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The map label for this gene is puuB [H]

Identifier: 86608407

GI number: 86608407

Start: 965343

End: 966683

Strand: Reverse

Name: puuB [H]

Synonym: CYB_0927

Alternate gene names: 86608407

Gene position: 966683-965343 (Counterclockwise)

Preceding gene: 86608408

Following gene: 86608406

Centisome position: 31.73

GC content: 63.68

Gene sequence:

>1341_bases
ATGAGAAAGGGCCTTTATCCTGCCAGCACCTACGATCCTGCCTGCCCGGTCGGCAGCTACTGGGACAGCACGATAGCAGA
TCCCGGCTGGCGGCATCCGGCACCTTTGCAGGAGCAGGTGAGCGCTGAGGTGGTCATTGTGGGGGGAGGATTGACGGGGC
TATCGACGGCTCTGCACCTGGCCCGCGACCACGGCATCCATGCTTGTCTGCTGGAGGCGGGATCCCTGGGTTGGGGGGCC
TCGGGACGCAACGGTGGCTTTTGCGGGGTGGGGGCGAGCCACCTTACCCACACCCAGATGGTGCGGCGGGTGGGCCTGGC
AGAGACAGAGCGCTACTACCGGGATCAATGGCAGGCAGTGGAAACCGTTCAGGAGCTGGCGGCCAGCGAGGGCTTCTCCC
TAGAGGCTCAGGGACAGGGCTGCTATGCAGTGGCCCATCGTCCACAGGCATGGTCGGAGTTAGAGGCCGAGTACCAGATC
TGTACTCAGGTGGCGGGCTACCCGGCCCAGCTCCTCAACCCCAAAGAGCTGCGGGAACGCGGCTTCAACAGCCAAGAGAA
CCACGGCGCTTTGCACATCGGGGTGGGGTTTGGCCTCAACCCCCTCAAGCTCACCCAAGGACTGGCCCAAGTGGCCCACC
GGTGGGGGGCAAAACTGTACAGCGGCAGTCCGGTCACGGCCTGGGAAAAGGTGGGATCCCAACACATCTGTCACACGCCG
GGAGGAACGGTGCGCGCTCGGCACTTGGTCATTGCCACCAATGGCTACACCCCAGAGGCCCTGTATCCCCAACTGGCGGG
ATCCCTGTTGCCGGCACTGTCCAACATCCTGGTCACCCGACCTCTAACGCCTCAGGAGCAGAAAGCGCAGGGGTGGTGGG
ATCCGACGCCGCTCTACGACATGCGGCGGCTGCTGTTCTATTACCGTCTGCTGCCGGATGGGCGCCTGCTGTTTGGGGGA
CGGGGTGGCACCCGCGATACCCCGACGGAGCGACAGCAGTTGCGCACTTGGATGACCCGACAGCTCTATCGTCTTTTCCC
TGCTTGGGAAGGGGTGGAGATCGAGTACGCCTGGAATGGACTGGTCTGCCTTTCTCGGCAGTTTCACCCCCATATTGGCC
CTTCGCCGGCGGATCCCAGCCTGTGGTACGGCTTGGCCTATCACGGCAGCGGTGTGGCCACAGCAGTGTGGGCGGGCAAA
ATGCTGGCCCAGGAGATTGCCGGGATCCCTTCTCAGATGAGCGCTCTGTTTCGCCAGCCGCCCCGCGCTTTCCCTTTGCC
CTGGCTACGGCTTCACTATCTGCGGCTGGGGTATCTCTGGTGGCAGTTGACGGATGGATAG

Upstream 100 bases:

>100_bases
GAGTGAGGGGATCCCCTTCAGCACTTGTCCTGCGACCGGTCGCCTCAGCGGTACCGAGCACTTCGGCGTTGCCCAGCAAG
ATGTTCTTCCCATTGCTCGT

Downstream 100 bases:

>100_bases
CCTCTCCAAGTGTGCGGGGGATCCCTCTGTTCGGCGGAGAAGTCCTGCCCTAGTCTGGTCGTAGTTGGGTGGAAACCATC
GCTTGTTGTTGCTCCCGAGG

Product: oxidoreductase, FAD-dependent

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MRKGLYPASTYDPACPVGSYWDSTIADPGWRHPAPLQEQVSAEVVIVGGGLTGLSTALHLARDHGIHACLLEAGSLGWGA
SGRNGGFCGVGASHLTHTQMVRRVGLAETERYYRDQWQAVETVQELAASEGFSLEAQGQGCYAVAHRPQAWSELEAEYQI
CTQVAGYPAQLLNPKELRERGFNSQENHGALHIGVGFGLNPLKLTQGLAQVAHRWGAKLYSGSPVTAWEKVGSQHICHTP
GGTVRARHLVIATNGYTPEALYPQLAGSLLPALSNILVTRPLTPQEQKAQGWWDPTPLYDMRRLLFYYRLLPDGRLLFGG
RGGTRDTPTERQQLRTWMTRQLYRLFPAWEGVEIEYAWNGLVCLSRQFHPHIGPSPADPSLWYGLAYHGSGVATAVWAGK
MLAQEIAGIPSQMSALFRQPPRAFPLPWLRLHYLRLGYLWWQLTDG

Sequences:

>Translated_446_residues
MRKGLYPASTYDPACPVGSYWDSTIADPGWRHPAPLQEQVSAEVVIVGGGLTGLSTALHLARDHGIHACLLEAGSLGWGA
SGRNGGFCGVGASHLTHTQMVRRVGLAETERYYRDQWQAVETVQELAASEGFSLEAQGQGCYAVAHRPQAWSELEAEYQI
CTQVAGYPAQLLNPKELRERGFNSQENHGALHIGVGFGLNPLKLTQGLAQVAHRWGAKLYSGSPVTAWEKVGSQHICHTP
GGTVRARHLVIATNGYTPEALYPQLAGSLLPALSNILVTRPLTPQEQKAQGWWDPTPLYDMRRLLFYYRLLPDGRLLFGG
RGGTRDTPTERQQLRTWMTRQLYRLFPAWEGVEIEYAWNGLVCLSRQFHPHIGPSPADPSLWYGLAYHGSGVATAVWAGK
MLAQEIAGIPSQMSALFRQPPRAFPLPWLRLHYLRLGYLWWQLTDG
>Mature_446_residues
MRKGLYPASTYDPACPVGSYWDSTIADPGWRHPAPLQEQVSAEVVIVGGGLTGLSTALHLARDHGIHACLLEAGSLGWGA
SGRNGGFCGVGASHLTHTQMVRRVGLAETERYYRDQWQAVETVQELAASEGFSLEAQGQGCYAVAHRPQAWSELEAEYQI
CTQVAGYPAQLLNPKELRERGFNSQENHGALHIGVGFGLNPLKLTQGLAQVAHRWGAKLYSGSPVTAWEKVGSQHICHTP
GGTVRARHLVIATNGYTPEALYPQLAGSLLPALSNILVTRPLTPQEQKAQGWWDPTPLYDMRRLLFYYRLLPDGRLLFGG
RGGTRDTPTERQQLRTWMTRQLYRLFPAWEGVEIEYAWNGLVCLSRQFHPHIGPSPADPSLWYGLAYHGSGVATAVWAGK
MLAQEIAGIPSQMSALFRQPPRAFPLPWLRLHYLRLGYLWWQLTDG

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=415, Percent_Identity=29.3975903614458, Blast_Score=146, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 49349; Mature: 49349

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKGLYPASTYDPACPVGSYWDSTIADPGWRHPAPLQEQVSAEVVIVGGGLTGLSTALHL
CCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCHHHCCCCEEEEEECCHHHHHHHHHH
ARDHGIHACLLEAGSLGWGASGRNGGFCGVGASHLTHTQMVRRVGLAETERYYRDQWQAV
HHHCCCEEEEEECCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
ETVQELAASEGFSLEAQGQGCYAVAHRPQAWSELEAEYQICTQVAGYPAQLLNPKELRER
HHHHHHHHCCCCEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHC
GFNSQENHGALHIGVGFGLNPLKLTQGLAQVAHRWGAKLYSGSPVTAWEKVGSQHICHTP
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCEEECCCCCHHHHHCCCCCEEECC
GGTVRARHLVIATNGYTPEALYPQLAGSLLPALSNILVTRPLTPQEQKAQGWWDPTPLYD
CCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCHHHCCCCCCCCCHHH
MRRLLFYYRLLPDGRLLFGGRGGTRDTPTERQQLRTWMTRQLYRLFPAWEGVEIEYAWNG
HHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
LVCLSRQFHPHIGPSPADPSLWYGLAYHGSGVATAVWAGKMLAQEIAGIPSQMSALFRQP
EEEECCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCC
PRAFPLPWLRLHYLRLGYLWWQLTDG
CCCCCCHHHHHHHHHHHHEEEEEECC
>Mature Secondary Structure
MRKGLYPASTYDPACPVGSYWDSTIADPGWRHPAPLQEQVSAEVVIVGGGLTGLSTALHL
CCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCHHHCCCCEEEEEECCHHHHHHHHHH
ARDHGIHACLLEAGSLGWGASGRNGGFCGVGASHLTHTQMVRRVGLAETERYYRDQWQAV
HHHCCCEEEEEECCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
ETVQELAASEGFSLEAQGQGCYAVAHRPQAWSELEAEYQICTQVAGYPAQLLNPKELRER
HHHHHHHHCCCCEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHC
GFNSQENHGALHIGVGFGLNPLKLTQGLAQVAHRWGAKLYSGSPVTAWEKVGSQHICHTP
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCEEECCCCCHHHHHCCCCCEEECC
GGTVRARHLVIATNGYTPEALYPQLAGSLLPALSNILVTRPLTPQEQKAQGWWDPTPLYD
CCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCHHHCCCCCCCCCHHH
MRRLLFYYRLLPDGRLLFGGRGGTRDTPTERQQLRTWMTRQLYRLFPAWEGVEIEYAWNG
HHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
LVCLSRQFHPHIGPSPADPSLWYGLAYHGSGVATAVWAGKMLAQEIAGIPSQMSALFRQP
EEEECCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCC
PRAFPLPWLRLHYLRLGYLWWQLTDG
CCCCCCHHHHHHHHHHHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]