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The map label for this gene is mscS [H]

Identifier: 85375709

GI number: 85375709

Start: 2899061

End: 2899894

Strand: Direct

Name: mscS [H]

Synonym: ELI_14410

Alternate gene names: 85375709

Gene position: 2899061-2899894 (Clockwise)

Preceding gene: 85375706

Following gene: 85375713

Centisome position: 94.98

GC content: 63.19

Gene sequence:

>834_bases
ATGACTGCCGAAGCCGATATCGCCGCCCTGACCATCCCCGAACAAATTCTGCTGTGGCTGCGCCTCAACCTGATCGAAAT
TGCCGGGGCCTTCGCGGTTCTGTTGATCGGCCTGTTCCTCGCCGGGCTGCTGTCGCGCGCGTTCGAACGGGCCCTGGCCC
GCTCGCCGCGGTTCGATCCGACGGTCGCCAATTTCCTCTCCAACGTCGTCAAATACGCGTTGTGGGCCCTCGTGCTCGTC
ACCGTGCTGGCGCAGTTCGGGGTGGAGACTACCAGCATCCTTGCCGCGCTCGGCGGCATGGCGCTGGCCATCGGGCTCGC
GTTGCAGGGCACGCTCAGCAATGTCGCGTCGGGGGTGATGATCCTGGTGCAGAAACCGTTCAAGGTGGGCGAGGCGATCA
ATGTCGGCTCGATTACCGCGGTGGTGCAGAATATCGGCCTGTTCACCACCGAGCTCAAGCAATTCGACGGGCTGTTCGTG
ATGATCCCGAACTCCGAGCTCTGGAACAAGCCCATCGTCAACTACCACCGCCATCCGATTCGCCGTTTCGAGCTGGTCGT
GGGGATCGGCTATGGCGACAGTATGGAGCAGGCGCGCACCGAATTGCTGGCGCTCGCCGAGGCAGACGAGCGGGTGCTCG
AAGATCCGGCGCCGCAGACCTTCGTCAATTCGCTCGACGACAGTTCGGTCGGCATCGGTCTGCGAGTATGGTGCACCACC
GCAGATTACGTAGCCGTCGGGTGGGATATGACCGAGGCGGTCAAGGCCCGCTTCGACGATGTCGGCATCTCGATCCCGTT
CCCGCAGCGCGAGATTACGCAGCGGGCGGTTTAG

Upstream 100 bases:

>100_bases
GGCGAAAGCATGGACCGGAGGCCGGGGTGGCCAGAGCAGCGCGAATTCCCTATCGCGCCCCGCATATCGACCCCACCTCC
CAAGATTTCAATGGATCGCC

Downstream 100 bases:

>100_bases
CGCCCGGCTTGCGTGCGGGGCGGTGCGCAAGGCCCAGCCGCTCGCGCATCAGCCAGCGCCCGGTCAGCAGCACGGTGGCA
AAGGTCAGGCCGACGGCAAA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MTAEADIAALTIPEQILLWLRLNLIEIAGAFAVLLIGLFLAGLLSRAFERALARSPRFDPTVANFLSNVVKYALWALVLV
TVLAQFGVETTSILAALGGMALAIGLALQGTLSNVASGVMILVQKPFKVGEAINVGSITAVVQNIGLFTTELKQFDGLFV
MIPNSELWNKPIVNYHRHPIRRFELVVGIGYGDSMEQARTELLALAEADERVLEDPAPQTFVNSLDDSSVGIGLRVWCTT
ADYVAVGWDMTEAVKARFDDVGISIPFPQREITQRAV

Sequences:

>Translated_277_residues
MTAEADIAALTIPEQILLWLRLNLIEIAGAFAVLLIGLFLAGLLSRAFERALARSPRFDPTVANFLSNVVKYALWALVLV
TVLAQFGVETTSILAALGGMALAIGLALQGTLSNVASGVMILVQKPFKVGEAINVGSITAVVQNIGLFTTELKQFDGLFV
MIPNSELWNKPIVNYHRHPIRRFELVVGIGYGDSMEQARTELLALAEADERVLEDPAPQTFVNSLDDSSVGIGLRVWCTT
ADYVAVGWDMTEAVKARFDDVGISIPFPQREITQRAV
>Mature_276_residues
TAEADIAALTIPEQILLWLRLNLIEIAGAFAVLLIGLFLAGLLSRAFERALARSPRFDPTVANFLSNVVKYALWALVLVT
VLAQFGVETTSILAALGGMALAIGLALQGTLSNVASGVMILVQKPFKVGEAINVGSITAVVQNIGLFTTELKQFDGLFVM
IPNSELWNKPIVNYHRHPIRRFELVVGIGYGDSMEQARTELLALAEADERVLEDPAPQTFVNSLDDSSVGIGLRVWCTTA
DYVAVGWDMTEAVKARFDDVGISIPFPQREITQRAV

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=253, Percent_Identity=36.7588932806324, Blast_Score=200, Evalue=8e-53,
Organism=Escherichia coli, GI1786670, Length=209, Percent_Identity=24.4019138755981, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787591, Length=224, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI2367355, Length=241, Percent_Identity=21.9917012448133, Blast_Score=71, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 30069; Mature: 29938

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAEADIAALTIPEQILLWLRLNLIEIAGAFAVLLIGLFLAGLLSRAFERALARSPRFDP
CCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
TVANFLSNVVKYALWALVLVTVLAQFGVETTSILAALGGMALAIGLALQGTLSNVASGVM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
ILVQKPFKVGEAINVGSITAVVQNIGLFTTELKQFDGLFVMIPNSELWNKPIVNYHRHPI
EEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHCCCCCHHHCCCHH
RRFELVVGIGYGDSMEQARTELLALAEADERVLEDPAPQTFVNSLDDSSVGIGLRVWCTT
HHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEEEEEC
ADYVAVGWDMTEAVKARFDDVGISIPFPQREITQRAV
CCEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHCCC
>Mature Secondary Structure 
TAEADIAALTIPEQILLWLRLNLIEIAGAFAVLLIGLFLAGLLSRAFERALARSPRFDP
CCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
TVANFLSNVVKYALWALVLVTVLAQFGVETTSILAALGGMALAIGLALQGTLSNVASGVM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
ILVQKPFKVGEAINVGSITAVVQNIGLFTTELKQFDGLFVMIPNSELWNKPIVNYHRHPI
EEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHCCCCCHHHCCCHH
RRFELVVGIGYGDSMEQARTELLALAEADERVLEDPAPQTFVNSLDDSSVGIGLRVWCTT
HHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEEEEEC
ADYVAVGWDMTEAVKARFDDVGISIPFPQREITQRAV
CCEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]