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The map label for this gene is folD

Identifier: 86747235

GI number: 86747235

Start: 119224

End: 120111

Strand: Reverse

Name: folD

Synonym: RPB_0108

Alternate gene names: 86747235

Gene position: 120111-119224 (Counterclockwise)

Preceding gene: 86747236

Following gene: 86747229

Centisome position: 2.25

GC content: 67.45

Gene sequence:

>888_bases
ATGACTGCCACGATCATCGACGGAAAAATCATCTCGGCCGAGCTGCGCGCGCGGGTGGCCGCGGAGGTGACGCGAATCAA
GGCCGATCACGGCATCACGCCGGGGCTCGCGGTGGTGCTGGTCGGCAGCGATCCGGCCTCCGAGGTCTATGTCCGCAGCA
AGCACAAGCAGACCCAGGAGGCCGGCATGGCCTCGTTCGAGCACCGGCTGCCGGCCGATGTGCCGCAGGCCGAGCTGCTG
GCGCTGATCGGCCAGCTCAATGCCGATCCGGCGGTGCACGGAATCCTGGTGCAGTTGCCGTTGCCGAAGGGGCTGGACAG
CAATGCGGTGATCGATGCGATCGATCCTGCCAAGGACGTCGACGGCCTCAATCCGGTCAATGCCGGCCGGCTGGCCTCCG
GGCTGTTCGCGCTGACGCCGTGCACGCCGCTCGGCTGCATCATCATGGCCAAGCAGGTGCATGCCTCGCTGGAAGGCATG
AATGCCATCGTGATCGGCCGCTCCAATCTGGTCGGCAAGCCGCTGGTGCAATTGCTGCTCAACGAGAACGCCACCGTGAC
GATCGCGCATTCGCGCTCGCGCGATCTGCCGGCGCTGTGCCGGCAGGCCGATCTCGTGTTCGCCGCGGTCGGCAGGCCCG
AGATGGTGAAAGGCGACTGGATCAAGCCGGGCGCGACCGTGATCGATGTCGGCATCAACCGCACGCCGTCGCCGGACGGC
GGCAAGGACAAGCTCGTTGGTGACGTCGCCTTTGCCGAAGCCAAGGACATCGCCGGCGCCATCACCCCGGTGCCGGGCGG
CGTCGGCCTGATGACGGTGGCGTGCCTCCTGGTCAACACCGTCCGCGCCGCCAGCGCGATTCACGGCTTGCCGAAGCCGG
CCGTCTAA

Upstream 100 bases:

>100_bases
AACGAACAGGGAGGCCCCGAGGGCTGCTCCGATCGCCTCTCCGTCCGGAGAGGCTTTTTGTTTTGCGATGCGCACGCATC
ATCCCAGGCGAGGGGACGAC

Downstream 100 bases:

>100_bases
CCCATCCAGCGTCGTCATGCTGAGGTGCGCGCCGGCTTCGGCGCGCCTCGAAGCATGCGGACACGAGTGCCAGCTGCCGG
TACGCGTGCCCCATCCTTCG

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MTATIIDGKIISAELRARVAAEVTRIKADHGITPGLAVVLVGSDPASEVYVRSKHKQTQEAGMASFEHRLPADVPQAELL
ALIGQLNADPAVHGILVQLPLPKGLDSNAVIDAIDPAKDVDGLNPVNAGRLASGLFALTPCTPLGCIIMAKQVHASLEGM
NAIVIGRSNLVGKPLVQLLLNENATVTIAHSRSRDLPALCRQADLVFAAVGRPEMVKGDWIKPGATVIDVGINRTPSPDG
GKDKLVGDVAFAEAKDIAGAITPVPGGVGLMTVACLLVNTVRAASAIHGLPKPAV

Sequences:

>Translated_295_residues
MTATIIDGKIISAELRARVAAEVTRIKADHGITPGLAVVLVGSDPASEVYVRSKHKQTQEAGMASFEHRLPADVPQAELL
ALIGQLNADPAVHGILVQLPLPKGLDSNAVIDAIDPAKDVDGLNPVNAGRLASGLFALTPCTPLGCIIMAKQVHASLEGM
NAIVIGRSNLVGKPLVQLLLNENATVTIAHSRSRDLPALCRQADLVFAAVGRPEMVKGDWIKPGATVIDVGINRTPSPDG
GKDKLVGDVAFAEAKDIAGAITPVPGGVGLMTVACLLVNTVRAASAIHGLPKPAV
>Mature_294_residues
TATIIDGKIISAELRARVAAEVTRIKADHGITPGLAVVLVGSDPASEVYVRSKHKQTQEAGMASFEHRLPADVPQAELLA
LIGQLNADPAVHGILVQLPLPKGLDSNAVIDAIDPAKDVDGLNPVNAGRLASGLFALTPCTPLGCIIMAKQVHASLEGMN
AIVIGRSNLVGKPLVQLLLNENATVTIAHSRSRDLPALCRQADLVFAAVGRPEMVKGDWIKPGATVIDVGINRTPSPDGG
KDKLVGDVAFAEAKDIAGAITPVPGGVGLMTVACLLVNTVRAASAIHGLPKPAV

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=299, Percent_Identity=46.4882943143813, Blast_Score=247, Evalue=7e-66,
Organism=Homo sapiens, GI222418558, Length=295, Percent_Identity=42.3728813559322, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI94721354, Length=295, Percent_Identity=41.3559322033898, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI36796743, Length=201, Percent_Identity=28.8557213930348, Blast_Score=82, Evalue=6e-16,
Organism=Escherichia coli, GI1786741, Length=288, Percent_Identity=48.9583333333333, Blast_Score=255, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI17568735, Length=293, Percent_Identity=42.6621160409556, Blast_Score=214, Evalue=5e-56,
Organism=Saccharomyces cerevisiae, GI6319558, Length=285, Percent_Identity=45.6140350877193, Blast_Score=250, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6321643, Length=299, Percent_Identity=44.1471571906354, Blast_Score=229, Evalue=6e-61,
Organism=Saccharomyces cerevisiae, GI6322933, Length=324, Percent_Identity=25.9259259259259, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI17136818, Length=292, Percent_Identity=47.6027397260274, Blast_Score=244, Evalue=4e-65,
Organism=Drosophila melanogaster, GI17136816, Length=292, Percent_Identity=47.6027397260274, Blast_Score=244, Evalue=4e-65,
Organism=Drosophila melanogaster, GI62472483, Length=288, Percent_Identity=47.5694444444444, Blast_Score=244, Evalue=5e-65,
Organism=Drosophila melanogaster, GI45551871, Length=288, Percent_Identity=47.5694444444444, Blast_Score=244, Evalue=5e-65,
Organism=Drosophila melanogaster, GI24645718, Length=288, Percent_Identity=47.5694444444444, Blast_Score=244, Evalue=6e-65,
Organism=Drosophila melanogaster, GI17137370, Length=288, Percent_Identity=47.5694444444444, Blast_Score=244, Evalue=6e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_RHOP2 (Q2J3Z0)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_483731.1
- ProteinModelPortal:   Q2J3Z0
- SMR:   Q2J3Z0
- STRING:   Q2J3Z0
- GeneID:   3909694
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_0108
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   CTPLGCI
- ProtClustDB:   PRK14188
- BioCyc:   RPAL316058:RPB_0108-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30469; Mature: 30338

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATIIDGKIISAELRARVAAEVTRIKADHGITPGLAVVLVGSDPASEVYVRSKHKQTQE
CCEEEECCEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
AGMASFEHRLPADVPQAELLALIGQLNADPAVHGILVQLPLPKGLDSNAVIDAIDPAKDV
HHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCEEECCCCHHHC
DGLNPVNAGRLASGLFALTPCTPLGCIIMAKQVHASLEGMNAIVIGRSNLVGKPLVQLLL
CCCCCCCHHHHHCCCEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHH
NENATVTIAHSRSRDLPALCRQADLVFAAVGRPEMVKGDWIKPGATVIDVGINRTPSPDG
CCCCEEEEEECCCCCHHHHHHHHCEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCC
GKDKLVGDVAFAEAKDIAGAITPVPGGVGLMTVACLLVNTVRAASAIHGLPKPAV
CCCCEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TATIIDGKIISAELRARVAAEVTRIKADHGITPGLAVVLVGSDPASEVYVRSKHKQTQE
CEEEECCEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
AGMASFEHRLPADVPQAELLALIGQLNADPAVHGILVQLPLPKGLDSNAVIDAIDPAKDV
HHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCEEECCCCHHHC
DGLNPVNAGRLASGLFALTPCTPLGCIIMAKQVHASLEGMNAIVIGRSNLVGKPLVQLLL
CCCCCCCHHHHHCCCEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHH
NENATVTIAHSRSRDLPALCRQADLVFAAVGRPEMVKGDWIKPGATVIDVGINRTPSPDG
CCCCEEEEEECCCCCHHHHHHHHCEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCC
GKDKLVGDVAFAEAKDIAGAITPVPGGVGLMTVACLLVNTVRAASAIHGLPKPAV
CCCCEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA