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The map label for this gene is cobA [H]

Identifier: 86608519

GI number: 86608519

Start: 1081523

End: 1082305

Strand: Direct

Name: cobA [H]

Synonym: CYB_1040

Alternate gene names: 86608519

Gene position: 1081523-1082305 (Clockwise)

Preceding gene: 86608518

Following gene: 86608520

Centisome position: 35.5

GC content: 62.58

Gene sequence:

>783_bases
ATGGGCAAAGTGTATTTGGTGGGGGCAGGGCCTGGGGATCCAGGGCTGCTGACGGTGAAGGGAAAGGCGCTACTCGCCCA
CGCGGATGTGGTCATCTACGATGCTCTGGTGGGATCCTCCATCCTGGAGCTGATCCCGCCGACGGCAGAACGGATCTATG
CTGGCAAACACTGCGGCGCTCACACCTTGCCCCAGGAGCAGATCACAGCCTTGCTGATGGAGAAAGCTCGGACGGCAGCC
GTGGTGGTGCGGCTCAAAGGGGGGGATCCCTTCATCTTTGGGCGAGGCGGCGAGGAGATGTTGGCTCTGGTGCAGGCCGG
GATCCCAGTGGAGGTGGTGCCGGGAATTACGGCGGGCATTGCCGCCAGCGCCTATGCCGGGATCCCTCTCACCCATCGCG
ACCTCAGCTCCTCAGTTGCTTTTGTTACCGGACATGAGGCGGCAGGGAAATACCGCCCTCAGGTCAATTGGCGGGCTTTG
GCCGGGGCGGTGGAAACCCTGGTGATCTACATGGGCATGCACAACCTCAGCCACATTGCCGCTGAATTGTTGGCCGGAGG
GAAAGCTCCCGACACCCCGGTTGCCCTGATCCGCTGGGGCACCACCCCTGAGCAAGAGATTCATCTGCTCCGCCTGCAGA
CTCTGGCTGGGCAGCCCTCGCCCGTAGCACCGCCTTCAATTGTTGTGGTGGGAGCGGTGGTCAGCCTGGCCAGCTTGTTG
CCCACTTTGCGTCCGCCAGTCTCGTATTGCTCCGCAGCGCTTGCGCAACCGGAATCTCCATGA

Upstream 100 bases:

>100_bases
GAGACACTCCAGGCTTTTAGCCCGGCGCTGGAATAGCCGAATCTTTCCCGTTCCCTCAAAATGGATATTTCCAACAGCAA
TGACGCGACCAGGACTTGAG

Downstream 100 bases:

>100_bases
ACGCCTTCTTTCGCAAGTTGGGTGCCCTCTTCTCCGCCTACTACGCCTACATGCTGGAGTACCGGGCCGAGCTGATCCTG
TGGGTGTTGGCCGGATCCCT

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MGKVYLVGAGPGDPGLLTVKGKALLAHADVVIYDALVGSSILELIPPTAERIYAGKHCGAHTLPQEQITALLMEKARTAA
VVVRLKGGDPFIFGRGGEEMLALVQAGIPVEVVPGITAGIAASAYAGIPLTHRDLSSSVAFVTGHEAAGKYRPQVNWRAL
AGAVETLVIYMGMHNLSHIAAELLAGGKAPDTPVALIRWGTTPEQEIHLLRLQTLAGQPSPVAPPSIVVVGAVVSLASLL
PTLRPPVSYCSAALAQPESP

Sequences:

>Translated_260_residues
MGKVYLVGAGPGDPGLLTVKGKALLAHADVVIYDALVGSSILELIPPTAERIYAGKHCGAHTLPQEQITALLMEKARTAA
VVVRLKGGDPFIFGRGGEEMLALVQAGIPVEVVPGITAGIAASAYAGIPLTHRDLSSSVAFVTGHEAAGKYRPQVNWRAL
AGAVETLVIYMGMHNLSHIAAELLAGGKAPDTPVALIRWGTTPEQEIHLLRLQTLAGQPSPVAPPSIVVVGAVVSLASLL
PTLRPPVSYCSAALAQPESP
>Mature_259_residues
GKVYLVGAGPGDPGLLTVKGKALLAHADVVIYDALVGSSILELIPPTAERIYAGKHCGAHTLPQEQITALLMEKARTAAV
VVRLKGGDPFIFGRGGEEMLALVQAGIPVEVVPGITAGIAASAYAGIPLTHRDLSSSVAFVTGHEAAGKYRPQVNWRALA
GAVETLVIYMGMHNLSHIAAELLAGGKAPDTPVALIRWGTTPEQEIHLLRLQTLAGQPSPVAPPSIVVVGAVVSLASLLP
TLRPPVSYCSAALAQPESP

Specific function: Catalyzes both methylations at C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=239, Percent_Identity=46.8619246861925, Blast_Score=218, Evalue=3e-58,
Organism=Saccharomyces cerevisiae, GI6322922, Length=240, Percent_Identity=35.8333333333333, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 26868; Mature: 26736

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKVYLVGAGPGDPGLLTVKGKALLAHADVVIYDALVGSSILELIPPTAERIYAGKHCGA
CCEEEEEECCCCCCCEEEEECCEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCC
HTLPQEQITALLMEKARTAAVVVRLKGGDPFIFGRGGEEMLALVQAGIPVEVVPGITAGI
CCCCHHHHHHHHHHHCCEEEEEEEECCCCEEEEECCHHHHHHHHHCCCCEEECCCCHHHH
AASAYAGIPLTHRDLSSSVAFVTGHEAAGKYRPQVNWRALAGAVETLVIYMGMHNLSHIA
HHHHHCCCCEECCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AELLAGGKAPDTPVALIRWGTTPEQEIHLLRLQTLAGQPSPVAPPSIVVVGAVVSLASLL
HHHHHCCCCCCCCEEEEEECCCCHHHHHHHEEHHHCCCCCCCCCCHHHHHHHHHHHHHHH
PTLRPPVSYCSAALAQPESP
HHCCCCHHHHHHHHCCCCCC
>Mature Secondary Structure 
GKVYLVGAGPGDPGLLTVKGKALLAHADVVIYDALVGSSILELIPPTAERIYAGKHCGA
CEEEEEECCCCCCCEEEEECCEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCC
HTLPQEQITALLMEKARTAAVVVRLKGGDPFIFGRGGEEMLALVQAGIPVEVVPGITAGI
CCCCHHHHHHHHHHHCCEEEEEEEECCCCEEEEECCHHHHHHHHHCCCCEEECCCCHHHH
AASAYAGIPLTHRDLSSSVAFVTGHEAAGKYRPQVNWRALAGAVETLVIYMGMHNLSHIA
HHHHHCCCCEECCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AELLAGGKAPDTPVALIRWGTTPEQEIHLLRLQTLAGQPSPVAPPSIVVVGAVVSLASLL
HHHHHCCCCCCCCEEEEEECCCCHHHHHHHEEHHHCCCCCCCCCCHHHHHHHHHHHHHHH
PTLRPPVSYCSAALAQPESP
HHCCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]