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The map label for this gene is gltX

Identifier: 85375723

GI number: 85375723

Start: 2917404

End: 2918684

Strand: Direct

Name: gltX

Synonym: ELI_14480

Alternate gene names: 85375723

Gene position: 2917404-2918684 (Clockwise)

Preceding gene: 85375722

Following gene: 85375725

Centisome position: 95.58

GC content: 64.4

Gene sequence:

>1281_bases
ATGCTTGCCAAGAAACATGGCGGACGCTTCCTGCTGCGCATCGACGATACCGATGCCGAGCGGTCGAAAGAGGAATATGT
CCACGCGATCCGGGCCGATCTGGCGTGGCTCGGGTTGGAGCCGGATGGCGAGGAGCGTCAATCGGAACGGCTGGAACATT
ACGAGGCGGCGTTTGAAGCGCTGAAAGCGGCGGGGCGGGTCTACCCGGCCTACGAGACCGCGCAGGAGCTGGAGCTCAAG
CGCAAAATCCAGCTGGGTCGCGGGCTACCGCCAATCTACGACCGCGCGGCGCTGAAGCTGAGCGACGACGAACGCGCAGC
GAAGGAAGCGCAGGGTATTGCACCGCACTGGCGCTTCAAGCTCGACCACGACGTGCCGATCACGTGGGAGGACCGCGTGC
GCGGGCCGCAAAAGTTCGATCCCGCACAGCTCTCCGATCCGGTCATCCGCCGCGCCGACGGCAGCTGGCTCTACATGTTG
CCGAGCGCTGTCGACGATATCGACATGGGCATCACCCATGTGCTGCGCGGCGAAGACCATGTCTCCAACACCGCCGTGCA
AATTCAGATGTTTACCGCGCTTTTTGCTGCGCAACATGACGCGCAACAAAGCCCACCGGAATTCGCGCACGAGGCGCTGC
TGGTCGGCAAGGAAGGCAAGCTGTCAAAGCGGCTCGGCTCGCTCGGCTGCGATGCTTTCCGCGAGCGCGGGATCGAGCCC
GAGGCGCTGGTCGCGATGCTGGCACGGCTCGGCACGTCGCAGCCGGTGGAGCCGATTGCCGACCGACAGGTACTGCTCGA
TACGTTTGATCTCTCGACCTTCGGGCGCGCGCCCGCCAAGTTCGACGATGCCGAGCTTGAGCGGGTCAATACCGCCATCG
TTCACGCAATGGACTTCGAGCAGGTGAAGCAACGCTTGCCCGAAGGTATCGACGCCGCAGGCTGGCATGCGATCCAGCCC
AACCTCGCGACTGTCGACGAAGCGGGCGAATGGTGGCGGCTGGTGACAGGGCCGATCGAGCAGCCCGAGTTCTCCGATGA
AGATCGCGCCTATCTCGCCGAAGCCGCGGAGACGCTCGCTTGGGACGACGATCCATGGGGCACTCTGACCGCAAAGCTCA
AGGATAGCACCGGCCGCAAGGGGAAGGCGCTATTCCTGCCGCTCCGCCAGGCGCTCACCGGCATGAACCACGGACCCGAC
ATGGGCGAGCTGCTTCCACTGATTGGAGAGGAAGAAGCGCGGGCGCGGTTGCAGAGCGCCGCCGATCAACGCGGCGGTTA
A

Upstream 100 bases:

>100_bases
CCGCTCGGCTAGGGCGCTGCGCATGACCACGACACGTTTCGCCCCCTCTCCGACCGGCCGCCTACACGTCGGGAATATCC
GCACCGCTCTGCACAACTGG

Downstream 100 bases:

>100_bases
GTATTCGAGAGTCTGCGCGGGAAGTGACAGCGCGGCTCTGCCGTCATCCGCCGTGCTTCTCCAGCTCGTCGCCGGCCAGC
GTCACGACGTGCAGCAGATT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 2; GluRS 2

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MLAKKHGGRFLLRIDDTDAERSKEEYVHAIRADLAWLGLEPDGEERQSERLEHYEAAFEALKAAGRVYPAYETAQELELK
RKIQLGRGLPPIYDRAALKLSDDERAAKEAQGIAPHWRFKLDHDVPITWEDRVRGPQKFDPAQLSDPVIRRADGSWLYML
PSAVDDIDMGITHVLRGEDHVSNTAVQIQMFTALFAAQHDAQQSPPEFAHEALLVGKEGKLSKRLGSLGCDAFRERGIEP
EALVAMLARLGTSQPVEPIADRQVLLDTFDLSTFGRAPAKFDDAELERVNTAIVHAMDFEQVKQRLPEGIDAAGWHAIQP
NLATVDEAGEWWRLVTGPIEQPEFSDEDRAYLAEAAETLAWDDDPWGTLTAKLKDSTGRKGKALFLPLRQALTGMNHGPD
MGELLPLIGEEEARARLQSAADQRGG

Sequences:

>Translated_426_residues
MLAKKHGGRFLLRIDDTDAERSKEEYVHAIRADLAWLGLEPDGEERQSERLEHYEAAFEALKAAGRVYPAYETAQELELK
RKIQLGRGLPPIYDRAALKLSDDERAAKEAQGIAPHWRFKLDHDVPITWEDRVRGPQKFDPAQLSDPVIRRADGSWLYML
PSAVDDIDMGITHVLRGEDHVSNTAVQIQMFTALFAAQHDAQQSPPEFAHEALLVGKEGKLSKRLGSLGCDAFRERGIEP
EALVAMLARLGTSQPVEPIADRQVLLDTFDLSTFGRAPAKFDDAELERVNTAIVHAMDFEQVKQRLPEGIDAAGWHAIQP
NLATVDEAGEWWRLVTGPIEQPEFSDEDRAYLAEAAETLAWDDDPWGTLTAKLKDSTGRKGKALFLPLRQALTGMNHGPD
MGELLPLIGEEEARARLQSAADQRGG
>Mature_426_residues
MLAKKHGGRFLLRIDDTDAERSKEEYVHAIRADLAWLGLEPDGEERQSERLEHYEAAFEALKAAGRVYPAYETAQELELK
RKIQLGRGLPPIYDRAALKLSDDERAAKEAQGIAPHWRFKLDHDVPITWEDRVRGPQKFDPAQLSDPVIRRADGSWLYML
PSAVDDIDMGITHVLRGEDHVSNTAVQIQMFTALFAAQHDAQQSPPEFAHEALLVGKEGKLSKRLGSLGCDAFRERGIEP
EALVAMLARLGTSQPVEPIADRQVLLDTFDLSTFGRAPAKFDDAELERVNTAIVHAMDFEQVKQRLPEGIDAAGWHAIQP
NLATVDEAGEWWRLVTGPIEQPEFSDEDRAYLAEAAETLAWDDDPWGTLTAKLKDSTGRKGKALFLPLRQALTGMNHGPD
MGELLPLIGEEEARARLQSAADQRGG

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=474, Percent_Identity=28.4810126582279, Blast_Score=146, Evalue=4e-35,
Organism=Escherichia coli, GI1788743, Length=326, Percent_Identity=35.5828220858896, Blast_Score=184, Evalue=8e-48,
Organism=Caenorhabditis elegans, GI71993394, Length=457, Percent_Identity=27.1334792122538, Blast_Score=127, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6324540, Length=471, Percent_Identity=26.1146496815287, Blast_Score=119, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24665321, Length=466, Percent_Identity=28.9699570815451, Blast_Score=140, Evalue=2e-33,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE2_ERYLH (Q2N5Q3)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_459785.1
- STRING:   Q2N5Q3
- GeneID:   3871196
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_14480
- NMPDR:   fig|314225.3.peg.123
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- ProtClustDB:   PRK12558
- BioCyc:   ELIT314225:ELI_14480-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 47444; Mature: 47444

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 249-249

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKKHGGRFLLRIDDTDAERSKEEYVHAIRADLAWLGLEPDGEERQSERLEHYEAAFEA
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHH
LKAAGRVYPAYETAQELELKRKIQLGRGLPPIYDRAALKLSDDERAAKEAQGIAPHWRFK
HHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHCCCEEEECCHHHHHHHHHCCCCCEEEE
LDHDVPITWEDRVRGPQKFDPAQLSDPVIRRADGSWLYMLPSAVDDIDMGITHVLRGEDH
ECCCCCCCCHHHCCCCCCCCCCCCCCHHHHCCCCCEEEECCCHHHHHHHHHHHHHCCCCC
VSNTAVQIQMFTALFAAQHDAQQSPPEFAHEALLVGKEGKLSKRLGSLGCDAFRERGIEP
CCCCEEEHHHHHHHHHHHHCCCCCCHHHHHHHHEECCCCCHHHHHHHCCHHHHHHCCCCH
EALVAMLARLGTSQPVEPIADRQVLLDTFDLSTFGRAPAKFDDAELERVNTAIVHAMDFE
HHHHHHHHHHCCCCCCCHHHCCHHHHHHHCCHHCCCCCCCCCHHHHHHHHHHHHHHHCHH
QVKQRLPEGIDAAGWHAIQPNLATVDEAGEWWRLVTGPIEQPEFSDEDRAYLAEAAETLA
HHHHHCCCCCCCCCCEECCCCCCCHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHC
WDDDPWGTLTAKLKDSTGRKGKALFLPLRQALTGMNHGPDMGELLPLIGEEEARARLQSA
CCCCCCCEEEEEEECCCCCCCCEEEEEHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHH
ADQRGG
HHHCCC
>Mature Secondary Structure
MLAKKHGGRFLLRIDDTDAERSKEEYVHAIRADLAWLGLEPDGEERQSERLEHYEAAFEA
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHH
LKAAGRVYPAYETAQELELKRKIQLGRGLPPIYDRAALKLSDDERAAKEAQGIAPHWRFK
HHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHCCCEEEECCHHHHHHHHHCCCCCEEEE
LDHDVPITWEDRVRGPQKFDPAQLSDPVIRRADGSWLYMLPSAVDDIDMGITHVLRGEDH
ECCCCCCCCHHHCCCCCCCCCCCCCCHHHHCCCCCEEEECCCHHHHHHHHHHHHHCCCCC
VSNTAVQIQMFTALFAAQHDAQQSPPEFAHEALLVGKEGKLSKRLGSLGCDAFRERGIEP
CCCCEEEHHHHHHHHHHHHCCCCCCHHHHHHHHEECCCCCHHHHHHHCCHHHHHHCCCCH
EALVAMLARLGTSQPVEPIADRQVLLDTFDLSTFGRAPAKFDDAELERVNTAIVHAMDFE
HHHHHHHHHHCCCCCCCHHHCCHHHHHHHCCHHCCCCCCCCCHHHHHHHHHHHHHHHCHH
QVKQRLPEGIDAAGWHAIQPNLATVDEAGEWWRLVTGPIEQPEFSDEDRAYLAEAAETLA
HHHHHCCCCCCCCCCEECCCCCCCHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHC
WDDDPWGTLTAKLKDSTGRKGKALFLPLRQALTGMNHGPDMGELLPLIGEEEARARLQSA
CCCCCCCEEEEEEECCCCCCCCEEEEEHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHH
ADQRGG
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA