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The map label for this gene is dus1 [H]

Identifier: 86609094

GI number: 86609094

Start: 1702789

End: 1703874

Strand: Reverse

Name: dus1 [H]

Synonym: CYB_1632

Alternate gene names: 86609094

Gene position: 1703874-1702789 (Counterclockwise)

Preceding gene: 86609095

Following gene: 86609090

Centisome position: 55.93

GC content: 60.04

Gene sequence:

>1086_bases
ATGGGCCTGCAGCTTTCGCCGGAACTCCAAGCCCGCTTGTCCCAGCCCCTCAATATCGGCGGGGTAAAAGTTTTGAGCCG
CGTTTTTCAGGCGCCACTGTCAGGGGTAACCGACCAGGTCTTTCGAGGGCTGGTACGCCGTTTTGCCCCCCACAGCTTGG
TCTACACGGAAATGGTCAGTGCGACCGGCTTGCACTATGCCCGCCAGTTGCCCCGCATCATGGAGGTCGGCGAGGGAGAG
CAGCCTATTGGCATCCAACTGTTTGATTGTCGGCCCGACTTTCTGGCGGAAGCAGCCCGCATGGCTGTGGACGAAGGGGC
GACCTTGGTGGACATCAACATGGGCTGCCCGGTGAACAAGATTACCAAAAACGGCGGCGGCTCTTCTCTGCTTCGCGATC
CCGACACGGCAGCGCGAATTGTGGAGGCGGTGGCCAACGCAGTGGAGGTGCCCGTAACCGTGAAAACGCGCCTGGGCTGG
TCGGATCAGGAGATCAACATTTTGGATTTTGCCCACCGTCTGCAGGAGGCAGGGGCGCAACTGCTCACCCTGCATGCCCG
GACACGCGCCCAAGGCTTCAACGGCAAAGCCCGCTGGGACTGGATCCGCCGCGTCAAACAGCACCTGCGCATTCCCGTCA
TCGCCAATGGGGACATTTTCTCGACAGCCGCAGCGGTGAAGTGTCTGGAGGAAACCGGAGCCGATGGGGTGATGTGCTCC
CGTGGCACCATGGGCTATCCCTTTCTGGTGGGGGAAATCGATCACTTCCTGAAAACCGGTCAAGAAAGACCGGCCCCCAC
TCCCCTGGAGCGGATTCAAATGGCCTTAGAGCATCTGCGCGGTCTCTGGGAGTACAAGGGAGAACGTGGCATCCGTCAGG
CCCGCAAGCACATGACTTGGTACATCAAAGACTTTGCCGGAGCCTCTCAATTCCGTGCTCTCCTCTGTCGCATTGAGACG
GTGCAGGAAGGGGAAGCCCTGCTTCTTCAAGCCATGGAGCGCTGCCAAGAAGCCACACTTCGACCGGAAGAGGCCATTAA
AACCACCGAGGAGGTCGACCTTCTCCCTGAAGCCGGTTGGGCGTAG

Upstream 100 bases:

>100_bases
GCCAAACAGCAGCGGGAGCTCACTGCCGCGATCAAAAGAGCGCGCATCATGGGTCTTCTTTACTTTGTCAACAAAGAGGG
ATAGCTTTCTCCGGATCCCG

Downstream 100 bases:

>100_bases
CTCCAAGGATCCCCACAATTGGAAATTGCTTGGAAATCGCCGCACGTCTGCCTTGGCTGTAGCCTGTGGCCCCGAAAAAG
GCTATTGCGCCTGCTGCTGG

Product: dihydrouridine synthase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MGLQLSPELQARLSQPLNIGGVKVLSRVFQAPLSGVTDQVFRGLVRRFAPHSLVYTEMVSATGLHYARQLPRIMEVGEGE
QPIGIQLFDCRPDFLAEAARMAVDEGATLVDINMGCPVNKITKNGGGSSLLRDPDTAARIVEAVANAVEVPVTVKTRLGW
SDQEINILDFAHRLQEAGAQLLTLHARTRAQGFNGKARWDWIRRVKQHLRIPVIANGDIFSTAAAVKCLEETGADGVMCS
RGTMGYPFLVGEIDHFLKTGQERPAPTPLERIQMALEHLRGLWEYKGERGIRQARKHMTWYIKDFAGASQFRALLCRIET
VQEGEALLLQAMERCQEATLRPEEAIKTTEEVDLLPEAGWA

Sequences:

