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The map label for this gene is mutM

Identifier: 86747745

GI number: 86747745

Start: 698153

End: 699034

Strand: Reverse

Name: mutM

Synonym: RPB_0619

Alternate gene names: 86747745

Gene position: 699034-698153 (Counterclockwise)

Preceding gene: 86747757

Following gene: 86747743

Centisome position: 13.11

GC content: 68.37

Gene sequence:

>882_bases
ATGCCTGAACTGCCCGAAGTCGAAACCGTCCGCCTCGGGCTGCAGCCGGCCATGGAGGGATTCCGGATCGACCGCGCGAT
GGCGAACCGCTGCGATCTGCGGTTTCCGTTTCAACCCGACTTCGCCGCGCGGCTGACCGGGCAGACCATCACCGGGCTGG
GGCGCCGCGCCAAATATCTGTTGGCCGATCTGTCGAGCGGCGACGTGCTGCTGATGCATCTCGGCATGTCGGGCTCGTTC
CGGGTGGTGAACGGCGCCGGCGACGCGACGCCGGGCGAGTTTCACCATCCGCGCAGCGAGGACCGCACCCACGATCACGT
CGTGTTCGAGATGTCGAGCGGCGCGCGGGTGATCTTCAACGACCCGCGGCGATTCGGCTTCATGAAGATCTTTGCGCGCG
CCGCGATCGACGACGAGCCGCATCTCAAGGGGCTCGGGCCCGAGCCATTGGGCAACGCCTTCGACGCCGCGATGCTGGCG
CGGGCCTGCGCCGGCAAGCAGACCAGCCTGAAGGCGGCGCTGCTCGACCAGCGCGTCGTCGCCGGGCTCGGCAACATTTA
TGTCTGCGAGGCGCTGTGGCGCGCGCATCTGTCGCCGAAGAGGAAGGCGTCGACGCTGGCCGACCGCAAAGGCGCGCCGA
CCGATCGCGCCGTGCGGCTGGTGGATGCAATCCGCGCCGTGCTCGGCGACGCCATCAAGGCCGGCGGCTCGTCACTGCGC
GACCACCGCCAGACCTCCGGCGAACTCGGTTACTTCCAGCATTCCTTCGCGGTGTACGACCGCGAAGGCGAGCGCTGCCG
CACGCCGGGATGCAATGGGACGGTGAAGCGGCTGGTGCAGAACGGGCGGTCGACGTTCTGGTGTTCGGGTTGCCAGACGT
AG

Upstream 100 bases:

>100_bases
GCGCGATGCCGCCCGGAACACGAAATGCCGCTCCGCGGCGGACCATATAGCGCGCCGGGGCGGCAGGCGCTATCACCTGA
ATGCCTTATGGGACCCCAGC

Downstream 100 bases:

>100_bases
GTGCGGCGAAGGCGCCGGCGCCGTGCTCGGCTCGCCCTCACCCCAACCCTCTCCCGCAAGCGGGAGAGGGGGCTCGCCGT
GCGCGGGGTAAGGCCCTGCA

Product: formamidopyrimidine-DNA glycosylase

Products: NA

Alternate protein names: Fapy-DNA glycosylase; DNA-(apurinic or apyrimidinic site) lyase mutM; AP lyase mutM

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MPELPEVETVRLGLQPAMEGFRIDRAMANRCDLRFPFQPDFAARLTGQTITGLGRRAKYLLADLSSGDVLLMHLGMSGSF
RVVNGAGDATPGEFHHPRSEDRTHDHVVFEMSSGARVIFNDPRRFGFMKIFARAAIDDEPHLKGLGPEPLGNAFDAAMLA
RACAGKQTSLKAALLDQRVVAGLGNIYVCEALWRAHLSPKRKASTLADRKGAPTDRAVRLVDAIRAVLGDAIKAGGSSLR
DHRQTSGELGYFQHSFAVYDREGERCRTPGCNGTVKRLVQNGRSTFWCSGCQT

Sequences:

>Translated_293_residues
MPELPEVETVRLGLQPAMEGFRIDRAMANRCDLRFPFQPDFAARLTGQTITGLGRRAKYLLADLSSGDVLLMHLGMSGSF
RVVNGAGDATPGEFHHPRSEDRTHDHVVFEMSSGARVIFNDPRRFGFMKIFARAAIDDEPHLKGLGPEPLGNAFDAAMLA
RACAGKQTSLKAALLDQRVVAGLGNIYVCEALWRAHLSPKRKASTLADRKGAPTDRAVRLVDAIRAVLGDAIKAGGSSLR
DHRQTSGELGYFQHSFAVYDREGERCRTPGCNGTVKRLVQNGRSTFWCSGCQT
>Mature_292_residues
PELPEVETVRLGLQPAMEGFRIDRAMANRCDLRFPFQPDFAARLTGQTITGLGRRAKYLLADLSSGDVLLMHLGMSGSFR
VVNGAGDATPGEFHHPRSEDRTHDHVVFEMSSGARVIFNDPRRFGFMKIFARAAIDDEPHLKGLGPEPLGNAFDAAMLAR
ACAGKQTSLKAALLDQRVVAGLGNIYVCEALWRAHLSPKRKASTLADRKGAPTDRAVRLVDAIRAVLGDAIKAGGSSLRD
HRQTSGELGYFQHSFAVYDREGERCRTPGCNGTVKRLVQNGRSTFWCSGCQT

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyr

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger

Homologues:

Organism=Escherichia coli, GI1790066, Length=292, Percent_Identity=38.6986301369863, Blast_Score=186, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FPG_RHOP2 (Q2J2I0)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_484241.1
- HSSP:   P42371
- ProteinModelPortal:   Q2J2I0
- SMR:   Q2J2I0
- STRING:   Q2J2I0
- GeneID:   3908312
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_0619
- eggNOG:   COG0266
- HOGENOM:   HBG690070
- OMA:   RSTFYCA
- ProtClustDB:   PRK01103
- BioCyc:   RPAL316058:RPB_0619-MONOMER
- HAMAP:   MF_00103
- InterPro:   IPR015886
- InterPro:   IPR015887
- InterPro:   IPR000191
- InterPro:   IPR012319
- InterPro:   IPR020629
- InterPro:   IPR010979
- InterPro:   IPR000214
- InterPro:   IPR010663
- SMART:   SM00898
- TIGRFAMs:   TIGR00577

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH; PF06827 zf-FPG_IleRS; SSF81624 Form_DNAglyc_cat; SSF46946 Ribosomal_H2TH

EC number: =3.2.2.23; =4.2.99.18

Molecular weight: Translated: 32026; Mature: 31895

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: PS51068 FPG_CAT; PS01242 ZF_FPG_1; PS51066 ZF_FPG_2

Important sites: ACT_SITE 2-2 ACT_SITE 3-3 ACT_SITE 58-58 ACT_SITE 283-283 BINDING 104-104 BINDING 123-123 BINDING 166-166

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELPEVETVRLGLQPAMEGFRIDRAMANRCDLRFPFQPDFAARLTGQTITGLGRRAKYL
CCCCCCCHHEEECCHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHCCCHHHHHHHHHHHH
LADLSSGDVLLMHLGMSGSFRVVNGAGDATPGEFHHPRSEDRTHDHVVFEMSSGARVIFN
HEECCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEC
DPRRFGFMKIFARAAIDDEPHLKGLGPEPLGNAFDAAMLARACAGKQTSLKAALLDQRVV
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
AGLGNIYVCEALWRAHLSPKRKASTLADRKGAPTDRAVRLVDAIRAVLGDAIKAGGSSLR
HHCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHH
DHRQTSGELGYFQHSFAVYDREGERCRTPGCNGTVKRLVQNGRSTFWCSGCQT
HHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHCCCCEEEECCCCC
>Mature Secondary Structure 
PELPEVETVRLGLQPAMEGFRIDRAMANRCDLRFPFQPDFAARLTGQTITGLGRRAKYL
CCCCCCHHEEECCHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHCCCHHHHHHHHHHHH
LADLSSGDVLLMHLGMSGSFRVVNGAGDATPGEFHHPRSEDRTHDHVVFEMSSGARVIFN
HEECCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEC
DPRRFGFMKIFARAAIDDEPHLKGLGPEPLGNAFDAAMLARACAGKQTSLKAALLDQRVV
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
AGLGNIYVCEALWRAHLSPKRKASTLADRKGAPTDRAVRLVDAIRAVLGDAIKAGGSSLR
HHCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHH
DHRQTSGELGYFQHSFAVYDREGERCRTPGCNGTVKRLVQNGRSTFWCSGCQT
HHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA