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The map label for this gene is glgBf

Identifier: 86361005

GI number: 86361005

Start: 299235

End: 301433

Strand: Reverse

Name: glgBf

Synonym: RHE_PF00275

Alternate gene names: 86361005

Gene position: 301433-299235 (Counterclockwise)

Preceding gene: 86361006

Following gene: 86361002

Centisome position: 46.91

GC content: 62.76

Gene sequence:

>2199_bases
ATGAATGTTGAACGCTCTGAATTTCTCGCAGGCGTCGGGCACGATGCGCTCTGGGCCTTGATCGAGGGGCGCCACGGCGA
TCCATTCTCGATCCTCGGTCCGCACGAGAGCGGCGGTATGACGATCGTGCGCGTCTATCTGCCGGGCGCCGAAGGCGTCG
ATCTCATTGAAGCAGCCAGTGGCAGGGTGGTGACGCCCTTCAGCATCGCTCACCCCTCCGGCCTGTTTGCGGCGGCGATG
GGTTCGAGGATGCACTACCGGTTGCGGATCACATGGCCGGATGGCGAGCAGATCACCGAAGACCCCTATAGCTTCGGGCT
TCTGCTTGGAGAGCTCGATCTCCACCTGATCTCCGAGGGCACCCATTACAGCCTGAGCCGGACGCTCGGCGCGGTCGAAA
TGGCGATCGACGACGTCGCGGGCGTTCGCTTCGCCGTCTGGGCGCCGAATGCCCGCCGCGTTTCGGTTGTCGGCGATTTC
AATGCCTGGGACGGGCGGCGAAACCCGATGCGGCTGCGGCAGTCCGCCGGCGTCTGGGAGCTGTTCATGCCCCGGCTGGC
GCCCGGCGAAAGATACAAGTTCGAGATCATCGATCCGCATGGAAACTGCTTGCCGCAGAAGGCCGATCCGGTGGCGAGAG
CGAGCGAAGCCGCCCCGTCCACCGCTTCGATCGTCGCATCCTCGACACGATTTCGATGGACGGACGACAATTGGATGAAA
GGCCAGTCGCGACAGCAAAGACTGGAGGGGCCGATCTCCGTCTACGAGGTGCATGCCGGTTCCTGGCTGCGGGAGAATGG
CGGGCGGTCGCTCGACTGGGTCGAGCTCAGCCAGCGGCTCGTCCCCTATGTTCGCGAGATGGGATTCACCCATATCGAGC
TGCTGCCGATCATGGAGCACCCCTTCGGCGGCTCCTGGGGGTACCAACCGCTCGGCCTCTTCGCCCCGACCGGCCGTTAT
GGCACGCCTGAGGATCTCGCCTATTTCATCGACCGCTGCCATGGCGCCGGGATCGGCGTAATCCTCGACTGGGTGCCGGC
CCATTTTCCCACCGACGTCTGGGGGCTTGCCCGCTTCGACGGCACCGCGCTCTACGAACACGAAGACCCGCGCGAAGGCT
TTCATCGCGACTGGAACACGCTGATCTACAATCTCGGCCGCAACGAGGTGAAAGGCTTCCTGATCGCCAGCGCGCTTGAA
TGGCTCGAACGCTACCATATCGATGGATTGCGCGTCGATGCCGTCGCCTCGATGCTCTACCGCGACTACAGCCGCAACGA
GGGGGAGTGGATTCCGAACCGGTATGGCGGCCGTGAGAACCTGGAAGCGGTGGAATTCTTCAAGCACCTGAACAGTATCG
TCCACGAGCGCTGCCCGCATGCAATGATGATCGCCGAGGAATCGACGGCCTGGCCCGGCGTCACCAAGCCGCCGGAAGAG
GGTGGGCTCGGCTTTGACATGAAGTGGAACATGGGCTGGATGCATGACAGCCTGAGCTATATCGAGAAGGACCCCGTCTA
CCGGAGTTACCACCACGGCACGATGACCTTCGGGATGATCTATGCCTATTCCGAACGCTTCATCCTGCCGATTTCGCACG
ACGAGGTGGTCTATGGAAAGGGCTCGCTGCTTGGCAAGATGCCGGGCGACGAGTGGCAGAAATTCGCCAATCTGCGCAGC
TACCTCGCCTTCATGTGGGGCCATCCCGGCAAGAAGCTGATTTTCATGGGAGGCGAAATCGCCCAGCCGAGCGAGTGGAA
CCATGATGCGTCGATCGCCTGGGATGTGCTGGACCAGCCGGCGCATGCCGGGCTCCAGCGGCTGGTCAAGGATTTGAACG
GCTTTTACAAAGACGAGGCAGCCTTGCAGTTCGGCGATTTCCACTCCGAAGGCTTCGACTGGGCGGCGGCGGATGATGCC
GTCAACTCCGTTCTTGGCATGCTGCGTTACGCCCCCGATCGCTCGTCTTCGGTCCTTGTCGTCTCGAATTTCACGCCGGT
GCCGCGTTACGGCTACCGGATCGGCGTGCCGCAGGACGGCGTGTGGATCGAGAAGGTCACGACAGATGCGCGCGAATATG
GCGGCTCGGGCCTCGTCAACGGCGCAGTGTCGAGCGAATCCGTACCCGCGCACGGCAGGCCGCATTCGCTCTGGCTGACG
CTGCCTCCGCTGGCGACGGTCTTGCTCAAATCGCCTTGA

Upstream 100 bases:

>100_bases
CCTATGAAGTCGCCTATGAAGCCCGCAACAGGCCGAAGTGGCTGCCGATCCCGCTTGCCGGCCTTACCGAAATCGTATCG
CGCTTAGCGGGGGTAACGGC

Downstream 100 bases:

>100_bases
TAGGGCTCCACGTCCTGCCGGTCATTCGTGCGGAGCCGGATGGATGAAGGGCCAGGGGCTCGCCTCCGATCCCTTTTCGA
CGGGCACTTCGATGAGGACG

Product: glycogen branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase 2; Glycogen-branching enzyme 2; BE 2

Number of amino acids: Translated: 732; Mature: 732

Protein sequence:

>732_residues
MNVERSEFLAGVGHDALWALIEGRHGDPFSILGPHESGGMTIVRVYLPGAEGVDLIEAASGRVVTPFSIAHPSGLFAAAM
GSRMHYRLRITWPDGEQITEDPYSFGLLLGELDLHLISEGTHYSLSRTLGAVEMAIDDVAGVRFAVWAPNARRVSVVGDF
NAWDGRRNPMRLRQSAGVWELFMPRLAPGERYKFEIIDPHGNCLPQKADPVARASEAAPSTASIVASSTRFRWTDDNWMK
GQSRQQRLEGPISVYEVHAGSWLRENGGRSLDWVELSQRLVPYVREMGFTHIELLPIMEHPFGGSWGYQPLGLFAPTGRY
GTPEDLAYFIDRCHGAGIGVILDWVPAHFPTDVWGLARFDGTALYEHEDPREGFHRDWNTLIYNLGRNEVKGFLIASALE
WLERYHIDGLRVDAVASMLYRDYSRNEGEWIPNRYGGRENLEAVEFFKHLNSIVHERCPHAMMIAEESTAWPGVTKPPEE
GGLGFDMKWNMGWMHDSLSYIEKDPVYRSYHHGTMTFGMIYAYSERFILPISHDEVVYGKGSLLGKMPGDEWQKFANLRS
YLAFMWGHPGKKLIFMGGEIAQPSEWNHDASIAWDVLDQPAHAGLQRLVKDLNGFYKDEAALQFGDFHSEGFDWAAADDA
VNSVLGMLRYAPDRSSSVLVVSNFTPVPRYGYRIGVPQDGVWIEKVTTDAREYGGSGLVNGAVSSESVPAHGRPHSLWLT
LPPLATVLLKSP

Sequences:

