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The map label for this gene is priA [H]

Identifier: 86747396

GI number: 86747396

Start: 303801

End: 306008

Strand: Reverse

Name: priA [H]

Synonym: RPB_0270

Alternate gene names: 86747396

Gene position: 306008-303801 (Counterclockwise)

Preceding gene: 86747399

Following gene: 86747394

Centisome position: 5.74

GC content: 68.93

Gene sequence:

>2208_bases
ATGGACGACTCTCCCACCAGCCCGGCCTCGCCCTCGGCAATGCGCGTCGTCGACGTGCAGGTGCCGGTCGCGATCGATCA
GGCCTATTCGTACCGGGTGCCGCGCGGGCTCGAGCTGAAGCCCGGCGACGTCGTCGCGGTGCCGCTCGGGTCGCGCGAGG
TGATGGGGGTGGTCTGGGCCGAGAACGAAAATCCCGATCCGCGGCTGCACAATCGCCTCAAGGATGTTGCCGAGAAGCTC
GACGTGCCGCCGCTGCGCGAGGAGCTGCGCCGGCTGGTCGACTGGGTCGCCAATTACACGCTGTCGCCGCGCGGCCAGGT
GCTGCGGATGACGCTGCGGATGGGCGAACTCGGCCCGGAGCGGGTGCGGACGGGAGTCCGCCTGATCGGCCCCCCGCCGC
AGCGGATGACGCCGGCGCGGCGGCGGCTGATCGAGATCCTGTCCGACGGCCTGCTGCACGGCAAATCGGACGCGGCGAAG
GAGGCGGGCTGCAGCGCCGGGGTGATCGACGGGCTGGTCGACGAGGGCACGCTCAGCGTCGAGCCGATGCCGCGCGAGCC
GGCGGCGCCGACGCCCGATCCGGATTTTGCGCCGGCGGATTTCTCGCCGGAGCAGGCGGCGGCGGCCGAGACGATGCGCG
AACTGGTCAAGTCCGGCGGCGGCTTTCAGGCGGCGCTGCTCGACGGCGTCACCGGCTCCGGCAAGACGGAAGTGTATTTC
GAGGCGGTCGCCGAAATCATCCGCCAGGGGCGGCAGACGCTGATCCTGATGCCGGAGATCGCGCTGACCGGGCAATTCCT
CGACCGCTTCGCGCTGCGCTTCGGCGTCCGGCCGCTGGAATGGCATTCTGAACTCACGCCGCGCACCCGCGCCCGCAACT
GGGCGGCGATCGCCGCCGGCGAGGCGCAGGTGGTGGTCGGGGCGCGTTCGGCGCTGTTCCTGCCTTACGCCGATCTCGGG
CTGATCATCGTCGATGAGGAGCACGACCAGGCCTACAAGCAGGAGGACGGCGCGCATTATCACGCCCGCGACATGGCGGT
GGTGCGGGCGTCGATCGCGAAAATTCCGATCGTGCTGGCCTCGGCGACGCCGTCGGTCGAGACCGAGGTCAACGCCCGCA
AGGGCCGCTACAGGCGGATTCCGCTGCCGTCACGATTCGGCGGCCAGCACATGCCGCAGATCGAGGCGATCGATCTGCGC
CGCGCGCCGCCGATGCGCGGGCGCTTCATCTCGCCGCCGCTGGCCGAGCAGATCGGCCACGCCATCGAACGCGGCGAACA
GGCGCTGCTGTTTCTCAACCGCCGCGGCTATGCGCCGCTGACCTTGTGCCGGGCCTGCGGTCACCGCTTCGCCTGCACCA
TCTGCGACGCCTGGCTGGTCGATCACCGCTTCCGGCAGCGGCTGGTGTGCCACCATTGCGGCTTCTCGATGCCGCGCCCG
CACCAATGCCCGCATTGCGGCGCCGAGGAATCTTTAGTCGCAGTCGGGCCCGGCGTCGAACGGCTGCAGGAGGAGGCGGC
CAGCCTGTTTCCGCAGGCGCGCACCATGGTGCTGTCGAGCGACCTGATCACCTCGATCGAGGCGATGCGCAGCGAACTCA
ACGATATCGCCGAGGGCCGGGTCGACATCATCATCGGCACCCAGCTTGTGGCGAAAGGTCACAACTTCCCGCGGCTGAAC
CTGGTCGGTGTGGTCGATGCCGATCTCGGCCTGAGCAATGGCGATCCGCGCGCCGCCGAGCGCACCTTCCAATTGCTCAA
TCAGGTGATCGGCCGCGCCGGCCGCGATCAGGGCCGCGGCGTCGGCTTTCTGCAGACGCATCAGCCGGAGCATCCGGTGA
TGAAGGCGCTGGTCGCGTGCGACCGCGAGGCTTTCTATGCCAGCGAGATCGACGCCCGCGAACGCACGTTGTATCCGCCG
TTCGGCCGGCTCGCGAGCCTGATCGTGTCCGCCGGCGACCGGCCGAGTGCGGAAGGCTTTGCGCGGAAGCTGGCGTCCTG
CGCGCCGGTGGACGAGCGCGTCCAGGTGCTGGGACCGGCGGAAGCGCCGCTGGCGGTGATCAAAGGCCGCTATCGCTTTC
GGCTGCTGGTGAAGTCGGTGCGGTCGTTCGATTTGTCGAACTACTTGCGGTCATGGCTGGCGCAAGGACCGAAGACGAAA
GGAAACCTGAAGCTCGAGGTCGACGTCGATCCGCAAAGCTTCCTGTAA

