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The map label for this gene is rpoN2 [H]

Identifier: 86747781

GI number: 86747781

Start: 739032

End: 740672

Strand: Reverse

Name: rpoN2 [H]

Synonym: RPB_0655

Alternate gene names: 86747781

Gene position: 740672-739032 (Counterclockwise)

Preceding gene: 86747782

Following gene: 86747780

Centisome position: 13.89

GC content: 66.54

Gene sequence:

>1641_bases
ATGGCACTAACTCAACGCCTCGAGTTCCGACAGTCACAGTCGCTGGTGATGACGCCGCAGCTGATGCAGGCGATCAAGCT
GCTGCAACTGTCGAATCTGGACCTCGCGACCTTCGTCGAGGACGAACTCGAGAAAAACCCCCTGCTGGACCGGGCCAGTG
ACAACGCCGAACCGCCGGTCGCCGGCGAGGCAGTGATGGAGCGCGCCGAGGCCAGCGGCGACGATTTCGGCGGGAGCGAG
AGCAGCGGAGACGGCTCTGACTTCTCCGACGGCGTCGGCAGCGACTCGTTCGAGCCGGGCGCCGAGGATTGGATGCACCG
CGACCTCGGCAGCCGCAGCGAGATCGAGCAGACGCTCGATACCGGCATGGAAAACGTGTTTCCGGAAGAGCCGGCCGAGG
CCGCCGCCCGCGCCGCGCAGGACGCGGCTCCAGCCTCCTACACCGAATGGGGCGGCGGCGCCTCCAGCGACGAGGGCTAC
AATCTCGAGGCCTTCGTCGCCGCCGAAACCTCCCTGGCCGATCGCCTCGCCGAGCAGCTTGCGGTGGCGCTCACCGCGCC
GTCGCAGCGCATGATCGGCCAATATCTGATCGACCTCGTGGATGACGCCGGATATCTGCCGCCCGACCTCGGTGACGCCG
CGGAGCGTCTCGGCACCACCCAGGCCGAAGTCGAAGCCGTCGTCGCCGTTCTGCAGACCTTCGATCCGCCGGGGATCTGC
GCGCGCTCGCTGGCCGAATGCCTGGCGATCCAGTTGCGCGAACTCGACCGGTTCGACCCGGCGATGCAGGCTTTGGTCGA
GAATCTGGATCTCCTCGCCAAGCGCGACATCGCCAGCCTCCGCAAGCTCTGCGGCGTCGACGACGAGGATCTCGCCGATA
TGATCGGCGAAATCCGCCATCTCGACCCGAAGCCGGGTCTGAAATTCGCATCGTCGCGGGTGCAGACCGTGGTGCCGGAC
GTGTTCGTCCGCCCCGGCCCGGACGGCGGCTGGCTGGTCGAACTCAACAGCGACACGCTGCCGAAGGTGCTGGTCAACCA
GTCCTATTACTCCGAACTGTCGAAGACGATCCGCAAGGACGGCGACAAGTCGTACTTCTCCGACTGCCTGCAGAACGCCA
CCTGGCTGGTGCGCGCGCTCGACCAGCGTGCCCGCACCATCCTGAAAGTGGCGACCGAGATCGTGCGCCAGCAGGACGGC
TTCTTCACCCACGGCGTCGCGCATCTGCGGCCGCTGAATCTGAAGGCGGTGGCCGACGCGATCCAGATGCACGAATCCAC
GGTATCGCGCGTGACCGCCAACAAATACATGGCGACCAATCGGGGCACGTTCGAACTCAAGTATTTCTTTACCGCTTCGA
TCGCTTCCGCCGACGGCGGCGAGGCGCATTCGGCCGAAGCCGTGCGCCATCACATCCGGCAGTTGATCGACGGCGAAGAG
CCGACCGCCATCCTGTCGGACGACACCATCGTCGAACGGCTGCGCGAAGCCGGCATCGAGATTGCGCGCCGCACCGTCGC
GAAGTATCGCGAGGCGATGCGGATCCCGTCGTCGGTGCAGCGGCGGCGCGACAAGCAGAGCATGCTCGGCACGGCCCTGG
CGGCGCCCGCCGATCGGTCCCGCGACACCGCTCCGGCTTGA

