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The map label for this gene is lipA-1 [H]

Identifier: 86608595

GI number: 86608595

Start: 1155328

End: 1156215

Strand: Reverse

Name: lipA-1 [H]

Synonym: CYB_1117

Alternate gene names: 86608595

Gene position: 1156215-1155328 (Counterclockwise)

Preceding gene: 86608598

Following gene: 86608591

Centisome position: 37.95

GC content: 62.05

Gene sequence:

>888_bases
TTGACCGTTGTTTCTTCTGGTAGCAAACCCTCCTGGCTGCGGGCCAAGGCCCCTCAACGGGTGGGATCCGTCAAACAACT
GCTGCGGGACTTGGGCCTGAACACGGTTTGCGAAGAGGCTTCCTGTCCCAACCTGGGGGAGTGTTTTGCCAACGGCACCG
CCACCTTTTTGATCATGGGCCCGGCCTGTACCCGCGCCTGCCCCTACTGCGATATCGACTTCACCAAACGCCCTCTGCCC
CTGGATGCCACTGAACCCCGGCGGGTGGCAGAAGCAGTGGAACGCTTGGGATTGCGCCATGCGGTGATTACGTCGGTGAA
TCGGGACGACCTGCCCGATGGGGGAGCCAGCCAGTTTGTGGCCTGCATTGCGGAAATCCGCCGCCGCATGCCCCAGACCA
CCATCGAGGTCTTGATCCCAGACCTATGTGGTAACTGGCAGGCGCTGCAGCAGATCTTGGCCGCCCGACCGACGGTGCTC
AACCACAATACCGAAACGGTGCCCCGCCTCTATCGCCGTGTACGCCCGCAGGGGAATTACGAGCGCTCCCTCAAACTGTT
GGAGTTGGCACGGCAATGGGATCCTGGCCTGTACACCAAGTCGGGGGTGATGTTGGGCCTGGGAGAAACTGCCGAGGAGA
TCTTGCAGGTGATGCGGGATCTGCGCCGGGCAGGGTGTGACATCCTCACGCTCGGCCAATACCTGGCGCCCAGCCCCAAA
CACCTGCCGGTGGAGCGCTACGTTCCCCCCGAAGAGTTCGACCATTGGCGACGGGTGGGAGAGGAACTGGGCTTTTTGCA
GGTGGTGTCTTCGCCCCTAACCCGCAGCTCCTACCATGCCGAGGCTGTGCAAGAGTTGATGCAGCGCTTTCCCAGGAGGA
GCTCCTGA

Upstream 100 bases:

>100_bases
AATTGGGCCGGGAAATTGGCTTTGTCCAAATTCCAACGGGGCCAAATGGCAGGCTAAGCTGAGTGATACCGTCGCTGCTG
TTTTGTGTTGGAGCACTTCT

Downstream 100 bases:

>100_bases
GCGCTGGGGCCTCGCCGGCTGCCCGACCTTGTGGGTTGCGCGAGAGAAGAACCCTACTCTGAGATTGGCTCAAGGCCGGG
TTTCAGGGCGGCTACAACGT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn 1; Lipoate synthase 1; Lipoic acid synthase 1; Sulfur insertion protein lipA1 [H]

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MTVVSSGSKPSWLRAKAPQRVGSVKQLLRDLGLNTVCEEASCPNLGECFANGTATFLIMGPACTRACPYCDIDFTKRPLP
LDATEPRRVAEAVERLGLRHAVITSVNRDDLPDGGASQFVACIAEIRRRMPQTTIEVLIPDLCGNWQALQQILAARPTVL
NHNTETVPRLYRRVRPQGNYERSLKLLELARQWDPGLYTKSGVMLGLGETAEEILQVMRDLRRAGCDILTLGQYLAPSPK
HLPVERYVPPEEFDHWRRVGEELGFLQVVSSPLTRSSYHAEAVQELMQRFPRRSS

Sequences:

>Translated_295_residues
MTVVSSGSKPSWLRAKAPQRVGSVKQLLRDLGLNTVCEEASCPNLGECFANGTATFLIMGPACTRACPYCDIDFTKRPLP
LDATEPRRVAEAVERLGLRHAVITSVNRDDLPDGGASQFVACIAEIRRRMPQTTIEVLIPDLCGNWQALQQILAARPTVL
NHNTETVPRLYRRVRPQGNYERSLKLLELARQWDPGLYTKSGVMLGLGETAEEILQVMRDLRRAGCDILTLGQYLAPSPK
HLPVERYVPPEEFDHWRRVGEELGFLQVVSSPLTRSSYHAEAVQELMQRFPRRSS
>Mature_294_residues
TVVSSGSKPSWLRAKAPQRVGSVKQLLRDLGLNTVCEEASCPNLGECFANGTATFLIMGPACTRACPYCDIDFTKRPLPL
DATEPRRVAEAVERLGLRHAVITSVNRDDLPDGGASQFVACIAEIRRRMPQTTIEVLIPDLCGNWQALQQILAARPTVLN
HNTETVPRLYRRVRPQGNYERSLKLLELARQWDPGLYTKSGVMLGLGETAEEILQVMRDLRRAGCDILTLGQYLAPSPKH
LPVERYVPPEEFDHWRRVGEELGFLQVVSSPLTRSSYHAEAVQELMQRFPRRSS

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=279, Percent_Identity=44.0860215053763, Blast_Score=263, Evalue=1e-70,
Organism=Homo sapiens, GI37577164, Length=244, Percent_Identity=42.2131147540984, Blast_Score=217, Evalue=8e-57,
Organism=Escherichia coli, GI1786846, Length=277, Percent_Identity=53.0685920577617, Blast_Score=301, Evalue=3e-83,
Organism=Caenorhabditis elegans, GI32564533, Length=252, Percent_Identity=44.047619047619, Blast_Score=228, Evalue=4e-60,
Organism=Saccharomyces cerevisiae, GI6324770, Length=279, Percent_Identity=41.9354838709677, Blast_Score=237, Evalue=2e-63,
Organism=Drosophila melanogaster, GI221513272, Length=279, Percent_Identity=45.8781362007168, Blast_Score=259, Evalue=2e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 33046; Mature: 32915

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVVSSGSKPSWLRAKAPQRVGSVKQLLRDLGLNTVCEEASCPNLGECFANGTATFLIMG
CCCCCCCCCCCHHHHHCHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHCCCCEEEEEEC
PACTRACPYCDIDFTKRPLPLDATEPRRVAEAVERLGLRHAVITSVNRDDLPDGGASQFV
CHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
ACIAEIRRRMPQTTIEVLIPDLCGNWQALQQILAARPTVLNHNTETVPRLYRRVRPQGNY
HHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCH
ERSLKLLELARQWDPGLYTKSGVMLGLGETAEEILQVMRDLRRAGCDILTLGQYLAPSPK
HHHHHHHHHHHHCCCCCEECCCEEEECCHHHHHHHHHHHHHHHCCCCEEEHHHHHCCCCC
HLPVERYVPPEEFDHWRRVGEELGFLQVVSSPLTRSSYHAEAVQELMQRFPRRSS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TVVSSGSKPSWLRAKAPQRVGSVKQLLRDLGLNTVCEEASCPNLGECFANGTATFLIMG
CCCCCCCCCCHHHHHCHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHCCCCEEEEEEC
PACTRACPYCDIDFTKRPLPLDATEPRRVAEAVERLGLRHAVITSVNRDDLPDGGASQFV
CHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
ACIAEIRRRMPQTTIEVLIPDLCGNWQALQQILAARPTVLNHNTETVPRLYRRVRPQGNY
HHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCH
ERSLKLLELARQWDPGLYTKSGVMLGLGETAEEILQVMRDLRRAGCDILTLGQYLAPSPK
HHHHHHHHHHHHCCCCCEECCCEEEECCHHHHHHHHHHHHHHHCCCCEEEHHHHHCCCCC
HLPVERYVPPEEFDHWRRVGEELGFLQVVSSPLTRSSYHAEAVQELMQRFPRRSS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12240834 [H]