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The map label for this gene is cynR [H]

Identifier: 86747267

GI number: 86747267

Start: 153001

End: 153903

Strand: Reverse

Name: cynR [H]

Synonym: RPB_0140

Alternate gene names: 86747267

Gene position: 153903-153001 (Counterclockwise)

Preceding gene: 86747281

Following gene: 86747264

Centisome position: 2.89

GC content: 67.22

Gene sequence:

>903_bases
ATGATCGAATCGGCGGCGATCCGCTATTTCCGCGAGGTGACGGAATCCGGATCGATCAAGCAGGCCGCCGCGACGCTGCG
GATCGCGCCGTCGGCGATCAGCCGGCAGATTCAGGCGATCGAGGAGGAAATGTCGGTCAAGCTGTTCGAGCGCAATGCCC
GCGGCATGGCGCTGACCGACGCCGGCCGGCTGCTGTATCGCTACGCGGTCGAGAACCGCAGCCAGCTCGACGGCATCCGC
GCCAAGGTGCAGGAATTCGAAACCATGCGCCGCGGCCAGATCCGGTTCGCCACCGTGGAAGGCATGCTGGCGAATTTCCT
GTCCAACTTCCTGGTCGAGCTGTCGGTGGATTATCCCGGCATTTTCGTCTCCACGACGGTGGTGGGGTCCCGCGCGGTCG
CCGAACTGGTCGGACGCAACGAGGTCGATCTCGGGCTGGTGTTCGGGCGGGCGCCGCGGCGCGACCTGATCGAGCTGGCG
CGGATGCGGCAGTCGCTGTGCCTGATCGTCGCGCCCGATCATCCGATGGCCGATCGCGAAGCCTGCGCGGTCAAGGATCT
CGCGGGGCTGCGGGTGATCGTCCCCGATCCGTCGTTCGGCATCCGTCAGGAAGTCGATCGCGCCTGCGCCAACGCCAAGG
TACGGCTGGAGATCTGCAGCGAGACCAATTCGCTGGCGTTCGCGCAGATGCTGGCGTCGCGCACCGATCTCGCCACCTTC
CTGCCGCGCGACACCGCGATGCCGGCGATCGCGGCGGGGCGGCTGGTGGCTGTTCCGCTGCGCGACAAGCGGTTGGAGGC
GACGCAGGTGACGCTGGTGCAACTCGCCACCCGCAATATCTCGCCGTCGTGCCGGATGGTGGCGGACCTGCTGATCGCGC
GGATGAAGCGCGCGCGGGAGTAA

Upstream 100 bases:

>100_bases
AAAGCATTGAAATTAGAGGTCTCAGATCGAATAATTAGACCATTAGCCAGCTGCTGCCGGAATCTTCGGCAGCCGGAACA
ACCCGAAGGACACGGCTGCG

Downstream 100 bases:

>100_bases
TGCTCCCCCAACCGTCATGGTCGGGCTCGTCCCGGCCATCCACGTCCTGCTTGCGGCGTGAGCCCAGTTCGTGGATGGCC
GGCACAAGGCCGGCCAAGAC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Cyn operon transcriptional activator [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MIESAAIRYFREVTESGSIKQAAATLRIAPSAISRQIQAIEEEMSVKLFERNARGMALTDAGRLLYRYAVENRSQLDGIR
AKVQEFETMRRGQIRFATVEGMLANFLSNFLVELSVDYPGIFVSTTVVGSRAVAELVGRNEVDLGLVFGRAPRRDLIELA
RMRQSLCLIVAPDHPMADREACAVKDLAGLRVIVPDPSFGIRQEVDRACANAKVRLEICSETNSLAFAQMLASRTDLATF
LPRDTAMPAIAAGRLVAVPLRDKRLEATQVTLVQLATRNISPSCRMVADLLIARMKRARE

Sequences:

>Translated_300_residues
MIESAAIRYFREVTESGSIKQAAATLRIAPSAISRQIQAIEEEMSVKLFERNARGMALTDAGRLLYRYAVENRSQLDGIR
AKVQEFETMRRGQIRFATVEGMLANFLSNFLVELSVDYPGIFVSTTVVGSRAVAELVGRNEVDLGLVFGRAPRRDLIELA
RMRQSLCLIVAPDHPMADREACAVKDLAGLRVIVPDPSFGIRQEVDRACANAKVRLEICSETNSLAFAQMLASRTDLATF
LPRDTAMPAIAAGRLVAVPLRDKRLEATQVTLVQLATRNISPSCRMVADLLIARMKRARE
>Mature_300_residues
MIESAAIRYFREVTESGSIKQAAATLRIAPSAISRQIQAIEEEMSVKLFERNARGMALTDAGRLLYRYAVENRSQLDGIR
AKVQEFETMRRGQIRFATVEGMLANFLSNFLVELSVDYPGIFVSTTVVGSRAVAELVGRNEVDLGLVFGRAPRRDLIELA
RMRQSLCLIVAPDHPMADREACAVKDLAGLRVIVPDPSFGIRQEVDRACANAKVRLEICSETNSLAFAQMLASRTDLATF
LPRDTAMPAIAAGRLVAVPLRDKRLEATQVTLVQLATRNISPSCRMVADLLIARMKRARE

Specific function: Positively regulates the cynTSX operon, and negatively regulates its own transcription. Binds specifically to the cynR- cynTSX intergenic region [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=269, Percent_Identity=29.7397769516729, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI145693105, Length=258, Percent_Identity=25.968992248062, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788297, Length=221, Percent_Identity=24.4343891402715, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787530, Length=209, Percent_Identity=24.4019138755981, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787879, Length=201, Percent_Identity=27.363184079602, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788481, Length=255, Percent_Identity=27.843137254902, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788748, Length=289, Percent_Identity=22.8373702422145, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789440, Length=113, Percent_Identity=32.7433628318584, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87082132, Length=275, Percent_Identity=23.2727272727273, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1789639, Length=115, Percent_Identity=29.5652173913043, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1790262, Length=242, Percent_Identity=25.6198347107438, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789204, Length=225, Percent_Identity=24.8888888888889, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1787601, Length=258, Percent_Identity=19.7674418604651, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33182; Mature: 33182

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIESAAIRYFREVTESGSIKQAAATLRIAPSAISRQIQAIEEEMSVKLFERNARGMALTD
CCCHHHHHHHHHHHCCCCHHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEH
AGRLLYRYAVENRSQLDGIRAKVQEFETMRRGQIRFATVEGMLANFLSNFLVELSVDYPG
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHEEEECCCCC
IFVSTTVVGSRAVAELVGRNEVDLGLVFGRAPRRDLIELARMRQSLCLIVAPDHPMADRE
EEEEHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCEEEEECCCCCCCCCH
ACAVKDLAGLRVIVPDPSFGIRQEVDRACANAKVRLEICSETNSLAFAQMLASRTDLATF
HHHHHHHCCCEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHH
LPRDTAMPAIAAGRLVAVPLRDKRLEATQVTLVQLATRNISPSCRMVADLLIARMKRARE
CCCCCCCCHHHCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIESAAIRYFREVTESGSIKQAAATLRIAPSAISRQIQAIEEEMSVKLFERNARGMALTD
CCCHHHHHHHHHHHCCCCHHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEH
AGRLLYRYAVENRSQLDGIRAKVQEFETMRRGQIRFATVEGMLANFLSNFLVELSVDYPG
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHEEEECCCCC
IFVSTTVVGSRAVAELVGRNEVDLGLVFGRAPRRDLIELARMRQSLCLIVAPDHPMADRE
EEEEHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCEEEEECCCCCCCCCH
ACAVKDLAGLRVIVPDPSFGIRQEVDRACANAKVRLEICSETNSLAFAQMLASRTDLATF
HHHHHHHCCCEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHH
LPRDTAMPAIAAGRLVAVPLRDKRLEATQVTLVQLATRNISPSCRMVADLLIARMKRARE
CCCCCCCCHHHCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]