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The map label for this gene is prfC [H]

Identifier: 85375597

GI number: 85375597

Start: 2790193

End: 2791725

Strand: Direct

Name: prfC [H]

Synonym: ELI_13850

Alternate gene names: 85375597

Gene position: 2790193-2791725 (Clockwise)

Preceding gene: 85375596

Following gene: 85375598

Centisome position: 91.41

GC content: 63.73

Gene sequence:

>1533_bases
ATGTCGAACAGGCGGACCTTCGCGATCATCTCGCACCCCGATGCGGGTAAGACCACGCTGACGGAAAAGCTGCTGCTGCA
AGGCGGGGCCATCCACCAAGCGGGTCAGGTCAAGGCGCGCGGCGAAGCGCGGCGGGCACGCTCGGACTGGATGAAGATCG
AGCAACAGCGCGGTATCTCGGTCACCTCCAGCGTGATGACTTTCGAGAAGGAGTACGGTGGCGAAACCATCACCTTCAAC
CTGCTCGACACGCCGGGGCACGAGGACTTCTCCGAAGACACCTATCGCACGCTGACCGCGGTCGATTCCGCCATCATGGT
GATCGACGCGGCGAAGGGGATCGAGCCGCAGACGCTGAAACTGTTCGAAGTGTGCCGCCTGCGCAGCGTGCCGATCATCA
CCTTCGTCAACAAGGTCGATCGCGAAGGCCGCTCGGGCTTCGAACTGCTCGACGAAGTGGCTGACCAGCTCGCGCTCGAT
GTGTCCCCGCAAAGTTGGCCCATCGGCATGGGCGGGCAATTCGAAGGCATCCTCGATTTCGCCAGCGGCACCGTCGCACG
GCCCGAAGGCGGGTCGAAGGAATTCCTCGGCACGCGCGACGAGAACCCGGACATCCCCGAGCAATGGGCGGAGGAGGCCG
AGCTGGCGCAGGTCGGCTATCCCGAATTCGATCTGGAGGCCTATCGCAACGGCGACCTGACGCCGGTCTTCTTCGGTTCG
GCGCTCAAGAGCTTCGGCGTCACCGAGCTGATCGACGCCATCGCCGCCCATGCCCCGCCGCCACGCCCGCAGCCGAGCGA
AGACGGTGCCATCGCGCCCGAGCGCGACGAGGTCACCGGCTTCATCTTCAAGGTGCAGGCCAATATGGACCCGAACCACC
GCGACCGCATCGCATTCATGCGGCAGGTCTCGGGCACCTTCAAACGCGGCATGAAGCTGACGCCCTCGGGCCTTGGCAAG
CCGATTGCGATCCACTCGCCGATCCTCTTTTTCGCGCAGGATCGCGAGATTGCCGACACCGCCGAGGCGGGCGACATCAT
CGGCATTCCCAATCACGGCACGCTCCGCGTCGGCGATACGCTGAGTGAGAAGAACGATGTCCGCTTCACCGGCCTGCCCA
ATTTCGCGCCGGAAATCCTGCGCCGGGTCCAGCTCAAGGATCCGACCAAGACCAAGCAATTGCGCAAGGCGCTCGACGAC
CTGTCAGAAGAGGGCGTGATCCAGGTCTTCTACCCGGAGATCGGCGCGCAGCACATTGTCGGCGTCGTCGGACAGCTGCA
GCTCGAAGTGCTGATCTCGCGGCTGTCGGCAGAGTACAAGGTCGAGGCCGCGCTCGAAGCCGCACCCTTCGCCACCGCAC
GCTGGATCAAGGGCGATGCCAAGGCGATGAGCGAGTTCGAAAGCTTCAACCGCGCCAACCTCGCCAAGGATCGCGACGGG
GACCTGGTGTTCATGGCCAAGAGCCCGTGGGACGTGAACTATCAGCAGGAAAAGAACCCCGACCTGGCGTTTTCCGCGAC
GAAGGAACGATAA

Upstream 100 bases:

>100_bases
CAAGGACGAAGAGATCAAGGCGATCTCCGACAAAGTCGTTGCGGCTGCCGCCAAGCTGGGAGCGGAATTGCGCGGCTAAG
CCGGCGCATACCGAACATTC

Downstream 100 bases:

>100_bases
TTCTCGCCTCCCGCTTGCGGGAGGGGTCGGGGTGGGCGCGGAGCCATCGCCGCCGGGATTGCCCAATCGGCTGCGACTAG
GCAGACTGGCTGCCTAGTCT

Product: peptide chain release factor

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MSNRRTFAIISHPDAGKTTLTEKLLLQGGAIHQAGQVKARGEARRARSDWMKIEQQRGISVTSSVMTFEKEYGGETITFN
LLDTPGHEDFSEDTYRTLTAVDSAIMVIDAAKGIEPQTLKLFEVCRLRSVPIITFVNKVDREGRSGFELLDEVADQLALD
VSPQSWPIGMGGQFEGILDFASGTVARPEGGSKEFLGTRDENPDIPEQWAEEAELAQVGYPEFDLEAYRNGDLTPVFFGS
ALKSFGVTELIDAIAAHAPPPRPQPSEDGAIAPERDEVTGFIFKVQANMDPNHRDRIAFMRQVSGTFKRGMKLTPSGLGK
PIAIHSPILFFAQDREIADTAEAGDIIGIPNHGTLRVGDTLSEKNDVRFTGLPNFAPEILRRVQLKDPTKTKQLRKALDD
LSEEGVIQVFYPEIGAQHIVGVVGQLQLEVLISRLSAEYKVEAALEAAPFATARWIKGDAKAMSEFESFNRANLAKDRDG
DLVFMAKSPWDVNYQQEKNPDLAFSATKER

Sequences:

>Translated_510_residues
MSNRRTFAIISHPDAGKTTLTEKLLLQGGAIHQAGQVKARGEARRARSDWMKIEQQRGISVTSSVMTFEKEYGGETITFN
LLDTPGHEDFSEDTYRTLTAVDSAIMVIDAAKGIEPQTLKLFEVCRLRSVPIITFVNKVDREGRSGFELLDEVADQLALD
VSPQSWPIGMGGQFEGILDFASGTVARPEGGSKEFLGTRDENPDIPEQWAEEAELAQVGYPEFDLEAYRNGDLTPVFFGS
ALKSFGVTELIDAIAAHAPPPRPQPSEDGAIAPERDEVTGFIFKVQANMDPNHRDRIAFMRQVSGTFKRGMKLTPSGLGK
PIAIHSPILFFAQDREIADTAEAGDIIGIPNHGTLRVGDTLSEKNDVRFTGLPNFAPEILRRVQLKDPTKTKQLRKALDD
LSEEGVIQVFYPEIGAQHIVGVVGQLQLEVLISRLSAEYKVEAALEAAPFATARWIKGDAKAMSEFESFNRANLAKDRDG
DLVFMAKSPWDVNYQQEKNPDLAFSATKER
>Mature_509_residues
SNRRTFAIISHPDAGKTTLTEKLLLQGGAIHQAGQVKARGEARRARSDWMKIEQQRGISVTSSVMTFEKEYGGETITFNL
LDTPGHEDFSEDTYRTLTAVDSAIMVIDAAKGIEPQTLKLFEVCRLRSVPIITFVNKVDREGRSGFELLDEVADQLALDV
SPQSWPIGMGGQFEGILDFASGTVARPEGGSKEFLGTRDENPDIPEQWAEEAELAQVGYPEFDLEAYRNGDLTPVFFGSA
LKSFGVTELIDAIAAHAPPPRPQPSEDGAIAPERDEVTGFIFKVQANMDPNHRDRIAFMRQVSGTFKRGMKLTPSGLGKP
IAIHSPILFFAQDREIADTAEAGDIIGIPNHGTLRVGDTLSEKNDVRFTGLPNFAPEILRRVQLKDPTKTKQLRKALDDL
SEEGVIQVFYPEIGAQHIVGVVGQLQLEVLISRLSAEYKVEAALEAAPFATARWIKGDAKAMSEFESFNRANLAKDRDGD
LVFMAKSPWDVNYQQEKNPDLAFSATKER

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides but is not codon-specific. It may interact directly with the ribosome. The stimulation of RF-1 and RF-2 is significantly re

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=483, Percent_Identity=26.0869565217391, Blast_Score=143, Evalue=5e-34,
Organism=Homo sapiens, GI19923640, Length=524, Percent_Identity=26.3358778625954, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI25306283, Length=395, Percent_Identity=28.1012658227848, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI25306287, Length=292, Percent_Identity=30.1369863013699, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI157426893, Length=140, Percent_Identity=32.8571428571429, Blast_Score=90, Evalue=6e-18,
Organism=Homo sapiens, GI94966754, Length=140, Percent_Identity=32.8571428571429, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI310132016, Length=118, Percent_Identity=36.4406779661017, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI310110807, Length=118, Percent_Identity=36.4406779661017, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI310123363, Length=118, Percent_Identity=36.4406779661017, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI4503483, Length=152, Percent_Identity=30.9210526315789, Blast_Score=76, Evalue=7e-14,
Organism=Escherichia coli, GI1790835, Length=524, Percent_Identity=48.8549618320611, Blast_Score=498, Evalue=1e-142,
Organism=Escherichia coli, GI1789738, Length=503, Percent_Identity=27.2365805168986, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1788922, Length=163, Percent_Identity=33.1288343558282, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI48994988, Length=177, Percent_Identity=33.3333333333333, Blast_Score=86, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17533571, Length=489, Percent_Identity=27.8118609406953, Blast_Score=148, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI17556745, Length=477, Percent_Identity=25.7861635220126, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17557151, Length=139, Percent_Identity=32.3741007194245, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17506493, Length=165, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=477, Percent_Identity=23.8993710691824, Blast_Score=147, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6322359, Length=519, Percent_Identity=25.0481695568401, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6324707, Length=153, Percent_Identity=33.9869281045752, Blast_Score=83, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=175, Percent_Identity=28, Blast_Score=83, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6320593, Length=153, Percent_Identity=33.9869281045752, Blast_Score=83, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24582462, Length=484, Percent_Identity=26.8595041322314, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI221458488, Length=510, Percent_Identity=25.6862745098039, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI78706572, Length=136, Percent_Identity=34.5588235294118, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24585711, Length=157, Percent_Identity=34.3949044585987, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24585713, Length=157, Percent_Identity=34.3949044585987, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24585709, Length=157, Percent_Identity=34.3949044585987, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24656849, Length=143, Percent_Identity=29.3706293706294, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI28574573, Length=144, Percent_Identity=34.0277777777778, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 56252; Mature: 56121

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNRRTFAIISHPDAGKTTLTEKLLLQGGAIHQAGQVKARGEARRARSDWMKIEQQRGIS
CCCCEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
VTSSVMTFEKEYGGETITFNLLDTPGHEDFSEDTYRTLTAVDSAIMVIDAAKGIEPQTLK
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCCHHHH
LFEVCRLRSVPIITFVNKVDREGRSGFELLDEVADQLALDVSPQSWPIGMGGQFEGILDF
HHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHEECCCCCCCCCCCCCCCHHHHHH
ASGTVARPEGGSKEFLGTRDENPDIPEQWAEEAELAQVGYPEFDLEAYRNGDLTPVFFGS
CCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHHH
ALKSFGVTELIDAIAAHAPPPRPQPSEDGAIAPERDEVTGFIFKVQANMDPNHRDRIAFM
HHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHH
RQVSGTFKRGMKLTPSGLGKPIAIHSPILFFAQDREIADTAEAGDIIGIPNHGTLRVGDT
HHHHHHHHCCCCCCCCCCCCCEEECCCEEEEECCCCHHHHCCCCCEEECCCCCEEEECCC
LSEKNDVRFTGLPNFAPEILRRVQLKDPTKTKQLRKALDDLSEEGVIQVFYPEIGAQHIV
CCCCCCEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHH
GVVGQLQLEVLISRLSAEYKVEAALEAAPFATARWIKGDAKAMSEFESFNRANLAKDRDG
HHHHHHHHHHHHHHHCCCCEEHHHHHHCCCHHHHHHCCHHHHHHHHHHHCHHCCCCCCCC
DLVFMAKSPWDVNYQQEKNPDLAFSATKER
CEEEEECCCCCCCCCCCCCCCEEEECCCCC
>Mature Secondary Structure 
SNRRTFAIISHPDAGKTTLTEKLLLQGGAIHQAGQVKARGEARRARSDWMKIEQQRGIS
CCCEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
VTSSVMTFEKEYGGETITFNLLDTPGHEDFSEDTYRTLTAVDSAIMVIDAAKGIEPQTLK
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCCHHHH
LFEVCRLRSVPIITFVNKVDREGRSGFELLDEVADQLALDVSPQSWPIGMGGQFEGILDF
HHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHEECCCCCCCCCCCCCCCHHHHHH
ASGTVARPEGGSKEFLGTRDENPDIPEQWAEEAELAQVGYPEFDLEAYRNGDLTPVFFGS
CCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHHH
ALKSFGVTELIDAIAAHAPPPRPQPSEDGAIAPERDEVTGFIFKVQANMDPNHRDRIAFM
HHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHH
RQVSGTFKRGMKLTPSGLGKPIAIHSPILFFAQDREIADTAEAGDIIGIPNHGTLRVGDT
HHHHHHHHCCCCCCCCCCCCCEEECCCEEEEECCCCHHHHCCCCCEEECCCCCEEEECCC
LSEKNDVRFTGLPNFAPEILRRVQLKDPTKTKQLRKALDDLSEEGVIQVFYPEIGAQHIV
CCCCCCEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHH
GVVGQLQLEVLISRLSAEYKVEAALEAAPFATARWIKGDAKAMSEFESFNRANLAKDRDG
HHHHHHHHHHHHHHHCCCCEEHHHHHHCCCHHHHHHCCHHHHHHHHHHHCHHCCCCCCCC
DLVFMAKSPWDVNYQQEKNPDLAFSATKER
CEEEEECCCCCCCCCCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7539688 [H]