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The map label for this gene is pgl [H]

Identifier: 86361024

GI number: 86361024

Start: 327316

End: 328680

Strand: Reverse

Name: pgl [H]

Synonym: RHE_PF00294

Alternate gene names: 86361024

Gene position: 328680-327316 (Counterclockwise)

Preceding gene: 86361025

Following gene: 86361023

Centisome position: 51.16

GC content: 64.4

Gene sequence:

>1365_bases
ATGAGTACCACCTCCCGCGTCGCGATCGAGGCGTTCGACGGCGATAATACCGATGGCCTGCAGGCTGCGATCGACACTCT
TTCGGCTTCCGGCGGCGGACGCCTGGAGCTCCTGGCGGGCATCCACCGCTGCCAGGGGCTCCGGCTGCGCTCCGGGGTCG
ATCTGCACCTTGCCGCCGGCGCGATCTTGCGGCCCGTCCCCGACTATGCGGCCTACGCGCATACAACGGTTTCGGTGATC
GCCGAAAAATCCGATCGAGGCATGATCGTCGCGCGGGATGCGCGGCGCATCAGCCTGACGGGGGAGGGTCGCATCGAAGC
CGGCTGCGAAAGCTTCATCACAGGAGACGACGAGACGGTGGGAACTTTCATTCCGGCCGAATTCCGTCCCCGTGTCGTCG
TCCTCGAAGGCTGCGACGAGGTCGAGATCAGTTCCGTCACCATCAGCCGCTCGCCGATGTGGACGCTGCATTTCGTCAAC
TGCACCGATGTCGCGGTCCGCAGCGTCACCATCGAAAACGATCGCCGCCTTCCCAATACGGACGGCATCGTGCTCGACGC
CTGCCGCGGCGCCGTCATTGAGGACTGTAACATATCGACGGCCGACGACGGCATATGCCTGAAGACGAGCATCGGTCCTG
AAGGTGTGGCCATCGGCCGGTGCGAGAACATTCTCGTGCGCCGCTGTTTCGTTCAGAGCTTGAGCTGTGCGTTGAAGATC
GGCACGGAAACGCATGGCGATGTCACCAATGTCCTGTTTGAGGATTGCAGCGTCTCGTCTTCCAACCGGGCGCTCGGCAT
ATTTTCCCGCGACGGCGGCCGGATCTCGAATGTGAGGTTTTCCAGGATTTCGGTAGAGTGCCGGGAAACGCCGGATGGCT
TCTGGGGCTCCGGGGAGGCGCTGACCGTCAATGTCGTCGACCGGATCGCCGAGCGGCCTGCGGGCGCCGTCGAAAATCTC
GTCGTCGAAGACGTGACCGGCCGCATGGAAGGGGCGATCACCATAATCGCGGCTGCGCCGGCGGGCATCAGCAACGTATC
CCTCGCCCGGATCGCCATCGATCAGCAGCCCGGGCAGCTCGGCACCGGGCGGACCTACGATCTGCGCCCGACGAATGCCG
ACCTCGCGCCGAAGGCCGATGGCGGCGGGCGTGCCAATGCCTGGACTCGGGGTTCGGACGGGCGGGTGATCGGCCTGCAG
GACTATCCCGGCGGAATGCCGGCGGTCTACGTGGCCGATGTCGCCGGGATATCGATGAACAAGGTGCGGATCACGAGGCC
TGGACCGCTGCCGCAAGGCTGGAACGCAATCGACGTCGTCATGGAAACGACGGCATCTGATGGGAGTGGGGCATGGCAGA
ACTGA

Upstream 100 bases:

>100_bases
TCGATTTCTACCGGCGCATCCCGAACCTGCCGACGCCCTACGGCCAGGCGCTGACCATGCTGCTGCTGACCGAAGTCTTC
ATCGAGAGCGGCCGACGCCA

Downstream 100 bases:

>100_bases
AACTTTCCACCGTCAACAAGTCGTATGGCGCCGTCAAAATCCTGCATGACGTCGAACTCGACATCAAGGACGGCGAGTTC
GTCGTCTTTGTCGGACCGTC

Product: polygalacturonase protein

Products: NA

Alternate protein names: PGL; Pectinase [H]

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MSTTSRVAIEAFDGDNTDGLQAAIDTLSASGGGRLELLAGIHRCQGLRLRSGVDLHLAAGAILRPVPDYAAYAHTTVSVI
AEKSDRGMIVARDARRISLTGEGRIEAGCESFITGDDETVGTFIPAEFRPRVVVLEGCDEVEISSVTISRSPMWTLHFVN
CTDVAVRSVTIENDRRLPNTDGIVLDACRGAVIEDCNISTADDGICLKTSIGPEGVAIGRCENILVRRCFVQSLSCALKI
GTETHGDVTNVLFEDCSVSSSNRALGIFSRDGGRISNVRFSRISVECRETPDGFWGSGEALTVNVVDRIAERPAGAVENL
VVEDVTGRMEGAITIIAAAPAGISNVSLARIAIDQQPGQLGTGRTYDLRPTNADLAPKADGGGRANAWTRGSDGRVIGLQ
DYPGGMPAVYVADVAGISMNKVRITRPGPLPQGWNAIDVVMETTASDGSGAWQN

Sequences:

>Translated_454_residues
MSTTSRVAIEAFDGDNTDGLQAAIDTLSASGGGRLELLAGIHRCQGLRLRSGVDLHLAAGAILRPVPDYAAYAHTTVSVI
AEKSDRGMIVARDARRISLTGEGRIEAGCESFITGDDETVGTFIPAEFRPRVVVLEGCDEVEISSVTISRSPMWTLHFVN
CTDVAVRSVTIENDRRLPNTDGIVLDACRGAVIEDCNISTADDGICLKTSIGPEGVAIGRCENILVRRCFVQSLSCALKI
GTETHGDVTNVLFEDCSVSSSNRALGIFSRDGGRISNVRFSRISVECRETPDGFWGSGEALTVNVVDRIAERPAGAVENL
VVEDVTGRMEGAITIIAAAPAGISNVSLARIAIDQQPGQLGTGRTYDLRPTNADLAPKADGGGRANAWTRGSDGRVIGLQ
DYPGGMPAVYVADVAGISMNKVRITRPGPLPQGWNAIDVVMETTASDGSGAWQN
>Mature_453_residues
STTSRVAIEAFDGDNTDGLQAAIDTLSASGGGRLELLAGIHRCQGLRLRSGVDLHLAAGAILRPVPDYAAYAHTTVSVIA
EKSDRGMIVARDARRISLTGEGRIEAGCESFITGDDETVGTFIPAEFRPRVVVLEGCDEVEISSVTISRSPMWTLHFVNC
TDVAVRSVTIENDRRLPNTDGIVLDACRGAVIEDCNISTADDGICLKTSIGPEGVAIGRCENILVRRCFVQSLSCALKIG
TETHGDVTNVLFEDCSVSSSNRALGIFSRDGGRISNVRFSRISVECRETPDGFWGSGEALTVNVVDRIAERPAGAVENLV
VEDVTGRMEGAITIIAAAPAGISNVSLARIAIDQQPGQLGTGRTYDLRPTNADLAPKADGGGRANAWTRGSDGRVIGLQD
YPGGMPAVYVADVAGISMNKVRITRPGPLPQGWNAIDVVMETTASDGSGAWQN

Specific function: Seems to regulates the surface properties of the bacterium in the presence of plant cells or plant cell extracts [H]

COG id: COG5434

COG function: function code M; Endopolygalacturonase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PbH1 repeats [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000743
- InterPro:   IPR006626
- InterPro:   IPR012334
- InterPro:   IPR011050 [H]

Pfam domain/function: PF00295 Glyco_hydro_28 [H]

