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The map label for this gene is tuf [H]

Identifier: 86608401

GI number: 86608401

Start: 958905

End: 960134

Strand: Direct

Name: tuf [H]

Synonym: CYB_0921

Alternate gene names: 86608401

Gene position: 958905-960134 (Clockwise)

Preceding gene: 86608400

Following gene: 86608402

Centisome position: 31.47

GC content: 58.54

Gene sequence:

>1230_bases
ATGGCACGCGCTAAGTTTGAACGCACCAAACCCCATGTCAACGTTGGCACCATCGGCCATGTTGACCACGGCAAGACCAC
CCTGACGGCTGCCATCACCACCACCTTGGCTGCTTTGGGTCAGGCCACAGCCAAAAGGTACGATGAGATCGACGCAGCGC
CAGAGGAAAAAGCTCGCGGCATTACCATCAACACCGCCCACGTCGAGTATCAGACTGCCAACCGTCACTACGCCCACGTG
GATTGTCCCGGCCACGCCGACTACGTGAAGAACATGATCACAGGCGCGGCTCAGATGGATGGGGCCATCTTGGTGGTGTC
TGCAGCTGATGGTCCCATGCCCCAAACACGGGAGCACATCCTGTTGGCCCGTCAGGTAGGGGTGCCCAGCTTGGTGGTCT
TTCTGAACAAGGCAGACATGGTGGACGATCCAGAGCTGCTGGAGTTGGTGGAGCTGGAAGTGCGGGAGCTTCTCTCCAAA
TACGACTTCCCCGGCGACGACGTTCCCATCATCAGAGGATCGGCGCTGAAAGCCCTAGAAAGAATGACCGCCAACCCCAA
AACCCAGCGGGGCGAAGATCCTTGGGTGGACAAAATTTATGAGCTGATGGATGCGGTGGACTCCTACATTCCCACGCCGG
AGCGGGATGTGGACAAGCCCTTCCTGATGGCTGTGGAGGATGTCTTCTCGATTACTGGTCGGGGCACGGTGGCCACGGGC
CGGATCGAGCGGGGCCGCATCAAAGTGGGCGAAACGGTGGAATTGGTGGGCCTGCGGGAAACTCGCTCCACCACTGTTAC
TGGCCTGGAAATGTTCCAAAAAACCCTCGATGAGGGGATCGCCGGCGACAACGTTGGTGTGCTGTTGCGCGGCATTCAGA
AGAACGAAGTGGAGCGCGGCATGGTGCTGGCCAAGCCCAAGACCATTACTCCCCACACCAACTTCGAGTCTGAGGTGTAT
GTCCTCAAGAAAGAGGAGGGAGGTCGCCACACGCCCTTCTTTGCCGGCTATCGTCCTCAGTTCTATGTGCGCACCACCGA
TGTAACCGGCACCATCTCTTCCTTCACGGCTGATGACGGATCCCAGCCAGAGATGGTGATGCCTGGTGACCGCGTTAAGA
TGACGGTGGAGCTGATCCAGCCCATCGCCATTGAGCAGGGGATGCGCTTTGCTATCCGCGAGGGTGGCCGTACTGTCGGA
GCGGGTGTTGTCTCCAAGATCCTGAAGTAA

Upstream 100 bases:

>100_bases
AGCCACTACGAGGAAGTTCCTCGCAGTATCGCCGAAGCCATCATTGCCAAAAACAAAGGAAATGAGTAGCTGACTGAAAC
CAAAAAGGAAGTGATGATCC

Downstream 100 bases:

>100_bases
GCGGCTTTTTCCTGGCAGGGATTGAGTGCCGACGGCCCCAGACCAACTCAGTTGCGATAGTGGCGCGGAGCACTTTCCCT
GCCAGCTTTTTTGAGTCCCC

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MARAKFERTKPHVNVGTIGHVDHGKTTLTAAITTTLAALGQATAKRYDEIDAAPEEKARGITINTAHVEYQTANRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPSLVVFLNKADMVDDPELLELVELEVRELLSK
YDFPGDDVPIIRGSALKALERMTANPKTQRGEDPWVDKIYELMDAVDSYIPTPERDVDKPFLMAVEDVFSITGRGTVATG
RIERGRIKVGETVELVGLRETRSTTVTGLEMFQKTLDEGIAGDNVGVLLRGIQKNEVERGMVLAKPKTITPHTNFESEVY
VLKKEEGGRHTPFFAGYRPQFYVRTTDVTGTISSFTADDGSQPEMVMPGDRVKMTVELIQPIAIEQGMRFAIREGGRTVG
AGVVSKILK

