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The map label for this gene is gltX [H]

Identifier: 86609140

GI number: 86609140

Start: 1756309

End: 1757748

Strand: Reverse

Name: gltX [H]

Synonym: CYB_1680

Alternate gene names: 86609140

Gene position: 1757748-1756309 (Counterclockwise)

Preceding gene: 86609141

Following gene: 86609139

Centisome position: 57.69

GC content: 60.35

Gene sequence:

>1440_bases
GTGAGTGTTTGTGTTCGCATTGCCCCCAGCCCAACCGGAAATCTGCACATCGGCACTGCCCGAACGGCAGTGTTCAACTG
GCTGTATGCCCGTCGCCACGGTGGCCGGTTCATTTTGCGCATCGAGGACACCGACCGCGACCGCTCTTTGCCCCGCTACA
CCCGCAACATTTTGGCGGGCCTGGCCTGGTTGGGCCTAGACTGGGACGAAGGGCCGGTCTACCAATCCAAGCGCATCGAG
CGCTATCAAGCGGTGGTACAGCAGCTTCTGGATCGAGGGTTGGCCTACCGCTGCTACGTCAGCGAGGCGGAGCTGGAGGA
GATGCGCGCTGCCCAGAAGGCCGCCGGCAAAGCCCCCCGCTACGACAACCGCCACCGCTTTCTCACCGAAGCCCAGCGAC
GGGCCTACGAAGCCGAGGGTCGGCAGCCGGTGATCCGCTTCAAAATCGAGGAGCCCCTGGAAGTGTCTTGGGTGGATCTC
ATTCGCGGCCCCATTACCTGGAACACCCAGGACTTGGGGGGGGATATGGTCATTGCCCGGGCGGATGGCTGCCCTCTCTA
CAACTTGGCGGTGGTGGTGGATGACATCGACATGGGCATCACCCATGTGATTCGCGGCGAAGATCACATCGGCAATACCC
CCAAGCAGATTTTGCTCTACCGTGCTCTTGGCCACGAACCTCCCCAGTTTGCCCATTCTCCGCTAATCCTGAACCCGGAG
GGGAAAAAGCTCTCCAAACGGGATGGGGCAACTTCGGTAGCAGAGTTCCAGCAACTGGGCTTTTTGCCGGAAGCTCTCAA
GAACTATTTGGCTTTGCTCAGTTGGTCGCCCCCGGATGGGGAAGAGCTTTTCTCTTTGGAAAAGGCGGCTGCGCTGTTCG
ACTTTGACCGGGTTAACCGTGCGGCTGCCCGCTTTGACTGGGACAAGCTCAACTGGATCAACAGCCAGTACATCAAGCGG
CTTTCTCCGCCAGAACTGGTAGAACGGCTCACCCCCTTTTGGCAGGCGGCAGGGTTTGACCTTTCCGAGGTGCCGGATCC
CACCTGGTTGGAAGATGTGGCCCGCCTGATTGCCGATGGTATCGACCGCCTGGCAGAAGCTCCTCCGCTCAGCCGCTTTT
TGTTTCAGGAGCCCCTCAGCTACACGTTGCCAGCTCTGGAACAGTTGCGCCTGCCTGGGGTGGCAGAGGCTATGGCTGCA
ATGGCCACCACGTTGGCTGCTGCCGAGCTGCCCCAGGTTTCGGCGGACAGCTTAAAACCGCTGGTGGATCAGGTGGCCAA
GGGCCAGAACATGAAAAAGGGCCTGCTGATGAAGAGTTTGCGGGCCGCCCTGACAGGGGATCTGCAGGGGCCGGATTTGC
TGGAATCTTTTGCCCTTCTCCAGCGGCGGGGCTGGGCTTTGGGGCGGTTAGAGGCTGTGCAGAAGTTGGTTCCTTGTTGA

Upstream 100 bases:

>100_bases
CCTCAATTGGCAACCGCCTGCAACCCAATGGAGGGCCAATATGACAAAGGATCTCGCCGCCGACTAGGATCAGGATGCTC
TGCTTGGGATCCTTAGACCT

Downstream 100 bases:

>100_bases
TGGAAGCCCATCTCTTGCTGTCATGCCTTCGGTTGCCTCTGTTGAAAGTGCTCCGCACCGCTGCCGCAATCGGGATCCCA
TGCTGTCACGCCAGCTACAG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MSVCVRIAPSPTGNLHIGTARTAVFNWLYARRHGGRFILRIEDTDRDRSLPRYTRNILAGLAWLGLDWDEGPVYQSKRIE
RYQAVVQQLLDRGLAYRCYVSEAELEEMRAAQKAAGKAPRYDNRHRFLTEAQRRAYEAEGRQPVIRFKIEEPLEVSWVDL
IRGPITWNTQDLGGDMVIARADGCPLYNLAVVVDDIDMGITHVIRGEDHIGNTPKQILLYRALGHEPPQFAHSPLILNPE
GKKLSKRDGATSVAEFQQLGFLPEALKNYLALLSWSPPDGEELFSLEKAAALFDFDRVNRAAARFDWDKLNWINSQYIKR
LSPPELVERLTPFWQAAGFDLSEVPDPTWLEDVARLIADGIDRLAEAPPLSRFLFQEPLSYTLPALEQLRLPGVAEAMAA
MATTLAAAELPQVSADSLKPLVDQVAKGQNMKKGLLMKSLRAALTGDLQGPDLLESFALLQRRGWALGRLEAVQKLVPC

Sequences:

>Translated_479_residues
MSVCVRIAPSPTGNLHIGTARTAVFNWLYARRHGGRFILRIEDTDRDRSLPRYTRNILAGLAWLGLDWDEGPVYQSKRIE
RYQAVVQQLLDRGLAYRCYVSEAELEEMRAAQKAAGKAPRYDNRHRFLTEAQRRAYEAEGRQPVIRFKIEEPLEVSWVDL
IRGPITWNTQDLGGDMVIARADGCPLYNLAVVVDDIDMGITHVIRGEDHIGNTPKQILLYRALGHEPPQFAHSPLILNPE
GKKLSKRDGATSVAEFQQLGFLPEALKNYLALLSWSPPDGEELFSLEKAAALFDFDRVNRAAARFDWDKLNWINSQYIKR
LSPPELVERLTPFWQAAGFDLSEVPDPTWLEDVARLIADGIDRLAEAPPLSRFLFQEPLSYTLPALEQLRLPGVAEAMAA
MATTLAAAELPQVSADSLKPLVDQVAKGQNMKKGLLMKSLRAALTGDLQGPDLLESFALLQRRGWALGRLEAVQKLVPC
>Mature_478_residues
SVCVRIAPSPTGNLHIGTARTAVFNWLYARRHGGRFILRIEDTDRDRSLPRYTRNILAGLAWLGLDWDEGPVYQSKRIER
YQAVVQQLLDRGLAYRCYVSEAELEEMRAAQKAAGKAPRYDNRHRFLTEAQRRAYEAEGRQPVIRFKIEEPLEVSWVDLI
RGPITWNTQDLGGDMVIARADGCPLYNLAVVVDDIDMGITHVIRGEDHIGNTPKQILLYRALGHEPPQFAHSPLILNPEG
KKLSKRDGATSVAEFQQLGFLPEALKNYLALLSWSPPDGEELFSLEKAAALFDFDRVNRAAARFDWDKLNWINSQYIKRL
SPPELVERLTPFWQAAGFDLSEVPDPTWLEDVARLIADGIDRLAEAPPLSRFLFQEPLSYTLPALEQLRLPGVAEAMAAM
ATTLAAAELPQVSADSLKPLVDQVAKGQNMKKGLLMKSLRAALTGDLQGPDLLESFALLQRRGWALGRLEAVQKLVPC

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=479, Percent_Identity=35.6993736951983, Blast_Score=253, Evalue=4e-67,
Organism=Escherichia coli, GI1788743, Length=453, Percent_Identity=41.280353200883, Blast_Score=344, Evalue=6e-96,
Organism=Escherichia coli, GI226510925, Length=248, Percent_Identity=35.8870967741936, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1786895, Length=207, Percent_Identity=26.5700483091787, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71993394, Length=474, Percent_Identity=32.7004219409283, Blast_Score=215, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6324540, Length=466, Percent_Identity=32.618025751073, Blast_Score=235, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6321192, Length=207, Percent_Identity=26.0869565217391, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6324742, Length=126, Percent_Identity=30.1587301587302, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24665321, Length=487, Percent_Identity=35.523613963039, Blast_Score=270, Evalue=1e-72,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 53767; Mature: 53636

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVCVRIAPSPTGNLHIGTARTAVFNWLYARRHGGRFILRIEDTDRDRSLPRYTRNILAG
CCEEEEECCCCCCCEEECHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHH
LAWLGLDWDEGPVYQSKRIERYQAVVQQLLDRGLAYRCYVSEAELEEMRAAQKAAGKAPR
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCCCCC
YDNRHRFLTEAQRRAYEAEGRQPVIRFKIEEPLEVSWVDLIRGPITWNTQDLGGDMVIAR
CCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHCCCCCCCCCCCCCCEEEEE
ADGCPLYNLAVVVDDIDMGITHVIRGEDHIGNTPKQILLYRALGHEPPQFAHSPLILNPE
CCCCCEEEEEEEEECCCCCHHHEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEECCC
GKKLSKRDGATSVAEFQQLGFLPEALKNYLALLSWSPPDGEELFSLEKAAALFDFDRVNR
CCCCHHCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
AAARFDWDKLNWINSQYIKRLSPPELVERLTPFWQAAGFDLSEVPDPTWLEDVARLIADG
HHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHH
IDRLAEAPPLSRFLFQEPLSYTLPALEQLRLPGVAEAMAAMATTLAAAELPQVSADSLKP
HHHHHCCCCHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
LVDQVAKGQNMKKGLLMKSLRAALTGDLQGPDLLESFALLQRRGWALGRLEAVQKLVPC
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SVCVRIAPSPTGNLHIGTARTAVFNWLYARRHGGRFILRIEDTDRDRSLPRYTRNILAG
CEEEEECCCCCCCEEECHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHH
LAWLGLDWDEGPVYQSKRIERYQAVVQQLLDRGLAYRCYVSEAELEEMRAAQKAAGKAPR
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCCCCC
YDNRHRFLTEAQRRAYEAEGRQPVIRFKIEEPLEVSWVDLIRGPITWNTQDLGGDMVIAR
CCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHCCCCCCCCCCCCCCEEEEE
ADGCPLYNLAVVVDDIDMGITHVIRGEDHIGNTPKQILLYRALGHEPPQFAHSPLILNPE
CCCCCEEEEEEEEECCCCCHHHEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEECCC
GKKLSKRDGATSVAEFQQLGFLPEALKNYLALLSWSPPDGEELFSLEKAAALFDFDRVNR
CCCCHHCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
AAARFDWDKLNWINSQYIKRLSPPELVERLTPFWQAAGFDLSEVPDPTWLEDVARLIADG
HHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHH
IDRLAEAPPLSRFLFQEPLSYTLPALEQLRLPGVAEAMAAMATTLAAAELPQVSADSLKP
HHHHHCCCCHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
LVDQVAKGQNMKKGLLMKSLRAALTGDLQGPDLLESFALLQRRGWALGRLEAVQKLVPC
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA