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The map label for this gene is miaE [H]

Identifier: 86608878

GI number: 86608878

Start: 1477421

End: 1478002

Strand: Direct

Name: miaE [H]

Synonym: CYB_1409

Alternate gene names: 86608878

Gene position: 1477421-1478002 (Clockwise)

Preceding gene: 86608877

Following gene: 86608879

Centisome position: 48.49

GC content: 60.65

Gene sequence:

>582_bases
GTGCTTGCTCTAGAACTTGCTTCTCGTACCTCTGAGGACTGGATCGAACAGGCTACCCGGCACATCGATCTGGTCTTGCT
AGACCACGCCAATTGTGAGAAAAAAGCAGCGGGCAACGCCCTCAGCTTGCTGTTTCGCTACCCGGCTAATGGGCGTTTGG
TGGAAACCCTCTCGCCGCTGGCGCGGGAGGAGCTGCAGCATTTTGAGCGGGTGCATCGCCACCTGCAACGGCTGGGGATC
CCCATCAAGCCCTTGTCGGCTGCGCCCTATGCGGGTCGCCTAAACCAACACCTGCGCCGCTCTGAGCCAGAGCATCTGCT
CGACACCTTGCTGGTGGCAGCCATTATCGAGGCCCGCAGCCACGAACGCTTGGGCCTGTTGGGGATCCACGCGCCCGACC
CAGAGCTGCGGCAGTTTTATCTCTGGCTGGCGGCTGCGGAGGAGCGCCATTGGCAGCTTTACTTGGATTTGGCGTTGGAG
CAGTTTCCCGCCGATCAGGTGCAGGATCGGCTCGATACTCTACTCCAGGTGGAGGCCGAGATCTTGTCTTCCCTTCACCC
CGAACCCCGTGTTCACAGCTAG

Upstream 100 bases:

>100_bases
ATTGACCAAGTGTCATCCCCCGATCCCGGAGCTGACGGGGCGGGTTACTGTGGAGATTGTTCGTCCCTAGAACTTCTCTT
AACCCTTTCCTACCGACCCT

Downstream 100 bases:

>100_bases
CCCCACTCCGTTCTCCCCCCGCGGTGCAGCTCCCATGTTCCAAGCGACGCGACAGTGGTTGGCCTTCTGGTATACAACGG
TTACGGCAGTGTTGCTGCTG

Product: tRNA-(MS[2]IO[6]A)-hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MLALELASRTSEDWIEQATRHIDLVLLDHANCEKKAAGNALSLLFRYPANGRLVETLSPLAREELQHFERVHRHLQRLGI
PIKPLSAAPYAGRLNQHLRRSEPEHLLDTLLVAAIIEARSHERLGLLGIHAPDPELRQFYLWLAAAEERHWQLYLDLALE
QFPADQVQDRLDTLLQVEAEILSSLHPEPRVHS

Sequences:

>Translated_193_residues
MLALELASRTSEDWIEQATRHIDLVLLDHANCEKKAAGNALSLLFRYPANGRLVETLSPLAREELQHFERVHRHLQRLGI
PIKPLSAAPYAGRLNQHLRRSEPEHLLDTLLVAAIIEARSHERLGLLGIHAPDPELRQFYLWLAAAEERHWQLYLDLALE
QFPADQVQDRLDTLLQVEAEILSSLHPEPRVHS
>Mature_193_residues
MLALELASRTSEDWIEQATRHIDLVLLDHANCEKKAAGNALSLLFRYPANGRLVETLSPLAREELQHFERVHRHLQRLGI
PIKPLSAAPYAGRLNQHLRRSEPEHLLDTLLVAAIIEARSHERLGLLGIHAPDPELRQFYLWLAAAEERHWQLYLDLALE
QFPADQVQDRLDTLLQVEAEILSSLHPEPRVHS

Specific function: Production of the modified nucleoside 2-methylthio-cis- ribozeatin (MS[2]IO[6]A) found in some tRNAs. Catalyzes the oxygen-dependent transformation of MS[2]I[6]A into MS[2]IO[6]A [H]

COG id: COG4445

COG function: function code FJ; Hydroxylase for synthesis of 2-methylthio-cis-ribozeatin in tRNA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012347
- InterPro:   IPR010386 [H]

Pfam domain/function: PF06175 MiaE [H]

EC number: NA

Molecular weight: Translated: 22102; Mature: 22102

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLALELASRTSEDWIEQATRHIDLVLLDHANCEKKAAGNALSLLFRYPANGRLVETLSPL
CCCHHHHHCCCHHHHHHHHHHEEEEEEECCCCCHHHCCCHHHHHEECCCCCCHHHHHHHH
AREELQHFERVHRHLQRLGIPIKPLSAAPYAGRLNQHLRRSEPEHLLDTLLVAAIIEARS
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
HERLGLLGIHAPDPELRQFYLWLAAAEERHWQLYLDLALEQFPADQVQDRLDTLLQVEAE
CCCCCEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
ILSSLHPEPRVHS
HHHCCCCCCCCCC
>Mature Secondary Structure
MLALELASRTSEDWIEQATRHIDLVLLDHANCEKKAAGNALSLLFRYPANGRLVETLSPL
CCCHHHHHCCCHHHHHHHHHHEEEEEEECCCCCHHHCCCHHHHHEECCCCCCHHHHHHHH
AREELQHFERVHRHLQRLGIPIKPLSAAPYAGRLNQHLRRSEPEHLLDTLLVAAIIEARS
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
HERLGLLGIHAPDPELRQFYLWLAAAEERHWQLYLDLALEQFPADQVQDRLDTLLQVEAE
CCCCCEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
ILSSLHPEPRVHS
HHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253666; 11677609 [H]