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The map label for this gene is sucB [H]

Identifier: 86747403

GI number: 86747403

Start: 311163

End: 312398

Strand: Reverse

Name: sucB [H]

Synonym: RPB_0277

Alternate gene names: 86747403

Gene position: 312398-311163 (Counterclockwise)

Preceding gene: 86747404

Following gene: 86747402

Centisome position: 5.86

GC content: 67.96

Gene sequence:

>1236_bases
ATGACTGAAATTCGCGTTCCGACGCTCGGCGAATCCGTGACCGAGGCGACCATCGGCCGCTGGTTCAAGAAGCAGGGTGA
CGCCGTCGCGGTCGACGAGCCGCTGGTCGAACTCGAGACCGACAAGGTCACCATCGAAGTGCCGGCGCCGTCGGCCGGCA
CCCTCGGCGAAATCATCGCCAAGGACGGCGAGACCGTCGCCGTCGGCGCGCTGCTGGGCCAGATCTCGGAAGGGGGCGGT
GCCGCCAAGCCCGCCGCGCCGGCCAAGCCGGCCGCCGCGCCGGCTGCCGCCGCTGCAGCGCCCGCTGCGGCCGCGCCCAA
GGCCGCGCCGGCCGATGCGCCGCAGGCACCGTCGGTGCGGCGGCTGTCGACCGAGAGCGGCGTCGATGCCTCGACCGTGC
CGGGCTCCGGCAAGGACGGCCGCGTCACCAAGGGCGACATGCTGGCGGCGATCGAGAAGGCGGCGTCGGCGCCGACCCCG
GTCAACCAGCCCGCCGCCGCCGTCCAGGTCCGCGCCCCGTCGCCGGCCGACGATGCCGCGCGCGAGGAGCGCGTCAAGAT
GACCCGGCTGCGCCAGACCATCGCGCGCCGGCTCAAGGAAGTTCAGAACACCGCCGCGATGCTGACGACCTTCAACGAGG
TCGACATGACCAACGTCATGGCGCTGCGCAGCCAATACAAGGACGTGTTCGAGAAGAAGCACGGCGCCAAGCTCGGCTTC
ATGGGCTTCTTCACAAAGGCCTGCGTGCAAGCGCTGAAGGACATCCCGGCGGTCAATGCCGAGATCGACGGCACCGATTT
GATCTACAAGAACTACTACCATGTCGGCGTCGCGGTCGGCACCGACAAGGGCCTGGTGGTGCCGGTGGTGCGCGATTGCG
ACGAGAAGTCGATCGCCGCGATCGAGAAGGGCATTGCCGATTTCGGCAAGCGCGCCCGCGACGGCCAGCTCAAGATCGAG
GAAATGCAGGGCGGCACCTTCACGATCACCAATGGCGGCATCTACGGCTCGCTGATGTCGACGCCGATCCTCAACGCGCC
GCAGTCGGCGATCCTCGGCATGCACAAGATCCAGGAGCGGCCGGTGGTCGTCGCCGGCAAGATCGAAATCCGGCCGATGA
TGTATCTGGCGCTCAGCTACGATCACCGCGTGATCGACGGCAAGGAAGCGGTGACCTTCCTGGTCCGCGTCAAGGAAAGC
CTGGAAGACCCGGCCCGGCTGGTGCTGGATCTGTAA

Upstream 100 bases:

>100_bases
CGCCACGGGCCGCCGCGCGCCGGCGGCCGGTGTCGTTCGAGACATTTGGATCAGGGGCGAGCCGCCGCAGCAGCGGTGAC
GCCGCAAGAGGACAAAAACG

Downstream 100 bases:

>100_bases
GAGCGACGTCGACACGGGCAGGAAAGCCGGCGTTTCGTCGAAGCGGATTGGGGGGACATGATGCAGCCGATGCCGACCGA
GATCGTGGTGCTGGGCTGGA

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MTEIRVPTLGESVTEATIGRWFKKQGDAVAVDEPLVELETDKVTIEVPAPSAGTLGEIIAKDGETVAVGALLGQISEGGG
AAKPAAPAKPAAAPAAAAAAPAAAAPKAAPADAPQAPSVRRLSTESGVDASTVPGSGKDGRVTKGDMLAAIEKAASAPTP
VNQPAAAVQVRAPSPADDAAREERVKMTRLRQTIARRLKEVQNTAAMLTTFNEVDMTNVMALRSQYKDVFEKKHGAKLGF
MGFFTKACVQALKDIPAVNAEIDGTDLIYKNYYHVGVAVGTDKGLVVPVVRDCDEKSIAAIEKGIADFGKRARDGQLKIE
EMQGGTFTITNGGIYGSLMSTPILNAPQSAILGMHKIQERPVVVAGKIEIRPMMYLALSYDHRVIDGKEAVTFLVRVKES
LEDPARLVLDL

Sequences:

>Translated_411_residues
MTEIRVPTLGESVTEATIGRWFKKQGDAVAVDEPLVELETDKVTIEVPAPSAGTLGEIIAKDGETVAVGALLGQISEGGG
AAKPAAPAKPAAAPAAAAAAPAAAAPKAAPADAPQAPSVRRLSTESGVDASTVPGSGKDGRVTKGDMLAAIEKAASAPTP
VNQPAAAVQVRAPSPADDAAREERVKMTRLRQTIARRLKEVQNTAAMLTTFNEVDMTNVMALRSQYKDVFEKKHGAKLGF
MGFFTKACVQALKDIPAVNAEIDGTDLIYKNYYHVGVAVGTDKGLVVPVVRDCDEKSIAAIEKGIADFGKRARDGQLKIE
EMQGGTFTITNGGIYGSLMSTPILNAPQSAILGMHKIQERPVVVAGKIEIRPMMYLALSYDHRVIDGKEAVTFLVRVKES
LEDPARLVLDL
>Mature_410_residues
TEIRVPTLGESVTEATIGRWFKKQGDAVAVDEPLVELETDKVTIEVPAPSAGTLGEIIAKDGETVAVGALLGQISEGGGA
AKPAAPAKPAAAPAAAAAAPAAAAPKAAPADAPQAPSVRRLSTESGVDASTVPGSGKDGRVTKGDMLAAIEKAASAPTPV
NQPAAAVQVRAPSPADDAAREERVKMTRLRQTIARRLKEVQNTAAMLTTFNEVDMTNVMALRSQYKDVFEKKHGAKLGFM
GFFTKACVQALKDIPAVNAEIDGTDLIYKNYYHVGVAVGTDKGLVVPVVRDCDEKSIAAIEKGIADFGKRARDGQLKIEE
MQGGTFTITNGGIYGSLMSTPILNAPQSAILGMHKIQERPVVVAGKIEIRPMMYLALSYDHRVIDGKEAVTFLVRVKESL
EDPARLVLDL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and l

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=251, Percent_Identity=54.5816733067729, Blast_Score=292, Evalue=3e-79,
Organism=Homo sapiens, GI203098753, Length=455, Percent_Identity=27.9120879120879, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI203098816, Length=455, Percent_Identity=27.9120879120879, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI31711992, Length=434, Percent_Identity=27.1889400921659, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI110671329, Length=430, Percent_Identity=28.3720930232558, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI260898739, Length=160, Percent_Identity=36.875, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI1786946, Length=409, Percent_Identity=50.1222493887531, Blast_Score=396, Evalue=1e-111,
Organism=Escherichia coli, GI1786305, Length=429, Percent_Identity=35.6643356643357, Blast_Score=190, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI25146366, Length=408, Percent_Identity=44.3627450980392, Blast_Score=331, Evalue=4e-91,
Organism=Caenorhabditis elegans, GI17560088, Length=447, Percent_Identity=30.8724832214765, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17537937, Length=427, Percent_Identity=26.2295081967213, Blast_Score=165, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17538894, Length=316, Percent_Identity=28.7974683544304, Blast_Score=124, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6320352, Length=414, Percent_Identity=45.4106280193237, Blast_Score=341, Evalue=1e-94,
Organism=Saccharomyces cerevisiae, GI6324258, Length=450, Percent_Identity=26.8888888888889, Blast_Score=138, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24645909, Length=249, Percent_Identity=55.421686746988, Blast_Score=285, Evalue=5e-77,
Organism=Drosophila melanogaster, GI18859875, Length=421, Percent_Identity=30.4038004750594, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI20129315, Length=233, Percent_Identity=31.3304721030043, Blast_Score=118, Evalue=9e-27,
Organism=Drosophila melanogaster, GI24582497, Length=233, Percent_Identity=31.3304721030043, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 43244; Mature: 43113

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEIRVPTLGESVTEATIGRWFKKQGDAVAVDEPLVELETDKVTIEVPAPSAGTLGEIIA
CCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCEEEECCEEEEEECCCCCCCHHHHHH
KDGETVAVGALLGQISEGGGAAKPAAPAKPAAAPAAAAAAPAAAAPKAAPADAPQAPSVR
CCCCEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCHH
RLSTESGVDASTVPGSGKDGRVTKGDMLAAIEKAASAPTPVNQPAAAVQVRAPSPADDAA
EECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCHHHH
REERVKMTRLRQTIARRLKEVQNTAAMLTTFNEVDMTNVMALRSQYKDVFEKKHGAKLGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCH
MGFFTKACVQALKDIPAVNAEIDGTDLIYKNYYHVGVAVGTDKGLVVPVVRDCDEKSIAA
HHHHHHHHHHHHHHCCCCCCCCCCCEEEEECEEEEEEEEECCCCEEEEHHHCCCHHHHHH
IEKGIADFGKRARDGQLKIEEMQGGTFTITNGGIYGSLMSTPILNAPQSAILGMHKIQER
HHHHHHHHHHCCCCCCEEEEEECCCEEEEECCCEEEHHHHCCCCCCCHHHHHHHHHHCCC
PVVVAGKIEIRPMMYLALSYDHRVIDGKEAVTFLVRVKESLEDPARLVLDL
CEEEEEEEEECEEEEEEEECCCEEECCHHHHHHHHHHHHHHCCCHHHEECC
>Mature Secondary Structure 
TEIRVPTLGESVTEATIGRWFKKQGDAVAVDEPLVELETDKVTIEVPAPSAGTLGEIIA
CCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCEEEECCEEEEEECCCCCCCHHHHHH
KDGETVAVGALLGQISEGGGAAKPAAPAKPAAAPAAAAAAPAAAAPKAAPADAPQAPSVR
CCCCEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCHH
RLSTESGVDASTVPGSGKDGRVTKGDMLAAIEKAASAPTPVNQPAAAVQVRAPSPADDAA
EECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCHHHH
REERVKMTRLRQTIARRLKEVQNTAAMLTTFNEVDMTNVMALRSQYKDVFEKKHGAKLGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCH
MGFFTKACVQALKDIPAVNAEIDGTDLIYKNYYHVGVAVGTDKGLVVPVVRDCDEKSIAA
HHHHHHHHHHHHHHCCCCCCCCCCCEEEEECEEEEEEEEECCCCEEEEHHHCCCHHHHHH
IEKGIADFGKRARDGQLKIEEMQGGTFTITNGGIYGSLMSTPILNAPQSAILGMHKIQER
HHHHHHHHHHCCCCCCEEEEEECCCEEEEECCCEEEHHHHCCCCCCCHHHHHHHHHHCCC
PVVVAGKIEIRPMMYLALSYDHRVIDGKEAVTFLVRVKESLEDPARLVLDL
CEEEEEEEEECEEEEEEEECCCEEECCHHHHHHHHHHHHHHCCCHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12874367 [H]