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The map label for this gene is pheS

Identifier: 85375595

GI number: 85375595

Start: 2786642

End: 2787742

Strand: Direct

Name: pheS

Synonym: ELI_13840

Alternate gene names: 85375595

Gene position: 2786642-2787742 (Clockwise)

Preceding gene: 85375594

Following gene: 85375596

Centisome position: 91.29

GC content: 65.12

Gene sequence:

>1101_bases
ATGACAGATTTGACCAACCAGAAAGATGCGGCATTGGCCGCGATCGCCGCTGCGACGAGCCTCGATGCGCTCGAAGAGCA
GCGCGTGGCTGCGCTCGGCAAGAAAGGCTGGGTCAGCCTCGCCCTCAAGACGCTCGGCGGGATGGACCCGGAAGAGCGCC
AGAAGGCAGCGCCCGCGATCCAGGCCGTTCGCGCCGAAGTTGCCGATGCGATCGCCGCGCGCAAGGACGCGCTGGAGCGC
GAGGCGCTGGAAGCCCAGCTCGCAACCGAAACGCTCGATCTTACTTTGCCCGCGCCGCAGCAGCGCTTCGGCTCGGTCCA
CCCGGTCAGCCAGGTGATGGACGAACTGGCGGAAATCTTCGCCGACCTCGGTTTCGCTGTCGCCACGGGGCCAGAGATCG
AAGACGACTGGCACAATTTCACCGCGCTCAACATGGCCGAAACGCATCCGGCGCGGGCGATGCACGATACGTTCTATTTC
CCCGATGTGGATAGCGAAGGGCGGCGCATGCTGCTGCGTACACATACCTCGCCGGTGCAGATCCGCGCGATGAAGGCGCA
AGGCGCGCCGATCCGCATCATCGCGCCGGGCCGCGTCTATCGCAGCGACAGCGACGCCACCCACACGCCGATGTTCCACC
AGGTCGAAGGTCTCGTAATCGACCGCGACATCCACCTGGGCCATCTCAAGTGGACGCTGGAGACTTTCCTCAAGGCCTTC
TTCGAGCGCGAGGATATCGTCCTGCGCCTGCGCCCGTCCTATTTCCCTTTCACCGAGCCTTCGGTCGAAGTCGATGTCGG
CTATTCGCGCGAGGGCGGTCGCCGCGTTGTGGGCGGGGACGGCAATGCCGAAGGGCATGACTGGATGGAGCTGCTTGGCT
CCGGCATGGTCAACCGCCGCGTGATCGAAATGGCGGGGCTCGACCCCGACGAATGGCAGGGTTTCGCCTGGGGCCTCGGC
GTCGACCGGCTCGCCATGCTCAAATACGGCATGGACGATCTGCGCGCCTTCTTCGACGGCGACCAGCGCTGGCTCGACCA
TTACGGGTTCAGCCCGTTCGACCAGCCGACCCTGTCTGCAGGTGTGGGAGCGCGGTCATGA

Upstream 100 bases:

>100_bases
CCCGGCCCGCGCCCCGCGGACATGCGCGCGGCAAACACAAGCACTTTGCCTTGCGCGCCATTTGCCGCTAGCGGGGCGCC
CTTCGATAAAGACACAGCAC

Downstream 100 bases:

>100_bases
AGTTCTCCATCACCTGGCTCAGGGATCATCTCGAAACCGAGGCGGGTCTCGAAGAGATCGTCGCCACGCTCAACCGGATC
GGCCACGAGGTCGAAGGGGT

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MTDLTNQKDAALAAIAAATSLDALEEQRVAALGKKGWVSLALKTLGGMDPEERQKAAPAIQAVRAEVADAIAARKDALER
EALEAQLATETLDLTLPAPQQRFGSVHPVSQVMDELAEIFADLGFAVATGPEIEDDWHNFTALNMAETHPARAMHDTFYF
PDVDSEGRRMLLRTHTSPVQIRAMKAQGAPIRIIAPGRVYRSDSDATHTPMFHQVEGLVIDRDIHLGHLKWTLETFLKAF
FEREDIVLRLRPSYFPFTEPSVEVDVGYSREGGRRVVGGDGNAEGHDWMELLGSGMVNRRVIEMAGLDPDEWQGFAWGLG
VDRLAMLKYGMDDLRAFFDGDQRWLDHYGFSPFDQPTLSAGVGARS

Sequences:

>Translated_366_residues
MTDLTNQKDAALAAIAAATSLDALEEQRVAALGKKGWVSLALKTLGGMDPEERQKAAPAIQAVRAEVADAIAARKDALER
EALEAQLATETLDLTLPAPQQRFGSVHPVSQVMDELAEIFADLGFAVATGPEIEDDWHNFTALNMAETHPARAMHDTFYF
PDVDSEGRRMLLRTHTSPVQIRAMKAQGAPIRIIAPGRVYRSDSDATHTPMFHQVEGLVIDRDIHLGHLKWTLETFLKAF
FEREDIVLRLRPSYFPFTEPSVEVDVGYSREGGRRVVGGDGNAEGHDWMELLGSGMVNRRVIEMAGLDPDEWQGFAWGLG
VDRLAMLKYGMDDLRAFFDGDQRWLDHYGFSPFDQPTLSAGVGARS
>Mature_365_residues
TDLTNQKDAALAAIAAATSLDALEEQRVAALGKKGWVSLALKTLGGMDPEERQKAAPAIQAVRAEVADAIAARKDALERE
ALEAQLATETLDLTLPAPQQRFGSVHPVSQVMDELAEIFADLGFAVATGPEIEDDWHNFTALNMAETHPARAMHDTFYFP
DVDSEGRRMLLRTHTSPVQIRAMKAQGAPIRIIAPGRVYRSDSDATHTPMFHQVEGLVIDRDIHLGHLKWTLETFLKAFF
EREDIVLRLRPSYFPFTEPSVEVDVGYSREGGRRVVGGDGNAEGHDWMELLGSGMVNRRVIEMAGLDPDEWQGFAWGLGV
DRLAMLKYGMDDLRAFFDGDQRWLDHYGFSPFDQPTLSAGVGARS

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=273, Percent_Identity=32.6007326007326, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI5729820, Length=290, Percent_Identity=27.5862068965517, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1788007, Length=348, Percent_Identity=50.8620689655172, Blast_Score=342, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI32563657, Length=275, Percent_Identity=30.9090909090909, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17508957, Length=275, Percent_Identity=30.9090909090909, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI32566635, Length=260, Percent_Identity=30.3846153846154, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321087, Length=392, Percent_Identity=26.530612244898, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6325304, Length=328, Percent_Identity=23.4756097560976, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI18858079, Length=282, Percent_Identity=31.9148936170213, Blast_Score=112, Evalue=3e-25,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_ERYLH (Q2N631)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_459657.1
- ProteinModelPortal:   Q2N631
- SMR:   Q2N631
- STRING:   Q2N631
- GeneID:   3870468
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_13840
- NMPDR:   fig|314225.3.peg.250
- eggNOG:   COG0016
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- PhylomeDB:   Q2N631
- ProtClustDB:   PRK00488
- BioCyc:   ELIT314225:ELI_13840-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 40473; Mature: 40342

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLTNQKDAALAAIAAATSLDALEEQRVAALGKKGWVSLALKTLGGMDPEERQKAAPAI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHH
QAVRAEVADAIAARKDALEREALEAQLATETLDLTLPAPQQRFGSVHPVSQVMDELAEIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHCCCCCHHHHHHHHHHHHH
ADLGFAVATGPEIEDDWHNFTALNMAETHPARAMHDTFYFPDVDSEGRRMLLRTHTSPVQ
HHCCCEEECCCCCCCCCCCEEEEECHHCCCCHHHHCCCCCCCCCCCCCEEEEEECCCCEE
IRAMKAQGAPIRIIAPGRVYRSDSDATHTPMFHQVEGLVIDRDIHLGHLKWTLETFLKAF
EEEEECCCCCEEEEECCEEECCCCCCCCCCHHHHHCCEEEECCEEEEHHHHHHHHHHHHH
FEREDIVLRLRPSYFPFTEPSVEVDVGYSREGGRRVVGGDGNAEGHDWMELLGSGMVNRR
HCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCEEEECCCCCCCHHHHHHHHHCCCHHHH
VIEMAGLDPDEWQGFAWGLGVDRLAMLKYGMDDLRAFFDGDQRWLDHYGFSPFDQPTLSA
HHHHCCCCHHHCCCEEECCCHHHHHHHHHCHHHHHHHHCCHHHHHHHCCCCCCCCCCHHC
GVGARS
CCCCCC
>Mature Secondary Structure 
TDLTNQKDAALAAIAAATSLDALEEQRVAALGKKGWVSLALKTLGGMDPEERQKAAPAI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHH
QAVRAEVADAIAARKDALEREALEAQLATETLDLTLPAPQQRFGSVHPVSQVMDELAEIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHCCCCCHHHHHHHHHHHHH
ADLGFAVATGPEIEDDWHNFTALNMAETHPARAMHDTFYFPDVDSEGRRMLLRTHTSPVQ
HHCCCEEECCCCCCCCCCCEEEEECHHCCCCHHHHCCCCCCCCCCCCCEEEEEECCCCEE
IRAMKAQGAPIRIIAPGRVYRSDSDATHTPMFHQVEGLVIDRDIHLGHLKWTLETFLKAF
EEEEECCCCCEEEEECCEEECCCCCCCCCCHHHHHCCEEEECCEEEEHHHHHHHHHHHHH
FEREDIVLRLRPSYFPFTEPSVEVDVGYSREGGRRVVGGDGNAEGHDWMELLGSGMVNRR
HCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCEEEECCCCCCCHHHHHHHHHCCCHHHH
VIEMAGLDPDEWQGFAWGLGVDRLAMLKYGMDDLRAFFDGDQRWLDHYGFSPFDQPTLSA
HHHHCCCCHHHCCCEEECCCHHHHHHHHHCHHHHHHHHCCHHHHHHHCCCCCCCCCCHHC
GVGARS
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA