BacMap

Definition	Rhodopseudomonas palustris HaA2, complete genome.
Accession	NC_007778
Length	5,331,656

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The map label for this gene is paaG [C]

Identifier: 86747681

GI number: 86747681

Start: 625777

End: 626565

Strand: Direct

Name: paaG [C]

Synonym: RPB_0555

Alternate gene names: 86747681

Gene position: 625777-626565 (Clockwise)

Preceding gene: 86747680

Following gene: 86747682

Centisome position: 11.74

GC content: 70.47

Gene sequence:

>789_bases
ATGACCGACATCGTCCTGCAGCAGCTCGACCGCGGCCTGCTCACCATCACCATGAACCGGCCCGACCGGCGCAATGCGCT
CAATCAGGACATGATCCGCGGGCTGGTCGAGGCGGCGCATCGGGCCAAGGACGATCCGCAGGTCCGCGCGGTGCTGCTGA
AGGGCGGCGGCGGCACGTTCTGCGTCGGCGGCGACGTCAAGTCGATGGCGGCGGCCGGCGATCCGCCGCCGCTGGAGCAG
AAGGTCGCCAATCTGCGCCGCGGCATGGAGGTCTCGCGGCTCCTGCATCAGATGCCGAAGCCCGTGGTGGCGCAGATCGA
CGGCGCGGCGGCCGGCGCCGGGCTGTCGATGGCGCTGGCCTGCGACCTGCGCGTCGCGGGCGCCTCCGCCAAGATCACCA
CCGCCTTCGCCAAGGTCGGCCTGTCGGGCGATTTCGGCGGCACCTATTTCCTCACGCATCTGATCGGCGCCGCCAGGGCG
CGCGACCTGTATCTGACCTCCCCGGTGTTGACCGCGCCCGAGGCGCTGGCGCTCGGCATCGTCAGCCGCGTCGTGCCGGA
CGCCGAGGTCGAGAGCGCCACGCGGGATCTGGCGCTGTCGCTGGCGCAGGGGCCGAGCGTGACGCTCGGCTACATCAAGA
CCAACATCACCAACGCCGAGACTCTCTCGCTCGAAGCCTGCTTCGACGCCGAGGCGCTGCATCATTCGCGCTGCGCCGAG
ACCGCCGACCACAAGGAAGCCGCCGCGGCCTTTGTGGAGAAGCGCGCGGCCGTGTTTCAGGGGCGATGA

Upstream 100 bases:

>100_bases
TCGCAATGACGGAAACAGTTGAAGTTCGAAACGGCGCTGCGCGCCTCCTCACACGATGAGGAGTCCGGCCGGCAACCGCC
CAACAACAAGGAATCACCCC

Downstream 100 bases:

>100_bases
CGATGACGCCGTTTCTCAGGCTGCGCCAGATCTGCCTCGTCGCCGACGCCATCGAACCCGCGGCGTCGCAGCTCGCGGCG
ATGATGGGGCTGACCGTCTG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MTDIVLQQLDRGLLTITMNRPDRRNALNQDMIRGLVEAAHRAKDDPQVRAVLLKGGGGTFCVGGDVKSMAAAGDPPPLEQ
KVANLRRGMEVSRLLHQMPKPVVAQIDGAAAGAGLSMALACDLRVAGASAKITTAFAKVGLSGDFGGTYFLTHLIGAARA
RDLYLTSPVLTAPEALALGIVSRVVPDAEVESATRDLALSLAQGPSVTLGYIKTNITNAETLSLEACFDAEALHHSRCAE
TADHKEAAAAFVEKRAAVFQGR

Sequences:

