Click here to switch to the map view.

The map label for this gene is infC [H]

Identifier: 86747192

GI number: 86747192

Start: 68769

End: 69206

Strand: Reverse

Name: infC [H]

Synonym: RPB_0065

Alternate gene names: 86747192

Gene position: 69206-68769 (Counterclockwise)

Preceding gene: 86747195

Following gene: 86747187

Centisome position: 1.3

GC content: 58.45

Gene sequence:

>438_bases
GTGAACCAGGGCAAGGTCGAGACCATCGTCGCGATCAAGATGGCGATGGAAGCCGGCATGGATCTGGTCGAGATTTCGCC
GAACGTCAGTCCGCCGGTCTGCAAGATCATGGACTACGGCAAGTTCAAATACTCGGCGCAGAAGAAGGCCGCCGAGGCGC
GCAAAAAGCAGAAGATCGTCGAGATCAAGGAGATCAAGCTCCGCCCGATGATCGACGATCACGACTACGACGTGAAGATG
AAGGCGATGCAGCGCTTCTTCGAGGAAGGCGACAAGGTGAAGATCACTTTGCGCTATCGCGGACGCGAAATGGCCCACCA
GGAAATCGGCACCAAGTTGCTTGAAAAGGTGAAGGCCGACGTCGCCGAAATCGCCAAGGTGGAGCAGGACGCCCGCTTCG
AAGGCCGTCAGGTGGTCATGGTGCTGGCGCCGCGCTGA

Upstream 100 bases:

>100_bases
TCGCCGTCCCAACAGAGCCCCGCCCCCCGTTGCCAAGGATGGGCCGCGCACCAACGATGAAATTCGCAATCACGAGATCC
AGCTGATCGACCAGGCCGGT

Downstream 100 bases:

>100_bases
GGCGCGACAAGCCGATTTGCATTTGAGTTCGCGTGTGGCCCGTCAGTCGATCTGGCGGGCCTTTTTCTTGATGCGAGGGT
GATGACGCGCTTTGGCGTTG

Product: translation initiation factor IF-3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 145; Mature: 145

Protein sequence:

>145_residues
MNQGKVETIVAIKMAMEAGMDLVEISPNVSPPVCKIMDYGKFKYSAQKKAAEARKKQKIVEIKEIKLRPMIDDHDYDVKM
KAMQRFFEEGDKVKITLRYRGREMAHQEIGTKLLEKVKADVAEIAKVEQDARFEGRQVVMVLAPR

Sequences:

>Translated_145_residues
MNQGKVETIVAIKMAMEAGMDLVEISPNVSPPVCKIMDYGKFKYSAQKKAAEARKKQKIVEIKEIKLRPMIDDHDYDVKM
KAMQRFFEEGDKVKITLRYRGREMAHQEIGTKLLEKVKADVAEIAKVEQDARFEGRQVVMVLAPR
>Mature_145_residues
MNQGKVETIVAIKMAMEAGMDLVEISPNVSPPVCKIMDYGKFKYSAQKKAAEARKKQKIVEIKEIKLRPMIDDHDYDVKM
KAMQRFFEEGDKVKITLRYRGREMAHQEIGTKLLEKVKADVAEIAKVEQDARFEGRQVVMVLAPR

Specific function: IF-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins [H]

COG id: COG0290

COG function: function code J; Translation initiation factor 3 (IF-3)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-3 family [H]

Homologues:

Organism=Escherichia coli, GI1788012, Length=136, Percent_Identity=59.5588235294118, Blast_Score=159, Evalue=6e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001288
- InterPro:   IPR019815
- InterPro:   IPR019814 [H]

Pfam domain/function: PF00707 IF3_C; PF05198 IF3_N [H]

EC number: NA

Molecular weight: Translated: 16643; Mature: 16643

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
6.9 %Met     (Translated Protein)
7.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
6.9 %Met     (Mature Protein)
7.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQGKVETIVAIKMAMEAGMDLVEISPNVSPPVCKIMDYGKFKYSAQKKAAEARKKQKIV
CCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHH
EIKEIKLRPMIDDHDYDVKMKAMQRFFEEGDKVKITLRYRGREMAHQEIGTKLLEKVKAD
HHHHEEECCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHH
VAEIAKVEQDARFEGRQVVMVLAPR
HHHHHHHHHCCCCCCCEEEEEEECC
>Mature Secondary Structure
MNQGKVETIVAIKMAMEAGMDLVEISPNVSPPVCKIMDYGKFKYSAQKKAAEARKKQKIV
CCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHH
EIKEIKLRPMIDDHDYDVKMKAMQRFFEEGDKVKITLRYRGREMAHQEIGTKLLEKVKAD
HHHHEEECCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHH
VAEIAKVEQDARFEGRQVVMVLAPR
HHHHHHHHHCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]