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The map label for this gene is manX [C]

Identifier: 86747592

GI number: 86747592

Start: 514079

End: 514480

Strand: Direct

Name: manX [C]

Synonym: RPB_0466

Alternate gene names: 86747592

Gene position: 514079-514480 (Clockwise)

Preceding gene: 86747591

Following gene: 86747593

Centisome position: 9.64

GC content: 60.95

Gene sequence:

>402_bases
ATGATTGGTCTAGTACTTGTGACCCACGGGCGCCTTGCCGACGAGTTCAAGGCAGCGCTGGAACACGTGATGGGGCCACA
AAAGCAAATCGAAGCCGTCACGATCGGCGCCGAAGATGACGCCGACCTGTGTCGCAGCGACATCATCGAGGCGGTGAATC
GCGTCGACAGTGGTGACGGTGTTGCGATTCTGACCGACATGTTCGGCGGAACGCCGTCGAACCTTGCGATCTCCTGTATG
AGCCGCCCCAAGGTCGAAGTGCTCGCCGGGATCAATCTGCCGATGCTGGTGAAACTGGCCAAGGTCCGCGAGGAACGCGC
TTTGCCCGATGCGATCGCTCTCGCCCAGGAAGCCGGGCGCAAATACGTCACCATCGCCAGCCGGGTGCTGGCCGGAAAAT
GA

Upstream 100 bases:

>100_bases
TCGAGGGTGAATCGCGAAAACCCCCTTGCATAGGGGCTCTGGATGGTCAAAGTGACCCGTTCGCGCGGTGCACCCACGAA
GCGCCCGCGAGGAGTTTCCG

Downstream 100 bases:

>100_bases
CCGCAGACGTGCCCCAGCCCGCGACAGACGCACAGGCGGGCACCACCGCCGGAAAAATCTCCCGGGAGATTCCCGTCATC
AACAAGCGCGGCCTGCACGC

Product: PTS system fructose subfamily IIA component

Products: protein histidine; sugar phosphate

Alternate protein names: PTS System IIA Component; PTS System IIA Component Man Family; PTS System Fructose IIA Component; PTS System Mannose/Fructose/Sorbose Family IIA Subunit; IIA Component Of Sugar Transport PTS System; Pts System Fructose Subfamily IIa Component; PTS System Protein; Fructose-Specific IIA Component; PTS System Transporter Subunit IIA; Protein-N P-Phosphohistidine-Sugar Phosphotransferase; PTS System Mannose/Fructose-Specific IIA Component; Pts System Mannose-Specific Eiiab Component; PTS Mannose-Specific IIA Component; PTS System Mannose Subfamily IIA Subunit; PTS System N-Acetylgalactosamine-Specific IIA Component; PTS System Fructose-Specific Transporter Subunit IIA; Pts System Mannose/Fructose/Sorbose Family IIa Component; Phosphotransferase System Subunit; PTS System Fructose Subfamily Transprotein Subunit IIA; Pts System Fructose IIA Component; PTS System Mannose/Fructose-Specific Component IIA; Phosphotransferase System Fructose Subfamily IIA Component; Mannose-Specific Phosphotransferase System Component IIA; PTS Permease; PTS System Mannose-Specific II AB Component; PTS System Mannose-Type Transporter Subunit IIA; PTS System Mannose-Specific IIAB Component; Pentitol Phosphotransferase IIA Component; PTS System Component IIA; PTS Permease Protein; PTS System Mannose-Specific Transporter Subunit IIAB; Phosphotransferase System IIA Component; PTS System Mannose/Fructose IIA Component; Phosphotransferase Mannose/Fructose-Specific Component IIA; PTS System IIa Component; Phosphotransferase System Fructose-Specific IIA

Number of amino acids: Translated: 133; Mature: 133

Protein sequence:

>133_residues
MIGLVLVTHGRLADEFKAALEHVMGPQKQIEAVTIGAEDDADLCRSDIIEAVNRVDSGDGVAILTDMFGGTPSNLAISCM
SRPKVEVLAGINLPMLVKLAKVREERALPDAIALAQEAGRKYVTIASRVLAGK

Sequences:

>Translated_133_residues
MIGLVLVTHGRLADEFKAALEHVMGPQKQIEAVTIGAEDDADLCRSDIIEAVNRVDSGDGVAILTDMFGGTPSNLAISCM
SRPKVEVLAGINLPMLVKLAKVREERALPDAIALAQEAGRKYVTIASRVLAGK
>Mature_133_residues
MIGLVLVTHGRLADEFKAALEHVMGPQKQIEAVTIGAEDDADLCRSDIIEAVNRVDSGDGVAILTDMFGGTPSNLAISCM
SRPKVEVLAGINLPMLVKLAKVREERALPDAIALAQEAGRKYVTIASRVLAGK

Specific function: This Is A Component Of The Phosphoenolpyruvate-Dependent Sugar Phosphotransferase System (Pts), A Major Carbohydrate Active -Transport System. The IIcd Domains Contain The Sugar Binding Site And The Transmembrane Channel; The IIa Domain Contains The Prim

COG id: COG2893

COG function: function code G; Phosphotransferase system, mannose/fructose-specific component IIA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788120, Length=124, Percent_Identity=29.8387096774194, Blast_Score=61, Evalue=3e-11,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.1.69

Molecular weight: Translated: 14109; Mature: 14109

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS51096 PTS_EIIA_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGLVLVTHGRLADEFKAALEHVMGPQKQIEAVTIGAEDDADLCRSDIIEAVNRVDSGDG
CEEEEEEECCCCHHHHHHHHHHHCCCHHHEEEEEECCCCCHHHHHHHHHHHHHHCCCCCC
VAILTDMFGGTPSNLAISCMSRPKVEVLAGINLPMLVKLAKVREERALPDAIALAQEAGR
EEEEEHHCCCCCCCCEEEECCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHCC
KYVTIASRVLAGK
HHHHHHHHHHCCC
>Mature Secondary Structure
MIGLVLVTHGRLADEFKAALEHVMGPQKQIEAVTIGAEDDADLCRSDIIEAVNRVDSGDG
CEEEEEEECCCCHHHHHHHHHHHCCCHHHEEEEEECCCCCHHHHHHHHHHHHHHCCCCCC
VAILTDMFGGTPSNLAISCMSRPKVEVLAGINLPMLVKLAKVREERALPDAIALAQEAGR
EEEEEHHCCCCCCCCEEEECCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHCC
KYVTIASRVLAGK
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA