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The map label for this gene is yafV [C]

Identifier: 86747151

GI number: 86747151

Start: 23874

End: 24752

Strand: Reverse

Name: yafV [C]

Synonym: RPB_0024

Alternate gene names: 86747151

Gene position: 24752-23874 (Counterclockwise)

Preceding gene: 86747152

Following gene: 86747150

Centisome position: 0.46

GC content: 66.89

Gene sequence:

>879_bases
ATGACGGAAACCGCGCCCTTCAATGCCGCGCTGGTGCAGATGCGCACCGGGCTCCTGCCCGGGCCGAGCCTCGAGCAGGG
GACCAAGCTGATCCGCGAGGCGGCGGCGGCGGGCGCCGACTACGTGCTCACCCCCGAGGTGAGCAACATGATGCAGCTCA
ACCGCGAGGCTTTGTTCGACCAACTCGCCGACGAGGACAGCGACGTATCGCTGAAGGCCTATCGCGATCTCGCGCGCGAG
CTGAAAATTCATCTGCATATCGGCTCGCTGGCGCTGCGCGCGAGCCCGGATCGGGCCGTCAACCGCTCGTTCCTGATCGA
TCCGTCGGGGACGATTCTGGCGAGCTACGACAAGATCCATATGTTCGACATCGATCTCGGCAATGGCGAGAGCTATCGCG
AATCGGCGAACTACCAGCCCGGCGAAACCGCGGTGATCTCCGATCTGCCGTGGGGCCGGATCGGGCTGACGATCTGCTAC
GATGTGCGCTTCCCGGCGCTGTATCGCGCGCTCGCCGAAGCCGGCGCGTCGTTCATCTCCGTGCCGGCGGCGTTCACCCG
GCCGACCGGCGAGGCGCATTGGCACGTGCTGCTGCGGGCGCGGGCCATCGAAAACGGCTGCTTCGTGTTCGCCGCCGCGC
AAGGCGGGCTGCACGAGAACAAGCGCGAGACCTACGGCCATTCGCTGATCATCGATCCGTGGGGCGTGGTGCTGGCCGAA
GGCGGCGTCGATCCGGGCTTCGTGATGGCGCGGATCGATCCGGCCGAGGTCGGCAAGGCGCGCGGCAAGATCCCGTCGCT
GCAGCACGGCCGCCGCTTCACGCTGGTCGACGCCAAAGCGACGCCCGACTATCTGCATCTCGTGCGGGAGCAGACATGA

Upstream 100 bases:

>100_bases
TCTTCATCGGCGGCACCCATGTCGGCGGCTGCGACGACCTCTATGCGCTCGATCGCGAAGGCAAGCTGGACGCGCTCTTG
GCCGGCGAGAAGGCCACGTC

Downstream 100 bases:

>100_bases
TCCGCTACACGCTGCGGTGCGATCGTGGCCACTCGTTCGAGAGCTGGTTCCAGAGCTCGTCGGCTTACGATTCGCAGGTG
CGGCGCAAGCTGGTGGAGTG

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MTETAPFNAALVQMRTGLLPGPSLEQGTKLIREAAAAGADYVLTPEVSNMMQLNREALFDQLADEDSDVSLKAYRDLARE
LKIHLHIGSLALRASPDRAVNRSFLIDPSGTILASYDKIHMFDIDLGNGESYRESANYQPGETAVISDLPWGRIGLTICY
DVRFPALYRALAEAGASFISVPAAFTRPTGEAHWHVLLRARAIENGCFVFAAAQGGLHENKRETYGHSLIIDPWGVVLAE
GGVDPGFVMARIDPAEVGKARGKIPSLQHGRRFTLVDAKATPDYLHLVREQT

Sequences:

>Translated_292_residues
MTETAPFNAALVQMRTGLLPGPSLEQGTKLIREAAAAGADYVLTPEVSNMMQLNREALFDQLADEDSDVSLKAYRDLARE
LKIHLHIGSLALRASPDRAVNRSFLIDPSGTILASYDKIHMFDIDLGNGESYRESANYQPGETAVISDLPWGRIGLTICY
DVRFPALYRALAEAGASFISVPAAFTRPTGEAHWHVLLRARAIENGCFVFAAAQGGLHENKRETYGHSLIIDPWGVVLAE
GGVDPGFVMARIDPAEVGKARGKIPSLQHGRRFTLVDAKATPDYLHLVREQT
>Mature_291_residues
TETAPFNAALVQMRTGLLPGPSLEQGTKLIREAAAAGADYVLTPEVSNMMQLNREALFDQLADEDSDVSLKAYRDLAREL
KIHLHIGSLALRASPDRAVNRSFLIDPSGTILASYDKIHMFDIDLGNGESYRESANYQPGETAVISDLPWGRIGLTICYD
VRFPALYRALAEAGASFISVPAAFTRPTGEAHWHVLLRARAIENGCFVFAAAQGGLHENKRETYGHSLIIDPWGVVLAEG
GVDPGFVMARIDPAEVGKARGKIPSLQHGRRFTLVDAKATPDYLHLVREQT

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI297632348, Length=250, Percent_Identity=39.6, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI297632350, Length=250, Percent_Identity=39.6, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI5031947, Length=250, Percent_Identity=39.6, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI9910460, Length=265, Percent_Identity=33.2075471698113, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI297632346, Length=173, Percent_Identity=34.1040462427746, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1786412, Length=189, Percent_Identity=31.2169312169312, Blast_Score=74, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17556280, Length=265, Percent_Identity=37.7358490566038, Blast_Score=177, Evalue=8e-45,
Organism=Saccharomyces cerevisiae, GI6322335, Length=285, Percent_Identity=33.6842105263158, Blast_Score=161, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6323383, Length=210, Percent_Identity=34.2857142857143, Blast_Score=143, Evalue=3e-35,
Organism=Drosophila melanogaster, GI17933642, Length=268, Percent_Identity=36.5671641791045, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI21355835, Length=196, Percent_Identity=32.6530612244898, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 31897; Mature: 31765

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETAPFNAALVQMRTGLLPGPSLEQGTKLIREAAAAGADYVLTPEVSNMMQLNREALFD
CCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHH
QLADEDSDVSLKAYRDLARELKIHLHIGSLALRASPDRAVNRSFLIDPSGTILASYDKIH
HHCCCCCCCHHHHHHHHHHHEEEEEEEEEEEEECCCCCCCCCEEEECCCCCEEEECCEEE
MFDIDLGNGESYRESANYQPGETAVISDLPWGRIGLTICYDVRFPALYRALAEAGASFIS
EEEEECCCCCCHHHCCCCCCCCEEEEECCCCCCEEEEEEEECCCHHHHHHHHHCCCCEEE
VPAAFTRPTGEAHWHVLLRARAIENGCFVFAAAQGGLHENKRETYGHSLIIDPWGVVLAE
CCCHHCCCCCCCEEEEEEEEEECCCCEEEEEECCCCCCCCCHHHCCCEEEECCCEEEEEC
GGVDPGFVMARIDPAEVGKARGKIPSLQHGRRFTLVDAKATPDYLHLVREQT
CCCCCCEEEEEECHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHCCC
>Mature Secondary Structure 
TETAPFNAALVQMRTGLLPGPSLEQGTKLIREAAAAGADYVLTPEVSNMMQLNREALFD
CCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHH
QLADEDSDVSLKAYRDLARELKIHLHIGSLALRASPDRAVNRSFLIDPSGTILASYDKIH
HHCCCCCCCHHHHHHHHHHHEEEEEEEEEEEEECCCCCCCCCEEEECCCCCEEEECCEEE
MFDIDLGNGESYRESANYQPGETAVISDLPWGRIGLTICYDVRFPALYRALAEAGASFIS
EEEEECCCCCCHHHCCCCCCCCEEEEECCCCCCEEEEEEEECCCHHHHHHHHHCCCCEEE
VPAAFTRPTGEAHWHVLLRARAIENGCFVFAAAQGGLHENKRETYGHSLIIDPWGVVLAE
CCCHHCCCCCCCEEEEEEEEEECCCCEEEEEECCCCCCCCCHHHCCCEEEECCCEEEEEC
GGVDPGFVMARIDPAEVGKARGKIPSLQHGRRFTLVDAKATPDYLHLVREQT
CCCCCCEEEEEECHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]