Sequences:

>Translated_409_residues
MARAKFERTKPHVNVGTIGHVDHGKTTLTAAITTTLAALGQATAKRYDEIDAAPEEKARGITINTAHVEYQTANRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPSLVVFLNKADMVDDPELLELVELEVRELLSK
YDFPGDDVPIIRGSALKALERMTANPKTQRGEDPWVDKIYELMDAVDSYIPTPERDVDKPFLMAVEDVFSITGRGTVATG
RIERGRIKVGETVELVGLRETRSTTVTGLEMFQKTLDEGIAGDNVGVLLRGIQKNEVERGMVLAKPKTITPHTNFESEVY
VLKKEEGGRHTPFFAGYRPQFYVRTTDVTGTISSFTADDGSQPEMVMPGDRVKMTVELIQPIAIEQGMRFAIREGGRTVG
AGVVSKILK
>Mature_408_residues
ARAKFERTKPHVNVGTIGHVDHGKTTLTAAITTTLAALGQATAKRYDEIDAAPEEKARGITINTAHVEYQTANRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPSLVVFLNKADMVDDPELLELVELEVRELLSKY
DFPGDDVPIIRGSALKALERMTANPKTQRGEDPWVDKIYELMDAVDSYIPTPERDVDKPFLMAVEDVFSITGRGTVATGR
IERGRIKVGETVELVGLRETRSTTVTGLEMFQKTLDEGIAGDNVGVLLRGIQKNEVERGMVLAKPKTITPHTNFESEVYV
LKKEEGGRHTPFFAGYRPQFYVRTTDVTGTISSFTADDGSQPEMVMPGDRVKMTVELIQPIAIEQGMRFAIREGGRTVGA
GVVSKILK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=409, Percent_Identity=55.2567237163814, Blast_Score=462, Evalue=1e-130,
Organism=Homo sapiens, GI223555963, Length=439, Percent_Identity=30.2961275626424, Blast_Score=183, Evalue=3e-46,
Organism=Homo sapiens, GI5729864, Length=439, Percent_Identity=30.2961275626424, Blast_Score=182, Evalue=4e-46,
Organism=Homo sapiens, GI4503475, Length=457, Percent_Identity=28.4463894967177, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI4503471, Length=456, Percent_Identity=28.2894736842105, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI194018522, Length=444, Percent_Identity=27.4774774774775, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI46094014, Length=442, Percent_Identity=28.2805429864253, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI194097354, Length=444, Percent_Identity=27.4774774774775, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI194018520, Length=444, Percent_Identity=27.4774774774775, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI54607086, Length=353, Percent_Identity=28.8951841359773, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI4503507, Length=277, Percent_Identity=27.7978339350181, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI94966754, Length=132, Percent_Identity=32.5757575757576, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI310132016, Length=109, Percent_Identity=33.9449541284404, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI310110807, Length=109, Percent_Identity=33.9449541284404, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI310123363, Length=109, Percent_Identity=33.9449541284404, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI157426893, Length=368, Percent_Identity=22.8260869565217, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1790412, Length=408, Percent_Identity=73.5294117647059, Blast_Score=581, Evalue=1e-167,
Organism=Escherichia coli, GI1789737, Length=408, Percent_Identity=73.5294117647059, Blast_Score=580, Evalue=1e-167,
Organism=Escherichia coli, GI2367247, Length=342, Percent_Identity=30.7017543859649, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI48994988, Length=284, Percent_Identity=30.6338028169014, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1789108, Length=322, Percent_Identity=27.639751552795, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788922, Length=140, Percent_Identity=32.8571428571429, Blast_Score=62, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=415, Percent_Identity=49.1566265060241, Blast_Score=385, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI25141371, Length=400, Percent_Identity=44.5, Blast_Score=337, Evalue=6e-93,
Organism=Caenorhabditis elegans, GI17552884, Length=450, Percent_Identity=29.1111111111111, Blast_Score=171, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI17569207, Length=450, Percent_Identity=29.1111111111111, Blast_Score=171, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI32566629, Length=456, Percent_Identity=27.1929824561404, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI32566303, Length=438, Percent_Identity=27.6255707762557, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI115532067, Length=446, Percent_Identity=26.0089686098655, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI115532065, Length=443, Percent_Identity=25.9593679458239, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17506081, Length=284, Percent_Identity=30.9859154929577, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=36.0544217687075, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17557151, Length=302, Percent_Identity=25.1655629139073, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=409, Percent_Identity=62.8361858190709, Blast_Score=523, Evalue=1e-149,
Organism=Saccharomyces cerevisiae, GI6325337, Length=448, Percent_Identity=30.1339285714286, Blast_Score=173, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6319594, Length=448, Percent_Identity=30.1339285714286, Blast_Score=173, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6320377, Length=448, Percent_Identity=25.6696428571429, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6322937, Length=463, Percent_Identity=23.110151187905, Blast_Score=102, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6320863, Length=281, Percent_Identity=26.3345195729537, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323320, Length=385, Percent_Identity=24.9350649350649, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=171, Percent_Identity=32.1637426900585, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI281363316, Length=405, Percent_Identity=54.5679012345679, Blast_Score=457, Evalue=1e-129,
Organism=Drosophila melanogaster, GI17864358, Length=405, Percent_Identity=54.5679012345679, Blast_Score=457, Evalue=1e-129,
Organism=Drosophila melanogaster, GI19921738, Length=401, Percent_Identity=48.6284289276808, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI45553807, Length=444, Percent_Identity=29.0540540540541, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI45553816, Length=444, Percent_Identity=29.0540540540541, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24651721, Length=444, Percent_Identity=29.0540540540541, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI17864154, Length=444, Percent_Identity=29.0540540540541, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24652838, Length=444, Percent_Identity=29.7297297297297, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI17137572, Length=444, Percent_Identity=29.7297297297297, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI45550900, Length=452, Percent_Identity=28.0973451327434, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI17137380, Length=444, Percent_Identity=27.027027027027, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI19922690, Length=313, Percent_Identity=30.0319488817891, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24647046, Length=277, Percent_Identity=27.7978339350181, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24647048, Length=277, Percent_Identity=27.7978339350181, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI28572034, Length=166, Percent_Identity=36.7469879518072, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28574573, Length=146, Percent_Identity=32.8767123287671, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 44856; Mature: 44725

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARAKFERTKPHVNVGTIGHVDHGKTTLTAAITTTLAALGQATAKRYDEIDAAPEEKARG
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCC
ITINTAHVEYQTANRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPSLVVFLNKADMVDDPELLELVELEVRELLSKYDFPGDDVPIIRGSALKALE
HHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCHHHHHHH
RMTANPKTQRGEDPWVDKIYELMDAVDSYIPTPERDVDKPFLMAVEDVFSITGRGTVATG
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEC
RIERGRIKVGETVELVGLRETRSTTVTGLEMFQKTLDEGIAGDNVGVLLRGIQKNEVERG
CEECCEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHEEECCHHHHHHCC
MVLAKPKTITPHTNFESEVYVLKKEEGGRHTPFFAGYRPQFYVRTTDVTGTISSFTADDG
EEEECCCEECCCCCCCCEEEEEEECCCCCCCCEECCCCCEEEEEEECCCCCHHCCCCCCC
SQPEMVMPGDRVKMTVELIQPIAIEQGMRFAIREGGRTVGAGVVSKILK
CCCCEECCCCCCEEEHHHHHHHHHHCCCEEEHHCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
ARAKFERTKPHVNVGTIGHVDHGKTTLTAAITTTLAALGQATAKRYDEIDAAPEEKARG
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCC
ITINTAHVEYQTANRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPSLVVFLNKADMVDDPELLELVELEVRELLSKYDFPGDDVPIIRGSALKALE
HHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCHHHHHHH
RMTANPKTQRGEDPWVDKIYELMDAVDSYIPTPERDVDKPFLMAVEDVFSITGRGTVATG
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEC
RIERGRIKVGETVELVGLRETRSTTVTGLEMFQKTLDEGIAGDNVGVLLRGIQKNEVERG
CEECCEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHEEECCHHHHHHCC
MVLAKPKTITPHTNFESEVYVLKKEEGGRHTPFFAGYRPQFYVRTTDVTGTISSFTADDG
EEEECCCEECCCCCCCCEEEEEEECCCCCCCCEECCCCCEEEEEEECCCCCHHCCCCCCC
SQPEMVMPGDRVKMTVELIQPIAIEQGMRFAIREGGRTVGAGVVSKILK
CCCCEECCCCCCEEEHHHHHHHHHHCCCEEEHHCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA