Click here to switch to the map view.

The map label for this gene is rutB [H]

Identifier: 86608280

GI number: 86608280

Start: 821600

End: 822274

Strand: Direct

Name: rutB [H]

Synonym: CYB_0796

Alternate gene names: 86608280

Gene position: 821600-822274 (Clockwise)

Preceding gene: 86608279

Following gene: 86608281

Centisome position: 26.97

GC content: 57.78

Gene sequence:

>675_bases
ATGTTTCGGATCCCGGCCTTGCCCTACGAGTATGAAGTTCCCAGCCTGTCTCAGTTGGCCCTGGTGATCATCGATATGCA
GCGAGATTTCCTGGAACCAGGCGGCTTTGGGGAGATGCTGGGCAACGATGTCACCCAACTGGGATCCATTGTGCCCACCC
TGAAGGGGCTGCTGGACTTTTTTCGTCAAAAGGGCTTAACCGTCATTCATACCCTGGAGGGTCACCAGCCAGATCTCTCC
GATTGCCCGCCCAGCAAGCGCAAACGCGGCAAGGGATCCCTGACCATTGGGGACGAAGGCCCTATGGGGCGGATTTTGAT
TCGGGGAGAGCCGGGCAACACCATCATCCCTGAGCTTGCTCCGCTTGCGGGAGAGATCGTCATTCCCAAACCCGGCAAGG
GGGCTTTCTACGCCACCGAGCTGCAAGCAATCTTGCAAAAGCGCGGCATCACCCACCTGCTGTTCACCGGGGTAACCACA
GAAGTCTGCGTGCAAACCACAATGCGCGAGGCCAATGACCGCGGCTATGAGTGCCTGCTGGTGGAAGATTGCACCGCCAG
CTACTTCCCAGAGTTCAAGCAAGCCACCCTGGAGATGATCCGGGCGCAAGGGGGCATCGTCGGCTGGACGAGTTCTGCTC
AGAACATCCAAAAAGCGTTATCGGGTTTACAGTGA

Upstream 100 bases:

>100_bases
TTCCCTGACTGGCGGAGAATACTTGGCCCAGCGGTTGAGGGAAATTGGCCTGGCCGAGGCAGGATCCCCGCCCCAGCATT
GATCCCAACTGGGGGTCTCT

Downstream 100 bases:

>100_bases
GTGTGAATAACCCAACAAGCGTGAGGCTGAACCGATGACACTGGCTCAGGAGGCAACTGCCCAAGTCCCCGCCTTTTGCG
AAGGGATCCAGTACTTCGGG

Product: isochorismatase family protein

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase [H]

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MFRIPALPYEYEVPSLSQLALVIIDMQRDFLEPGGFGEMLGNDVTQLGSIVPTLKGLLDFFRQKGLTVIHTLEGHQPDLS
DCPPSKRKRGKGSLTIGDEGPMGRILIRGEPGNTIIPELAPLAGEIVIPKPGKGAFYATELQAILQKRGITHLLFTGVTT
EVCVQTTMREANDRGYECLLVEDCTASYFPEFKQATLEMIRAQGGIVGWTSSAQNIQKALSGLQ

Sequences:

>Translated_224_residues
MFRIPALPYEYEVPSLSQLALVIIDMQRDFLEPGGFGEMLGNDVTQLGSIVPTLKGLLDFFRQKGLTVIHTLEGHQPDLS
DCPPSKRKRGKGSLTIGDEGPMGRILIRGEPGNTIIPELAPLAGEIVIPKPGKGAFYATELQAILQKRGITHLLFTGVTT
EVCVQTTMREANDRGYECLLVEDCTASYFPEFKQATLEMIRAQGGIVGWTSSAQNIQKALSGLQ
>Mature_224_residues
MFRIPALPYEYEVPSLSQLALVIIDMQRDFLEPGGFGEMLGNDVTQLGSIVPTLKGLLDFFRQKGLTVIHTLEGHQPDLS
DCPPSKRKRGKGSLTIGDEGPMGRILIRGEPGNTIIPELAPLAGEIVIPKPGKGAFYATELQAILQKRGITHLLFTGVTT
EVCVQTTMREANDRGYECLLVEDCTASYFPEFKQATLEMIRAQGGIVGWTSSAQNIQKALSGLQ

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081820, Length=209, Percent_Identity=32.0574162679426, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019916
- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: NA

Molecular weight: Translated: 24432; Mature: 24432

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRIPALPYEYEVPSLSQLALVIIDMQRDFLEPGGFGEMLGNDVTQLGSIVPTLKGLLDF
CCCCCCCCCCCCCCCHHHHHHHEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FRQKGLTVIHTLEGHQPDLSDCPPSKRKRGKGSLTIGDEGPMGRILIRGEPGNTIIPELA
HHHCCCEEEEECCCCCCCCCCCCCHHHHCCCCCEEECCCCCCEEEEEEECCCCCCCCCHH
PLAGEIVIPKPGKGAFYATELQAILQKRGITHLLFTGVTTEVCVQTTMREANDRGYECLL
CCCCEEEECCCCCCCCHHHHHHHHHHHCCCCEEEHHCCHHHHHHHHHHHHHCCCCCEEEE
VEDCTASYFPEFKQATLEMIRAQGGIVGWTSSAQNIQKALSGLQ
EECCCHHHCHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCC
>Mature Secondary Structure
MFRIPALPYEYEVPSLSQLALVIIDMQRDFLEPGGFGEMLGNDVTQLGSIVPTLKGLLDF
CCCCCCCCCCCCCCCHHHHHHHEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FRQKGLTVIHTLEGHQPDLSDCPPSKRKRGKGSLTIGDEGPMGRILIRGEPGNTIIPELA
HHHCCCEEEEECCCCCCCCCCCCCHHHHCCCCCEEECCCCCCEEEEEEECCCCCCCCCHH
PLAGEIVIPKPGKGAFYATELQAILQKRGITHLLFTGVTTEVCVQTTMREANDRGYECLL
CCCCEEEECCCCCCCCHHHHHHHHHHHCCCCEEEHHCCHHHHHHHHHHHHHCCCCCEEEE
VEDCTASYFPEFKQATLEMIRAQGGIVGWTSSAQNIQKALSGLQ
EECCCHHHCHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA