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The map label for this gene is hemN [H]

Identifier: 86747291

GI number: 86747291

Start: 176722

End: 178146

Strand: Reverse

Name: hemN [H]

Synonym: RPB_0164

Alternate gene names: 86747291

Gene position: 178146-176722 (Counterclockwise)

Preceding gene: 86747296

Following gene: 86747290

Centisome position: 3.34

GC content: 70.39

Gene sequence:

>1425_bases
ATGACGATGGCGCGTGCGACGACGAGCGGCGGTGCGCCGCATGGCGGACACGGGCATCATCCGGCGGCGGCCGCGGCGTT
GCAGATCAGCGATTACTTCGTGCGGATCGGCTGCGATGCCTTGACCGAGGCGTTCGCGAAGCGCTCATTCTCGCCGCCAT
GGCGCGGCAGCCGGCCGGTCGACGGCGACGATGTCGAGGCGGTGCTCGCGCGGATCTTCGCGACGCTCCGCAACGAGACC
GCGGTCGCCTATGTGCACGTGCCGTTCTGCCAGACGCATTGCCTGTTCTGCGGCTTCTTCCAGAACGTCTGGCGCGCCGA
GGCCGGTCCCGCTTATGTTGACGACGTGCTCGCCGAACTCGCGGCGCGGTCCTCCACCCCGCTGATCGCGTCGGCGCCGA
TCGACGCGGTGTATCTCGGCGGCGGCACGCCGTCGGCGCTCGCGGCCGACGATCTCGCGCGCCTGGTCGAGGGCCTGCGC
CGCTATCTGCCGCTGACGAGCGACTGCGAGATCACGCTCGAAGGTCGCGGCTTCGGCTTCGATCTGGCGAAGGCCGAGAA
GGTGGCGGACGCCGGCGTGACCCGGCTGTCGATTGGGGTGCAGACGTTCTCGACGCAGGTGCGGCGCCGGCTCGGCCGCA
AGCTCGCGGGTCCCGAGGTGCAGTCGTTTCTCGCCGATCTGGTGGCGCTCGGCCGCACCGCGGTGGTGTGCGATCTGATC
TACGGCCTGCCGGGACAGACCGACGACATTTGGGCGAACGATATCGAGATCGTCGATCGGCTCGGGCTCGACGGCGTCAC
GCTCTATGCGCTCAATATGTTTCCCGGCGGGCCGATGGCGCGCGCGATCGAGAACGGCAAGCTGGCGCCGCCGGCGGCGC
CCGGCGTGCAGGCGCGCGTTTATGCGGAAGCGGTCGAGCGGCTGGCGGGGCGGGGCTGGCTGCAGGTGTCGCAGTCGCAC
CTCGTGCGCTCGGCGCGCGAGTTCAACCGCTACAATGCGGCGATCAAGCGCGGCGTCGCCTGCCTGCCGTTCGGGGCGGG
TGCGGGCGGCCAGGCTCATGGCTATCGCTGGCGCAACGTCATCGACATCGCGCAACGCCGCGACATGATCGCGCAGCAGC
GTGCGCCGGTCGAAGGGCTGGCGCAGGTGCCGGCCGATCACGCCGCCCACGCGATGATCGCGGCCGGCCTCGAAGCCGGA
CGTCTCGACCTCGCCGCGATCGAGGCGCTGCGGCCGGGTTTCCGCGCAGCGGTGGCGCCGCTGCTGGCGAATTGGGCGGC
GGGCGGCCTCGGCGAACTCAGCCACGACGGTTTTAGGCCCAATCGCGCCGGTTCGTTCTGGATCAGCAATCTGACCAGCG
GACTGTCCGCCGGACTGCAATCGGCGACGCAGGCTGCGACATCGGGCTGCTGCGCATCTGCGTGA

Upstream 100 bases:

>100_bases
TGGCCAGTTACCTGCGTGCGCATCTGCGCGCGCTCGTGCGCGGACCCGCATCGTGGCCGATCGCGATCGATGCGGTCGCT
TCCGGTGCGAGGCGACGCCG

Downstream 100 bases:

>100_bases
CGTCGGCTATGCTGCGAACACGCAAGCGGGTCGACCGCGTCTCTGACTGATCGGGTTGGTATGAAAGTTCTGATCTTCGG
CGCGACCGGCAGGACCGGTC

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MTMARATTSGGAPHGGHGHHPAAAAALQISDYFVRIGCDALTEAFAKRSFSPPWRGSRPVDGDDVEAVLARIFATLRNET
AVAYVHVPFCQTHCLFCGFFQNVWRAEAGPAYVDDVLAELAARSSTPLIASAPIDAVYLGGGTPSALAADDLARLVEGLR
RYLPLTSDCEITLEGRGFGFDLAKAEKVADAGVTRLSIGVQTFSTQVRRRLGRKLAGPEVQSFLADLVALGRTAVVCDLI
YGLPGQTDDIWANDIEIVDRLGLDGVTLYALNMFPGGPMARAIENGKLAPPAAPGVQARVYAEAVERLAGRGWLQVSQSH
LVRSAREFNRYNAAIKRGVACLPFGAGAGGQAHGYRWRNVIDIAQRRDMIAQQRAPVEGLAQVPADHAAHAMIAAGLEAG
RLDLAAIEALRPGFRAAVAPLLANWAAGGLGELSHDGFRPNRAGSFWISNLTSGLSAGLQSATQAATSGCCASA

Sequences:

