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The map label for this gene is clpP-1 [H]

Identifier: 86608098

GI number: 86608098

Start: 620234

End: 620869

Strand: Direct

Name: clpP-1 [H]

Synonym: CYB_0608

Alternate gene names: 86608098

Gene position: 620234-620869 (Clockwise)

Preceding gene: 86608097

Following gene: 86608099

Centisome position: 20.36

GC content: 56.13

Gene sequence:

>636_bases
ATGGTGCAATCGACCTACTACTACTATGGCGGCGCTCCGGTTCGTACCCCACCCCCAGATTTGGCTTCGCTGCTGCTGCA
GGAGCGCATTGTCTACCTGGGCTTGCCCCTTGCAGAGGAGTCGAATGCCAACGTCACCAAGCTGATCATCGAGCAACTGC
TGTATCTGCAGTACGAGGATCGGGAAAAGCCGATCAAGATGTACATCAACTCGACGGGCACCTATAACTACGACACAGCG
GCCTTTGCCATTCTGGACACCATGAACTACATCAAGCCGCCTGTGCATACTATCTGCATCGGCAATGCCATTGGGATGGC
GGCCATGTTATTGGCAGCAGGAGCCAAAGGATTTCGGGCCAGTCTGCCCAATGCCACGATCGTGCTCCACCAGCCCTATG
GGGGCACCCGTGGACAGGCCAGCGACATCGAAATTCGCACCCGCGAGATTCTGACTTTGCGCAACATGATGCTGGAGCAA
CTGTCCCGCAGCACGGGCCAGCCTATGGAGCGCATCAAGAAGGACACGGAGCGGCTATTTTTCATGACACCAGAGGAAGC
CAAAGCCTACGGCCTCATCGATCAGGTGCTGACCAGTTCCAAAGGCGTGCCGAAGTTGGCAGCAGCCAAAAACTAA

Upstream 100 bases:

>100_bases
CAAGGGATCCGTTGGGATGCTGCGGATCCCTTGTGGTCCTCAAGTGTTGTTTTTCTGGTGTTTTTCAGTTGAACAGACCC
AGGCGTAGGAGATCTCTGGC

Downstream 100 bases:

>100_bases
AGCAGAGGGATCCGTCCATGGTTCAAGTTCCCGTACAGCCTTTAACGGCGGAGGAGTACCTCCACCTCACGGACTGGCCT
TATGGAGGACGGATTGAACT

Product: ATP-dependent Clp protease-like protein

Products: NA

Alternate protein names: Endopeptidase Clp-like [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MVQSTYYYYGGAPVRTPPPDLASLLLQERIVYLGLPLAEESNANVTKLIIEQLLYLQYEDREKPIKMYINSTGTYNYDTA
AFAILDTMNYIKPPVHTICIGNAIGMAAMLLAAGAKGFRASLPNATIVLHQPYGGTRGQASDIEIRTREILTLRNMMLEQ
LSRSTGQPMERIKKDTERLFFMTPEEAKAYGLIDQVLTSSKGVPKLAAAKN

Sequences:

>Translated_211_residues
MVQSTYYYYGGAPVRTPPPDLASLLLQERIVYLGLPLAEESNANVTKLIIEQLLYLQYEDREKPIKMYINSTGTYNYDTA
AFAILDTMNYIKPPVHTICIGNAIGMAAMLLAAGAKGFRASLPNATIVLHQPYGGTRGQASDIEIRTREILTLRNMMLEQ
LSRSTGQPMERIKKDTERLFFMTPEEAKAYGLIDQVLTSSKGVPKLAAAKN
>Mature_211_residues
MVQSTYYYYGGAPVRTPPPDLASLLLQERIVYLGLPLAEESNANVTKLIIEQLLYLQYEDREKPIKMYINSTGTYNYDTA
AFAILDTMNYIKPPVHTICIGNAIGMAAMLLAAGAKGFRASLPNATIVLHQPYGGTRGQASDIEIRTREILTLRNMMLEQ
LSRSTGQPMERIKKDTERLFFMTPEEAKAYGLIDQVLTSSKGVPKLAAAKN

Specific function: Has lost the two conserved residues (Ser and His) proposed to be part of the active site. Therefore it could be inactive [H]

COG id: COG0740

COG function: function code OU; Protease subunit of ATP-dependent Clp proteases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S14 family [H]

Homologues:

Organism=Homo sapiens, GI5174419, Length=179, Percent_Identity=45.8100558659218, Blast_Score=155, Evalue=2e-38,
Organism=Escherichia coli, GI1786641, Length=179, Percent_Identity=49.1620111731844, Blast_Score=164, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17538017, Length=185, Percent_Identity=43.2432432432432, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI20129427, Length=178, Percent_Identity=45.5056179775281, Blast_Score=155, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001907 [H]

Pfam domain/function: PF00574 CLP_protease [H]

EC number: 3.4.21.92

Molecular weight: Translated: 23441; Mature: 23441

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQSTYYYYGGAPVRTPPPDLASLLLQERIVYLGLPLAEESNANVTKLIIEQLLYLQYED
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCC
REKPIKMYINSTGTYNYDTAAFAILDTMNYIKPPVHTICIGNAIGMAAMLLAAGAKGFRA
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCHHEEEECHHHHHHHHHHHHCCCCCCC
SLPNATIVLHQPYGGTRGQASDIEIRTREILTLRNMMLEQLSRSTGQPMERIKKDTERLF
CCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEE
FMTPEEAKAYGLIDQVLTSSKGVPKLAAAKN
EECCCHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MVQSTYYYYGGAPVRTPPPDLASLLLQERIVYLGLPLAEESNANVTKLIIEQLLYLQYED
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCC
REKPIKMYINSTGTYNYDTAAFAILDTMNYIKPPVHTICIGNAIGMAAMLLAAGAKGFRA
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCHHEEEECHHHHHHHHHHHHCCCCCCC
SLPNATIVLHQPYGGTRGQASDIEIRTREILTLRNMMLEQLSRSTGQPMERIKKDTERLF
CCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEE
FMTPEEAKAYGLIDQVLTSSKGVPKLAAAKN
EECCCHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP imidodiphosphate; dATP degradation, [C]

Metal ions: Ca2+; Mg2+; Mn2+; Zn2+ [C]

Kcat value (1/min): 800 [C]

Specific activity: NA

Km value (mM): 0.21 {ATP}} 1.3 {N-succinyl-Leu-Tyr} [C]

Substrates: NA

Specific reaction: NA

General reaction: Hydrolysis of peptide bonds [C]

Inhibitor: Diisopropyl fluorophosphate ClpAP; Fluorosulfonyl benzoyl adenosine; Kappa-Casein; Mg2+; NEM hydrolysis; Neohydrin; Succinyl -Leu-Tyr4-methyl coumarin7-amide; Xaa-Tyr-Leu-Tyr-Trp [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]