQA
FIEEHIQMYLRQTGPSLSPFNAWVLLKGLETLAVRVRAQTETAAAIADALAGHPKIPRLVYPGRADHPQAATVKKQMGAG
STLIGFEVAGGKAEAFRFLNALKLIRISNNLGDAKSLVTHPATTTHQRLKPEARAELGISEGFIRYSVGLEHKDDLIEDI
VAALEKV

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=328, Percent_Identity=34.7560975609756, Blast_Score=206, Evalue=3e-53,
Organism=Homo sapiens, GI299473759, Length=328, Percent_Identity=32.0121951219512, Blast_Score=176, Evalue=4e-44,
Organism=Homo sapiens, GI25453487, Length=327, Percent_Identity=28.4403669724771, Blast_Score=146, Evalue=3e-35,
Organism=Escherichia coli, GI1790375, Length=345, Percent_Identity=35.6521739130435, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1789383, Length=394, Percent_Identity=27.1573604060914, Blast_Score=137, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17560056, Length=332, Percent_Identity=37.0481927710843, Blast_Score=231, Evalue=4e-61,
Organism=Caenorhabditis elegans, GI17538055, Length=332, Percent_Identity=36.7469879518072, Blast_Score=228, Evalue=6e-60,
Organism=Caenorhabditis elegans, GI71980708, Length=354, Percent_Identity=33.6158192090396, Blast_Score=202, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6319307, Length=390, Percent_Identity=33.8461538461538, Blast_Score=207, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6323333, Length=410, Percent_Identity=30.2439024390244, Blast_Score=172, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6321254, Length=408, Percent_Identity=26.2254901960784, Blast_Score=135, Evalue=8e-33,
Organism=Saccharomyces cerevisiae, GI6321170, Length=307, Percent_Identity=25.4071661237785, Blast_Score=114, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6321904, Length=349, Percent_Identity=28.3667621776504, Blast_Score=110, Evalue=4e-25,
Organism=Drosophila melanogaster, GI19922554, Length=389, Percent_Identity=32.9048843187661, Blast_Score=214, Evalue=9e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 44050; Mature: 43919

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETNTPNPKPSIAIPDTAHFRRETRLVHSGSLRSQYGETSEALFLTQGFVYDSAEQCEA
CCCCCCCCCCCCEECCCHHHHHHHHHHHHCCCHHHHCCCCCCEEEEEECCCCCCHHHHCC
RFTGDDAGFQYSRFSNPTVFSFEQRMAEFEGAEAARATATGMAAVTAAMLAPLRAGDHVV
EECCCCCCEEECCCCCCCEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHH
ASKAMFGSCRYVVEDLLPRYGIESTLVDGLDLDQWQKALRPNTRTCFLESPTNPTLDVLD
HHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHCCCCCEEEEECCCCCCCHHEE
IGAIAEIAHAGGARLVVDNVFATPIWQSPLALGADVVVYSATKHIDGQGRCLGGVVLSTQ
HHHHHHHHHCCCCEEEEECHHCCCCCCCCHHCCCCEEEEECHHCCCCCCCEEHHHHHHHH
AFIEEHIQMYLRQTGPSLSPFNAWVLLKGLETLAVRVRAQTETAAAIADALAGHPKIPRL
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHEEEECCHHHHHHHHHHHCCCCCCCEE
VYPGRADHPQAATVKKQMGAGSTLIGFEVAGGKAEAFRFLNALKLIRISNNLGDAKSLVT
ECCCCCCCCCHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCHHHHHC
HPATTTHQRLKPEARAELGISEGFIRYSVGLEHKDDLIEDIVAALEKV
CCCCCHHHHCCCHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SETNTPNPKPSIAIPDTAHFRRETRLVHSGSLRSQYGETSEALFLTQGFVYDSAEQCEA
CCCCCCCCCCCEECCCHHHHHHHHHHHHCCCHHHHCCCCCCEEEEEECCCCCCHHHHCC
RFTGDDAGFQYSRFSNPTVFSFEQRMAEFEGAEAARATATGMAAVTAAMLAPLRAGDHVV
EECCCCCCEEECCCCCCCEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHH
ASKAMFGSCRYVVEDLLPRYGIESTLVDGLDLDQWQKALRPNTRTCFLESPTNPTLDVLD
HHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHCCCCCEEEEECCCCCCCHHEE
IGAIAEIAHAGGARLVVDNVFATPIWQSPLALGADVVVYSATKHIDGQGRCLGGVVLSTQ
HHHHHHHHHCCCCEEEEECHHCCCCCCCCHHCCCCEEEEECHHCCCCCCCEEHHHHHHHH
AFIEEHIQMYLRQTGPSLSPFNAWVLLKGLETLAVRVRAQTETAAAIADALAGHPKIPRL
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHEEEECCHHHHHHHHHHHCCCCCCCEE
VYPGRADHPQAATVKKQMGAGSTLIGFEVAGGKAEAFRFLNALKLIRISNNLGDAKSLVT
ECCCCCCCCCHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCHHHHHC
HPATTTHQRLKPEARAELGISEGFIRYSVGLEHKDDLIEDIVAALEKV
CCCCCHHHHCCCHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]