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The map label for this gene is aroA

Identifier: 86747769

GI number: 86747769

Start: 727983

End: 729320

Strand: Reverse

Name: aroA

Synonym: RPB_0643

Alternate gene names: 86747769

Gene position: 729320-727983 (Counterclockwise)

Preceding gene: 86747779

Following gene: 86747767

Centisome position: 13.68

GC content: 72.12

Gene sequence:

>1338_bases
TTGAGCCATTCCGACCATACCGCGCCGCTCGAAGCGCGCCTCAGCGCGGCGCTGAGCGGCACCGCCCGCGTCCCCGGCGA
CAAGTCGATCTCCCACCGGGCGCTGATTCTCGGCGCGCTCGCGGTCGGCGAGACCCGGATTTCCGGCCTGCTCGAGGGCG
AGGACGTGCTCAACACCGCCCGGGCGATGCGGGCGCTGGGGGCGCAGGTCGAGCGCACCGGCGATTGCGCCTGGAGCGTC
CACGGCGTCGGCGTCGCGGGGTTCGCGCCGCCCGCGGCGCCGCTCGATTTCGGCAATTCCGGCACCGGCTGCCGGCTGGC
GATGGGCGCGGTGGCGGGCTCGCCGATCATCGCCACCTTCGACGGCGACGCCTCGCTGCGCTCGCGGCCGATGCGCCGGA
TCGTCGATCCGCTGGAGCAGATGGGCGCCCGGGTGACGCAGAGCGCCGACGGCGGCCGGCTGCCGCTGACGCTGCAGGGC
GCGCGCGACCCGCTGCCGATCACCTACCGCACCCCCGTACCTTCGGCGCAGATCAAATCCGCGGTGCTGCTCGCCGGCCT
GTCGGCGCCGGGCGTCACCACCGTGATCGAGGCCGAGGCCAGCCGCGACCATACCGAGCTGATGCTGCAGCATTTCGGCG
CCACGGTCGTGACCGAGCCGGAAGGCCCCCATGGCCGGAAGATTTCGCTGACCGGGCAGCCCGAGCTGCGCGGCGCGCCG
GTGGTGGTGCCGGCGGACCCGTCCTCGGCGGCGTTCCCGATGGTCGCGGCGCTGATCGTGCCGGGCTCCGACGTGGTGCT
GACCGAGGTGATGACCAACCCGCTGCGCACCGGCCTGATCACCACGCTGCGCGAGATGGGCGGCCTGATCGAGGAGAGCG
AAACCCGCGGCGACGCCGGCGAGCCGATGGCGCGCTTCCGCATCCGCGGCTCGCAATTGCGCGGCGTCGAAGTGCCGCCG
GAGCGCGCTCCGTCGATGATCGACGAATATCTGGTGCTGGCGGTCGCGGCCGCCTTCGCCGAGGGCACCACGATCATGCG
CGGCCTGCACGAGCTGCGGGTCAAGGAAAGCGACCGGCTGGAAGCGACCGCGGCGATGCTGCGGGTCAATGGCGTGACGG
TCGAGATCTCGGGCGACGATCTGATCGTCGAGGGCAAAGGCCACGTCCCGGGCGGCGGGCTGGTCGCCACCCACATGGAT
CACCGCATCGCGATGTCGGCGCTGGTGATGGGGCTGGCCGCCGACAAGCCGGTCAGGGTCGACGACACCGCCTTCATCGC
CACCAGCTTCCCGGATTTCGTCCCGATGATGCAAAGGCTCGGCGCCGAATTCGGCTGA

Upstream 100 bases:

>100_bases
TGCCACGCCGCTGTCAATAGCGGTTTATGCGGGAGCGACCGCTGCGCGCTGCGCGCGGCCGCCTTCGATCAGGCGCCCGG
CGCGCAGGGGACCCGAAATC

Downstream 100 bases:

>100_bases
CCCACAGCCTCTACGCCGGCTCGACCGGCACGCGGCCGACCAGCCGGTCGGTCTCCTGCTGCGCGGCGGTGAGGGACGCC
GACAGCCGGGCACGGAGCTC

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MSHSDHTAPLEARLSAALSGTARVPGDKSISHRALILGALAVGETRISGLLEGEDVLNTARAMRALGAQVERTGDCAWSV
HGVGVAGFAPPAAPLDFGNSGTGCRLAMGAVAGSPIIATFDGDASLRSRPMRRIVDPLEQMGARVTQSADGGRLPLTLQG
ARDPLPITYRTPVPSAQIKSAVLLAGLSAPGVTTVIEAEASRDHTELMLQHFGATVVTEPEGPHGRKISLTGQPELRGAP
VVVPADPSSAAFPMVAALIVPGSDVVLTEVMTNPLRTGLITTLREMGGLIEESETRGDAGEPMARFRIRGSQLRGVEVPP
ERAPSMIDEYLVLAVAAAFAEGTTIMRGLHELRVKESDRLEATAAMLRVNGVTVEISGDDLIVEGKGHVPGGGLVATHMD
HRIAMSALVMGLAADKPVRVDDTAFIATSFPDFVPMMQRLGAEFG

Sequences:

>Translated_445_residues
MSHSDHTAPLEARLSAALSGTARVPGDKSISHRALILGALAVGETRISGLLEGEDVLNTARAMRALGAQVERTGDCAWSV
HGVGVAGFAPPAAPLDFGNSGTGCRLAMGAVAGSPIIATFDGDASLRSRPMRRIVDPLEQMGARVTQSADGGRLPLTLQG
ARDPLPITYRTPVPSAQIKSAVLLAGLSAPGVTTVIEAEASRDHTELMLQHFGATVVTEPEGPHGRKISLTGQPELRGAP
VVVPADPSSAAFPMVAALIVPGSDVVLTEVMTNPLRTGLITTLREMGGLIEESETRGDAGEPMARFRIRGSQLRGVEVPP
ERAPSMIDEYLVLAVAAAFAEGTTIMRGLHELRVKESDRLEATAAMLRVNGVTVEISGDDLIVEGKGHVPGGGLVATHMD
HRIAMSALVMGLAADKPVRVDDTAFIATSFPDFVPMMQRLGAEFG
>Mature_444_residues
SHSDHTAPLEARLSAALSGTARVPGDKSISHRALILGALAVGETRISGLLEGEDVLNTARAMRALGAQVERTGDCAWSVH
GVGVAGFAPPAAPLDFGNSGTGCRLAMGAVAGSPIIATFDGDASLRSRPMRRIVDPLEQMGARVTQSADGGRLPLTLQGA
RDPLPITYRTPVPSAQIKSAVLLAGLSAPGVTTVIEAEASRDHTELMLQHFGATVVTEPEGPHGRKISLTGQPELRGAPV
VVPADPSSAAFPMVAALIVPGSDVVLTEVMTNPLRTGLITTLREMGGLIEESETRGDAGEPMARFRIRGSQLRGVEVPPE
RAPSMIDEYLVLAVAAAFAEGTTIMRGLHELRVKESDRLEATAAMLRVNGVTVEISGDDLIVEGKGHVPGGGLVATHMDH
RIAMSALVMGLAADKPVRVDDTAFIATSFPDFVPMMQRLGAEFG

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family

Homologues:

Organism=Escherichia coli, GI1787137, Length=435, Percent_Identity=31.4942528735632, Blast_Score=129, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6320332, Length=474, Percent_Identity=27.8481012658228, Blast_Score=105, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROA_RHOP2 (Q2J2F6)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_484265.1
- HSSP:   P22487
- ProteinModelPortal:   Q2J2F6
- SMR:   Q2J2F6
- STRING:   Q2J2F6
- GeneID:   3908336
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_0643
- eggNOG:   COG0128
- HOGENOM:   HBG646626
- OMA:   LSRNHTE
- ProtClustDB:   PRK02427
- BioCyc:   RPAL316058:RPB_0643-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00210
- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.10
- PIRSF:   PIRSF000505
- TIGRFAMs:   TIGR01356

