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The map label for this gene is nth [H]

Identifier: 86608470

GI number: 86608470

Start: 1026867

End: 1027571

Strand: Direct

Name: nth [H]

Synonym: CYB_0991

Alternate gene names: 86608470

Gene position: 1026867-1027571 (Clockwise)

Preceding gene: 86608469

Following gene: 86608471

Centisome position: 33.7

GC content: 60.71

Gene sequence:

>705_bases
ATGGCCAGCAAACGGGCCTTGCGCCAGCGAGCTTTGGAGATCCTGCTCCGGCTGAAGCGCCACTACCCCAACTCCACCTG
TGCCCTCCACTACCGGACGCCGCTGCAACTTTTGGTGGCAACCATCCTCTCGGCTCAGTGTACGGATGAGCGGGTGAATC
AGGTAACGCCAGAGCTGTTTCGTCGCTTCCCGGATGCCCAGGCCCTGGCGACAGCGCCGCGAGAGGAGATCGAGGCTCTC
ATCCATTCCACCGGCTTCTATCGCAACAAGGCCAAGCACATTCAGGAAGCCTGCCGCAGGATCTTAAGCCATTTCGGTGG
CCAAGTGCCCCGCACCATGCCGGAGCTGCTCACCCTACCCGGCGTAGCTCGCAAGACGGCCAATGTGGTGTTGGCTCATG
CTTTTGGCATCAATGCCGGGGTTACCGTCGATACCCATGTTAAGCGCCTCAGCCGCCGCCTCGGCCTCACTGAGCACGAG
GATCCCGTGCGCATCGAAAAGGATCTGATGCAGTTGTTGCCGCAAGCGGACTGGGAGAACTGGTCTATCCGTCTGATCGA
CCACGGGCGGGCCATCTGCACTGCCCGCAGGCCTCTGTGCCAGCAATGTTTTTTGGCGGATCTCTGCCCCAGCGCTCCCC
TCCTGAGACCGGCGGCACCGGATGAGAAAATGGATAAAAAAGCTACCAAAGCCTCGCCAACCTGA

Upstream 100 bases:

>100_bases
GCGGGTGATGCAGACGGGCTTGGTGTTGTTGCTGGGATTGGGGGTGGTGCTGATCTTCAAAGACACCCTCGCCATTGTCA
CCGGGTCGGGCTAGGGGAAG

Downstream 100 bases:

>100_bases
GAGGATGGTTTCTGGGCGCCCTGTATGGCTGAGCCGGCAGGGATCCCTCCCTTGCAGCACTTGTTTTGGCCGCCTCTCGC
CCTAGAATAAGCTCCATGCA

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MASKRALRQRALEILLRLKRHYPNSTCALHYRTPLQLLVATILSAQCTDERVNQVTPELFRRFPDAQALATAPREEIEAL
IHSTGFYRNKAKHIQEACRRILSHFGGQVPRTMPELLTLPGVARKTANVVLAHAFGINAGVTVDTHVKRLSRRLGLTEHE
DPVRIEKDLMQLLPQADWENWSIRLIDHGRAICTARRPLCQQCFLADLCPSAPLLRPAAPDEKMDKKATKASPT

Sequences:

>Translated_234_residues
MASKRALRQRALEILLRLKRHYPNSTCALHYRTPLQLLVATILSAQCTDERVNQVTPELFRRFPDAQALATAPREEIEAL
IHSTGFYRNKAKHIQEACRRILSHFGGQVPRTMPELLTLPGVARKTANVVLAHAFGINAGVTVDTHVKRLSRRLGLTEHE
DPVRIEKDLMQLLPQADWENWSIRLIDHGRAICTARRPLCQQCFLADLCPSAPLLRPAAPDEKMDKKATKASPT
>Mature_233_residues
ASKRALRQRALEILLRLKRHYPNSTCALHYRTPLQLLVATILSAQCTDERVNQVTPELFRRFPDAQALATAPREEIEALI
HSTGFYRNKAKHIQEACRRILSHFGGQVPRTMPELLTLPGVARKTANVVLAHAFGINAGVTVDTHVKRLSRRLGLTEHED
PVRIEKDLMQLLPQADWENWSIRLIDHGRAICTARRPLCQQCFLADLCPSAPLLRPAAPDEKMDKKATKASPT

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=179, Percent_Identity=31.2849162011173, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI115298648, Length=179, Percent_Identity=30.7262569832402, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI6912520, Length=179, Percent_Identity=30.7262569832402, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI115298654, Length=179, Percent_Identity=30.7262569832402, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI115298652, Length=179, Percent_Identity=30.7262569832402, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI190358497, Length=179, Percent_Identity=30.7262569832402, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI115298650, Length=179, Percent_Identity=30.7262569832402, Blast_Score=80, Evalue=1e-15,
Organism=Escherichia coli, GI1787920, Length=205, Percent_Identity=38.0487804878049, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1789331, Length=186, Percent_Identity=28.494623655914, Blast_Score=80, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17554540, Length=183, Percent_Identity=34.4262295081967, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6324530, Length=190, Percent_Identity=30, Blast_Score=91, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6319304, Length=178, Percent_Identity=31.4606741573034, Blast_Score=86, Evalue=4e-18,
Organism=Drosophila melanogaster, GI45550361, Length=180, Percent_Identity=33.3333333333333, Blast_Score=112, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 26387; Mature: 26256

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASKRALRQRALEILLRLKRHYPNSTCALHYRTPLQLLVATILSAQCTDERVNQVTPELF
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHH
RRFPDAQALATAPREEIEALIHSTGFYRNKAKHIQEACRRILSHFGGQVPRTMPELLTLP
HHCCCHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
GVARKTANVVLAHAFGINAGVTVDTHVKRLSRRLGLTEHEDPVRIEKDLMQLLPQADWEN
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCC
WSIRLIDHGRAICTARRPLCQQCFLADLCPSAPLLRPAAPDEKMDKKATKASPT
CEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
ASKRALRQRALEILLRLKRHYPNSTCALHYRTPLQLLVATILSAQCTDERVNQVTPELF
CHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHH
RRFPDAQALATAPREEIEALIHSTGFYRNKAKHIQEACRRILSHFGGQVPRTMPELLTLP
HHCCCHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
GVARKTANVVLAHAFGINAGVTVDTHVKRLSRRLGLTEHEDPVRIEKDLMQLLPQADWEN
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCC
WSIRLIDHGRAICTARRPLCQQCFLADLCPSAPLLRPAAPDEKMDKKATKASPT
CEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]