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The map label for this gene is gspO [H]

Identifier: 86608389

GI number: 86608389

Start: 944225

End: 945076

Strand: Reverse

Name: gspO [H]

Synonym: CYB_0909

Alternate gene names: 86608389

Gene position: 945076-944225 (Counterclockwise)

Preceding gene: 86608390

Following gene: 86608387

Centisome position: 31.02

GC content: 63.85

Gene sequence:

>852_bases
ATGTGGCTGAGCAGCGCCGACGCCAACAGAATCTGGGAGCTGCGCCTGTTGGCCTGGGTGCTGGTACTGGTGCTGGGGGC
CTGTGTGGGCAGCTTCTTGAACGTGGTGGTCTATCGCCTGCCGCAGGGTCTTTCTCTGCTCCATCCCGGATCCCATTGTC
CAAGCTGCAAGACGCCGCTGGGCCCCACGGAGAACATTCCCATCCTAGGCTGGTTGTTGCTGCGCGGTCGCTGCCGCCAT
TGTGGAGCGCAGATTAGCTGGCGCTACCCTGCCGTTGAGGCGTTGACAATGGGCCTGTTTGCCCTCAGCATCGGCGCTTT
GGGGTTTTCTGCCCAAGGGATCCTCACCTGCATCTTGTTGAGCTGGCTGTTGGCCCTGGCGCTCATCGATCTGGATACCT
TTTTGCTGCCGGAGGAGCTGACCCGTTCAGGCCTGGTGGTGGGGCTTTTGGCCCGCCTGGCTCTGCCGTGGCTCCAGGGA
TCCGGATCCCTGGCCGCTACAGGCCTGTCACTGGTGGAAGGGATTGCAGGAGCTGTGCTGGGCCTCTGGCTGCTGGAGGG
CATTGGCCTCTTGGCCCGTTGGGTCTTGGGGCGGGAGGCTATGGGCGGGGGAGATGGCAAGCTGTTGGCCCTGATCGGCA
TGTGGTTGGGCTGGCAGGGGGTGGTGGTGGCCCTTCTTTTTGGTTCCGGGTTTGGCCTGCTGGCTGGCTTGCTGGCCATG
ACCAAGGGGCGGGCCCGCTTGGGAAAACCCATTCCCTTTGGCCCCTACCTGGCCTTGGGTGGAGGGGTAGCCGCTCTAGC
CGGATCCCAACTGGTGGAGGGCTACCTGCGCTGGCTTGACCTGGTGGGGTAG

Upstream 100 bases:

>100_bases
TCTGCCTTTAGGGACTCCGTCCTGCCGACGCGATGGGAATGGCTGCCATGCTCGAGATTCTCCCCTACTGCACAACGCTC
ACATGATGTGGCTGAGCATG

Downstream 100 bases:

>100_bases
AGCTACCCTGCCCCCCTCCCCAGGAGAGAGAGGGGTAGAGATGCCCGTTCGGGCCAACTTAGGACAGGGAGAACTCTCTA
CTCCACCCCCTCGATCCGGT

Product: prepilin peptidase

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MWLSSADANRIWELRLLAWVLVLVLGACVGSFLNVVVYRLPQGLSLLHPGSHCPSCKTPLGPTENIPILGWLLLRGRCRH
CGAQISWRYPAVEALTMGLFALSIGALGFSAQGILTCILLSWLLALALIDLDTFLLPEELTRSGLVVGLLARLALPWLQG
SGSLAATGLSLVEGIAGAVLGLWLLEGIGLLARWVLGREAMGGGDGKLLALIGMWLGWQGVVVALLFGSGFGLLAGLLAM
TKGRARLGKPIPFGPYLALGGGVAALAGSQLVEGYLRWLDLVG

Sequences:

>Translated_283_residues
MWLSSADANRIWELRLLAWVLVLVLGACVGSFLNVVVYRLPQGLSLLHPGSHCPSCKTPLGPTENIPILGWLLLRGRCRH
CGAQISWRYPAVEALTMGLFALSIGALGFSAQGILTCILLSWLLALALIDLDTFLLPEELTRSGLVVGLLARLALPWLQG
SGSLAATGLSLVEGIAGAVLGLWLLEGIGLLARWVLGREAMGGGDGKLLALIGMWLGWQGVVVALLFGSGFGLLAGLLAM
TKGRARLGKPIPFGPYLALGGGVAALAGSQLVEGYLRWLDLVG
>Mature_283_residues
MWLSSADANRIWELRLLAWVLVLVLGACVGSFLNVVVYRLPQGLSLLHPGSHCPSCKTPLGPTENIPILGWLLLRGRCRH
CGAQISWRYPAVEALTMGLFALSIGALGFSAQGILTCILLSWLLALALIDLDTFLLPEELTRSGLVVGLLARLALPWLQG
SGSLAATGLSLVEGIAGAVLGLWLLEGIGLLARWVLGREAMGGGDGKLLALIGMWLGWQGVVVALLFGSGFGLLAGLLAM
TKGRARLGKPIPFGPYLALGGGVAALAGSQLVEGYLRWLDLVG

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=260, Percent_Identity=36.5384615384615, Blast_Score=125, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 29762; Mature: 29762

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWLSSADANRIWELRLLAWVLVLVLGACVGSFLNVVVYRLPQGLSLLHPGSHCPSCKTPL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCC
GPTENIPILGWLLLRGRCRHCGAQISWRYPAVEALTMGLFALSIGALGFSAQGILTCILL
CCCCCCHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
SWLLALALIDLDTFLLPEELTRSGLVVGLLARLALPWLQGSGSLAATGLSLVEGIAGAVL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
GLWLLEGIGLLARWVLGREAMGGGDGKLLALIGMWLGWQGVVVALLFGSGFGLLAGLLAM
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
TKGRARLGKPIPFGPYLALGGGVAALAGSQLVEGYLRWLDLVG
HCCHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MWLSSADANRIWELRLLAWVLVLVLGACVGSFLNVVVYRLPQGLSLLHPGSHCPSCKTPL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCC
GPTENIPILGWLLLRGRCRHCGAQISWRYPAVEALTMGLFALSIGALGFSAQGILTCILL
CCCCCCHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
SWLLALALIDLDTFLLPEELTRSGLVVGLLARLALPWLQGSGSLAATGLSLVEGIAGAVL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
GLWLLEGIGLLARWVLGREAMGGGDGKLLALIGMWLGWQGVVVALLFGSGFGLLAGLLAM
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
TKGRARLGKPIPFGPYLALGGGVAALAGSQLVEGYLRWLDLVG
HCCHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]