Pfam domain/function: PF00275 EPSP_synthase; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =2.5.1.19

Molecular weight: Translated: 46445; Mature: 46314

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS00104 EPSP_SYNTHASE_1; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHSDHTAPLEARLSAALSGTARVPGDKSISHRALILGALAVGETRISGLLEGEDVLNTA
CCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHEEEHHHHCHHHHHHCCCCHHHHHHH
RAMRALGAQVERTGDCAWSVHGVGVAGFAPPAAPLDFGNSGTGCRLAMGAVAGSPIIATF
HHHHHHHHHHHCCCCEEEEEECCEEECCCCCCCCCCCCCCCCCCEEEEHHCCCCCEEEEE
DGDASLRSRPMRRIVDPLEQMGARVTQSADGGRLPLTLQGARDPLPITYRTPVPSAQIKS
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCEEEECCCCCHHHHH
AVLLAGLSAPGVTTVIEAEASRDHTELMLQHFGATVVTEPEGPHGRKISLTGQPELRGAP
HHHEEECCCCCCEEEEECCCCCCHHHHHHHHCCCEEEECCCCCCCCEEEECCCCCCCCCC
VVVPADPSSAAFPMVAALIVPGSDVVLTEVMTNPLRTGLITTLREMGGLIEESETRGDAG
EEEECCCCCCHHHHHHHEEECCCCEEHHHHHHCHHHHHHHHHHHHHCCHHHCCCCCCCCC
EPMARFRIRGSQLRGVEVPPERAPSMIDEYLVLAVAAAFAEGTTIMRGLHELRVKESDRL
CCHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHH
EATAAMLRVNGVTVEISGDDLIVEGKGHVPGGGLVATHMDHRIAMSALVMGLAADKPVRV
HHHHHEEEECCEEEEECCCEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCEE
DDTAFIATSFPDFVPMMQRLGAEFG
CCCEEEECCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SHSDHTAPLEARLSAALSGTARVPGDKSISHRALILGALAVGETRISGLLEGEDVLNTA
CCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHEEEHHHHCHHHHHHCCCCHHHHHHH
RAMRALGAQVERTGDCAWSVHGVGVAGFAPPAAPLDFGNSGTGCRLAMGAVAGSPIIATF
HHHHHHHHHHHCCCCEEEEEECCEEECCCCCCCCCCCCCCCCCCEEEEHHCCCCCEEEEE
DGDASLRSRPMRRIVDPLEQMGARVTQSADGGRLPLTLQGARDPLPITYRTPVPSAQIKS
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCEEEECCCCCHHHHH
AVLLAGLSAPGVTTVIEAEASRDHTELMLQHFGATVVTEPEGPHGRKISLTGQPELRGAP
HHHEEECCCCCCEEEEECCCCCCHHHHHHHHCCCEEEECCCCCCCCEEEECCCCCCCCCC
VVVPADPSSAAFPMVAALIVPGSDVVLTEVMTNPLRTGLITTLREMGGLIEESETRGDAG
EEEECCCCCCHHHHHHHEEECCCCEEHHHHHHCHHHHHHHHHHHHHCCHHHCCCCCCCCC
EPMARFRIRGSQLRGVEVPPERAPSMIDEYLVLAVAAAFAEGTTIMRGLHELRVKESDRL
CCHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHH
EATAAMLRVNGVTVEISGDDLIVEGKGHVPGGGLVATHMDHRIAMSALVMGLAADKPVRV
HHHHHEEEECCEEEEECCCEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCEE
DDTAFIATSFPDFVPMMQRLGAEFG
CCCEEEECCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA