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The map label for this gene is tpiA

Identifier: 86360774

GI number: 86360774

Start: 40874

End: 41677

Strand: Reverse

Name: tpiA

Synonym: RHE_PF00040

Alternate gene names: 86360774

Gene position: 41677-40874 (Counterclockwise)

Preceding gene: 86360775

Following gene: 86360773

Centisome position: 6.49

GC content: 66.67

Gene sequence:

>804_bases
ATGGTCGTCTGGGTCGGCACCAGCTTCAAGATGAACAAGACGCTTGAGGAAGCGCTCGCCTTCGCGCGCCGCCTCGCCGA
TGCGGATCTTGAACGCGATCCCCGCGTGCAGCGCTTCGTCATCCCCTCCTTCACGGCGGTGCGCGAGGTCAAGCGTGTAC
TGACCGAAAGCTCGGTGAAGGTTGGCGCGCAGAACATGCATTGGGAGGATGCCGGCGCCTGGACCGGCGAGATTTCGCCG
CTGATGCTGAAGGACTGCCGGCTCGATCTCGTCGAGCTCGGCCATTCCGAGCGGCGCGAACATTTCGGCGAGACCGACGA
GACCGTCGGCCTCAAGGCTGCCGCCGCGATCCGCCACGGCCTGACGCCGCTGATCTGCATCGGCGAGACCCTGCAGGAAC
GCAACGAGGGCCGGGCCGACGCCGTGCTGCGGCGGCAGGTCGAGGCCGCCCTGCGGGGTGTCGACACCGAGGCCGGGGAG
GCTCCGATCCTGCTTGCCTACGAGCCGGTCTGGGCGATCGGCGTCAACGGCATTCCGGCGACGGCCGATTATGCCTCCGA
GCGGCACAGAGGGATCGCCGAAGTGGCGAAATCCATTCTCGGCCGCCCGGTGCCGGTTCTCTACGGCGGCAGCGTCAATC
CCGGCAATTGCGAGGAACTGATCGGCCAGCCCGATATCGACGGCCTGTTCATCGGCCGCTCCGCCTGGTCCGTCGAAGGC
TATCTCGACATTCTCGCCCGCGTCAGCGCGGCGATCGACCGCTCATCTCCACGCCAGACGGCGAGTGAAAGGAAACTGCC
ATGA

Upstream 100 bases:

>100_bases
GACTACCAGCCGACCAACGGTCTGCAGATCGCGCTGAAGGGCGGGCAGGTCGGCACGGATGGCTATTTCGCGCAGATCCG
CGACGGGAGGAAGAACTGAG

Downstream 100 bases:

>100_bases
AGATCGCAATTGGCGCCGACAGCGCCGGCAAACCGCTCCTGGATGTCATTGCCGCCCATCTCGCCGGCAAGGCGGATCTC
GAAGTGCACGATCTCAGCCA

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM 2; Triose-phosphate isomerase 2

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MVVWVGTSFKMNKTLEEALAFARRLADADLERDPRVQRFVIPSFTAVREVKRVLTESSVKVGAQNMHWEDAGAWTGEISP
LMLKDCRLDLVELGHSERREHFGETDETVGLKAAAAIRHGLTPLICIGETLQERNEGRADAVLRRQVEAALRGVDTEAGE
APILLAYEPVWAIGVNGIPATADYASERHRGIAEVAKSILGRPVPVLYGGSVNPGNCEELIGQPDIDGLFIGRSAWSVEG
YLDILARVSAAIDRSSPRQTASERKLP

Sequences:

