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The map label for this gene is purN [H]

Identifier: 86608381

GI number: 86608381

Start: 933678

End: 934340

Strand: Reverse

Name: purN [H]

Synonym: CYB_0901

Alternate gene names: 86608381

Gene position: 934340-933678 (Counterclockwise)

Preceding gene: 86608382

Following gene: 86608380

Centisome position: 30.67

GC content: 61.09

Gene sequence:

>663_bases
ATGAACTCGCCCAGCTTACTCTGGCCGGATCCCGACGGGATCCCAGTGCCCTCTCAACCGGGGATCCTTGGCATTTTGGC
TTCCGGCAATGGCAGCAATTTCGAAGCTATTGCCCAAGCCATTGAGGCTGGGGAACTGCAGGCCCAGATTGCTGTGGTCA
TCACCAACAACCCCAAAGCCTACGTGCGGCAGCGGGCCCAAAAGCGAGGGATCCCTTGCGTCTTGCTCGACCACCGAGAC
TACCCCTGTCGCGAAGACCTGGATGCAGCTATCCTCCAAGTGCTCTGGCAGCATCACGTGGAATGGGTGATCATGGCCGG
GTGGATGCGCCTGGTTACCCAGGTGTTGTTGTCCGCCTACCCGGATCGCGTCCTGAACCTGCATCCTAGCCTGCTGCCCA
GCTTCAAGGGCCTGCGGGCTGTTGAACAAGCCCTGAAGTGCGGTGTGAAAATCGCCGGCTGCACCGTTCATCGGGTCACC
CTGGAAATGGACAGTGGCCCCATCGTGGCCCAAGCAGCCGTGCCGGTTCTGCCCGAAGATACCGTGGAATCCCTTTACCG
GCGCATCCAGGCCCAGGAGCACCGCCTCTACCCGCTGGCCATCCGCCTCTGCCTGGCAGAGGGATCCCTGCTACCCAAGT
CTTCGGGGAAGCTGCACAATTGA

Upstream 100 bases:

>100_bases
CAAGTTTTTGGATCCCTCTCTGGATCAACCTGGCCAGAACCAGAGGGGAGGCTGTCTGGGTTTAAAATAGCGGGGCTGCA
GACTTTCCCACCAAGCGCTC

Downstream 100 bases:

>100_bases
GGTTAAGTCCAACGCTGGCGCATCCCATGGCCGAGATCCCGCCTCTCCTCGTCTTCGATAGCCCGGTGATCCTGGCCGGT
CGTCTGGCTCTATGGCAAGA

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MNSPSLLWPDPDGIPVPSQPGILGILASGNGSNFEAIAQAIEAGELQAQIAVVITNNPKAYVRQRAQKRGIPCVLLDHRD
YPCREDLDAAILQVLWQHHVEWVIMAGWMRLVTQVLLSAYPDRVLNLHPSLLPSFKGLRAVEQALKCGVKIAGCTVHRVT
LEMDSGPIVAQAAVPVLPEDTVESLYRRIQAQEHRLYPLAIRLCLAEGSLLPKSSGKLHN

Sequences:

>Translated_220_residues
MNSPSLLWPDPDGIPVPSQPGILGILASGNGSNFEAIAQAIEAGELQAQIAVVITNNPKAYVRQRAQKRGIPCVLLDHRD
YPCREDLDAAILQVLWQHHVEWVIMAGWMRLVTQVLLSAYPDRVLNLHPSLLPSFKGLRAVEQALKCGVKIAGCTVHRVT
LEMDSGPIVAQAAVPVLPEDTVESLYRRIQAQEHRLYPLAIRLCLAEGSLLPKSSGKLHN
>Mature_220_residues
MNSPSLLWPDPDGIPVPSQPGILGILASGNGSNFEAIAQAIEAGELQAQIAVVITNNPKAYVRQRAQKRGIPCVLLDHRD
YPCREDLDAAILQVLWQHHVEWVIMAGWMRLVTQVLLSAYPDRVLNLHPSLLPSFKGLRAVEQALKCGVKIAGCTVHRVT
LEMDSGPIVAQAAVPVLPEDTVESLYRRIQAQEHRLYPLAIRLCLAEGSLLPKSSGKLHN

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=196, Percent_Identity=39.2857142857143, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI209869995, Length=196, Percent_Identity=39.2857142857143, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI209869993, Length=196, Percent_Identity=39.2857142857143, Blast_Score=152, Evalue=3e-37,
Organism=Escherichia coli, GI1788846, Length=186, Percent_Identity=37.6344086021505, Blast_Score=136, Evalue=8e-34,
Organism=Escherichia coli, GI1787483, Length=164, Percent_Identity=31.0975609756098, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17567511, Length=179, Percent_Identity=36.3128491620112, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6320616, Length=193, Percent_Identity=31.0880829015544, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24582400, Length=191, Percent_Identity=38.7434554973822, Blast_Score=140, Evalue=8e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 24122; Mature: 24122

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSPSLLWPDPDGIPVPSQPGILGILASGNGSNFEAIAQAIEAGELQAQIAVVITNNPKA
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEEEECCCHH
YVRQRAQKRGIPCVLLDHRDYPCREDLDAAILQVLWQHHVEWVIMAGWMRLVTQVLLSAY
HHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PDRVLNLHPSLLPSFKGLRAVEQALKCGVKIAGCTVHRVTLEMDSGPIVAQAAVPVLPED
CHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEECEEEEEEEEEECCCCEEEECCCCCCCHH
TVESLYRRIQAQEHRLYPLAIRLCLAEGSLLPKSSGKLHN
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MNSPSLLWPDPDGIPVPSQPGILGILASGNGSNFEAIAQAIEAGELQAQIAVVITNNPKA
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEEEECCCHH
YVRQRAQKRGIPCVLLDHRDYPCREDLDAAILQVLWQHHVEWVIMAGWMRLVTQVLLSAY
HHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PDRVLNLHPSLLPSFKGLRAVEQALKCGVKIAGCTVHRVTLEMDSGPIVAQAAVPVLPED
CHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEECEEEEEEEEEECCCCEEEECCCCCCCHH
TVESLYRRIQAQEHRLYPLAIRLCLAEGSLLPKSSGKLHN
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]