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The map label for this gene is budC [H]

Identifier: 86747146

GI number: 86747146

Start: 19785

End: 20522

Strand: Reverse

Name: budC [H]

Synonym: RPB_0019

Alternate gene names: 86747146

Gene position: 20522-19785 (Counterclockwise)

Preceding gene: 86747147

Following gene: 86747145

Centisome position: 0.38

GC content: 68.29

Gene sequence:

>738_bases
ATGACGCCACGCAACGCAACCGTCGCGGTGATCGGCGCCGGCGACTACATCGGCGCCGAGATCGCCAGGAAATTCGCCGC
CGAAGGCTTCACGGTGTTCGCCGGCCGGCGCGACGGCGCCAAGTTGGAGCCGCTGGTGCGGGAGATCGAGGCGGCCGGCG
GCCGCATCGTCGCGCGCACGCTGGATGCCCGCAAGGAGGACGAGGTCGCGGCGTTTCTCGACGCCGCCGACGCGCATGCG
CCGCTCGACGTGACGATCTTCAACGTCGGCGCCAACGTCAATTTCCCGATTCTGGAGACCACCGACCGGGTGTTCCGCAA
GGTCTGGGAAATGGCGTGCTGGGCGGGCTTTCTCGCCGGGCGGGAATCGGCGCGGCTGATGCTGCCGCGCGGACGGGGCA
ACATTTTCTTCACCGGCGCGACCGCGTCGCTCCGCGGCGGCTCGGGTTTCGCGGCCTTCGCCAGCGCCAAATTCGGGCTG
CGCGCGGTGGCGCAGTCGATGGCGCGCGAATTGATGCCGCAGAACATCCACGTCGCGCATCTGATCATCGATTCGGGGGT
CGACACCGCCTGGGTCCGCGAGCGCCGCGAGCAGCTCTGGGGCAAGCAGGCGCTCGACAATCCCGACCTCCTGATGCCGC
CGGCCTCGGTCGCCGGGGCCTATTGGCAGCTCTATCAGCAGCCGAAAAGCGCCTGGACCTTCGAGATGGAAATCCGTCCC
TTCGGGGAAAAGTGGTAG

Upstream 100 bases:

>100_bases
CCATCCACGGACACCGGCGCAGCGGTTGCGTCATCGTGGATGCCCGGGACGAGCCCGGGCATGACGAACTCATAACCCGG
TTCAATTCGGAGAAGCCAAC

Downstream 100 bases:

>100_bases
GGGCCATGCAAAGCCGGCGCTCGCCATGGCGGGGCCGTCGCGATAGGAGTTCGCAACATTCAAGGGAGGGGTGAGCGATG
CGGATTCTGATTGTCGGCGC

Product: short-chain dehydrogenase/reductase SDR

Products: NA

Alternate protein names: Acetoin(diacetyl) reductase; AR; Meso-2,3-butanediol dehydrogenase [H]

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MTPRNATVAVIGAGDYIGAEIARKFAAEGFTVFAGRRDGAKLEPLVREIEAAGGRIVARTLDARKEDEVAAFLDAADAHA
PLDVTIFNVGANVNFPILETTDRVFRKVWEMACWAGFLAGRESARLMLPRGRGNIFFTGATASLRGGSGFAAFASAKFGL
RAVAQSMARELMPQNIHVAHLIIDSGVDTAWVRERREQLWGKQALDNPDLLMPPASVAGAYWQLYQQPKSAWTFEMEIRP
FGEKW

Sequences:

>Translated_245_residues
MTPRNATVAVIGAGDYIGAEIARKFAAEGFTVFAGRRDGAKLEPLVREIEAAGGRIVARTLDARKEDEVAAFLDAADAHA
PLDVTIFNVGANVNFPILETTDRVFRKVWEMACWAGFLAGRESARLMLPRGRGNIFFTGATASLRGGSGFAAFASAKFGL
RAVAQSMARELMPQNIHVAHLIIDSGVDTAWVRERREQLWGKQALDNPDLLMPPASVAGAYWQLYQQPKSAWTFEMEIRP
FGEKW
>Mature_244_residues
TPRNATVAVIGAGDYIGAEIARKFAAEGFTVFAGRRDGAKLEPLVREIEAAGGRIVARTLDARKEDEVAAFLDAADAHAP
LDVTIFNVGANVNFPILETTDRVFRKVWEMACWAGFLAGRESARLMLPRGRGNIFFTGATASLRGGSGFAAFASAKFGLR
AVAQSMARELMPQNIHVAHLIIDSGVDTAWVRERREQLWGKQALDNPDLLMPPASVAGAYWQLYQQPKSAWTFEMEIRPF
GEKW

Specific function: Catalyzes the irreversible reduction of 2,3-butanediol to (S)-acetoin in the presence of NADH [H]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322067, Length=197, Percent_Identity=26.9035532994924, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014007
- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.1.1.304 [H]

Molecular weight: Translated: 26856; Mature: 26725

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPRNATVAVIGAGDYIGAEIARKFAAEGFTVFAGRRDGAKLEPLVREIEAAGGRIVART
CCCCCCEEEEEECCCHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHCCCEEEEEE
LDARKEDEVAAFLDAADAHAPLDVTIFNVGANVNFPILETTDRVFRKVWEMACWAGFLAG
CCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCEECCHHHHHHHHHHHHHHHHHHCC
RESARLMLPRGRGNIFFTGATASLRGGSGFAAFASAKFGLRAVAQSMARELMPQNIHVAH
CCCCEEEEECCCCCEEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
LIIDSGVDTAWVRERREQLWGKQALDNPDLLMPPASVAGAYWQLYQQPKSAWTFEMEIRP
EEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECC
FGEKW
CCCCC
>Mature Secondary Structure 
TPRNATVAVIGAGDYIGAEIARKFAAEGFTVFAGRRDGAKLEPLVREIEAAGGRIVART
CCCCCEEEEEECCCHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHCCCEEEEEE
LDARKEDEVAAFLDAADAHAPLDVTIFNVGANVNFPILETTDRVFRKVWEMACWAGFLAG
CCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCEECCHHHHHHHHHHHHHHHHHHCC
RESARLMLPRGRGNIFFTGATASLRGGSGFAAFASAKFGLRAVAQSMARELMPQNIHVAH
CCCCEEEEECCCCCEEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
LIIDSGVDTAWVRERREQLWGKQALDNPDLLMPPASVAGAYWQLYQQPKSAWTFEMEIRP
EEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECC
FGEKW
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8444801 [H]