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The map label for this gene is mycA [H]

Identifier: 85375056

GI number: 85375056

Start: 2248852

End: 2250585

Strand: Direct

Name: mycA [H]

Synonym: ELI_11145

Alternate gene names: 85375056

Gene position: 2248852-2250585 (Clockwise)

Preceding gene: 85375051

Following gene: 85375058

Centisome position: 73.67

GC content: 63.38

Gene sequence:

>1734_bases
ATGACTGATCAGACGCTGACACCGACACCCATCGACTGCCCGCTGCCGCGCCGTCGGTCGGATTTCGACACCTTTGCCGA
AGCCATCGACTACGCCGCGCGCAGCGAGAAGGGCATGAACTTCCACGATATGCGTGGCCAGTTGGAGCGCGTATATCCCT
ATCGCCAGATGCGCGAAGACGCCCTGCAGATGGCCCGTCGCCTGGTCGCCGCCGGGGTCGAAAAGGAAGACCGGGTCGCG
CTGATCGCCGAGACCGTGCCGGAGTTCACCGCGATGTTCTGCGCTTGCGTCTACGTCGGTGCCTGGCCGGTGCCCCTGCC
CCTGCCCACGACCTTCGGCGGCAAGGGCAGCTATGTCGAACAGCTGGGCGTTCAGCTTTCAAGCAGCGACCCCGCCTTTC
TGTTTTACCCGCCCGAAATAGCGGAAATGGCCGAAGAAGCAGTCGCCAAGCAGGGCTGCAAGGGTGCGGACTGGACCAGC
TTTGCCGAACGCGATGCGCCGGATTGCGAATTTCCGGAGATCACGCCCGACGATATTTGTTACCTGCAATATTCGAGCGG
CTCGACCCGCTTCCCGACCGGCGTCGCCGTCACGCACCGCGCGCTGCTGCATAACCTCTCGGGCCACGCCATCACCATGA
ATATCGGCGAAGGCGACCGCGGGGTAAGCTGGCTGCCGTGGTATCACGACATGGGCCTGGTCGGTTGTTTCCTGTCGATG
GTCGGCAACCAGGTCAGCTCGGATTATCTCAAGACCGAGCACTTCGCCCGCCGCCCGCTGGCATGGCTCGACATGATTAC
CCGCAATCCCGGGCAGACCATGAGCTATTCGCCCACCTTCGGCTACGACATCTGCGCTCGCCGCATCTCCAGCCAAAGCC
ATGTCGACGAACGTTTCGACCTCTCGCGCTGGCGGCTCGCGGGCAATGGCGCGGACATGATCCGGCCCGACGTGATGCAA
AGCTTCGTCAACGCTTTCGCGCCTGCCGGATTCAAGGCCAGCGCGTTCACGCCCAGCTACGGCCTTGCCGAAGCGACGCT
GGCAGTGACGGTCATGCCGCCGGGCGAAGGCATCAGGGTCGAGCTGGTCGAGGAAGAGCGGCTGTCGGGCCAGCATCGCG
ATCTCAGCAAACCGGCGCGGTATCGTGCGATCGTCAATTGCGGCAAGCCACTGCCCGACATGGACGTCGAGATCCGCGGG
CCCGGTGACGAGGTCAAGGGCGACCACCAGATCGGCAAAGTGTGGTGCCGCGGGCCGAGCGTCATGCATTCCTATTTCCG
CGATCCCGAAGCTACCGACGATTGCCTGGTGGATGGTTGGCTCGACACCGGCGACATGGGCTACATGGCCGATGGCTACC
TGTTCATCGTCGGTCGCGCCAAGGACATGATCATCATCAACGGCAAGAACCACTGGCCGCAGGATATCGAATGGGCCGTC
GAACAGCTCCCCGGCTTCAATCATGGGGACATCGCCGCATTCTCGCTCGAGACCGAGAATGGCGAGGAAGCCCCCGCCGT
ACTGGTCCATTGCCGCGTGTCCGATCCGCAGGAACGGGCCAAGCTGCACAGTGTCATCAAGGAAAAAGTGCAGGCCGTCA
CCGGAACAAGCTGCGTCGTCGAACTGGTGCCGCCGCGAACCCTGCCGCGCACGAGCTCAGGCAAGCTGAGCCGCGCCAAG
GCCAAGAAGCTCTACCTCTCGGGTGAAATCCAGCCGCTGGATCTGGCCGCCTGA

Upstream 100 bases:

>100_bases
AATGGGAAAATAATATGACGGTGCAAAGACATCAAATTGCCCCGCGCTCAGGGACAGGATGCGCCTAAGTGCGCGTAATC
ACACGGGTTTCGCATAAAAT

Downstream 100 bases:

>100_bases
CCGGCGAGGGAGCCTACTGCGCGATCACCCATTTGCCTTGGCGGAAGCGATAGATATGCGGGCTGACGCAGCGCGGCTGC
GCCGCGCAGTCTTCCTCGAC

Product: acyl-CoA synthetase

Products: AMP; diphosphate; acyl-CoA

Alternate protein names: Glutamate-1-semialdehyde aminotransferase; GSA-AT; ATP-dependent asparagine adenylase 1; AsnA 1; Asparagine activase 1 [H]

Number of amino acids: Translated: 577; Mature: 576

Protein sequence:

>577_residues
MTDQTLTPTPIDCPLPRRRSDFDTFAEAIDYAARSEKGMNFHDMRGQLERVYPYRQMREDALQMARRLVAAGVEKEDRVA
LIAETVPEFTAMFCACVYVGAWPVPLPLPTTFGGKGSYVEQLGVQLSSSDPAFLFYPPEIAEMAEEAVAKQGCKGADWTS
FAERDAPDCEFPEITPDDICYLQYSSGSTRFPTGVAVTHRALLHNLSGHAITMNIGEGDRGVSWLPWYHDMGLVGCFLSM
VGNQVSSDYLKTEHFARRPLAWLDMITRNPGQTMSYSPTFGYDICARRISSQSHVDERFDLSRWRLAGNGADMIRPDVMQ
SFVNAFAPAGFKASAFTPSYGLAEATLAVTVMPPGEGIRVELVEEERLSGQHRDLSKPARYRAIVNCGKPLPDMDVEIRG
PGDEVKGDHQIGKVWCRGPSVMHSYFRDPEATDDCLVDGWLDTGDMGYMADGYLFIVGRAKDMIIINGKNHWPQDIEWAV
EQLPGFNHGDIAAFSLETENGEEAPAVLVHCRVSDPQERAKLHSVIKEKVQAVTGTSCVVELVPPRTLPRTSSGKLSRAK
AKKLYLSGEIQPLDLAA

Sequences:

>Translated_577_residues
MTDQTLTPTPIDCPLPRRRSDFDTFAEAIDYAARSEKGMNFHDMRGQLERVYPYRQMREDALQMARRLVAAGVEKEDRVA
LIAETVPEFTAMFCACVYVGAWPVPLPLPTTFGGKGSYVEQLGVQLSSSDPAFLFYPPEIAEMAEEAVAKQGCKGADWTS
FAERDAPDCEFPEITPDDICYLQYSSGSTRFPTGVAVTHRALLHNLSGHAITMNIGEGDRGVSWLPWYHDMGLVGCFLSM
VGNQVSSDYLKTEHFARRPLAWLDMITRNPGQTMSYSPTFGYDICARRISSQSHVDERFDLSRWRLAGNGADMIRPDVMQ
SFVNAFAPAGFKASAFTPSYGLAEATLAVTVMPPGEGIRVELVEEERLSGQHRDLSKPARYRAIVNCGKPLPDMDVEIRG
PGDEVKGDHQIGKVWCRGPSVMHSYFRDPEATDDCLVDGWLDTGDMGYMADGYLFIVGRAKDMIIINGKNHWPQDIEWAV
EQLPGFNHGDIAAFSLETENGEEAPAVLVHCRVSDPQERAKLHSVIKEKVQAVTGTSCVVELVPPRTLPRTSSGKLSRAK
AKKLYLSGEIQPLDLAA
>Mature_576_residues
TDQTLTPTPIDCPLPRRRSDFDTFAEAIDYAARSEKGMNFHDMRGQLERVYPYRQMREDALQMARRLVAAGVEKEDRVAL
IAETVPEFTAMFCACVYVGAWPVPLPLPTTFGGKGSYVEQLGVQLSSSDPAFLFYPPEIAEMAEEAVAKQGCKGADWTSF
AERDAPDCEFPEITPDDICYLQYSSGSTRFPTGVAVTHRALLHNLSGHAITMNIGEGDRGVSWLPWYHDMGLVGCFLSMV
GNQVSSDYLKTEHFARRPLAWLDMITRNPGQTMSYSPTFGYDICARRISSQSHVDERFDLSRWRLAGNGADMIRPDVMQS
FVNAFAPAGFKASAFTPSYGLAEATLAVTVMPPGEGIRVELVEEERLSGQHRDLSKPARYRAIVNCGKPLPDMDVEIRGP
GDEVKGDHQIGKVWCRGPSVMHSYFRDPEATDDCLVDGWLDTGDMGYMADGYLFIVGRAKDMIIINGKNHWPQDIEWAVE
QLPGFNHGDIAAFSLETENGEEAPAVLVHCRVSDPQERAKLHSVIKEKVQAVTGTSCVVELVPPRTLPRTSSGKLSRAKA
KKLYLSGEIQPLDLAA

Specific function: This protein is a multifunctional enzyme, able to activate a long chain fatty acid and link it with the amino acid Asn as part of the synthesis of mycosubtilin. The activation sites consist of individual domains [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 acyl carrier domains [H]

Homologues:

Organism=Escherichia coli, GI1788107, Length=609, Percent_Identity=23.8095238095238, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI145693145, Length=544, Percent_Identity=25, Blast_Score=76, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17559526, Length=541, Percent_Identity=23.1053604436229, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17531443, Length=549, Percent_Identity=20.5828779599271, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI32563687, Length=502, Percent_Identity=23.9043824701195, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71996755, Length=526, Percent_Identity=19.9619771863118, Blast_Score=83, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17558820, Length=441, Percent_Identity=21.9954648526077, Blast_Score=80, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17560308, Length=318, Percent_Identity=26.1006289308176, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI20130357, Length=181, Percent_Identity=25.9668508287293, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24648257, Length=397, Percent_Identity=22.1662468513854, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24648260, Length=178, Percent_Identity=29.7752808988764, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21356947, Length=189, Percent_Identity=28.5714285714286, Blast_Score=66, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR005814
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR023213
- InterPro:   IPR001242
- InterPro:   IPR018201
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR006163
- InterPro:   IPR020806
- InterPro:   IPR006162
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF00202 Aminotran_3; PF00501 AMP-binding; PF00668 Condensation; PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C; PF00550 PP-binding [H]

EC number: 6.2.1.3

Molecular weight: Translated: 63889; Mature: 63758

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDQTLTPTPIDCPLPRRRSDFDTFAEAIDYAARSEKGMNFHDMRGQLERVYPYRQMRED
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHH
ALQMARRLVAAGVEKEDRVALIAETVPEFTAMFCACVYVGAWPVPLPLPTTFGGKGSYVE
HHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHH
QLGVQLSSSDPAFLFYPPEIAEMAEEAVAKQGCKGADWTSFAERDAPDCEFPEITPDDIC
HHCCEECCCCCEEEEECHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCCEE
YLQYSSGSTRFPTGVAVTHRALLHNLSGHAITMNIGEGDRGVSWLPWYHDMGLVGCFLSM
EEEECCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCEECCHHHHHHHHHHHHHH
VGNQVSSDYLKTEHFARRPLAWLDMITRNPGQTMSYSPTFGYDICARRISSQSHVDERFD
HCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCEECCCCCCHHHHHHHHCCHHHHHHHHC
LSRWRLAGNGADMIRPDVMQSFVNAFAPAGFKASAFTPSYGLAEATLAVTVMPPGEGIRV
CHHEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCEE
ELVEEERLSGQHRDLSKPARYRAIVNCGKPLPDMDVEIRGPGDEVKGDHQIGKVWCRGPS
EEECHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCEEECCHH
VMHSYFRDPEATDDCLVDGWLDTGDMGYMADGYLFIVGRAKDMIIINGKNHWPQDIEWAV
HHHHHHCCCCCCCCCEEECCCCCCCCCEEECCEEEEEECCCCEEEECCCCCCCHHHHHHH
EQLPGFNHGDIAAFSLETENGEEAPAVLVHCRVSDPQERAKLHSVIKEKVQAVTGTSCVV
HHCCCCCCCCEEEEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEE
ELVPPRTLPRTSSGKLSRAKAKKLYLSGEIQPLDLAA
EECCCCCCCCCCCCCCHHHHHHEEEECCCCCCCCCCC
>Mature Secondary Structure 
TDQTLTPTPIDCPLPRRRSDFDTFAEAIDYAARSEKGMNFHDMRGQLERVYPYRQMRED
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHH
ALQMARRLVAAGVEKEDRVALIAETVPEFTAMFCACVYVGAWPVPLPLPTTFGGKGSYVE
HHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHH
QLGVQLSSSDPAFLFYPPEIAEMAEEAVAKQGCKGADWTSFAERDAPDCEFPEITPDDIC
HHCCEECCCCCEEEEECHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCCEE
YLQYSSGSTRFPTGVAVTHRALLHNLSGHAITMNIGEGDRGVSWLPWYHDMGLVGCFLSM
EEEECCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCEECCHHHHHHHHHHHHHH
VGNQVSSDYLKTEHFARRPLAWLDMITRNPGQTMSYSPTFGYDICARRISSQSHVDERFD
HCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCEECCCCCCHHHHHHHHCCHHHHHHHHC
LSRWRLAGNGADMIRPDVMQSFVNAFAPAGFKASAFTPSYGLAEATLAVTVMPPGEGIRV
CHHEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCEE
ELVEEERLSGQHRDLSKPARYRAIVNCGKPLPDMDVEIRGPGDEVKGDHQIGKVWCRGPS
EEECHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCEEECCHH
VMHSYFRDPEATDDCLVDGWLDTGDMGYMADGYLFIVGRAKDMIIINGKNHWPQDIEWAV
HHHHHHCCCCCCCCCEEECCCCCCCCCEEECCEEEEEECCCCEEEECCCCCCCHHHHHHH
EQLPGFNHGDIAAFSLETENGEEAPAVLVHCRVSDPQERAKLHSVIKEKVQAVTGTSCVV
HHCCCCCCCCEEEEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEE
ELVPPRTLPRTSSGKLSRAKAKKLYLSGEIQPLDLAA
EECCCCCCCCCCCCCCHHHHHHEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; long-chain carboxylic acid; CoA

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10557314 [H]