>Translated_361_residues
MGLQLSPELQARLSQPLNIGGVKVLSRVFQAPLSGVTDQVFRGLVRRFAPHSLVYTEMVSATGLHYARQLPRIMEVGEGE
QPIGIQLFDCRPDFLAEAARMAVDEGATLVDINMGCPVNKITKNGGGSSLLRDPDTAARIVEAVANAVEVPVTVKTRLGW
SDQEINILDFAHRLQEAGAQLLTLHARTRAQGFNGKARWDWIRRVKQHLRIPVIANGDIFSTAAAVKCLEETGADGVMCS
RGTMGYPFLVGEIDHFLKTGQERPAPTPLERIQMALEHLRGLWEYKGERGIRQARKHMTWYIKDFAGASQFRALLCRIET
VQEGEALLLQAMERCQEATLRPEEAIKTTEEVDLLPEAGWA
>Mature_360_residues
GLQLSPELQARLSQPLNIGGVKVLSRVFQAPLSGVTDQVFRGLVRRFAPHSLVYTEMVSATGLHYARQLPRIMEVGEGEQ
PIGIQLFDCRPDFLAEAARMAVDEGATLVDINMGCPVNKITKNGGGSSLLRDPDTAARIVEAVANAVEVPVTVKTRLGWS
DQEINILDFAHRLQEAGAQLLTLHARTRAQGFNGKARWDWIRRVKQHLRIPVIANGDIFSTAAAVKCLEETGADGVMCSR
GTMGYPFLVGEIDHFLKTGQERPAPTPLERIQMALEHLRGLWEYKGERGIRQARKHMTWYIKDFAGASQFRALLCRIETV
QEGEALLLQAMERCQEATLRPEEAIKTTEEVDLLPEAGWA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI40807366, Length=340, Percent_Identity=30.8823529411765, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI239788483, Length=239, Percent_Identity=30.1255230125523, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI239788462, Length=246, Percent_Identity=29.6747967479675, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI31742496, Length=272, Percent_Identity=30.1470588235294, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI8923374, Length=155, Percent_Identity=31.6129032258064, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1789660, Length=310, Percent_Identity=40.3225806451613, Blast_Score=221, Evalue=8e-59,
Organism=Escherichia coli, GI1788462, Length=235, Percent_Identity=36.1702127659575, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI145693211, Length=309, Percent_Identity=26.537216828479, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17543114, Length=256, Percent_Identity=25.78125, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17507177, Length=239, Percent_Identity=28.8702928870293, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI25144369, Length=222, Percent_Identity=31.5315315315315, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323560, Length=345, Percent_Identity=26.9565217391304, Blast_Score=102, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6323433, Length=328, Percent_Identity=25.3048780487805, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323437, Length=254, Percent_Identity=25.5905511811024, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24580595, Length=259, Percent_Identity=33.5907335907336, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI19920448, Length=259, Percent_Identity=33.5907335907336, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI19921524, Length=229, Percent_Identity=31.4410480349345, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24585320, Length=250, Percent_Identity=30, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI45549423, Length=136, Percent_Identity=37.5, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 40032; Mature: 39900

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLQLSPELQARLSQPLNIGGVKVLSRVFQAPLSGVTDQVFRGLVRRFAPHSLVYTEMVS
CCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHH
ATGLHYARQLPRIMEVGEGEQPIGIQLFDCRPDFLAEAARMAVDEGATLVDINMGCPVNK
HHHHHHHHHCHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCEEEEEECCCCHHH
ITKNGGGSSLLRDPDTAARIVEAVANAVEVPVTVKTRLGWSDQEINILDFAHRLQEAGAQ
HHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHCCHH
LLTLHARTRAQGFNGKARWDWIRRVKQHLRIPVIANGDIFSTAAAVKCLEETGADGVMCS
HEEHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCCCCEEEC
RGTMGYPFLVGEIDHFLKTGQERPAPTPLERIQMALEHLRGLWEYKGERGIRQARKHMTW
CCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
YIKDFAGASQFRALLCRIETVQEGEALLLQAMERCQEATLRPEEAIKTTEEVDLLPEAGW
HHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCC
A
C
>Mature Secondary Structure 
GLQLSPELQARLSQPLNIGGVKVLSRVFQAPLSGVTDQVFRGLVRRFAPHSLVYTEMVS
CCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHH
ATGLHYARQLPRIMEVGEGEQPIGIQLFDCRPDFLAEAARMAVDEGATLVDINMGCPVNK
HHHHHHHHHCHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCEEEEEECCCCHHH
ITKNGGGSSLLRDPDTAARIVEAVANAVEVPVTVKTRLGWSDQEINILDFAHRLQEAGAQ
HHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHCCHH
LLTLHARTRAQGFNGKARWDWIRRVKQHLRIPVIANGDIFSTAAAVKCLEETGADGVMCS
HEEHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCCCCEEEC
RGTMGYPFLVGEIDHFLKTGQERPAPTPLERIQMALEHLRGLWEYKGERGIRQARKHMTW
CCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
YIKDFAGASQFRALLCRIETVQEGEALLLQAMERCQEATLRPEEAIKTTEEVDLLPEAGW
HHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]