Upstream 100 bases:

>100_bases
GGTGGCCCCCTACTCCGAATTTCCAATTCGTCAAGGCGTGTACATCAGCCGCGGAAAGGCGTAAGCAAGAAACGGACCAA
CTTGGATCGTTCCTGCCGCC

Downstream 100 bases:

>100_bases
TTACGCCGCTGCTCGGACTACTGTCATTACCCCCAAGGACGAAACGAGGCATCCCATGACTTTTCGGGTCTCCGGCAAAA
GCATCAGCGTCGGCGAAGCC

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MALTQRLEFRQSQSLVMTPQLMQAIKLLQLSNLDLATFVEDELEKNPLLDRASDNAEPPVAGEAVMERAEASGDDFGGSE
SSGDGSDFSDGVGSDSFEPGAEDWMHRDLGSRSEIEQTLDTGMENVFPEEPAEAAARAAQDAAPASYTEWGGGASSDEGY
NLEAFVAAETSLADRLAEQLAVALTAPSQRMIGQYLIDLVDDAGYLPPDLGDAAERLGTTQAEVEAVVAVLQTFDPPGIC
ARSLAECLAIQLRELDRFDPAMQALVENLDLLAKRDIASLRKLCGVDDEDLADMIGEIRHLDPKPGLKFASSRVQTVVPD
VFVRPGPDGGWLVELNSDTLPKVLVNQSYYSELSKTIRKDGDKSYFSDCLQNATWLVRALDQRARTILKVATEIVRQQDG
FFTHGVAHLRPLNLKAVADAIQMHESTVSRVTANKYMATNRGTFELKYFFTASIASADGGEAHSAEAVRHHIRQLIDGEE
PTAILSDDTIVERLREAGIEIARRTVAKYREAMRIPSSVQRRRDKQSMLGTALAAPADRSRDTAPA

Sequences:

>Translated_546_residues
MALTQRLEFRQSQSLVMTPQLMQAIKLLQLSNLDLATFVEDELEKNPLLDRASDNAEPPVAGEAVMERAEASGDDFGGSE
SSGDGSDFSDGVGSDSFEPGAEDWMHRDLGSRSEIEQTLDTGMENVFPEEPAEAAARAAQDAAPASYTEWGGGASSDEGY
NLEAFVAAETSLADRLAEQLAVALTAPSQRMIGQYLIDLVDDAGYLPPDLGDAAERLGTTQAEVEAVVAVLQTFDPPGIC
ARSLAECLAIQLRELDRFDPAMQALVENLDLLAKRDIASLRKLCGVDDEDLADMIGEIRHLDPKPGLKFASSRVQTVVPD
VFVRPGPDGGWLVELNSDTLPKVLVNQSYYSELSKTIRKDGDKSYFSDCLQNATWLVRALDQRARTILKVATEIVRQQDG
FFTHGVAHLRPLNLKAVADAIQMHESTVSRVTANKYMATNRGTFELKYFFTASIASADGGEAHSAEAVRHHIRQLIDGEE
PTAILSDDTIVERLREAGIEIARRTVAKYREAMRIPSSVQRRRDKQSMLGTALAAPADRSRDTAPA
>Mature_545_residues
ALTQRLEFRQSQSLVMTPQLMQAIKLLQLSNLDLATFVEDELEKNPLLDRASDNAEPPVAGEAVMERAEASGDDFGGSES
SGDGSDFSDGVGSDSFEPGAEDWMHRDLGSRSEIEQTLDTGMENVFPEEPAEAAARAAQDAAPASYTEWGGGASSDEGYN
LEAFVAAETSLADRLAEQLAVALTAPSQRMIGQYLIDLVDDAGYLPPDLGDAAERLGTTQAEVEAVVAVLQTFDPPGICA
RSLAECLAIQLRELDRFDPAMQALVENLDLLAKRDIASLRKLCGVDDEDLADMIGEIRHLDPKPGLKFASSRVQTVVPDV
FVRPGPDGGWLVELNSDTLPKVLVNQSYYSELSKTIRKDGDKSYFSDCLQNATWLVRALDQRARTILKVATEIVRQQDGF
FTHGVAHLRPLNLKAVADAIQMHESTVSRVTANKYMATNRGTFELKYFFTASIASADGGEAHSAEAVRHHIRQLIDGEEP
TAILSDDTIVERLREAGIEIARRTVAKYREAMRIPSSVQRRRDKQSMLGTALAAPADRSRDTAPA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the nitrogen fixation genes [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=532, Percent_Identity=35.7142857142857, Blast_Score=305, Evalue=3e-84,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 59499; Mature: 59368

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALTQRLEFRQSQSLVMTPQLMQAIKLLQLSNLDLATFVEDELEKNPLLDRASDNAEPPV
CCCHHHHHHHHCCCEEECHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCC
AGEAVMERAEASGDDFGGSESSGDGSDFSDGVGSDSFEPGAEDWMHRDLGSRSEIEQTLD
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHH
TGMENVFPEEPAEAAARAAQDAAPASYTEWGGGASSDEGYNLEAFVAAETSLADRLAEQL
HHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHH
AVALTAPSQRMIGQYLIDLVDDAGYLPPDLGDAAERLGTTQAEVEAVVAVLQTFDPPGIC
HHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHCCCCCHH
ARSLAECLAIQLRELDRFDPAMQALVENLDLLAKRDIASLRKLCGVDDEDLADMIGEIRH
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
LDPKPGLKFASSRVQTVVPDVFVRPGPDGGWLVELNSDTLPKVLVNQSYYSELSKTIRKD
CCCCCCHHHHHHHHHHHCCHHEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCC
GDKSYFSDCLQNATWLVRALDQRARTILKVATEIVRQQDGFFTHGVAHLRPLNLKAVADA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCHHHHCCCCHHHHHHH
IQMHESTVSRVTANKYMATNRGTFELKYFFTASIASADGGEAHSAEAVRHHIRQLIDGEE
HHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEHHCCCCCCCHHHHHHHHHHHHHHCCCC
PTAILSDDTIVERLREAGIEIARRTVAKYREAMRIPSSVQRRRDKQSMLGTALAAPADRS
CCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCC
RDTAPA
CCCCCC
>Mature Secondary Structure 
ALTQRLEFRQSQSLVMTPQLMQAIKLLQLSNLDLATFVEDELEKNPLLDRASDNAEPPV
CCHHHHHHHHCCCEEECHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCC
AGEAVMERAEASGDDFGGSESSGDGSDFSDGVGSDSFEPGAEDWMHRDLGSRSEIEQTLD
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHH
TGMENVFPEEPAEAAARAAQDAAPASYTEWGGGASSDEGYNLEAFVAAETSLADRLAEQL
HHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHH
AVALTAPSQRMIGQYLIDLVDDAGYLPPDLGDAAERLGTTQAEVEAVVAVLQTFDPPGIC
HHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHCCCCCHH
ARSLAECLAIQLRELDRFDPAMQALVENLDLLAKRDIASLRKLCGVDDEDLADMIGEIRH
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
LDPKPGLKFASSRVQTVVPDVFVRPGPDGGWLVELNSDTLPKVLVNQSYYSELSKTIRKD
CCCCCCHHHHHHHHHHHCCHHEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCC
GDKSYFSDCLQNATWLVRALDQRARTILKVATEIVRQQDGFFTHGVAHLRPLNLKAVADA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCHHHHCCCCHHHHHHH
IQMHESTVSRVTANKYMATNRGTFELKYFFTASIASADGGEAHSAEAVRHHIRQLIDGEE
HHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEHHCCCCCCCHHHHHHHHHHHHHHCCCC
PTAILSDDTIVERLREAGIEIARRTVAKYREAMRIPSSVQRRRDKQSMLGTALAAPADRS
CCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCC
RDTAPA
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1991712; 12597275 [H]