>Translated_474_residues
MTMARATTSGGAPHGGHGHHPAAAAALQISDYFVRIGCDALTEAFAKRSFSPPWRGSRPVDGDDVEAVLARIFATLRNET
AVAYVHVPFCQTHCLFCGFFQNVWRAEAGPAYVDDVLAELAARSSTPLIASAPIDAVYLGGGTPSALAADDLARLVEGLR
RYLPLTSDCEITLEGRGFGFDLAKAEKVADAGVTRLSIGVQTFSTQVRRRLGRKLAGPEVQSFLADLVALGRTAVVCDLI
YGLPGQTDDIWANDIEIVDRLGLDGVTLYALNMFPGGPMARAIENGKLAPPAAPGVQARVYAEAVERLAGRGWLQVSQSH
LVRSAREFNRYNAAIKRGVACLPFGAGAGGQAHGYRWRNVIDIAQRRDMIAQQRAPVEGLAQVPADHAAHAMIAAGLEAG
RLDLAAIEALRPGFRAAVAPLLANWAAGGLGELSHDGFRPNRAGSFWISNLTSGLSAGLQSATQAATSGCCASA
>Mature_473_residues
TMARATTSGGAPHGGHGHHPAAAAALQISDYFVRIGCDALTEAFAKRSFSPPWRGSRPVDGDDVEAVLARIFATLRNETA
VAYVHVPFCQTHCLFCGFFQNVWRAEAGPAYVDDVLAELAARSSTPLIASAPIDAVYLGGGTPSALAADDLARLVEGLRR
YLPLTSDCEITLEGRGFGFDLAKAEKVADAGVTRLSIGVQTFSTQVRRRLGRKLAGPEVQSFLADLVALGRTAVVCDLIY
GLPGQTDDIWANDIEIVDRLGLDGVTLYALNMFPGGPMARAIENGKLAPPAAPGVQARVYAEAVERLAGRGWLQVSQSHL
VRSAREFNRYNAAIKRGVACLPFGAGAGGQAHGYRWRNVIDIAQRRDMIAQQRAPVEGLAQVPADHAAHAMIAAGLEAGR
LDLAAIEALRPGFRAAVAPLLANWAAGGLGELSHDGFRPNRAGSFWISNLTSGLSAGLQSATQAATSGCCASA

Specific function: Anaerobic transformation of coproporphyrinogen-III into protoporphyrinogen-IX [H]

COG id: COG0635

COG function: function code H; Coproporphyrinogen III oxidase and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anaerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Homo sapiens, GI8922911, Length=271, Percent_Identity=27.6752767527675, Blast_Score=92, Evalue=9e-19,
Organism=Escherichia coli, GI87082341, Length=396, Percent_Identity=26.010101010101, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI1789325, Length=273, Percent_Identity=27.8388278388278, Blast_Score=98, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR004559
- InterPro:   IPR010723
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06969 HemN_C; PF04055 Radical_SAM [H]

EC number: =1.3.99.22 [H]

Molecular weight: Translated: 49946; Mature: 49815

Theoretical pI: Translated: 7.52; Mature: 7.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMARATTSGGAPHGGHGHHPAAAAALQISDYFVRIGCDALTEAFAKRSFSPPWRGSRPV
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCC
DGDDVEAVLARIFATLRNETAVAYVHVPFCQTHCLFCGFFQNVWRAEAGPAYVDDVLAEL
CCCHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
AARSSTPLIASAPIDAVYLGGGTPSALAADDLARLVEGLRRYLPLTSDCEITLEGRGFGF
HHCCCCCEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCC
DLAKAEKVADAGVTRLSIGVQTFSTQVRRRLGRKLAGPEVQSFLADLVALGRTAVVCDLI
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHH
YGLPGQTDDIWANDIEIVDRLGLDGVTLYALNMFPGGPMARAIENGKLAPPAAPGVQARV
HCCCCCCCCCCCCHHHHHHHCCCCCEEEEEEECCCCCHHHHHHCCCCCCCCCCCCCHHHH
YAEAVERLAGRGWLQVSQSHLVRSAREFNRYNAAIKRGVACLPFGAGAGGQAHGYRWRNV
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHH
IDIAQRRDMIAQQRAPVEGLAQVPADHAAHAMIAAGLEAGRLDLAAIEALRPGFRAAVAP
HHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHH
LLANWAAGGLGELSHDGFRPNRAGSFWISNLTSGLSAGLQSATQAATSGCCASA
HHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TMARATTSGGAPHGGHGHHPAAAAALQISDYFVRIGCDALTEAFAKRSFSPPWRGSRPV
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCC
DGDDVEAVLARIFATLRNETAVAYVHVPFCQTHCLFCGFFQNVWRAEAGPAYVDDVLAEL
CCCHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
AARSSTPLIASAPIDAVYLGGGTPSALAADDLARLVEGLRRYLPLTSDCEITLEGRGFGF
HHCCCCCEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCC
DLAKAEKVADAGVTRLSIGVQTFSTQVRRRLGRKLAGPEVQSFLADLVALGRTAVVCDLI
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHH
YGLPGQTDDIWANDIEIVDRLGLDGVTLYALNMFPGGPMARAIENGKLAPPAAPGVQARV
HCCCCCCCCCCCCHHHHHHHCCCCCEEEEEEECCCCCHHHHHHCCCCCCCCCCCCCHHHH
YAEAVERLAGRGWLQVSQSHLVRSAREFNRYNAAIKRGVACLPFGAGAGGQAHGYRWRNV
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHH
IDIAQRRDMIAQQRAPVEGLAQVPADHAAHAMIAAGLEAGRLDLAAIEALRPGFRAAVAP
HHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHH
LLANWAAGGLGELSHDGFRPNRAGSFWISNLTSGLSAGLQSATQAATSGCCASA
HHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8757728; 8969508; 9384377; 1339421 [H]