>Translated_732_residues
MNVERSEFLAGVGHDALWALIEGRHGDPFSILGPHESGGMTIVRVYLPGAEGVDLIEAASGRVVTPFSIAHPSGLFAAAM
GSRMHYRLRITWPDGEQITEDPYSFGLLLGELDLHLISEGTHYSLSRTLGAVEMAIDDVAGVRFAVWAPNARRVSVVGDF
NAWDGRRNPMRLRQSAGVWELFMPRLAPGERYKFEIIDPHGNCLPQKADPVARASEAAPSTASIVASSTRFRWTDDNWMK
GQSRQQRLEGPISVYEVHAGSWLRENGGRSLDWVELSQRLVPYVREMGFTHIELLPIMEHPFGGSWGYQPLGLFAPTGRY
GTPEDLAYFIDRCHGAGIGVILDWVPAHFPTDVWGLARFDGTALYEHEDPREGFHRDWNTLIYNLGRNEVKGFLIASALE
WLERYHIDGLRVDAVASMLYRDYSRNEGEWIPNRYGGRENLEAVEFFKHLNSIVHERCPHAMMIAEESTAWPGVTKPPEE
GGLGFDMKWNMGWMHDSLSYIEKDPVYRSYHHGTMTFGMIYAYSERFILPISHDEVVYGKGSLLGKMPGDEWQKFANLRS
YLAFMWGHPGKKLIFMGGEIAQPSEWNHDASIAWDVLDQPAHAGLQRLVKDLNGFYKDEAALQFGDFHSEGFDWAAADDA
VNSVLGMLRYAPDRSSSVLVVSNFTPVPRYGYRIGVPQDGVWIEKVTTDAREYGGSGLVNGAVSSESVPAHGRPHSLWLT
LPPLATVLLKSP
>Mature_732_residues
MNVERSEFLAGVGHDALWALIEGRHGDPFSILGPHESGGMTIVRVYLPGAEGVDLIEAASGRVVTPFSIAHPSGLFAAAM
GSRMHYRLRITWPDGEQITEDPYSFGLLLGELDLHLISEGTHYSLSRTLGAVEMAIDDVAGVRFAVWAPNARRVSVVGDF
NAWDGRRNPMRLRQSAGVWELFMPRLAPGERYKFEIIDPHGNCLPQKADPVARASEAAPSTASIVASSTRFRWTDDNWMK
GQSRQQRLEGPISVYEVHAGSWLRENGGRSLDWVELSQRLVPYVREMGFTHIELLPIMEHPFGGSWGYQPLGLFAPTGRY
GTPEDLAYFIDRCHGAGIGVILDWVPAHFPTDVWGLARFDGTALYEHEDPREGFHRDWNTLIYNLGRNEVKGFLIASALE
WLERYHIDGLRVDAVASMLYRDYSRNEGEWIPNRYGGRENLEAVEFFKHLNSIVHERCPHAMMIAEESTAWPGVTKPPEE
GGLGFDMKWNMGWMHDSLSYIEKDPVYRSYHHGTMTFGMIYAYSERFILPISHDEVVYGKGSLLGKMPGDEWQKFANLRS
YLAFMWGHPGKKLIFMGGEIAQPSEWNHDASIAWDVLDQPAHAGLQRLVKDLNGFYKDEAALQFGDFHSEGFDWAAADDA
VNSVLGMLRYAPDRSSSVLVVSNFTPVPRYGYRIGVPQDGVWIEKVTTDAREYGGSGLVNGAVSSESVPAHGRPHSLWLT
LPPLATVLLKSP

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Homo sapiens, GI189458812, Length=638, Percent_Identity=27.4294670846395, Blast_Score=194, Evalue=2e-49,
Organism=Escherichia coli, GI1789839, Length=726, Percent_Identity=53.4435261707989, Blast_Score=771, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17554896, Length=360, Percent_Identity=29.4444444444444, Blast_Score=171, Evalue=9e-43,
Organism=Caenorhabditis elegans, GI32564391, Length=259, Percent_Identity=33.2046332046332, Blast_Score=154, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6320826, Length=647, Percent_Identity=25.6568778979907, Blast_Score=174, Evalue=6e-44,
Organism=Drosophila melanogaster, GI28573410, Length=642, Percent_Identity=27.2585669781931, Blast_Score=182, Evalue=5e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLGB2_RHIEC (Q2JZ21)

Other databases:

- EMBL:   CP000138
- RefSeq:   YP_472892.1
- ProteinModelPortal:   Q2JZ21
- SMR:   Q2JZ21
- STRING:   Q2JZ21
- GeneID:   3895849
- GenomeReviews:   CP000138_GR
- KEGG:   ret:RHE_PF00275
- eggNOG:   COG0296
- HOGENOM:   HBG287139
- OMA:   RVYHQNG
- PhylomeDB:   Q2JZ21
- ProtClustDB:   PRK05402
- HAMAP:   MF_00685
- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- Gene3D:   G3DSA:2.60.40.10
- TIGRFAMs:   TIGR01515

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48; SSF51445 Glyco_hydro_cat; SSF81296 Ig_E-set

EC number: =2.4.1.18

Molecular weight: Translated: 81931; Mature: 81931

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: ACT_SITE 308-308 ACT_SITE 343-343 ACT_SITE 348-348 ACT_SITE 411-411 ACT_SITE 413-413 ACT_SITE 466-466 ACT_SITE 533-533 ACT_SITE 534-534

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVERSEFLAGVGHDALWALIEGRHGDPFSILGPHESGGMTIVRVYLPGAEGVDLIEAAS
CCCCHHHHHHCCCCHHEEHEEECCCCCCCEEECCCCCCCEEEEEEEECCCCCCHHHHHCC
GRVVTPFSIAHPSGLFAAAMGSRMHYRLRITWPDGEQITEDPYSFGLLLGELDLHLISEG
CCEECEEECCCCCCHHHHHHCCCEEEEEEEECCCCCCCCCCCHHHEEEEEEEEEEEECCC
THYSLSRTLGAVEMAIDDVAGVRFAVWAPNARRVSVVGDFNAWDGRRNPMRLRQSAGVWE
CCEEHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCCCCCCCCCHHHHHHCCCHHH
LFMPRLAPGERYKFEIIDPHGNCLPQKADPVARASEAAPSTASIVASSTRFRWTDDNWMK
HHHCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHCCCCCCHHEEECCCEEEECCCCCCC
GQSRQQRLEGPISVYEVHAGSWLRENGGRSLDWVELSQRLVPYVREMGFTHIELLPIMEH
CCCHHHHCCCCCEEEEEECCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECC
PFGGSWGYQPLGLFAPTGRYGTPEDLAYFIDRCHGAGIGVILDWVPAHFPTDVWGLARFD
CCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHCCC
GTALYEHEDPREGFHRDWNTLIYNLGRNEVKGFLIASALEWLERYHIDGLRVDAVASMLY
CEEEECCCCHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHH
RDYSRNEGEWIPNRYGGRENLEAVEFFKHLNSIVHERCPHAMMIAEESTAWPGVTKPPEE
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCC
GGLGFDMKWNMGWMHDSLSYIEKDPVYRSYHHGTMTFGMIYAYSERFILPISHDEVVYGK
CCCCEEEECCCCCHHHHHHHHHCCCCHHHHCCCCEEEEEEEEECCCEEEEECCCCEEEEC
GSLLGKMPGDEWQKFANLRSYLAFMWGHPGKKLIFMGGEIAQPSEWNHDASIAWDVLDQP
CCEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCEEEEHHHCCC
AHAGLQRLVKDLNGFYKDEAALQFGDFHSEGFDWAAADDAVNSVLGMLRYAPDRSSSVLV
HHHHHHHHHHHHHHHHCCCHHEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEE
VSNFTPVPRYGYRIGVPQDGVWIEKVTTDAREYGGSGLVNGAVSSESVPAHGRPHSLWLT
EECCCCCCCCCEEECCCCCCEEEEEEHHHHHHHCCCCEEECCCCCCCCCCCCCCCEEEEE
LPPLATVLLKSP
CCCHHHHHCCCC
>Mature Secondary Structure
MNVERSEFLAGVGHDALWALIEGRHGDPFSILGPHESGGMTIVRVYLPGAEGVDLIEAAS
CCCCHHHHHHCCCCHHEEHEEECCCCCCCEEECCCCCCCEEEEEEEECCCCCCHHHHHCC
GRVVTPFSIAHPSGLFAAAMGSRMHYRLRITWPDGEQITEDPYSFGLLLGELDLHLISEG
CCEECEEECCCCCCHHHHHHCCCEEEEEEEECCCCCCCCCCCHHHEEEEEEEEEEEECCC
THYSLSRTLGAVEMAIDDVAGVRFAVWAPNARRVSVVGDFNAWDGRRNPMRLRQSAGVWE
CCEEHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCCCCCCCCCHHHHHHCCCHHH
LFMPRLAPGERYKFEIIDPHGNCLPQKADPVARASEAAPSTASIVASSTRFRWTDDNWMK
HHHCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHCCCCCCHHEEECCCEEEECCCCCCC
GQSRQQRLEGPISVYEVHAGSWLRENGGRSLDWVELSQRLVPYVREMGFTHIELLPIMEH
CCCHHHHCCCCCEEEEEECCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECC
PFGGSWGYQPLGLFAPTGRYGTPEDLAYFIDRCHGAGIGVILDWVPAHFPTDVWGLARFD
CCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHCCC
GTALYEHEDPREGFHRDWNTLIYNLGRNEVKGFLIASALEWLERYHIDGLRVDAVASMLY
CEEEECCCCHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHH
RDYSRNEGEWIPNRYGGRENLEAVEFFKHLNSIVHERCPHAMMIAEESTAWPGVTKPPEE
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCC
GGLGFDMKWNMGWMHDSLSYIEKDPVYRSYHHGTMTFGMIYAYSERFILPISHDEVVYGK
CCCCEEEECCCCCHHHHHHHHHCCCCHHHHCCCCEEEEEEEEECCCEEEEECCCCEEEEC
GSLLGKMPGDEWQKFANLRSYLAFMWGHPGKKLIFMGGEIAQPSEWNHDASIAWDVLDQP
CCEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCEEEEHHHCCC
AHAGLQRLVKDLNGFYKDEAALQFGDFHSEGFDWAAADDAVNSVLGMLRYAPDRSSSVLV
HHHHHHHHHHHHHHHHCCCHHEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEE
VSNFTPVPRYGYRIGVPQDGVWIEKVTTDAREYGGSGLVNGAVSSESVPAHGRPHSLWLT
EECCCCCCCCCEEECCCCCCEEEEEEHHHHHHHCCCCEEECCCCCCCCCCCCCCCEEEEE
LPPLATVLLKSP
CCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA