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The map label for this gene is vatD [H]

Identifier: 85858232

GI number: 85858232

Start: 386859

End: 387449

Strand: Direct

Name: vatD [H]

Synonym: SYN_01140

Alternate gene names: 85858232

Gene position: 386859-387449 (Clockwise)

Preceding gene: 85858231

Following gene: 85858233

Centisome position: 12.17

GC content: 50.93

Gene sequence:

>591_bases
ATGAGGTCATCAACGATGAGAGGAAATACGGCAAGGTTCACATGGACCTTGTGTCAAATTCTCTGTGAGGCGCCTTACCG
GTTGACCATCATGCCCTGGAAGAAAGCCATGCTGGCGTCATGCGGGAAGAATGTCTTATTGCAGAGAGGATGCAAAATGA
CCTGGCAGAATGTTTATATTGGCGATGACGTCACTGTCGGTCCCAACGCTCTGTTTCTGTGCACCCGCGCCAGGATAAGA
ATTGGAAGCCACACCATGTTCGGACCCGGTGTGACGATGATCACCGGCGGCCATCGGATGGATGTTGCGGGACGTTATAT
GAATTCAATTACCAATGATGAAAAGCTGCCCGAAAATGACAGGGATATCGTTCTTGAAGGAGACAACTGGATCGGAGCGA
ATGCCACGATTCTGAAAGGGGTAACCATCGGAGAAGGGGCTGTGGTGGCTGCGGGAGCGGTGGTCACTAAGAATATACCG
GCATATTCCATCTGGGGCGGCGTTCCGGCTGAACGGATCGGCATGAGATTTAACGACGAAACGCTGTCAATTCATAAAGA
GTTGATGAAAAAAGCAGGGTTTCCGGGCTGA

Upstream 100 bases:

>100_bases
CCGGCATCCGTGAGAGAACGCATGTCCCGCTGGAATGTAAATAAAGTCAGGGATGCGTATTTAATCAACAGAGTCATGGA
ATGTCTGATCGGGATTTATG

Downstream 100 bases:

>100_bases
TGAATGATGACTGATCAGAAAACACTCAAGATTGTAGAAGACCTTTGCGGCGCGCTGGCGGAAGAAAAAGTCGATTATTG
CCACTGGAAAAGCAACGAGG

Product: acetyltransferase

Products: CoA; acetylmaltose [C]

Alternate protein names: Virginiamycin acetyltransferase D; Vat(D) [H]

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MRSSTMRGNTARFTWTLCQILCEAPYRLTIMPWKKAMLASCGKNVLLQRGCKMTWQNVYIGDDVTVGPNALFLCTRARIR
IGSHTMFGPGVTMITGGHRMDVAGRYMNSITNDEKLPENDRDIVLEGDNWIGANATILKGVTIGEGAVVAAGAVVTKNIP
AYSIWGGVPAERIGMRFNDETLSIHKELMKKAGFPG

Sequences:

>Translated_196_residues
MRSSTMRGNTARFTWTLCQILCEAPYRLTIMPWKKAMLASCGKNVLLQRGCKMTWQNVYIGDDVTVGPNALFLCTRARIR
IGSHTMFGPGVTMITGGHRMDVAGRYMNSITNDEKLPENDRDIVLEGDNWIGANATILKGVTIGEGAVVAAGAVVTKNIP
AYSIWGGVPAERIGMRFNDETLSIHKELMKKAGFPG
>Mature_196_residues
MRSSTMRGNTARFTWTLCQILCEAPYRLTIMPWKKAMLASCGKNVLLQRGCKMTWQNVYIGDDVTVGPNALFLCTRARIR
IGSHTMFGPGVTMITGGHRMDVAGRYMNSITNDEKLPENDRDIVLEGDNWIGANATILKGVTIGEGAVVAAGAVVTKNIP
AYSIWGGVPAERIGMRFNDETLSIHKELMKKAGFPG

Specific function: Inactivates the A compounds of streptogramin antibiotics by acetylation, thus providing resistance to these antibiotics [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786664, Length=117, Percent_Identity=35.042735042735, Blast_Score=64, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6322243, Length=141, Percent_Identity=32.6241134751773, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00132 Hexapep [H]

EC number: 2.3.1.79 [C]

Molecular weight: Translated: 21467; Mature: 21467

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
8.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
8.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSSTMRGNTARFTWTLCQILCEAPYRLTIMPWKKAMLASCGKNVLLQRGCKMTWQNVYI
CCCCCCCCCCEEHHHHHHHHHHCCCCEEEECCHHHHHHHHCCHHHHHHCCCCCEEEEEEE
GDDVTVGPNALFLCTRARIRIGSHTMFGPGVTMITGGHRMDVAGRYMNSITNDEKLPEND
CCCCEECCCEEEEEEECEEEECCEEEECCCEEEEECCCEECHHHHHHHHCCCCCCCCCCC
RDIVLEGDNWIGANATILKGVTIGEGAVVAAGAVVTKNIPAYSIWGGVPAERIGMRFNDE
CEEEEECCCEECCCCEEEEEEEECCCCEEEECEEEECCCCCEEECCCCCHHHHCCEECCH
TLSIHKELMKKAGFPG
HHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRSSTMRGNTARFTWTLCQILCEAPYRLTIMPWKKAMLASCGKNVLLQRGCKMTWQNVYI
CCCCCCCCCCEEHHHHHHHHHHCCCCEEEECCHHHHHHHHCCHHHHHHCCCCCEEEEEEE
GDDVTVGPNALFLCTRARIRIGSHTMFGPGVTMITGGHRMDVAGRYMNSITNDEKLPEND
CCCCEECCCEEEEEEECEEEECCEEEECCCEEEEECCCEECHHHHHHHHCCCCCCCCCCC
RDIVLEGDNWIGANATILKGVTIGEGAVVAAGAVVTKNIPAYSIWGGVPAERIGMRFNDE
CEEEEECCCEECCCCEEEEEEEECCCCEEEECEEEECCCCCEEECCCCCHHHHCCEECCH
TLSIHKELMKKAGFPG
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 0.20 {glucose} 0.11 {maltose}

Km value (mM): 62 {glucose}} 90 {maltose}} 0.018 {acetyl-CoA}} 40 {triethanolamine}} [C]

Substrates: Acetyl-CoA; maltose [C]

Specific reaction: Acetyl-CoA + maltose = CoA + acetylmaltose [C]

General reaction: Acyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8257133 [H]