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The map label for this gene is traD [H]

Identifier: 85375765

GI number: 85375765

Start: 2956825

End: 2959080

Strand: Direct

Name: traD [H]

Synonym: ELI_14690

Alternate gene names: 85375765

Gene position: 2956825-2959080 (Clockwise)

Preceding gene: 85375764

Following gene: 85375766

Centisome position: 96.87

GC content: 58.42

Gene sequence:

>2256_bases
ATGTTTGCAGCAGGCCTCAAAGGCCCTGTGATCGTCACCGCGCTAGTCGTTATGGGTACCTGTTATTGGGAAGTGAGAAC
GACTCTGACCGATCACCAGTTCTACCTGATCTGGATGCATATCTACGCGAGCCTTTACGCCTTCATGGAGTTCGATCCGG
CAAAACTGGTCAACCTCACGCTGGCCGATGGAGACAAGGTTGGTCTCCCTTTTGCGGTGGTTCGCGAGTTCCCACCCATG
CGCGATGCAGTCGACGCGTTCGTGTCCGCGACCAAGCAAGGATTGCTCATGTCAGCTGTCTTTCTTGCGCCAGCATTTGT
GTTCTTCTGGTGGTTCGCCGAGCGCTTCGGTGGCCGCTCGAAAGAACGCAAGCACGAGCGCGGAGCCATGCTCGTTTCAC
TCGATGAACTCGAGGATGAAATCGAGCGCTATAATGGCAAGTATCGCGCTGAAGAGCTGAGCCGGAAGTTCGGTTGGAAA
TGGCGCCTTGCAAGCTCTTCTGCGCTGGCCGAAGCCGGGCATTATGCGCCCGCCCATCTTGCTGGCGTAAGCTGGCCATG
GCGGCTTGAGCAGAGCCATACCATGCTGATTGGGACGACCGGAACCGGTAAGACCGTCGCGCTTACAGAACTCGTGACCG
AGGCCCGTCAGCGCGGCCAACGCGCCGTGATTTTCGATCTCACCGGTGCCTTTATCGAGGCCTACTACGATCCGGCACGT
GACGTTATTCTCAACCCAATCGATGCGCGTTGTCCGGTGTGGAGCGTTTTCAATGACTGCTCGAGCGAAGCCGAATTCCA
TGCCGCAGCCGAGGCGCTTGTGCCTCATGATGGCGGCGGATCGGAACAGTTCTGGGTGCTTGCTGCACGGATGTTGTTTG
TCGAGATGTGTCTCCATCTCTCGCGCAAAGGGGCCGCGACCAACGAGGCTCTGGCCCAGAAATTGATGACCGCGGATCTG
TCCGAGGTGCACAAGCTGATGCGCGGAACAATGGCCGATCCGCTCACCGCTCCGGAAGCTGCACGTATGGCAGAATCGAT
CCGTGCGGTGTTCAACGCCAACGCCAAAGTGCTCAAGCTTCTGCCCTCGGAGGGACCACGCTTTTCGGTCCGCGACTGGG
TTAAGGGCGACTGCGAACCGGGTTCGATCCTGTTTCTTTCGGCGCGCTATGTCGACATGAGCATCTCCTCGCAATTGCTC
ACTTTATGGCTCGATACAGCGATGAACACGCTGATGACACTCGAGCGCACGCAAGACCTCAGGATGTGGTTCCTGATTGA
CGAGCTTGGTGCCCTTCATCGTCTGCCAGCGCTCGAGAAAGGGCTGCAGACCGCGCGCAATTTCGGTGGTGCGATCGTCA
CCGGCGTCCATGCCTTTGCCAAACTCAAGGAAGTCTACGGCGAGAACATGGCCATGACACTCTCGTCGCTTGCGCGGACC
AAGCTGATCCTCGCAACAGCCGACCGCGAGACAGCAACATGGTGCTCCGATTTCATCGGTCACCGGCAGGTGCGCGACAT
GGAAGAGGGCTATAGTTACGGCTACAACAATGCCCGCGACGCGGTCAGCCTGACACCGAGGCGGCAGGTGGAGCCTCTGC
TTCTTCCCGACCAGTTTATGAATCTTCCAAGGTTGACGGCTTACATCAAGTTTCCTGACGGTTTCCCTGCCGCCCCGGTT
TCGCTGATACCTCGTTCGCGCGAGCGTATTGCCGAAGGTTTCATACCCCGAGAGACGCCGCCAGTTAATGGCGCACAGGA
TCCTGACACGGCTTCAGCGACGGGGCGAATGGCAAGCCCTTTGACGGATGGAGAGCATCCTGCCTCGAATGATGACGGTG
CTGGCAAACCCGTCGATCCGAAGCAGCTAAAGCTGACGTTGCCAACGCCGGCTAGAGCCGCTGACGAAGCGCGAAATGAT
CGGCAAGCGGCCGCGCGCGATCAGTTGGAAGCGTCCCGTCAGTCAGATCAGACCAAGGCTGAAACAAGCGTCGATGCGGT
CGGTCCCAAGGCCCCGACTCTGCCACGTTCGACCGTGCAAACGAGCGAGGATGCCGCAGCCTCGAATGTCGCGCAGGACA
AAAAGGAAGCGGGAACTGAAGTCCCGAAGGACCATGGCAAAGATGCGCTTTCGCTTGAAAACAGATCATCCGCGACCGGT
GCCAAACCAATCGATCCTGCCGAAAAGGACCTTCGTGAAGGCGCTGTCAGCGATCCGCCCGCAAAGGACTTGGGTGAGTT
TGAGCCCGACATGTGA

Upstream 100 bases:

>100_bases
CTCGGTCTAGCTTTGAGCACCAACATGGGAGGCCATTCCCATGAAGCATAACCTCCTTAACTTCACCCGCGGAAGCCAGT
TGCTAGGTCACTTTGCCTTC

Downstream 100 bases:

>100_bases
TGCGAGACTTGCGGAGGTTTTTAGGTGCCAGAGGGGGAGCCATGACGAGGATCAGAGACGATGCTTTCCGTTGCCAATGT
GCGCTCCCCTTCAGCTGCTG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 751; Mature: 751

Protein sequence:

>751_residues
MFAAGLKGPVIVTALVVMGTCYWEVRTTLTDHQFYLIWMHIYASLYAFMEFDPAKLVNLTLADGDKVGLPFAVVREFPPM
RDAVDAFVSATKQGLLMSAVFLAPAFVFFWWFAERFGGRSKERKHERGAMLVSLDELEDEIERYNGKYRAEELSRKFGWK
WRLASSSALAEAGHYAPAHLAGVSWPWRLEQSHTMLIGTTGTGKTVALTELVTEARQRGQRAVIFDLTGAFIEAYYDPAR
DVILNPIDARCPVWSVFNDCSSEAEFHAAAEALVPHDGGGSEQFWVLAARMLFVEMCLHLSRKGAATNEALAQKLMTADL
SEVHKLMRGTMADPLTAPEAARMAESIRAVFNANAKVLKLLPSEGPRFSVRDWVKGDCEPGSILFLSARYVDMSISSQLL
TLWLDTAMNTLMTLERTQDLRMWFLIDELGALHRLPALEKGLQTARNFGGAIVTGVHAFAKLKEVYGENMAMTLSSLART
KLILATADRETATWCSDFIGHRQVRDMEEGYSYGYNNARDAVSLTPRRQVEPLLLPDQFMNLPRLTAYIKFPDGFPAAPV
SLIPRSRERIAEGFIPRETPPVNGAQDPDTASATGRMASPLTDGEHPASNDDGAGKPVDPKQLKLTLPTPARAADEARND
RQAAARDQLEASRQSDQTKAETSVDAVGPKAPTLPRSTVQTSEDAAASNVAQDKKEAGTEVPKDHGKDALSLENRSSATG
AKPIDPAEKDLREGAVSDPPAKDLGEFEPDM

Sequences:

>Translated_751_residues
MFAAGLKGPVIVTALVVMGTCYWEVRTTLTDHQFYLIWMHIYASLYAFMEFDPAKLVNLTLADGDKVGLPFAVVREFPPM
RDAVDAFVSATKQGLLMSAVFLAPAFVFFWWFAERFGGRSKERKHERGAMLVSLDELEDEIERYNGKYRAEELSRKFGWK
WRLASSSALAEAGHYAPAHLAGVSWPWRLEQSHTMLIGTTGTGKTVALTELVTEARQRGQRAVIFDLTGAFIEAYYDPAR
DVILNPIDARCPVWSVFNDCSSEAEFHAAAEALVPHDGGGSEQFWVLAARMLFVEMCLHLSRKGAATNEALAQKLMTADL
SEVHKLMRGTMADPLTAPEAARMAESIRAVFNANAKVLKLLPSEGPRFSVRDWVKGDCEPGSILFLSARYVDMSISSQLL
TLWLDTAMNTLMTLERTQDLRMWFLIDELGALHRLPALEKGLQTARNFGGAIVTGVHAFAKLKEVYGENMAMTLSSLART
KLILATADRETATWCSDFIGHRQVRDMEEGYSYGYNNARDAVSLTPRRQVEPLLLPDQFMNLPRLTAYIKFPDGFPAAPV
SLIPRSRERIAEGFIPRETPPVNGAQDPDTASATGRMASPLTDGEHPASNDDGAGKPVDPKQLKLTLPTPARAADEARND
RQAAARDQLEASRQSDQTKAETSVDAVGPKAPTLPRSTVQTSEDAAASNVAQDKKEAGTEVPKDHGKDALSLENRSSATG
AKPIDPAEKDLREGAVSDPPAKDLGEFEPDM
>Mature_751_residues
MFAAGLKGPVIVTALVVMGTCYWEVRTTLTDHQFYLIWMHIYASLYAFMEFDPAKLVNLTLADGDKVGLPFAVVREFPPM
RDAVDAFVSATKQGLLMSAVFLAPAFVFFWWFAERFGGRSKERKHERGAMLVSLDELEDEIERYNGKYRAEELSRKFGWK
WRLASSSALAEAGHYAPAHLAGVSWPWRLEQSHTMLIGTTGTGKTVALTELVTEARQRGQRAVIFDLTGAFIEAYYDPAR
DVILNPIDARCPVWSVFNDCSSEAEFHAAAEALVPHDGGGSEQFWVLAARMLFVEMCLHLSRKGAATNEALAQKLMTADL
SEVHKLMRGTMADPLTAPEAARMAESIRAVFNANAKVLKLLPSEGPRFSVRDWVKGDCEPGSILFLSARYVDMSISSQLL
TLWLDTAMNTLMTLERTQDLRMWFLIDELGALHRLPALEKGLQTARNFGGAIVTGVHAFAKLKEVYGENMAMTLSSLART
KLILATADRETATWCSDFIGHRQVRDMEEGYSYGYNNARDAVSLTPRRQVEPLLLPDQFMNLPRLTAYIKFPDGFPAAPV
SLIPRSRERIAEGFIPRETPPVNGAQDPDTASATGRMASPLTDGEHPASNDDGAGKPVDPKQLKLTLPTPARAADEARND
RQAAARDQLEASRQSDQTKAETSVDAVGPKAPTLPRSTVQTSEDAAASNVAQDKKEAGTEVPKDHGKDALSLENRSSATG
AKPIDPAEKDLREGAVSDPPAKDLGEFEPDM

Specific function: TraD is a cell envelope protein. The function of traD is unknown but it is essential for DNA transfer. It may form or may be part of a non-specific pore for DNA export, or it may directly energizes DNA transport [H]

COG id: COG3505

COG function: function code U; Type IV secretory pathway, VirD4 components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To traD of plasmid F [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014128
- InterPro:   IPR019476
- InterPro:   IPR022585 [H]

Pfam domain/function: PF12615 TraD_N; PF10412 TrwB_AAD_bind [H]

EC number: NA

Molecular weight: Translated: 82598; Mature: 82598

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFAAGLKGPVIVTALVVMGTCYWEVRTTLTDHQFYLIWMHIYASLYAFMEFDPAKLVNLT
CCCCCCCCHHHHHHHHHHHHHHHEEEHEECCCHHHHHHHHHHHHHHHHHCCCCHHEEEEE
LADGDKVGLPFAVVREFPPMRDAVDAFVSATKQGLLMSAVFLAPAFVFFWWFAERFGGRS
EECCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KERKHERGAMLVSLDELEDEIERYNGKYRAEELSRKFGWKWRLASSSALAEAGHYAPAHL
HHHHHHCCCEEEEHHHHHHHHHHHCCCHHHHHHHHHCCCEEEECCCHHHHHCCCCCCHHH
AGVSWPWRLEQSHTMLIGTTGTGKTVALTELVTEARQRGQRAVIFDLTGAFIEAYYDPAR
CCCCCCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHCCHH
DVILNPIDARCPVWSVFNDCSSEAEFHAAAEALVPHDGGGSEQFWVLAARMLFVEMCLHL
HHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
SRKGAATNEALAQKLMTADLSEVHKLMRGTMADPLTAPEAARMAESIRAVFNANAKVLKL
HHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEE
LPSEGPRFSVRDWVKGDCEPGSILFLSARYVDMSISSQLLTLWLDTAMNTLMTLERTQDL
ECCCCCCCCHHHHHCCCCCCCCEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMWFLIDELGALHRLPALEKGLQTARNFGGAIVTGVHAFAKLKEVYGENMAMTLSSLART
HHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
KLILATADRETATWCSDFIGHRQVRDMEEGYSYGYNNARDAVSLTPRRQVEPLLLPDQFM
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCCHHHC
NLPRLTAYIKFPDGFPAAPVSLIPRSRERIAEGFIPRETPPVNGAQDPDTASATGRMASP
CCCCEEEEEECCCCCCCCCHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
LTDGEHPASNDDGAGKPVDPKQLKLTLPTPARAADEARNDRQAAARDQLEASRQSDQTKA
CCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
ETSVDAVGPKAPTLPRSTVQTSEDAAASNVAQDKKEAGTEVPKDHGKDALSLENRSSATG
HHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCC
AKPIDPAEKDLREGAVSDPPAKDLGEFEPDM
CCCCCHHHHHHHCCCCCCCCHHHHCCCCCCC
>Mature Secondary Structure
MFAAGLKGPVIVTALVVMGTCYWEVRTTLTDHQFYLIWMHIYASLYAFMEFDPAKLVNLT
CCCCCCCCHHHHHHHHHHHHHHHEEEHEECCCHHHHHHHHHHHHHHHHHCCCCHHEEEEE
LADGDKVGLPFAVVREFPPMRDAVDAFVSATKQGLLMSAVFLAPAFVFFWWFAERFGGRS
EECCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KERKHERGAMLVSLDELEDEIERYNGKYRAEELSRKFGWKWRLASSSALAEAGHYAPAHL
HHHHHHCCCEEEEHHHHHHHHHHHCCCHHHHHHHHHCCCEEEECCCHHHHHCCCCCCHHH
AGVSWPWRLEQSHTMLIGTTGTGKTVALTELVTEARQRGQRAVIFDLTGAFIEAYYDPAR
CCCCCCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHCCHH
DVILNPIDARCPVWSVFNDCSSEAEFHAAAEALVPHDGGGSEQFWVLAARMLFVEMCLHL
HHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
SRKGAATNEALAQKLMTADLSEVHKLMRGTMADPLTAPEAARMAESIRAVFNANAKVLKL
HHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEE
LPSEGPRFSVRDWVKGDCEPGSILFLSARYVDMSISSQLLTLWLDTAMNTLMTLERTQDL
ECCCCCCCCHHHHHCCCCCCCCEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMWFLIDELGALHRLPALEKGLQTARNFGGAIVTGVHAFAKLKEVYGENMAMTLSSLART
HHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
KLILATADRETATWCSDFIGHRQVRDMEEGYSYGYNNARDAVSLTPRRQVEPLLLPDQFM
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCCHHHC
NLPRLTAYIKFPDGFPAAPVSLIPRSRERIAEGFIPRETPPVNGAQDPDTASATGRMASP
CCCCEEEEEECCCCCCCCCHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
LTDGEHPASNDDGAGKPVDPKQLKLTLPTPARAADEARNDRQAAARDQLEASRQSDQTKA
CCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
ETSVDAVGPKAPTLPRSTVQTSEDAAASNVAQDKKEAGTEVPKDHGKDALSLENRSSATG
HHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCC
AKPIDPAEKDLREGAVSDPPAKDLGEFEPDM
CCCCCHHHHHHHCCCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2164585 [H]