Upstream 100 bases:

>100_bases
TCGTCGCTTCGCTCCTCGCAATGACGCGGAGGTTGTGGCCCGATCATAGCCGATTTCCTTTCCCATTGATGAAAGGCCGC
TTTAGCGGCACACCGGCGCG

Downstream 100 bases:

>100_bases
TCTTCCTTCGCGTCGGCGGGAGCGCGCGTGTCACAAAAACGAAAGCCTGCCGTCATCGCTGACGGCAGGCTGCAGTTCGG
GCTCGCGACGATCGCGGCCT

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 735; Mature: 735

Protein sequence:

>735_residues
MDDSPTSPASPSAMRVVDVQVPVAIDQAYSYRVPRGLELKPGDVVAVPLGSREVMGVVWAENENPDPRLHNRLKDVAEKL
DVPPLREELRRLVDWVANYTLSPRGQVLRMTLRMGELGPERVRTGVRLIGPPPQRMTPARRRLIEILSDGLLHGKSDAAK
EAGCSAGVIDGLVDEGTLSVEPMPREPAAPTPDPDFAPADFSPEQAAAAETMRELVKSGGGFQAALLDGVTGSGKTEVYF
EAVAEIIRQGRQTLILMPEIALTGQFLDRFALRFGVRPLEWHSELTPRTRARNWAAIAAGEAQVVVGARSALFLPYADLG
LIIVDEEHDQAYKQEDGAHYHARDMAVVRASIAKIPIVLASATPSVETEVNARKGRYRRIPLPSRFGGQHMPQIEAIDLR
RAPPMRGRFISPPLAEQIGHAIERGEQALLFLNRRGYAPLTLCRACGHRFACTICDAWLVDHRFRQRLVCHHCGFSMPRP
HQCPHCGAEESLVAVGPGVERLQEEAASLFPQARTMVLSSDLITSIEAMRSELNDIAEGRVDIIIGTQLVAKGHNFPRLN
LVGVVDADLGLSNGDPRAAERTFQLLNQVIGRAGRDQGRGVGFLQTHQPEHPVMKALVACDREAFYASEIDARERTLYPP
FGRLASLIVSAGDRPSAEGFARKLASCAPVDERVQVLGPAEAPLAVIKGRYRFRLLVKSVRSFDLSNYLRSWLAQGPKTK
GNLKLEVDVDPQSFL

Sequences:

>Translated_735_residues
MDDSPTSPASPSAMRVVDVQVPVAIDQAYSYRVPRGLELKPGDVVAVPLGSREVMGVVWAENENPDPRLHNRLKDVAEKL
DVPPLREELRRLVDWVANYTLSPRGQVLRMTLRMGELGPERVRTGVRLIGPPPQRMTPARRRLIEILSDGLLHGKSDAAK
EAGCSAGVIDGLVDEGTLSVEPMPREPAAPTPDPDFAPADFSPEQAAAAETMRELVKSGGGFQAALLDGVTGSGKTEVYF
EAVAEIIRQGRQTLILMPEIALTGQFLDRFALRFGVRPLEWHSELTPRTRARNWAAIAAGEAQVVVGARSALFLPYADLG
LIIVDEEHDQAYKQEDGAHYHARDMAVVRASIAKIPIVLASATPSVETEVNARKGRYRRIPLPSRFGGQHMPQIEAIDLR
RAPPMRGRFISPPLAEQIGHAIERGEQALLFLNRRGYAPLTLCRACGHRFACTICDAWLVDHRFRQRLVCHHCGFSMPRP
HQCPHCGAEESLVAVGPGVERLQEEAASLFPQARTMVLSSDLITSIEAMRSELNDIAEGRVDIIIGTQLVAKGHNFPRLN
LVGVVDADLGLSNGDPRAAERTFQLLNQVIGRAGRDQGRGVGFLQTHQPEHPVMKALVACDREAFYASEIDARERTLYPP
FGRLASLIVSAGDRPSAEGFARKLASCAPVDERVQVLGPAEAPLAVIKGRYRFRLLVKSVRSFDLSNYLRSWLAQGPKTK
GNLKLEVDVDPQSFL
>Mature_735_residues
MDDSPTSPASPSAMRVVDVQVPVAIDQAYSYRVPRGLELKPGDVVAVPLGSREVMGVVWAENENPDPRLHNRLKDVAEKL
DVPPLREELRRLVDWVANYTLSPRGQVLRMTLRMGELGPERVRTGVRLIGPPPQRMTPARRRLIEILSDGLLHGKSDAAK
EAGCSAGVIDGLVDEGTLSVEPMPREPAAPTPDPDFAPADFSPEQAAAAETMRELVKSGGGFQAALLDGVTGSGKTEVYF
EAVAEIIRQGRQTLILMPEIALTGQFLDRFALRFGVRPLEWHSELTPRTRARNWAAIAAGEAQVVVGARSALFLPYADLG
LIIVDEEHDQAYKQEDGAHYHARDMAVVRASIAKIPIVLASATPSVETEVNARKGRYRRIPLPSRFGGQHMPQIEAIDLR
RAPPMRGRFISPPLAEQIGHAIERGEQALLFLNRRGYAPLTLCRACGHRFACTICDAWLVDHRFRQRLVCHHCGFSMPRP
HQCPHCGAEESLVAVGPGVERLQEEAASLFPQARTMVLSSDLITSIEAMRSELNDIAEGRVDIIIGTQLVAKGHNFPRLN
LVGVVDADLGLSNGDPRAAERTFQLLNQVIGRAGRDQGRGVGFLQTHQPEHPVMKALVACDREAFYASEIDARERTLYPP
FGRLASLIVSAGDRPSAEGFARKLASCAPVDERVQVLGPAEAPLAVIKGRYRFRLLVKSVRSFDLSNYLRSWLAQGPKTK
GNLKLEVDVDPQSFL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=756, Percent_Identity=34.3915343915344, Blast_Score=378, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD [H]

EC number: NA

Molecular weight: Translated: 80652; Mature: 80652

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDSPTSPASPSAMRVVDVQVPVAIDQAYSYRVPRGLELKPGDVVAVPLGSREVMGVVWA
CCCCCCCCCCCCCEEEEEEEECEEECCHHHCCCCCCCCCCCCCEEEEECCCCEEEEEEEE
ENENPDPRLHNRLKDVAEKLDVPPLREELRRLVDWVANYTLSPRGQVLRMTLRMGELGPE
CCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHH
RVRTGVRLIGPPPQRMTPARRRLIEILSDGLLHGKSDAAKEAGCSAGVIDGLVDEGTLSV
HHHCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHCCCCCHHHHHHHCCCCEEE
EPMPREPAAPTPDPDFAPADFSPEQAAAAETMRELVKSGGGFQAALLDGVTGSGKTEVYF
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHH
EAVAEIIRQGRQTLILMPEIALTGQFLDRFALRFGVRPLEWHSELTPRTRARNWAAIAAG
HHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHCCCEEEEECC
EAQVVVGARSALFLPYADLGLIIVDEEHDQAYKQEDGAHYHARDMAVVRASIAKIPIVLA
CEEEEEECCCEEEEEECCCCEEEEECCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCEEEE
SATPSVETEVNARKGRYRRIPLPSRFGGQHMPQIEAIDLRRAPPMRGRFISPPLAEQIGH
CCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHH
AIERGEQALLFLNRRGYAPLTLCRACGHRFACTICDAWLVDHRFRQRLVCHHCGFSMPRP
HHHCCCEEEEEEECCCCCHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
HQCPHCGAEESLVAVGPGVERLQEEAASLFPQARTMVLSSDLITSIEAMRSELNDIAEGR
CCCCCCCCCCCEEEECCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VDIIIGTQLVAKGHNFPRLNLVGVVDADLGLSNGDPRAAERTFQLLNQVIGRAGRDQGRG
EEEEECCCHHHCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
VGFLQTHQPEHPVMKALVACDREAFYASEIDARERTLYPPFGRLASLIVSAGDRPSAEGF
CCEECCCCCCHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHH
ARKLASCAPVDERVQVLGPAEAPLAVIKGRYRFRLLVKSVRSFDLSNYLRSWLAQGPKTK
HHHHHHCCCHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCC
GNLKLEVDVDPQSFL
CCEEEEEECCHHHCC
>Mature Secondary Structure
MDDSPTSPASPSAMRVVDVQVPVAIDQAYSYRVPRGLELKPGDVVAVPLGSREVMGVVWA
CCCCCCCCCCCCCEEEEEEEECEEECCHHHCCCCCCCCCCCCCEEEEECCCCEEEEEEEE
ENENPDPRLHNRLKDVAEKLDVPPLREELRRLVDWVANYTLSPRGQVLRMTLRMGELGPE
CCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHH
RVRTGVRLIGPPPQRMTPARRRLIEILSDGLLHGKSDAAKEAGCSAGVIDGLVDEGTLSV
HHHCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHCCCCCHHHHHHHCCCCEEE
EPMPREPAAPTPDPDFAPADFSPEQAAAAETMRELVKSGGGFQAALLDGVTGSGKTEVYF
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHH
EAVAEIIRQGRQTLILMPEIALTGQFLDRFALRFGVRPLEWHSELTPRTRARNWAAIAAG
HHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHCCCEEEEECC
EAQVVVGARSALFLPYADLGLIIVDEEHDQAYKQEDGAHYHARDMAVVRASIAKIPIVLA
CEEEEEECCCEEEEEECCCCEEEEECCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCEEEE
SATPSVETEVNARKGRYRRIPLPSRFGGQHMPQIEAIDLRRAPPMRGRFISPPLAEQIGH
CCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHH
AIERGEQALLFLNRRGYAPLTLCRACGHRFACTICDAWLVDHRFRQRLVCHHCGFSMPRP
HHHCCCEEEEEEECCCCCHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
HQCPHCGAEESLVAVGPGVERLQEEAASLFPQARTMVLSSDLITSIEAMRSELNDIAEGR
CCCCCCCCCCCEEEECCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VDIIIGTQLVAKGHNFPRLNLVGVVDADLGLSNGDPRAAERTFQLLNQVIGRAGRDQGRG
EEEEECCCHHHCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
VGFLQTHQPEHPVMKALVACDREAFYASEIDARERTLYPPFGRLASLIVSAGDRPSAEGF
CCEECCCCCCHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHH
ARKLASCAPVDERVQVLGPAEAPLAVIKGRYRFRLLVKSVRSFDLSNYLRSWLAQGPKTK
HHHHHHCCCHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCC
GNLKLEVDVDPQSFL
CCEEEEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2861810 [H]