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The map label for this gene is gshB [H]

Identifier: 86608528

GI number: 86608528

Start: 1088232

End: 1089179

Strand: Reverse

Name: gshB [H]

Synonym: CYB_1049

Alternate gene names: 86608528

Gene position: 1089179-1088232 (Counterclockwise)

Preceding gene: 86608530

Following gene: 86608525

Centisome position: 35.75

GC content: 56.12

Gene sequence:

>948_bases
ATGCAGGTAGCTTTTATTGTCGATCCATTGCCTCAGTTGCAGGTGGGCCATGACACCAGCATTGCCCTGATGGAAGCTCT
GCAGAAACGGGGACACCGCATTTTCTGCCTGGGCCTGGCGGATCTCTACTGGGATCAAGGGGAAACTTGGGGATCCCTTG
AGGAAATCCGGTTGGATCTGCAAGCGGATCCCTGGTATGAGGTCAAAGACCGGCAACTGCTGCCCCTGAGCCAAATGCAG
GCGGTGTGGATGCGCAAGGATCCGCCGGTAGATGCGGCCTATCTCTATGCCACTTACCTTTTGGATCGTCTCTCTCCCAG
CCGCACACTGGTGCTCAATCATCCTGCCGGGATCCGCTCGGCCAACGAAAAGCTCTATGCCCTGCAATTTCAAGAGTGGA
TCCCGCGCACCCGCGTCAGCAGCAACAAGCGTCTGCTGCGTCAGTTCGTCGAACAGGAAGGACAGGCGGTGCTCAAGCCC
TTGGGTGGCAAAGGTGGGGAAGGGATCCTGAGGGTCAATGCCGGGGATCCAAACCTCAACTCGTTGATTGAAATGAGCAC
CCAATTTGGTCGGCTGCCTGTGATGGTGCAGGAGTATTTGCCACAGGCGGCCGCAGGAGATAAACGCATTTTGTTGCTGG
AAGGAGAACCTTTGGGCGCCGTCAATCGGATCCCTGGAGAGGGCGATTTTCGGGGCAATGTTGCTGCCGGCGGTCGAGTG
GAAAAAACCAAGATTACCGAAAAAGAACGGGCTCTCTGCGGGGCTTTGGCACCGGTGCTCAGGCAGGAGAAGCTGTATTT
TGTCGGGATTGATGTTATCGGGGAACGACTAACCGAAGTGAACGTGACCAGCCCAACAATGCTGCGAGAACTCTGTCAGT
TGGAAGGAGTTGACCTGGCAGATCAAGTGGCTCAGTGGCTGGAAAAGAAGGTGCCCGTCAAGGGCTGA

Upstream 100 bases:

>100_bases
AGGGGGCCTTCTCTTGTGGAAACAGCCTACCGGGGGAGAGCCATCGGCTCCGATTTGTTGCATACTCGGGGCAGCCTTTC
GTCAGTGAACCACACCTCCC

Downstream 100 bases:

>100_bases
ATGACTACCCACCAGGATGTTTCTGGGATCCGGCGCGTCTGTTGCCTGACGGGGTTCCCGTCTATTTAGGGAGTCTATAG
AGATCAAATTATAGCTTGGA

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MQVAFIVDPLPQLQVGHDTSIALMEALQKRGHRIFCLGLADLYWDQGETWGSLEEIRLDLQADPWYEVKDRQLLPLSQMQ
AVWMRKDPPVDAAYLYATYLLDRLSPSRTLVLNHPAGIRSANEKLYALQFQEWIPRTRVSSNKRLLRQFVEQEGQAVLKP
LGGKGGEGILRVNAGDPNLNSLIEMSTQFGRLPVMVQEYLPQAAAGDKRILLLEGEPLGAVNRIPGEGDFRGNVAAGGRV
EKTKITEKERALCGALAPVLRQEKLYFVGIDVIGERLTEVNVTSPTMLRELCQLEGVDLADQVAQWLEKKVPVKG

Sequences:

>Translated_315_residues
MQVAFIVDPLPQLQVGHDTSIALMEALQKRGHRIFCLGLADLYWDQGETWGSLEEIRLDLQADPWYEVKDRQLLPLSQMQ
AVWMRKDPPVDAAYLYATYLLDRLSPSRTLVLNHPAGIRSANEKLYALQFQEWIPRTRVSSNKRLLRQFVEQEGQAVLKP
LGGKGGEGILRVNAGDPNLNSLIEMSTQFGRLPVMVQEYLPQAAAGDKRILLLEGEPLGAVNRIPGEGDFRGNVAAGGRV
EKTKITEKERALCGALAPVLRQEKLYFVGIDVIGERLTEVNVTSPTMLRELCQLEGVDLADQVAQWLEKKVPVKG
>Mature_315_residues
MQVAFIVDPLPQLQVGHDTSIALMEALQKRGHRIFCLGLADLYWDQGETWGSLEEIRLDLQADPWYEVKDRQLLPLSQMQ
AVWMRKDPPVDAAYLYATYLLDRLSPSRTLVLNHPAGIRSANEKLYALQFQEWIPRTRVSSNKRLLRQFVEQEGQAVLKP
LGGKGGEGILRVNAGDPNLNSLIEMSTQFGRLPVMVQEYLPQAAAGDKRILLLEGEPLGAVNRIPGEGDFRGNVAAGGRV
EKTKITEKERALCGALAPVLRQEKLYFVGIDVIGERLTEVNVTSPTMLRELCQLEGVDLADQVAQWLEKKVPVKG

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=292, Percent_Identity=39.7260273972603, Blast_Score=240, Evalue=8e-65,
Organism=Escherichia coli, GI1787076, Length=246, Percent_Identity=26.4227642276423, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 35191; Mature: 35191

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVAFIVDPLPQLQVGHDTSIALMEALQKRGHRIFCLGLADLYWDQGETWGSLEEIRLDL
CEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEHHHHHCCCCCCCCHHHEEEEC
QADPWYEVKDRQLLPLSQMQAVWMRKDPPVDAAYLYATYLLDRLSPSRTLVLNHPAGIRS
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCC
ANEKLYALQFQEWIPRTRVSSNKRLLRQFVEQEGQAVLKPLGGKGGEGILRVNAGDPNLN
CCCCEEEEEHHHHCCCHHCCCCHHHHHHHHHHCCCEEHHCCCCCCCCCEEEEECCCCCHH
SLIEMSTQFGRLPVMVQEYLPQAAAGDKRILLLEGEPLGAVNRIPGEGDFRGNVAAGGRV
HHHHHHHHHCCCCHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEE
EKTKITEKERALCGALAPVLRQEKLYFVGIDVIGERLTEVNVTSPTMLRELCQLEGVDLA
EHHHCCHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHH
DQVAQWLEKKVPVKG
HHHHHHHHHCCCCCC
>Mature Secondary Structure
MQVAFIVDPLPQLQVGHDTSIALMEALQKRGHRIFCLGLADLYWDQGETWGSLEEIRLDL
CEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEHHHHHCCCCCCCCHHHEEEEC
QADPWYEVKDRQLLPLSQMQAVWMRKDPPVDAAYLYATYLLDRLSPSRTLVLNHPAGIRS
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCC
ANEKLYALQFQEWIPRTRVSSNKRLLRQFVEQEGQAVLKPLGGKGGEGILRVNAGDPNLN
CCCCEEEEEHHHHCCCHHCCCCHHHHHHHHHHCCCEEHHCCCCCCCCCEEEEECCCCCHH
SLIEMSTQFGRLPVMVQEYLPQAAAGDKRILLLEGEPLGAVNRIPGEGDFRGNVAAGGRV
HHHHHHHHHCCCCHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEE
EKTKITEKERALCGALAPVLRQEKLYFVGIDVIGERLTEVNVTSPTMLRELCQLEGVDLA
EHHHCCHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHH
DQVAQWLEKKVPVKG
HHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12240834 [H]