EC number: =3.2.1.15 [H]

Molecular weight: Translated: 47959; Mature: 47827

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTSRVAIEAFDGDNTDGLQAAIDTLSASGGGRLELLAGIHRCQGLRLRSGVDLHLAAG
CCCCCEEEEEEECCCCCCHHHHHHHHHCCCCCCEEHHHHHHHHHCCEEECCCCEEEEECC
AILRPVPDYAAYAHTTVSVIAEKSDRGMIVARDARRISLTGEGRIEAGCESFITGDDETV
HHHCCCCCHHHHHHEEEEEEEECCCCCEEEEECCEEEEEECCCCCCCCHHHHCCCCCCCE
GTFIPAEFRPRVVVLEGCDEVEISSVTISRSPMWTLHFVNCTDVAVRSVTIENDRRLPNT
EEECCCCCCCCEEEEECCCCEEEEEEEEECCCEEEEEEECCCCEEEEEEEECCCCCCCCC
DGIVLDACRGAVIEDCNISTADDGICLKTSIGPEGVAIGRCENILVRRCFVQSLSCALKI
CCEEEECCCCCEEECCCCCCCCCCEEEEECCCCCCEEECHHHHHHHHHHHHHHCEEEEEE
GTETHGDVTNVLFEDCSVSSSNRALGIFSRDGGRISNVRFSRISVECRETPDGFWGSGEA
CCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEEEEEEEEEEECCCCCCCCCCCE
LTVNVVDRIAERPAGAVENLVVEDVTGRMEGAITIIAAAPAGISNVSLARIAIDQQPGQL
EEEHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEEEEEECCCCCC
GTGRTYDLRPTNADLAPKADGGGRANAWTRGSDGRVIGLQDYPGGMPAVYVADVAGISMN
CCCCEEECCCCCCCCCCCCCCCCCCCCEECCCCCCEEEECCCCCCCCEEEEEEECCCEEC
KVRITRPGPLPQGWNAIDVVMETTASDGSGAWQN
EEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
STTSRVAIEAFDGDNTDGLQAAIDTLSASGGGRLELLAGIHRCQGLRLRSGVDLHLAAG
CCCCEEEEEEECCCCCCHHHHHHHHHCCCCCCEEHHHHHHHHHCCEEECCCCEEEEECC
AILRPVPDYAAYAHTTVSVIAEKSDRGMIVARDARRISLTGEGRIEAGCESFITGDDETV
HHHCCCCCHHHHHHEEEEEEEECCCCCEEEEECCEEEEEECCCCCCCCHHHHCCCCCCCE
GTFIPAEFRPRVVVLEGCDEVEISSVTISRSPMWTLHFVNCTDVAVRSVTIENDRRLPNT
EEECCCCCCCCEEEEECCCCEEEEEEEEECCCEEEEEEECCCCEEEEEEEECCCCCCCCC
DGIVLDACRGAVIEDCNISTADDGICLKTSIGPEGVAIGRCENILVRRCFVQSLSCALKI
CCEEEECCCCCEEECCCCCCCCCCEEEEECCCCCCEEECHHHHHHHHHHHHHHCEEEEEE
GTETHGDVTNVLFEDCSVSSSNRALGIFSRDGGRISNVRFSRISVECRETPDGFWGSGEA
CCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEEEEEEEEEEECCCCCCCCCCCE
LTVNVVDRIAERPAGAVENLVVEDVTGRMEGAITIIAAAPAGISNVSLARIAIDQQPGQL
EEEHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEEEEEECCCCCC
GTGRTYDLRPTNADLAPKADGGGRANAWTRGSDGRVIGLQDYPGGMPAVYVADVAGISMN
CCCCEEECCCCCCCCCCCCCCCCCCCCEECCCCCCEEEECCCCCCCCEEEEEEECCCEEC
KVRITRPGPLPQGWNAIDVVMETTASDGSGAWQN
EEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1860822 [H]