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The map label for this gene is maeB [C]

Identifier: 86608102

GI number: 86608102

Start: 623105

End: 624499

Strand: Direct

Name: maeB [C]

Synonym: CYB_0613

Alternate gene names: 86608102

Gene position: 623105-624499 (Clockwise)

Preceding gene: 86608100

Following gene: 86608105

Centisome position: 20.45

GC content: 62.72

Gene sequence:

>1395_bases
ATGACAAAACTTACCCCCAGCTCTAGCTATAGCATTGCCCTGCGCATTCGCCTGCTCAACCAGCCGGGAGCCCTAGCCCG
CGTCACCCAGGCCATTGCGGAAGTGGGAGGCAGCCTCAGCCAGATCGATCTCATCCACCAATCGGCAGCAGAAACGGTGC
GAGAAATCTGTGTAGATGCCGTCAGCAGCGAGCATGCTCAGCAAATTTACACCGCCGTTGCCTCCTTGGCCAATGTGAAG
GTGGAGCGGGTGGTGGACCGCACCTTTGAGCTGCACCGCGGGGGAAAGCTGCAGGTGGTGGCCAAAAGCCCCCTGGAGAG
CGCCGCCGATCTGGCCATGGCCTACACGCCGGGGGTGGGGCGGGTTTCCCTAGAAATTGCTGCCAACCCCGCCGCGGTCT
ATGAGTACACCATCAAGGGCAACAGTGTAGGCATTGTAACCGATGGCAGTGCGGTGCTGGGCTTGGGGAATGTCGGCCCG
GAGGCAGCTTTGCCGGTGATGGAGGGGAAGGCGGCGCTGTTCAAGCGCTTTGCCGATATCGATGCCTTTCCTCTGTGCCT
GGCCACTCAGGAGGTGGGGGAAATTGTAGAGACGGTGGTGCGCCTGGCGCCGGGATTTGGCGGCTTTAACCTGGAAGACA
TCAGCGCCCCTCGCTGCTTTGCCATTGAGTCTCAGCTCAAGGCCCGCCTCAACCTGCCGGTGTTTCACGACGATCAGCAC
GGCACCGCCATTGTGGTGCTGGCGGCGCTGCTGAATGCCCTGAAAGTGGTGAAAAAGAGCCTAGACGAGGTGCGCATTGT
CCTCAACGGGGCCGGGGCAGCCGGCATTGCCGTGACGCAACTGCTGCGGCGGGCCGGGGCAGCCCACATCCTGGTCTGCG
ACCGCCAAGGGATCCTTAGCCGCTCCCGTTCCGATCTCACCCCCGAGAAGGCGGCCATTGCCGTCGAGGAGAAGGGATCC
CTGGCGGAAGCCTTGGTGGGAGCGGATGTGTTTATTGGGGTGAGCGTGGCCAATGCCCTGACAGTGGAGATGGTAGAAAA
AATGGCACCGGATCCCATCGTCTTTGCCCTGGCCAACCCGGTGCCCGAGATCCAGCCGGAGCTGGTGGGCAATCGGGTGG
CAGTGATGGCCACGGGCCGCAGCGACTACCCCAACCAAATCAACAACGTGCTGGCCTTTCCGGGCGTGTTTCGCGGCGCC
CTCGACTGCCGCGCCAAGCAGATCAACACCGAGATGTGTGTGGCTGCCGCTCAGGCCATTGCTGCCTTGATCCCTGCCGA
AGAGTTGGATCCCCAGTGGATCATCCCCTCGGTCTTCGACGGGCGGGTGGTTCCTCAGGTGGCCGCTGCGGTAATCCAGG
CTGCCCGCCAAACCGGCGTGGCCGGGATCCCTTGA

Upstream 100 bases:

>100_bases
GGCTCTGCCATCGCCTGGGTGCGGTTGCTGTAGTAGCCCTCCAGCCAGTTGGCCAGGGTGTGCAAGTCGGAAGCGGATTT
TTCGTCTACCATAGGGAGCT

Downstream 100 bases:

>100_bases
GGGCTACCTAGGGCACCAGGTGACAAGCCACAAAATGCCCCGCGCTCACCTCGCGAAAGGCCGGGGGTGGATCTTGGCCT
GTTCCTTCCCTGGGGGGAAC

Product: malate dehydrogenase (oxaloacetate-decarboxylating)

Products: NA

Alternate protein names: NAD-ME [H]

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MTKLTPSSSYSIALRIRLLNQPGALARVTQAIAEVGGSLSQIDLIHQSAAETVREICVDAVSSEHAQQIYTAVASLANVK
VERVVDRTFELHRGGKLQVVAKSPLESAADLAMAYTPGVGRVSLEIAANPAAVYEYTIKGNSVGIVTDGSAVLGLGNVGP
EAALPVMEGKAALFKRFADIDAFPLCLATQEVGEIVETVVRLAPGFGGFNLEDISAPRCFAIESQLKARLNLPVFHDDQH
GTAIVVLAALLNALKVVKKSLDEVRIVLNGAGAAGIAVTQLLRRAGAAHILVCDRQGILSRSRSDLTPEKAAIAVEEKGS
LAEALVGADVFIGVSVANALTVEMVEKMAPDPIVFALANPVPEIQPELVGNRVAVMATGRSDYPNQINNVLAFPGVFRGA
LDCRAKQINTEMCVAAAQAIAALIPAEELDPQWIIPSVFDGRVVPQVAAAVIQAARQTGVAGIP

Sequences:

>Translated_464_residues
MTKLTPSSSYSIALRIRLLNQPGALARVTQAIAEVGGSLSQIDLIHQSAAETVREICVDAVSSEHAQQIYTAVASLANVK
VERVVDRTFELHRGGKLQVVAKSPLESAADLAMAYTPGVGRVSLEIAANPAAVYEYTIKGNSVGIVTDGSAVLGLGNVGP
EAALPVMEGKAALFKRFADIDAFPLCLATQEVGEIVETVVRLAPGFGGFNLEDISAPRCFAIESQLKARLNLPVFHDDQH
GTAIVVLAALLNALKVVKKSLDEVRIVLNGAGAAGIAVTQLLRRAGAAHILVCDRQGILSRSRSDLTPEKAAIAVEEKGS
LAEALVGADVFIGVSVANALTVEMVEKMAPDPIVFALANPVPEIQPELVGNRVAVMATGRSDYPNQINNVLAFPGVFRGA
LDCRAKQINTEMCVAAAQAIAALIPAEELDPQWIIPSVFDGRVVPQVAAAVIQAARQTGVAGIP
>Mature_463_residues
TKLTPSSSYSIALRIRLLNQPGALARVTQAIAEVGGSLSQIDLIHQSAAETVREICVDAVSSEHAQQIYTAVASLANVKV
ERVVDRTFELHRGGKLQVVAKSPLESAADLAMAYTPGVGRVSLEIAANPAAVYEYTIKGNSVGIVTDGSAVLGLGNVGPE
AALPVMEGKAALFKRFADIDAFPLCLATQEVGEIVETVVRLAPGFGGFNLEDISAPRCFAIESQLKARLNLPVFHDDQHG
TAIVVLAALLNALKVVKKSLDEVRIVLNGAGAAGIAVTQLLRRAGAAHILVCDRQGILSRSRSDLTPEKAAIAVEEKGSL
AEALVGADVFIGVSVANALTVEMVEKMAPDPIVFALANPVPEIQPELVGNRVAVMATGRSDYPNQINNVLAFPGVFRGAL
DCRAKQINTEMCVAAAQAIAALIPAEELDPQWIIPSVFDGRVVPQVAAAVIQAARQTGVAGIP

Specific function: In addition to the NAD-dependent oxidative decarboxylation of L-malate, the enzyme catalyzes the decarboxylation of oxaloacetate [H]

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=385, Percent_Identity=25.974025974026, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI62420882, Length=385, Percent_Identity=26.2337662337662, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI62420880, Length=385, Percent_Identity=26.2337662337662, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI239049447, Length=385, Percent_Identity=26.2337662337662, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI4505145, Length=359, Percent_Identity=25.9052924791086, Blast_Score=73, Evalue=7e-13,
Organism=Escherichia coli, GI1788806, Length=391, Percent_Identity=48.849104859335, Blast_Score=353, Evalue=1e-98,
Organism=Escherichia coli, GI87081919, Length=281, Percent_Identity=27.7580071174377, Blast_Score=79, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17537199, Length=405, Percent_Identity=26.9135802469136, Blast_Score=86, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6322823, Length=280, Percent_Identity=31.7857142857143, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24646388, Length=390, Percent_Identity=27.6923076923077, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24646386, Length=390, Percent_Identity=27.6923076923077, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21356279, Length=442, Percent_Identity=25.7918552036199, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI281362672, Length=442, Percent_Identity=25.7918552036199, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI281362674, Length=442, Percent_Identity=25.7918552036199, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI78707242, Length=358, Percent_Identity=25.6983240223464, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI78707238, Length=358, Percent_Identity=25.6983240223464, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI78707240, Length=358, Percent_Identity=25.6983240223464, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI78707232, Length=435, Percent_Identity=23.9080459770115, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI281363503, Length=435, Percent_Identity=23.9080459770115, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI281363505, Length=435, Percent_Identity=23.9080459770115, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI78707236, Length=435, Percent_Identity=23.9080459770115, Blast_Score=79, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 48615; Mature: 48484

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLTPSSSYSIALRIRLLNQPGALARVTQAIAEVGGSLSQIDLIHQSAAETVREICVDA
CCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
VSSEHAQQIYTAVASLANVKVERVVDRTFELHRGGKLQVVAKSPLESAADLAMAYTPGVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHEEECCCCC
RVSLEIAANPAAVYEYTIKGNSVGIVTDGSAVLGLGNVGPEAALPVMEGKAALFKRFADI
EEEEEEECCCCEEEEEEEECCEEEEEECCCEEEEECCCCCHHCCCHHCCHHHHHHHHHCC
DAFPLCLATQEVGEIVETVVRLAPGFGGFNLEDISAPRCFAIESQLKARLNLPVFHDDQH
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEHHHHHHHCCCCEEECCCC
GTAIVVLAALLNALKVVKKSLDEVRIVLNGAGAAGIAVTQLLRRAGAAHILVCDRQGILS
CHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCHHHH
RSRSDLTPEKAAIAVEEKGSLAEALVGADVFIGVSVANALTVEMVEKMAPDPIVFALANP
CCCCCCCCCHHEEEEECCCCHHHHHHCCHHEEEHHHHHHHHHHHHHHHCCCCEEEEECCC
VPEIQPELVGNRVAVMATGRSDYPNQINNVLAFPGVFRGALDCRAKQINTEMCVAAAQAI
CCCCCHHHCCCEEEEEEECCCCCCHHHHHHEECCHHHHHHHHHHHHHCCHHHHHHHHHHH
AALIPAEELDPQWIIPSVFDGRVVPQVAAAVIQAARQTGVAGIP
HHHCCHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TKLTPSSSYSIALRIRLLNQPGALARVTQAIAEVGGSLSQIDLIHQSAAETVREICVDA
CCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
VSSEHAQQIYTAVASLANVKVERVVDRTFELHRGGKLQVVAKSPLESAADLAMAYTPGVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHEEECCCCC
RVSLEIAANPAAVYEYTIKGNSVGIVTDGSAVLGLGNVGPEAALPVMEGKAALFKRFADI
EEEEEEECCCCEEEEEEEECCEEEEEECCCEEEEECCCCCHHCCCHHCCHHHHHHHHHCC
DAFPLCLATQEVGEIVETVVRLAPGFGGFNLEDISAPRCFAIESQLKARLNLPVFHDDQH
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEHHHHHHHCCCCEEECCCC
GTAIVVLAALLNALKVVKKSLDEVRIVLNGAGAAGIAVTQLLRRAGAAHILVCDRQGILS
CHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCHHHH
RSRSDLTPEKAAIAVEEKGSLAEALVGADVFIGVSVANALTVEMVEKMAPDPIVFALANP
CCCCCCCCCHHEEEEECCCCHHHHHHCCHHEEEHHHHHHHHHHHHHHHCCCCEEEEECCC
VPEIQPELVGNRVAVMATGRSDYPNQINNVLAFPGVFRGALDCRAKQINTEMCVAAAQAI
CCCCCHHHCCCEEEEEEECCCCCCHHHHHHEECCHHHHHHHHHHHHHCCHHHHHHHHHHH
AALIPAEELDPQWIIPSVFDGRVVPQVAAAVIQAARQTGVAGIP
HHHCCHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2644282 [H]