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The map label for this gene is livK [C]

Identifier: 86608256

GI number: 86608256

Start: 794683

End: 795936

Strand: Reverse

Name: livK [C]

Synonym: CYB_0772

Alternate gene names: 86608256

Gene position: 795936-794683 (Counterclockwise)

Preceding gene: 86608257

Following gene: 86608255

Centisome position: 26.12

GC content: 59.89

Gene sequence:

>1254_bases
ATGTCTCCCATGAACAAACTCACCCGACGGGCCTTTGCGGCGGCGGCTGCCCTGACGGCTGCTGCTCTTGCCACCACTCT
GCTGCCCAGCACGGCTCAGCAGGCAACTCAAGGGCCGGTCAAAATCGGGGCTTTAATGTCTCTCACCGGGGATCTGCAGG
CCTTCGGGGAAAACTGCCTCAAGGGGATCAAGCTGGCGATTGACCAAATCAACGCGGCTGGCGGCATTTTGGGATCCCCT
GCCCAAGTGGTCGAAGCGGATGACCAGACGTCGGCTCAGCCAGCTATCGACGCAGCTCAACGGTTGGTCACCGTGGAGAA
GGTGGCCGGCATCATCGGCGCCTTGGGCAGTGGCATCACCATTCCGGTGGCCACGACGGTTTCTGTTCCCAATCGGATCC
CGCAAATTTCCCCCGCTTCCACCGCCCCCACCATCACCACCCTGGAGGACAACGATTTTCTGTTTCGCACAGTTCCTTCC
GACGCCTACCAAGGGGTGGCCTTGGCCCAGATCACCCGTGAAGAGAAGCTGGAGCGCCTGGCGATTATTTACATTAACAA
CGACTACGGCCAGGGCCTGGCCGACAGCTTTAAGACGGCTTTTGAAAAGCGGGGCGGCACCATCACCAAGTCGGTTCCCT
ACGAGCCCGGCAAGGCTTCCTACCGCAGTGAGCTGACCCAACTGAAGGAAGGGGATCCCCAGGCGTTGGTGCTGATTGGC
TACCCCGAAAACGGTGTGGTCATTTTGCGGCAAGCTTTGGAAGAAGGACTGTTTGACCGCTTTGTCTTCACCGATGGCAT
GCGCTCTCCCGACGTGGCAGCCCAGGTGGGAGCCGAAAAGCTAGAGGGATCCATCGGCGTTTCGCCTCAGGCCCTCACCG
ATAGCAAGGCTTACCAAATCTTTGCCGAAGCCTACAAAGCCGCCTACGGGGAGCTGCCCCCCACCCCCTACATCGACACC
GCCTACGATGCCGCTTTTGTCCTGGCTCTGGCCATTCAGAAAGCCGGCAGCACCGATGGCACTGCTGTTCGGGATGCTTT
GCGGGAAGTGGCCAATCCCCCCGGCACCGAGATCCTGCCCGGAGAGTGGGCCAAGGCGGTGGAGCTGATCGGCAAAGGGG
AAGACATCTTCTACACCGGCGCCTCTGGATCCATCAACTTCGATGAGAATGGGGAAGTTGCCGGAACCTTTGCCCACTGG
ACTTTCCAAGGGGGGAAGGTGGTGGATCTGAAGGTGTTTGAGCCGGAGAGCTAA

Upstream 100 bases:

>100_bases
GCTTTGAGGTGAGTCAACGTCTGACTGCTACGGTTCAACACTTGCTTGACTTTTGGGATCTGAGTTCTACTTTCCCTTCC
CTCACAGGAGGTTGTTGGTT

Downstream 100 bases:

>100_bases
TTTTCCCCTACGGCTCCTGAGCGGGGGCCGGTTTCTCCTTCCGAGCATGCCTCTAACCCCCAAAACCCTGAACTTTGCTG
AATTTACTGAGCTTTAGGCG

Product: hydrophobic amino acid ABC transporter periplasmic amino acid-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MSPMNKLTRRAFAAAAALTAAALATTLLPSTAQQATQGPVKIGALMSLTGDLQAFGENCLKGIKLAIDQINAAGGILGSP
AQVVEADDQTSAQPAIDAAQRLVTVEKVAGIIGALGSGITIPVATTVSVPNRIPQISPASTAPTITTLEDNDFLFRTVPS
DAYQGVALAQITREEKLERLAIIYINNDYGQGLADSFKTAFEKRGGTITKSVPYEPGKASYRSELTQLKEGDPQALVLIG
YPENGVVILRQALEEGLFDRFVFTDGMRSPDVAAQVGAEKLEGSIGVSPQALTDSKAYQIFAEAYKAAYGELPPTPYIDT
AYDAAFVLALAIQKAGSTDGTAVRDALREVANPPGTEILPGEWAKAVELIGKGEDIFYTGASGSINFDENGEVAGTFAHW
TFQGGKVVDLKVFEPES

Sequences:

>Translated_417_residues
MSPMNKLTRRAFAAAAALTAAALATTLLPSTAQQATQGPVKIGALMSLTGDLQAFGENCLKGIKLAIDQINAAGGILGSP
AQVVEADDQTSAQPAIDAAQRLVTVEKVAGIIGALGSGITIPVATTVSVPNRIPQISPASTAPTITTLEDNDFLFRTVPS
DAYQGVALAQITREEKLERLAIIYINNDYGQGLADSFKTAFEKRGGTITKSVPYEPGKASYRSELTQLKEGDPQALVLIG
YPENGVVILRQALEEGLFDRFVFTDGMRSPDVAAQVGAEKLEGSIGVSPQALTDSKAYQIFAEAYKAAYGELPPTPYIDT
AYDAAFVLALAIQKAGSTDGTAVRDALREVANPPGTEILPGEWAKAVELIGKGEDIFYTGASGSINFDENGEVAGTFAHW
TFQGGKVVDLKVFEPES
>Mature_416_residues
SPMNKLTRRAFAAAAALTAAALATTLLPSTAQQATQGPVKIGALMSLTGDLQAFGENCLKGIKLAIDQINAAGGILGSPA
QVVEADDQTSAQPAIDAAQRLVTVEKVAGIIGALGSGITIPVATTVSVPNRIPQISPASTAPTITTLEDNDFLFRTVPSD
AYQGVALAQITREEKLERLAIIYINNDYGQGLADSFKTAFEKRGGTITKSVPYEPGKASYRSELTQLKEGDPQALVLIGY
PENGVVILRQALEEGLFDRFVFTDGMRSPDVAAQVGAEKLEGSIGVSPQALTDSKAYQIFAEAYKAAYGELPPTPYIDTA
YDAAFVLALAIQKAGSTDGTAVRDALREVANPPGTEILPGEWAKAVELIGKGEDIFYTGASGSINFDENGEVAGTFAHWT
FQGGKVVDLKVFEPES

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Homo sapiens, GI4504141, Length=152, Percent_Identity=34.8684210526316, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1789867, Length=380, Percent_Identity=27.8947368421053, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI48994941, Length=366, Percent_Identity=26.775956284153, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI193211278, Length=123, Percent_Identity=30.0813008130081, Blast_Score=65, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17569063, Length=123, Percent_Identity=30.0813008130081, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 43832; Mature: 43701

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPMNKLTRRAFAAAAALTAAALATTLLPSTAQQATQGPVKIGALMSLTGDLQAFGENCL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHH
KGIKLAIDQINAAGGILGSPAQVVEADDQTSAQPAIDAAQRLVTVEKVAGIIGALGSGIT
HHHHHHHHHHHHCCCCCCCCHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCE
IPVATTVSVPNRIPQISPASTAPTITTLEDNDFLFRTVPSDAYQGVALAQITREEKLERL
EEEEEEECCCCCCCCCCCCCCCCEEEEECCCCEEEEECCCHHHCCHHHHHHHHHHHCCEE
AIIYINNDYGQGLADSFKTAFEKRGGTITKSVPYEPGKASYRSELTQLKEGDPQALVLIG
EEEEEECCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEE
YPENGVVILRQALEEGLFDRFVFTDGMRSPDVAAQVGAEKLEGSIGVSPQALTDSKAYQI
ECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCHHHHCCCCCCCCCHHCCHHHHHH
FAEAYKAAYGELPPTPYIDTAYDAAFVLALAIQKAGSTDGTAVRDALREVANPPGTEILP
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCC
GEWAKAVELIGKGEDIFYTGASGSINFDENGEVAGTFAHWTFQGGKVVDLKVFEPES
CHHHHHHHHHCCCCEEEEECCCCEECCCCCCCEEEEEEEEEECCCEEEEEEEECCCC
>Mature Secondary Structure 
SPMNKLTRRAFAAAAALTAAALATTLLPSTAQQATQGPVKIGALMSLTGDLQAFGENCL
CHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHH
KGIKLAIDQINAAGGILGSPAQVVEADDQTSAQPAIDAAQRLVTVEKVAGIIGALGSGIT
HHHHHHHHHHHHCCCCCCCCHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCE
IPVATTVSVPNRIPQISPASTAPTITTLEDNDFLFRTVPSDAYQGVALAQITREEKLERL
EEEEEEECCCCCCCCCCCCCCCCEEEEECCCCEEEEECCCHHHCCHHHHHHHHHHHCCEE
AIIYINNDYGQGLADSFKTAFEKRGGTITKSVPYEPGKASYRSELTQLKEGDPQALVLIG
EEEEEECCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEE
YPENGVVILRQALEEGLFDRFVFTDGMRSPDVAAQVGAEKLEGSIGVSPQALTDSKAYQI
ECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCHHHHCCCCCCCCCHHCCHHHHHH
FAEAYKAAYGELPPTPYIDTAYDAAFVLALAIQKAGSTDGTAVRDALREVANPPGTEILP
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCC
GEWAKAVELIGKGEDIFYTGASGSINFDENGEVAGTFAHWTFQGGKVVDLKVFEPES
CHHHHHHHHHCCCCEEEEECCCCEECCCCCCCEEEEEEEEEECCCEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]