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The map label for this gene is gyaR [H]

Identifier: 86747699

GI number: 86747699

Start: 645749

End: 646735

Strand: Direct

Name: gyaR [H]

Synonym: RPB_0573

Alternate gene names: 86747699

Gene position: 645749-646735 (Clockwise)

Preceding gene: 86747698

Following gene: 86747700

Centisome position: 12.11

GC content: 68.49

Gene sequence:

>987_bases
ATGGCGGGGGAAACAGGCTCGGCGGACGCCATCGATCTGCTGATCTACGGGCCGACCAAGCCGGTGGTGAACGAGGGCTT
TCCCGCGTGCTACGCCCTGCACCGATGCGAGCAGCCGGCCGATCTCGACCGCCTCGGCGATGACATCCGCGCCCGCATCC
GCGGCGTCGCCGTCACCGGCCTGGTGCCGACCCAGGCGGCGATGCTGGCGCGCTACCCGAAGCTCGAGATCGTCTCGTCG
TTCGGCGTCGGCTACGACCACATCGATTCCTCTTGGGCCGCGCAGCACAATGTCGTCGTCACCAACACGCCCGACGTGCT
GACCGAGGAAGTCGCCGACACCGCGCTCGGCCTGCTGATCGCCACATTGCGCGAATTCGTCCAGGCCGACGCCCATGTCC
GCTCCGGGCTGTGGGCGAATCAGGCCTATCCGCTCAGTAAAGGCTCGCTGCGCGACCGCATGGTCGGCATCGTCGGCATG
GGGCGGATCGGGCAGGCGATCGCACGGCGGCTCGACGCCTCGCTGGTGCCGGTGGTGTATCACTCGCGCAAGCCGGCGCC
GGGGGTGGACTACAAGCACTACCCCGATCTGATCGCGATGGCGAAAGCCGTCGACACGCTGATCGTGATCATCCCGGGCG
GCGCCTCCACATTGAGACTGATCAACGCCGAGGTGCTGGCCGCGCTCGGCCCGCGCGGCGTGCTGATCAACGTCGCGCGC
GGATCGGTGGTCGACGAGCCGGCGCTGATCGCGGCGCTGCAATCCGGCACGATCCTCGCCGCCGGCCTCGACGTGTTCGC
CGACGAGCCGAACGTGCCGGACGAATTGCGCGCGCTGCCGAACGTGATCCTGCTGCCGCATATCGGCTCGGCCTCGGTGG
TGACGCGCAGCGCGATGGACCAGCTCGTGGTCGACAACATCAAGGCGTGGTTCGACGGCAAGCCGCCGTTGACGCCGATT
CCCGAAACCCCGGTGAAGGGCCGCTGA

Upstream 100 bases:

>100_bases
TGCTCTCGGCGGCGGGCATTGTCATCTATGCGGCGCTGGCGCTGCTGTCGCATCTGCTGCTGCGGCGCTGGCACGAAAGT
GCGCTCGGAAAGGAACAATG

Downstream 100 bases:

>100_bases
GATGAGACGCTGCGTCACCGCCGCCATGTTGTTGCTGCTGAGCGCCGCGCCGGCGCCGGCGCAGGACGCCGCGGCGCTGA
CCAAGGCGATGGTCGGGCAG

Product: D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MAGETGSADAIDLLIYGPTKPVVNEGFPACYALHRCEQPADLDRLGDDIRARIRGVAVTGLVPTQAAMLARYPKLEIVSS
FGVGYDHIDSSWAAQHNVVVTNTPDVLTEEVADTALGLLIATLREFVQADAHVRSGLWANQAYPLSKGSLRDRMVGIVGM
GRIGQAIARRLDASLVPVVYHSRKPAPGVDYKHYPDLIAMAKAVDTLIVIIPGGASTLRLINAEVLAALGPRGVLINVAR
GSVVDEPALIAALQSGTILAAGLDVFADEPNVPDELRALPNVILLPHIGSASVVTRSAMDQLVVDNIKAWFDGKPPLTPI
PETPVKGR

Sequences:

>Translated_328_residues
MAGETGSADAIDLLIYGPTKPVVNEGFPACYALHRCEQPADLDRLGDDIRARIRGVAVTGLVPTQAAMLARYPKLEIVSS
FGVGYDHIDSSWAAQHNVVVTNTPDVLTEEVADTALGLLIATLREFVQADAHVRSGLWANQAYPLSKGSLRDRMVGIVGM
GRIGQAIARRLDASLVPVVYHSRKPAPGVDYKHYPDLIAMAKAVDTLIVIIPGGASTLRLINAEVLAALGPRGVLINVAR
GSVVDEPALIAALQSGTILAAGLDVFADEPNVPDELRALPNVILLPHIGSASVVTRSAMDQLVVDNIKAWFDGKPPLTPI
PETPVKGR
>Mature_327_residues
AGETGSADAIDLLIYGPTKPVVNEGFPACYALHRCEQPADLDRLGDDIRARIRGVAVTGLVPTQAAMLARYPKLEIVSSF
GVGYDHIDSSWAAQHNVVVTNTPDVLTEEVADTALGLLIATLREFVQADAHVRSGLWANQAYPLSKGSLRDRMVGIVGMG
RIGQAIARRLDASLVPVVYHSRKPAPGVDYKHYPDLIAMAKAVDTLIVIIPGGASTLRLINAEVLAALGPRGVLINVARG
SVVDEPALIAALQSGTILAAGLDVFADEPNVPDELRALPNVILLPHIGSASVVTRSAMDQLVVDNIKAWFDGKPPLTPIP
ETPVKGR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GyaR subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=250, Percent_Identity=36.8, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI23308577, Length=227, Percent_Identity=30.3964757709251, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI61743967, Length=259, Percent_Identity=28.5714285714286, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI4557497, Length=259, Percent_Identity=28.5714285714286, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI145580578, Length=259, Percent_Identity=27.027027027027, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI4557499, Length=259, Percent_Identity=27.027027027027, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI145580575, Length=269, Percent_Identity=27.1375464684015, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI87082289, Length=256, Percent_Identity=39.453125, Blast_Score=168, Evalue=4e-43,
Organism=Escherichia coli, GI1789279, Length=259, Percent_Identity=30.1158301158301, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87081824, Length=234, Percent_Identity=30.3418803418803, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787645, Length=269, Percent_Identity=21.9330855018587, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17532191, Length=246, Percent_Identity=30.4878048780488, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI25147481, Length=287, Percent_Identity=25.0871080139373, Blast_Score=81, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6324055, Length=279, Percent_Identity=35.84229390681, Blast_Score=149, Evalue=7e-37,
Organism=Saccharomyces cerevisiae, GI6322116, Length=283, Percent_Identity=24.7349823321555, Blast_Score=95, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6320925, Length=277, Percent_Identity=25.2707581227437, Blast_Score=90, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6325144, Length=182, Percent_Identity=33.5164835164835, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321253, Length=247, Percent_Identity=26.7206477732794, Blast_Score=79, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6324964, Length=275, Percent_Identity=21.8181818181818, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI28571528, Length=252, Percent_Identity=39.6825396825397, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24585514, Length=298, Percent_Identity=32.8859060402685, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI28574282, Length=298, Percent_Identity=32.8859060402685, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI28574284, Length=298, Percent_Identity=32.8859060402685, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI45552429, Length=322, Percent_Identity=32.2981366459627, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI45551003, Length=298, Percent_Identity=32.8859060402685, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24585516, Length=265, Percent_Identity=32.8301886792453, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI28574286, Length=255, Percent_Identity=33.7254901960784, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI62472511, Length=262, Percent_Identity=30.1526717557252, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24646446, Length=262, Percent_Identity=30.1526717557252, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24646448, Length=262, Percent_Identity=30.1526717557252, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24646452, Length=262, Percent_Identity=30.1526717557252, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24646450, Length=262, Percent_Identity=30.1526717557252, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19921140, Length=227, Percent_Identity=29.0748898678414, Blast_Score=95, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.26 [H]

Molecular weight: Translated: 34784; Mature: 34652

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGETGSADAIDLLIYGPTKPVVNEGFPACYALHRCEQPADLDRLGDDIRARIRGVAVTG
CCCCCCCCCEEEEEEECCCCCHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEEEC
LVPTQAAMLARYPKLEIVSSFGVGYDHIDSSWAAQHNVVVTNTPDVLTEEVADTALGLLI
CCHHHHHHHHHCCCHHHHHHCCCCHHHCCCCHHCCCCEEEECCCHHHHHHHHHHHHHHHH
ATLREFVQADAHVRSGLWANQAYPLSKGSLRDRMVGIVGMGRIGQAIARRLDASLVPVVY
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEE
HSRKPAPGVDYKHYPDLIAMAKAVDTLIVIIPGGASTLRLINAEVLAALGPRGVLINVAR
ECCCCCCCCCCCCCHHHHHHHHHHCEEEEEECCCCCEEHHHHHHHHHHCCCCCEEEEECC
GSVVDEPALIAALQSGTILAAGLDVFADEPNVPDELRALPNVILLPHIGSASVVTRSAMD
CCCCCCCHHHHHHCCCEEEEECCHHCCCCCCCCHHHHHCCCEEEEECCCCHHHHHHHHHH
QLVVDNIKAWFDGKPPLTPIPETPVKGR
HHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AGETGSADAIDLLIYGPTKPVVNEGFPACYALHRCEQPADLDRLGDDIRARIRGVAVTG
CCCCCCCCEEEEEEECCCCCHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEEEC
LVPTQAAMLARYPKLEIVSSFGVGYDHIDSSWAAQHNVVVTNTPDVLTEEVADTALGLLI
CCHHHHHHHHHCCCHHHHHHCCCCHHHCCCCHHCCCCEEEECCCHHHHHHHHHHHHHHHH
ATLREFVQADAHVRSGLWANQAYPLSKGSLRDRMVGIVGMGRIGQAIARRLDASLVPVVY
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEE
HSRKPAPGVDYKHYPDLIAMAKAVDTLIVIIPGGASTLRLINAEVLAALGPRGVLINVAR
ECCCCCCCCCCCCCHHHHHHHHHHCEEEEEECCCCCEEHHHHHHHHHHCCCCCEEEEECC
GSVVDEPALIAALQSGTILAAGLDVFADEPNVPDELRALPNVILLPHIGSASVVTRSAMD
CCCCCCCHHHHHHCCCEEEEECCHHCCCCCCCCHHHHHCCCEEEEECCCCHHHHHHHHHH
QLVVDNIKAWFDGKPPLTPIPETPVKGR
HHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA