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The map label for this gene is purH [H]

Identifier: 86747211

GI number: 86747211

Start: 88475

End: 90067

Strand: Reverse

Name: purH [H]

Synonym: RPB_0084

Alternate gene names: 86747211

Gene position: 90067-88475 (Counterclockwise)

Preceding gene: 86747212

Following gene: 86747209

Centisome position: 1.69

GC content: 67.04

Gene sequence:

>1593_bases
ATGACCGATCTTCCGCGCCGCGTGACCCGCGCTCTGTTGTCCGTTTCCGACAAGACCGGGCTGGTCGATTTCGCCCGCGC
GCTGGCCGGCCACGGCGTCGAACTGGTCTCGACCGGCGGCACCGCCAAGGCGATCGCGGCGGCCGGGCTGCCGGTCAAGG
ACGTCTCCGAGATCACCGGCTTTCCCGAGATGATGGACGGCCGGGTCAAGACGCTGCATCCCAAGGTGCATGGCGGCCTG
CTGGCGGTCCGCGACAATGACGAGCACAAGCAGGCGATGGCGGCGCACGGCATCGCCCAGATCGACCTCCTCGTGGTCAA
TCTGTATCCGTTCGAGGCCACCGTCGACAAAGGCGGCTCCTACGAGGACTGCATCGAGAACATCGACATCGGCGGCCCGG
CGATGATCCGCGCCGCAGCGAAGAATCACGACGACGTCGCGGTGATCGTCGAATCGTCCGACTATCAGGCGGTGCTCGAC
GAACTCGCGGCCAATGCCGGCGCCACCTCGCACGGCTTGCGCAAGCGCCTCGCCGCCAAGGCCTATGCCCGCACCGCGGC
CTACGACGCCGCGATCTCCAATTGGTTCGCGCAGCAATTGAAGACCGATGCGCCGGATTTCCGCGCGATCGGCGGCCGGC
TGATCCAGAGCCTGCGCTACGGCGAGAACCCGCATCAGACCGCGGCGTTCTACGCCACCCCGGAGAAGCGTCCGGGCGTC
GCCACCGCGCGGCAGGTGCAGGGCAAGGAACTGTCCTACAACAACATCAACGATACCGACGCGGCCTATGAATGCGTCGG
CGAGTTCGACGCCAAGCGCACTGCAGCCTGCGTCATCGTCAAGCACGCCAATCCCTGCGGCGTCGCCGAAGGATCGAGCC
TGCTCGATGCCTATCGCAAGGCGCTGGCGTGCGATTCGACCTCGGCGTTCGGCGGCATCGTCGCGCTCAACCGCACGCTC
GACGCCGAAGCCGCACGCGCGATCGTCGAGATCTTCACCGAAATGATCATCGCGCCCGAGGCGAGCGAGGAAGCGATCGC
GATCGTGGCGGCGAAGAAAAACTTGCGGCTGCTGCTGGCCGGCAGCCTGCCCAACCCGCGCGCCGCCGGCCTGACCTACA
AGAGCGTGTCCGGAGGGCTGCTGGTGCAGTCGCGCGACAATGCGGTGGTCGACGACATGGCGCTCAAGGTCGTCACCAAG
CGGCAGCCGAGCGAGGCCGAACTGCGCGACCTGAAATTCGCCTTCCGGGTCGCCAAGCACGTCAAGTCCAACACCATCAT
CTACGCCAAGGATCTGGCCACCGTCGGCATCGGCGCCGGCCAGATGAGCCGGGTCGATTCCGCCCGCATTGCCGCGCGAA
AAGCGCAGGATGCCGCCGCCGAGCTGAAACTCGCGGCGCCGATGACCAAGGGCTCGGTGGTGGCATCGGACGCGTTCTTC
CCGTTCGCCGACGGCATGCTCGCCTGCATCGAAGCCGGCGCCACCGCGGTGATCCAGCCCGGCGGCTCGGTGCGCGACGA
CGAAGTCATCAAGGCCGCGGACGACGCCGGCATCGCCATGGTGTTCACCGGGACCAGGCATTTCCGGCATTGA

Upstream 100 bases:

>100_bases
GCGAATGCCGCAGGCGGGTCCCAGACCCGCCGTTTTGGCCGGTTTCATCGCACGGAAAACCATGCTAGGCGGGCATTCTC
CCGCCCAAAGGACGCATTCC

Downstream 100 bases:

>100_bases
ACTAACCTCTCCCCGCGCGCGGGGAGAGGTCGACCGGCGAAGCGCAGCGTAGCCGGTCGGGTGAGGGGGCGTCTCCGCGC
AGCCGAGACTCAGTGCCCGC

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MTDLPRRVTRALLSVSDKTGLVDFARALAGHGVELVSTGGTAKAIAAAGLPVKDVSEITGFPEMMDGRVKTLHPKVHGGL
LAVRDNDEHKQAMAAHGIAQIDLLVVNLYPFEATVDKGGSYEDCIENIDIGGPAMIRAAAKNHDDVAVIVESSDYQAVLD
ELAANAGATSHGLRKRLAAKAYARTAAYDAAISNWFAQQLKTDAPDFRAIGGRLIQSLRYGENPHQTAAFYATPEKRPGV
ATARQVQGKELSYNNINDTDAAYECVGEFDAKRTAACVIVKHANPCGVAEGSSLLDAYRKALACDSTSAFGGIVALNRTL
DAEAARAIVEIFTEMIIAPEASEEAIAIVAAKKNLRLLLAGSLPNPRAAGLTYKSVSGGLLVQSRDNAVVDDMALKVVTK
RQPSEAELRDLKFAFRVAKHVKSNTIIYAKDLATVGIGAGQMSRVDSARIAARKAQDAAAELKLAAPMTKGSVVASDAFF
PFADGMLACIEAGATAVIQPGGSVRDDEVIKAADDAGIAMVFTGTRHFRH

Sequences:

>Translated_530_residues
MTDLPRRVTRALLSVSDKTGLVDFARALAGHGVELVSTGGTAKAIAAAGLPVKDVSEITGFPEMMDGRVKTLHPKVHGGL
LAVRDNDEHKQAMAAHGIAQIDLLVVNLYPFEATVDKGGSYEDCIENIDIGGPAMIRAAAKNHDDVAVIVESSDYQAVLD
ELAANAGATSHGLRKRLAAKAYARTAAYDAAISNWFAQQLKTDAPDFRAIGGRLIQSLRYGENPHQTAAFYATPEKRPGV
ATARQVQGKELSYNNINDTDAAYECVGEFDAKRTAACVIVKHANPCGVAEGSSLLDAYRKALACDSTSAFGGIVALNRTL
DAEAARAIVEIFTEMIIAPEASEEAIAIVAAKKNLRLLLAGSLPNPRAAGLTYKSVSGGLLVQSRDNAVVDDMALKVVTK
RQPSEAELRDLKFAFRVAKHVKSNTIIYAKDLATVGIGAGQMSRVDSARIAARKAQDAAAELKLAAPMTKGSVVASDAFF
PFADGMLACIEAGATAVIQPGGSVRDDEVIKAADDAGIAMVFTGTRHFRH
>Mature_529_residues
TDLPRRVTRALLSVSDKTGLVDFARALAGHGVELVSTGGTAKAIAAAGLPVKDVSEITGFPEMMDGRVKTLHPKVHGGLL
AVRDNDEHKQAMAAHGIAQIDLLVVNLYPFEATVDKGGSYEDCIENIDIGGPAMIRAAAKNHDDVAVIVESSDYQAVLDE
LAANAGATSHGLRKRLAAKAYARTAAYDAAISNWFAQQLKTDAPDFRAIGGRLIQSLRYGENPHQTAAFYATPEKRPGVA
TARQVQGKELSYNNINDTDAAYECVGEFDAKRTAACVIVKHANPCGVAEGSSLLDAYRKALACDSTSAFGGIVALNRTLD
AEAARAIVEIFTEMIIAPEASEEAIAIVAAKKNLRLLLAGSLPNPRAAGLTYKSVSGGLLVQSRDNAVVDDMALKVVTKR
QPSEAELRDLKFAFRVAKHVKSNTIIYAKDLATVGIGAGQMSRVDSARIAARKAQDAAAELKLAAPMTKGSVVASDAFFP
FADGMLACIEAGATAVIQPGGSVRDDEVIKAADDAGIAMVFTGTRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=471, Percent_Identity=39.9150743099788, Blast_Score=275, Evalue=1e-73,
Organism=Escherichia coli, GI1790439, Length=538, Percent_Identity=58.9219330855019, Blast_Score=583, Evalue=1e-167,
Organism=Caenorhabditis elegans, GI71985564, Length=603, Percent_Identity=32.6699834162521, Blast_Score=277, Evalue=9e-75,
Organism=Caenorhabditis elegans, GI71985574, Length=305, Percent_Identity=27.8688524590164, Blast_Score=93, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6323768, Length=472, Percent_Identity=37.5, Blast_Score=272, Evalue=9e-74,
Organism=Saccharomyces cerevisiae, GI6323056, Length=479, Percent_Identity=36.9519832985386, Blast_Score=266, Evalue=5e-72,
Organism=Drosophila melanogaster, GI24649832, Length=600, Percent_Identity=36.5, Blast_Score=303, Evalue=2e-82,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 55953; Mature: 55822

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLPRRVTRALLSVSDKTGLVDFARALAGHGVELVSTGGTAKAIAAAGLPVKDVSEITG
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHCCCCHHHHHHHCC
FPEMMDGRVKTLHPKVHGGLLAVRDNDEHKQAMAAHGIAQIDLLVVNLYPFEATVDKGGS
CHHHHCCCEEEECCHHCCCEEEEECCCHHHHHHHHCCCEEEEEEEEEEECCEEECCCCCC
YEDCIENIDIGGPAMIRAAAKNHDDVAVIVESSDYQAVLDELAANAGATSHGLRKRLAAK
HHHHHHCCCCCCHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHH
AYARTAAYDAAISNWFAQQLKTDAPDFRAIGGRLIQSLRYGENPHQTAAFYATPEKRPGV
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCC
ATARQVQGKELSYNNINDTDAAYECVGEFDAKRTAACVIVKHANPCGVAEGSSLLDAYRK
CHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHH
ALACDSTSAFGGIVALNRTLDAEAARAIVEIFTEMIIAPEASEEAIAIVAAKKNLRLLLA
HHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEEEE
GSLPNPRAAGLTYKSVSGGLLVQSRDNAVVDDMALKVVTKRQPSEAELRDLKFAFRVAKH
CCCCCCCCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
VKSNTIIYAKDLATVGIGAGQMSRVDSARIAARKAQDAAAELKLAAPMTKGSVVASDAFF
HCCCEEEEEECCCEEECCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEECCCCC
PFADGMLACIEAGATAVIQPGGSVRDDEVIKAADDAGIAMVFTGTRHFRH
CHHHHHHHHHHCCCEEEECCCCCCCCCHHHEECCCCCEEEEEECCHHCCC
>Mature Secondary Structure 
TDLPRRVTRALLSVSDKTGLVDFARALAGHGVELVSTGGTAKAIAAAGLPVKDVSEITG
CCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHCCCCHHHHHHHCC
FPEMMDGRVKTLHPKVHGGLLAVRDNDEHKQAMAAHGIAQIDLLVVNLYPFEATVDKGGS
CHHHHCCCEEEECCHHCCCEEEEECCCHHHHHHHHCCCEEEEEEEEEEECCEEECCCCCC
YEDCIENIDIGGPAMIRAAAKNHDDVAVIVESSDYQAVLDELAANAGATSHGLRKRLAAK
HHHHHHCCCCCCHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHH
AYARTAAYDAAISNWFAQQLKTDAPDFRAIGGRLIQSLRYGENPHQTAAFYATPEKRPGV
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCC
ATARQVQGKELSYNNINDTDAAYECVGEFDAKRTAACVIVKHANPCGVAEGSSLLDAYRK
CHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHH
ALACDSTSAFGGIVALNRTLDAEAARAIVEIFTEMIIAPEASEEAIAIVAAKKNLRLLLA
HHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEEEE
GSLPNPRAAGLTYKSVSGGLLVQSRDNAVVDDMALKVVTKRQPSEAELRDLKFAFRVAKH
CCCCCCCCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
VKSNTIIYAKDLATVGIGAGQMSRVDSARIAARKAQDAAAELKLAAPMTKGSVVASDAFF
HCCCEEEEEECCCEEECCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEECCCCC
PFADGMLACIEAGATAVIQPGGSVRDDEVIKAADDAGIAMVFTGTRHFRH
CHHHHHHHHHHCCCEEEECCCCCCCCCHHHEECCCCCEEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]