>Translated_267_residues
MVVWVGTSFKMNKTLEEALAFARRLADADLERDPRVQRFVIPSFTAVREVKRVLTESSVKVGAQNMHWEDAGAWTGEISP
LMLKDCRLDLVELGHSERREHFGETDETVGLKAAAAIRHGLTPLICIGETLQERNEGRADAVLRRQVEAALRGVDTEAGE
APILLAYEPVWAIGVNGIPATADYASERHRGIAEVAKSILGRPVPVLYGGSVNPGNCEELIGQPDIDGLFIGRSAWSVEG
YLDILARVSAAIDRSSPRQTASERKLP
>Mature_267_residues
MVVWVGTSFKMNKTLEEALAFARRLADADLERDPRVQRFVIPSFTAVREVKRVLTESSVKVGAQNMHWEDAGAWTGEISP
LMLKDCRLDLVELGHSERREHFGETDETVGLKAAAAIRHGLTPLICIGETLQERNEGRADAVLRRQVEAALRGVDTEAGE
APILLAYEPVWAIGVNGIPATADYASERHRGIAEVAKSILGRPVPVLYGGSVNPGNCEELIGQPDIDGLFIGRSAWSVEG
YLDILARVSAAIDRSSPRQTASERKLP

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=246, Percent_Identity=38.6178861788618, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI226529917, Length=246, Percent_Identity=38.6178861788618, Blast_Score=142, Evalue=3e-34,
Organism=Escherichia coli, GI1790353, Length=215, Percent_Identity=39.0697674418605, Blast_Score=137, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17536593, Length=237, Percent_Identity=38.8185654008439, Blast_Score=130, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6320255, Length=246, Percent_Identity=34.5528455284553, Blast_Score=132, Evalue=6e-32,
Organism=Drosophila melanogaster, GI28572008, Length=245, Percent_Identity=38.3673469387755, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI28572006, Length=245, Percent_Identity=38.3673469387755, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI28572004, Length=245, Percent_Identity=37.9591836734694, Blast_Score=139, Evalue=2e-33,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS2_RHIEC (Q2JZQ2)

Other databases:

- EMBL:   CP000138
- RefSeq:   YP_472661.1
- ProteinModelPortal:   Q2JZQ2
- SMR:   Q2JZQ2
- STRING:   Q2JZQ2
- GeneID:   3896224
- GenomeReviews:   CP000138_GR
- KEGG:   ret:RHE_PF00040
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   AWTGEVS
- ProtClustDB:   PRK00042
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 29176; Mature: 29176

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 95-95 ACT_SITE 168-168 BINDING 10-10

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVWVGTSFKMNKTLEEALAFARRLADADLERDPRVQRFVIPSFTAVREVKRVLTESSVK
CEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHH
VGAQNMHWEDAGAWTGEISPLMLKDCRLDLVELGHSERREHFGETDETVGLKAAAAIRHG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHC
LTPLICIGETLQERNEGRADAVLRRQVEAALRGVDTEAGEAPILLAYEPVWAIGVNGIPA
CCCEEEHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCEEEECCCCCCC
TADYASERHRGIAEVAKSILGRPVPVLYGGSVNPGNCEELIGQPDIDGLFIGRSAWSVEG
CHHHHHHHCCCHHHHHHHHHCCCCCEEECCCCCCCCHHHHCCCCCCCEEEECCCCCCHHH
YLDILARVSAAIDRSSPRQTASERKLP
HHHHHHHHHHHHHCCCCHHHHHHCCCC
>Mature Secondary Structure
MVVWVGTSFKMNKTLEEALAFARRLADADLERDPRVQRFVIPSFTAVREVKRVLTESSVK
CEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHH
VGAQNMHWEDAGAWTGEISPLMLKDCRLDLVELGHSERREHFGETDETVGLKAAAAIRHG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHC
LTPLICIGETLQERNEGRADAVLRRQVEAALRGVDTEAGEAPILLAYEPVWAIGVNGIPA
CCCEEEHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCEEEECCCCCCC
TADYASERHRGIAEVAKSILGRPVPVLYGGSVNPGNCEELIGQPDIDGLFIGRSAWSVEG
CHHHHHHHCCCHHHHHHHHHCCCCCEEECCCCCCCCHHHHCCCCCCCEEEECCCCCCHHH
YLDILARVSAAIDRSSPRQTASERKLP
HHHHHHHHHHHHHCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA