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The map label for this gene is mraW

Identifier: 86608801

GI number: 86608801

Start: 1388992

End: 1389951

Strand: Direct

Name: mraW

Synonym: CYB_1327

Alternate gene names: 86608801

Gene position: 1388992-1389951 (Clockwise)

Preceding gene: 86608798

Following gene: 86608802

Centisome position: 45.59

GC content: 61.35

Gene sequence:

>960_bases
TTGCAGATACTGAACTTTGTAGCAGCAGAAATAGAACCCCCGCAGACCGTGCTTGGCAAGGTGTATCAACACGATTCCGT
TTTGACCGAGGAGGTGATGGCCTACCTGCAACCCCAGGGGGGCGGTATTTTTTTGGATGTGACGTTGGGAGGGGGTGGGC
ACAGCTTGGCGCTGCTGCGGGGGGGAGCTGCCCGAGTGGTGGGGTTGGATCGGGATCCGGCGGCTCTACAGGCGGCGCAA
GCGCGTTTTGCAGCAGAAGGGATCCCCGGCAGCCGCATCCAACTGTGGCACCTCAATTTTGCCGATTTCGATCTGCAGCA
GCACGGCTTTCGGGATGAGCAGGGGCAGGGGATCCCCTTCGATGGCATCGTGGCCGACTTGGGGGTGAGTTCGCCGCAAC
TGGACTGCCCTGAGCGGGGATTTAGCTTTCGCGGAGAGGGGCCGCTGGATATGCGCATGGATCCCAGTGCCGCTCAGGAA
ACCGCTGCCGATTGGGTTAATCATCGCCCGGTCGAAGAGTTGATCGAGATTTTCACCCGTTACGGCGAAGAGCGATTTGC
CCGCCGCATTGCCCATCACATCGAGCAGGCTCGTCCCCTCGCCACAACTACCCAGTTGGCCCAGGTGGTTTGGCAGGCGG
TACCTCCGGCAGCTCGGCGAGGCCGCATCCACCCGGCCACTCGGGTCTTTCAGGCGTTGCGCATTGCCGTGAACCGAGAA
CTGGAGGCTTTAGAAACCTTGCTGGCCCAAGCCCCCACTTGGTTGAAACCCGGCGGCAGGTTGGCCGTGATCAGCTTCCA
TAGCCTTGAAGATCGCTTGGTGAAATGGGCCTTCCGCAGGGATCCCCGTTGGCAGGTGCTCACCCCCAAGCCCTTGTCCC
CTTCCGAACTGGAACGGCAACGCAATCCACGGGCCCGCTCTGCCAAACTGCGAGTGGCTGCCCGCAGCAGTCAGATGTAG

Upstream 100 bases:

>100_bases
CCCAAGACAGCCTGTCTTTTGACGGCCACCCCTCTTTTCTCAGAGGATCACCTTAACAACATGGATTCCTCGGTCGGGGC
CTGGCCTCGGCCTTTTTTTT

Downstream 100 bases:

>100_bases
GTTTAGGTTGGTTCTCCCGCTCTCAAGAGATCGGCTACTGTGAAGCGGACAGCTGTCCTGCTGAGGCCGGGAATCGGGAG
AAGGGGGTTGCTGTTGGGGG

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MQILNFVAAEIEPPQTVLGKVYQHDSVLTEEVMAYLQPQGGGIFLDVTLGGGGHSLALLRGGAARVVGLDRDPAALQAAQ
ARFAAEGIPGSRIQLWHLNFADFDLQQHGFRDEQGQGIPFDGIVADLGVSSPQLDCPERGFSFRGEGPLDMRMDPSAAQE
TAADWVNHRPVEELIEIFTRYGEERFARRIAHHIEQARPLATTTQLAQVVWQAVPPAARRGRIHPATRVFQALRIAVNRE
LEALETLLAQAPTWLKPGGRLAVISFHSLEDRLVKWAFRRDPRWQVLTPKPLSPSELERQRNPRARSAKLRVAARSSQM

Sequences:

>Translated_319_residues
MQILNFVAAEIEPPQTVLGKVYQHDSVLTEEVMAYLQPQGGGIFLDVTLGGGGHSLALLRGGAARVVGLDRDPAALQAAQ
ARFAAEGIPGSRIQLWHLNFADFDLQQHGFRDEQGQGIPFDGIVADLGVSSPQLDCPERGFSFRGEGPLDMRMDPSAAQE
TAADWVNHRPVEELIEIFTRYGEERFARRIAHHIEQARPLATTTQLAQVVWQAVPPAARRGRIHPATRVFQALRIAVNRE
LEALETLLAQAPTWLKPGGRLAVISFHSLEDRLVKWAFRRDPRWQVLTPKPLSPSELERQRNPRARSAKLRVAARSSQM
>Mature_319_residues
MQILNFVAAEIEPPQTVLGKVYQHDSVLTEEVMAYLQPQGGGIFLDVTLGGGGHSLALLRGGAARVVGLDRDPAALQAAQ
ARFAAEGIPGSRIQLWHLNFADFDLQQHGFRDEQGQGIPFDGIVADLGVSSPQLDCPERGFSFRGEGPLDMRMDPSAAQE
TAADWVNHRPVEELIEIFTRYGEERFARRIAHHIEQARPLATTTQLAQVVWQAVPPAARRGRIHPATRVFQALRIAVNRE
LEALETLLAQAPTWLKPGGRLAVISFHSLEDRLVKWAFRRDPRWQVLTPKPLSPSELERQRNPRARSAKLRVAARSSQM

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=342, Percent_Identity=42.1052631578947, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI165377202, Length=194, Percent_Identity=44.8453608247423, Blast_Score=128, Evalue=7e-30,
Organism=Escherichia coli, GI1786270, Length=321, Percent_Identity=41.4330218068536, Blast_Score=203, Evalue=1e-53,
Organism=Drosophila melanogaster, GI62472493, Length=336, Percent_Identity=35.1190476190476, Blast_Score=164, Evalue=8e-41,
Organism=Drosophila melanogaster, GI28571637, Length=336, Percent_Identity=35.1190476190476, Blast_Score=164, Evalue=8e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_SYNJB (Q2JLV1)

Other databases:

- EMBL:   CP000240
- RefSeq:   YP_477563.1
- ProteinModelPortal:   Q2JLV1
- STRING:   Q2JLV1
- GeneID:   3901157
- GenomeReviews:   CP000240_GR
- KEGG:   cyb:CYB_1327
- TIGR:   CYB_1327
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- BioCyc:   SSP321332:CYB_1327-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 35441; Mature: 35441

Theoretical pI: Translated: 8.53; Mature: 8.53

Prosite motif: NA

Important sites: BINDING 70-70 BINDING 100-100 BINDING 126-126 BINDING 133-133

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQILNFVAAEIEPPQTVLGKVYQHDSVLTEEVMAYLQPQGGGIFLDVTLGGGGHSLALLR
CCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEEEE
GGAARVVGLDRDPAALQAAQARFAAEGIPGSRIQLWHLNFADFDLQQHGFRDEQGQGIPF
CCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHCCCCCCCCCCCCC
DGIVADLGVSSPQLDCPERGFSFRGEGPLDMRMDPSAAQETAADWVNHRPVEELIEIFTR
CCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
YGEERFARRIAHHIEQARPLATTTQLAQVVWQAVPPAARRGRIHPATRVFQALRIAVNRE
HCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHCCH
LEALETLLAQAPTWLKPGGRLAVISFHSLEDRLVKWAFRRDPRWQVLTPKPLSPSELERQ
HHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHH
RNPRARSAKLRVAARSSQM
CCCCCHHHEEEEECCCCCC
>Mature Secondary Structure
MQILNFVAAEIEPPQTVLGKVYQHDSVLTEEVMAYLQPQGGGIFLDVTLGGGGHSLALLR
CCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEEEE
GGAARVVGLDRDPAALQAAQARFAAEGIPGSRIQLWHLNFADFDLQQHGFRDEQGQGIPF
CCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHCCCCCCCCCCCCC
DGIVADLGVSSPQLDCPERGFSFRGEGPLDMRMDPSAAQETAADWVNHRPVEELIEIFTR
CCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
YGEERFARRIAHHIEQARPLATTTQLAQVVWQAVPPAARRGRIHPATRVFQALRIAVNRE
HCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHCCH
LEALETLLAQAPTWLKPGGRLAVISFHSLEDRLVKWAFRRDPRWQVLTPKPLSPSELERQ
HHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHH
RNPRARSAKLRVAARSSQM
CCCCCHHHEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA