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The map label for this gene is pucI [H]

Identifier: 86361012

GI number: 86361012

Start: 311590

End: 312876

Strand: Reverse

Name: pucI [H]

Synonym: RHE_PF00282

Alternate gene names: 86361012

Gene position: 312876-311590 (Counterclockwise)

Preceding gene: 86361013

Following gene: 86361011

Centisome position: 48.7

GC content: 61.54

Gene sequence:

>1287_bases
GTGAACTTCGTCATCGCCATCGGTATCGGCTCTCTCGTCATCTTCTTCCTGATGAGCCTGGTCGGAAATGCCGGCGTGCG
CACGGGCGTGCCCTTTCCGGTTCTGGCGCGCGCCTCCTTCGGCACCTTCGGCGCCAATGTTCCAGCGCTCGTCCGGGCGG
TCGTCGCCTGCTTCTGGTACGGGGCGCAGACCGCCGCCGCATCCGGTGCGATCGTCGCCCTCCTGATCAGGAATGAGAGC
CTGCTCGCCTTCCATCAGAACAGCCACCTGCTCGGCCACTCGACCCTCGAACTCATCTGCTACGTCATCGTCTGGGCACT
GCAGCTGCTGATCATTCAGCGCGGCATGGAAACGGTCCGCAAGTTCCAGGATTGGGCCGGCCCTGCCGTCTGGATCATGA
TGCTGATCCTCGCGGTTTATCTGGTCGCCAAATCAGGCACCTTTTCCTTCGGTTCGGAGATCCCGCGCGACGTGCTGATC
GAAAAGACCAAGGATGCCGGCGTGCCGGGTGAACCCGGCTCGATTGCGGCGCTTGCCGCTGTCGCCGCCACCTGGCTTAC
CTATTTCGCTGCGCTCTATCTGAATTTCTGCGATTTCTCGCGTTACGCAACGAGCGAAAAGGCGCTGCGGAAGGGCAATC
TCTGGGGCCTGCCGATCAATCTTCTGGCCTTCTGCCTCGTCGCAGGCGTCACGACCACGGCCGCCTTCACCGTCTACGGC
GAGGTGCTGCTGCATCCGGAAATGATCTCTGCGAAATTTGACAGCTGGTTCCTAGCGCTGCTTGCAGCGCTGACATTCGC
GGTCGCCACGCTCGGCATCAACGTCGTGGCAAACTTCGTCTCGCCGGCCTTCGACTTCGCCAATGTCTTCCCGCGCCAGA
TCAACTTCAAGCGCGGCGGATATATCGCCGCGCTGATCGCCCTCGTGCTCTATCCATTCGCTCCCTGGGAGACGGGTGCG
GCGCACTTCGTCAACTTCATCGGTTCGACGATGGGGCCGATCTTCGGCATCATGATGGTCGACTACTATCTCATTCGGAA
GAGCCAGCTGAACGTAGAGGCGCTCTATCAGGAGAATGGCGAGTTCCGGTTCCAGAACGGCTGGCACGGCAATGCCTTCA
TCGCCTTTGCGGTCGGTGCGCTGTTCTCCTCGATCCTGCCGACCTTCACCTCCATCCTGCCCGACTGGTGGGGCACCTAT
GGCTGGTTCTTCGGCGTCGCCATCGGCGGGGCGATCTATTTCGTGCTGAGAATGGGCGCGCGGCGCACTCCGGCCTTCGC
ATCATGA

Upstream 100 bases:

>100_bases
GTGGGGTGCATTCAGCATCTTCAACGTCTGGACATCGGACGTCCATAGTCTCTGGGGCTATTATCTGGCGGCGAGCCTGT
TCCTGCTCTGCGGCAGCTTC

Downstream 100 bases:

>100_bases
CGAAACAAAGGCGCCTGGCCATCGCTAGGCGCCTTTATGTTCCGTTTGGAAAAGGAGCCGGTCATTGGCCACGTTCAACG
GCGCGCCGCCCTCTGGCGAT

Product: nucleotide permease protein

Products: NA

Alternate protein names: Allantoin transport protein [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MNFVIAIGIGSLVIFFLMSLVGNAGVRTGVPFPVLARASFGTFGANVPALVRAVVACFWYGAQTAAASGAIVALLIRNES
LLAFHQNSHLLGHSTLELICYVIVWALQLLIIQRGMETVRKFQDWAGPAVWIMMLILAVYLVAKSGTFSFGSEIPRDVLI
EKTKDAGVPGEPGSIAALAAVAATWLTYFAALYLNFCDFSRYATSEKALRKGNLWGLPINLLAFCLVAGVTTTAAFTVYG
EVLLHPEMISAKFDSWFLALLAALTFAVATLGINVVANFVSPAFDFANVFPRQINFKRGGYIAALIALVLYPFAPWETGA
AHFVNFIGSTMGPIFGIMMVDYYLIRKSQLNVEALYQENGEFRFQNGWHGNAFIAFAVGALFSSILPTFTSILPDWWGTY
GWFFGVAIGGAIYFVLRMGARRTPAFAS

Sequences:

>Translated_428_residues
MNFVIAIGIGSLVIFFLMSLVGNAGVRTGVPFPVLARASFGTFGANVPALVRAVVACFWYGAQTAAASGAIVALLIRNES
LLAFHQNSHLLGHSTLELICYVIVWALQLLIIQRGMETVRKFQDWAGPAVWIMMLILAVYLVAKSGTFSFGSEIPRDVLI
EKTKDAGVPGEPGSIAALAAVAATWLTYFAALYLNFCDFSRYATSEKALRKGNLWGLPINLLAFCLVAGVTTTAAFTVYG
EVLLHPEMISAKFDSWFLALLAALTFAVATLGINVVANFVSPAFDFANVFPRQINFKRGGYIAALIALVLYPFAPWETGA
AHFVNFIGSTMGPIFGIMMVDYYLIRKSQLNVEALYQENGEFRFQNGWHGNAFIAFAVGALFSSILPTFTSILPDWWGTY
GWFFGVAIGGAIYFVLRMGARRTPAFAS
>Mature_428_residues
MNFVIAIGIGSLVIFFLMSLVGNAGVRTGVPFPVLARASFGTFGANVPALVRAVVACFWYGAQTAAASGAIVALLIRNES
LLAFHQNSHLLGHSTLELICYVIVWALQLLIIQRGMETVRKFQDWAGPAVWIMMLILAVYLVAKSGTFSFGSEIPRDVLI
EKTKDAGVPGEPGSIAALAAVAATWLTYFAALYLNFCDFSRYATSEKALRKGNLWGLPINLLAFCLVAGVTTTAAFTVYG
EVLLHPEMISAKFDSWFLALLAALTFAVATLGINVVANFVSPAFDFANVFPRQINFKRGGYIAALIALVLYPFAPWETGA
AHFVNFIGSTMGPIFGIMMVDYYLIRKSQLNVEALYQENGEFRFQNGWHGNAFIAFAVGALFSSILPTFTSILPDWWGTY
GWFFGVAIGGAIYFVLRMGARRTPAFAS

Specific function: Transport of allantoin [H]

COG id: COG1953

COG function: function code FH; Cytosine/uracil/thiamine/allantoin permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI87081749, Length=436, Percent_Identity=27.0642201834862, Blast_Score=130, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6322219, Length=374, Percent_Identity=25.1336898395722, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6319429, Length=415, Percent_Identity=24.0963855421687, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6319495, Length=386, Percent_Identity=24.3523316062176, Blast_Score=90, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6323266, Length=374, Percent_Identity=22.9946524064171, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6324766, Length=391, Percent_Identity=21.4833759590793, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6324645, Length=383, Percent_Identity=21.4099216710183, Blast_Score=67, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 46528; Mature: 46528

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFVIAIGIGSLVIFFLMSLVGNAGVRTGVPFPVLARASFGTFGANVPALVRAVVACFWY
CCEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHH
GAQTAAASGAIVALLIRNESLLAFHQNSHLLGHSTLELICYVIVWALQLLIIQRGMETVR
CHHHHHCCCCEEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFQDWAGPAVWIMMLILAVYLVAKSGTFSFGSEIPRDVLIEKTKDAGVPGEPGSIAALAA
HHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHH
VAATWLTYFAALYLNFCDFSRYATSEKALRKGNLWGLPINLLAFCLVAGVTTTAAFTVYG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
EVLLHPEMISAKFDSWFLALLAALTFAVATLGINVVANFVSPAFDFANVFPRQINFKRGG
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCC
YIAALIALVLYPFAPWETGAAHFVNFIGSTMGPIFGIMMVDYYLIRKSQLNVEALYQENG
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCC
EFRFQNGWHGNAFIAFAVGALFSSILPTFTSILPDWWGTYGWFFGVAIGGAIYFVLRMGA
CEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RRTPAFAS
CCCCCCCC
>Mature Secondary Structure
MNFVIAIGIGSLVIFFLMSLVGNAGVRTGVPFPVLARASFGTFGANVPALVRAVVACFWY
CCEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHH
GAQTAAASGAIVALLIRNESLLAFHQNSHLLGHSTLELICYVIVWALQLLIIQRGMETVR
CHHHHHCCCCEEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFQDWAGPAVWIMMLILAVYLVAKSGTFSFGSEIPRDVLIEKTKDAGVPGEPGSIAALAA
HHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHH
VAATWLTYFAALYLNFCDFSRYATSEKALRKGNLWGLPINLLAFCLVAGVTTTAAFTVYG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
EVLLHPEMISAKFDSWFLALLAALTFAVATLGINVVANFVSPAFDFANVFPRQINFKRGG
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCC
YIAALIALVLYPFAPWETGAAHFVNFIGSTMGPIFGIMMVDYYLIRKSQLNVEALYQENG
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCC
EFRFQNGWHGNAFIAFAVGALFSSILPTFTSILPDWWGTYGWFFGVAIGGAIYFVLRMGA
CEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RRTPAFAS
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 11344136 [H]