>Translated_262_residues
MTDIVLQQLDRGLLTITMNRPDRRNALNQDMIRGLVEAAHRAKDDPQVRAVLLKGGGGTFCVGGDVKSMAAAGDPPPLEQ
KVANLRRGMEVSRLLHQMPKPVVAQIDGAAAGAGLSMALACDLRVAGASAKITTAFAKVGLSGDFGGTYFLTHLIGAARA
RDLYLTSPVLTAPEALALGIVSRVVPDAEVESATRDLALSLAQGPSVTLGYIKTNITNAETLSLEACFDAEALHHSRCAE
TADHKEAAAAFVEKRAAVFQGR
>Mature_261_residues
TDIVLQQLDRGLLTITMNRPDRRNALNQDMIRGLVEAAHRAKDDPQVRAVLLKGGGGTFCVGGDVKSMAAAGDPPPLEQK
VANLRRGMEVSRLLHQMPKPVVAQIDGAAAGAGLSMALACDLRVAGASAKITTAFAKVGLSGDFGGTYFLTHLIGAARAR
DLYLTSPVLTAPEALALGIVSRVVPDAEVESATRDLALSLAQGPSVTLGYIKTNITNAETLSLEACFDAEALHHSRCAET
ADHKEAAAAFVEKRAAVFQGR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI70995211, Length=188, Percent_Identity=32.4468085106383, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI194097323, Length=247, Percent_Identity=31.9838056680162, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI4502327, Length=268, Percent_Identity=31.7164179104478, Blast_Score=98, Evalue=7e-21,
Organism=Homo sapiens, GI213417737, Length=245, Percent_Identity=26.530612244898, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI157694516, Length=245, Percent_Identity=26.530612244898, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI31542718, Length=261, Percent_Identity=26.4367816091954, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI45643119, Length=221, Percent_Identity=28.5067873303167, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI260275230, Length=221, Percent_Identity=28.5067873303167, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI260274832, Length=221, Percent_Identity=28.5067873303167, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI68989263, Length=188, Percent_Identity=32.4468085106383, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI37594469, Length=185, Percent_Identity=30.8108108108108, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI37594471, Length=185, Percent_Identity=30.8108108108108, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI25453481, Length=186, Percent_Identity=30.6451612903226, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI51538573, Length=186, Percent_Identity=30.6451612903226, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI4757966, Length=186, Percent_Identity=30.6451612903226, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI51538581, Length=186, Percent_Identity=30.6451612903226, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI4757968, Length=186, Percent_Identity=30.1075268817204, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI49355787, Length=186, Percent_Identity=30.1075268817204, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI221307494, Length=185, Percent_Identity=29.7297297297297, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI20127408, Length=202, Percent_Identity=26.7326732673267, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI221136756, Length=184, Percent_Identity=29.3478260869565, Blast_Score=65, Evalue=7e-11,
Organism=Homo sapiens, GI221136753, Length=184, Percent_Identity=29.3478260869565, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1787660, Length=264, Percent_Identity=35.9848484848485, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1787659, Length=263, Percent_Identity=31.1787072243346, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI221142681, Length=255, Percent_Identity=29.8039215686275, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1788597, Length=264, Percent_Identity=28.030303030303, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI87082183, Length=258, Percent_Identity=24.4186046511628, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788682, Length=187, Percent_Identity=30.4812834224599, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790281, Length=182, Percent_Identity=26.9230769230769, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17540714, Length=230, Percent_Identity=32.6086956521739, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17560910, Length=209, Percent_Identity=31.5789473684211, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17536985, Length=268, Percent_Identity=26.4925373134328, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI25145438, Length=241, Percent_Identity=28.2157676348548, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17535521, Length=255, Percent_Identity=27.0588235294118, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17554946, Length=254, Percent_Identity=28.3464566929134, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17534483, Length=185, Percent_Identity=29.7297297297297, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17549921, Length=196, Percent_Identity=28.0612244897959, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI25144157, Length=187, Percent_Identity=28.8770053475936, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI25144160, Length=187, Percent_Identity=28.8770053475936, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17558304, Length=166, Percent_Identity=33.1325301204819, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17540306, Length=241, Percent_Identity=28.2157676348548, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=189, Percent_Identity=25.3968253968254, Blast_Score=72, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24653139, Length=269, Percent_Identity=32.3420074349442, Blast_Score=126, Evalue=1e-29,
Organism=Drosophila melanogaster, GI20129971, Length=244, Percent_Identity=29.9180327868852, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24653477, Length=244, Percent_Identity=29.9180327868852, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21357171, Length=236, Percent_Identity=30.9322033898305, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19922422, Length=264, Percent_Identity=26.8939393939394, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24650670, Length=205, Percent_Identity=29.7560975609756, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24654903, Length=201, Percent_Identity=27.8606965174129, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28571729, Length=142, Percent_Identity=32.3943661971831, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28571730, Length=142, Percent_Identity=32.3943661971831, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19920382, Length=188, Percent_Identity=27.1276595744681, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27509; Mature: 27378

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDIVLQQLDRGLLTITMNRPDRRNALNQDMIRGLVEAAHRAKDDPQVRAVLLKGGGGTF
CCHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEE
CVGGDVKSMAAAGDPPPLEQKVANLRRGMEVSRLLHQMPKPVVAQIDGAAAGAGLSMALA
EECCCHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCEEECCCCCCCCCCEEEEE
CDLRVAGASAKITTAFAKVGLSGDFGGTYFLTHLIGAARARDLYLTSPVLTAPEALALGI
EEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHH
VSRVVPDAEVESATRDLALSLAQGPSVTLGYIKTNITNAETLSLEACFDAEALHHSRCAE
HHHHCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEEEHHHHCHHHHHHHHHHH
TADHKEAAAAFVEKRAAVFQGR
HHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TDIVLQQLDRGLLTITMNRPDRRNALNQDMIRGLVEAAHRAKDDPQVRAVLLKGGGGTF
CHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEE
CVGGDVKSMAAAGDPPPLEQKVANLRRGMEVSRLLHQMPKPVVAQIDGAAAGAGLSMALA
EECCCHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCEEECCCCCCCCCCEEEEE
CDLRVAGASAKITTAFAKVGLSGDFGGTYFLTHLIGAARARDLYLTSPVLTAPEALALGI
EEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHH
VSRVVPDAEVESATRDLALSLAQGPSVTLGYIKTNITNAETLSLEACFDAEALHHSRCAE
HHHHCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEEEHHHHCHHHHHHHHHHH
TADHKEAAAAFVEKRAAVFQGR
HHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA