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The map label for this gene is mutM

Identifier: 85374938

GI number: 85374938

Start: 2140494

End: 2141309

Strand: Direct

Name: mutM

Synonym: ELI_10555

Alternate gene names: 85374938

Gene position: 2140494-2141309 (Clockwise)

Preceding gene: 85374935

Following gene: 85374939

Centisome position: 70.12

GC content: 64.46

Gene sequence:

>816_bases
ATGCCTGAGTTACCTGAAGTCGAAACAACGGTTCGCGGGCTCGCGCGGTTCCTCCAAGGCGAGCGGATCACCCGCACCGT
CACCAACAGGCCCGACATGCGTTTTCCCTTCCCCGATGGTCTGGGGCAGGCGCTCACCGGTGCGACCGTCGTTTCGCTCG
GGCGGCGAGCCAAATACGGGCTGATCCATACCGACAGAGACCAGACGATGATCTTCCATCTCGGGATGAGCGGGCGGTGG
AGGATCGATCCGGACGAAACCGACAAGCACGATCACCTGCTGATCGAAACGGCGGATCATCGCTTCGCGCTCTGCGATCC
GCGCCGTTTCGGCTGGGTCGACCTGGTCGGTACACAGGCGCTCGATCAATGGCCGGGCTTCGCCGCAATGGGGCCGGAGC
CGCTCGGCGATGCGCTGACAATCGAGCATCTCCGTGCCGCGCTCTCCGGACGCAAGCAGGCGATCAAGCTTTGCCTGCTC
GACCAAGCGATCGTGGCGGGCCTCGGCAATATCTATGTCTGCGAAGCGCTCTGGCACGCGCGCATTCATCCTCGCAAGGC
CGGCGGGCGAGTCTCGAAACAGGCGCTTTCTCTCTTGATCACCGCGATCCGCGACGTGCTCGAACAATCGATCCGCGACG
GTGGCTCGAGCCTGCGCGACTATGCCCAGCCCGATGGCGAACTCGGCTATTTCGCTACCCGCTTCCAAGTCTATGGCCGC
GACGGCCAGCCCTGCCACCGCGACGACGGCGGCACGATCCGCCGCTTCGCTCAAGGCGGCCGCAGCACGTGGTATTGTCC
GCGCTGCCAACGTTAG

Upstream 100 bases:

>100_bases
CGGGGGCTACCTCTTCATAGCCGAAGGATACGGTATCGGTCATCGCGTGCGGCCTTAGAGGCAATTGCTACCCCGGCAAA
GGGTGTTAGGAGGTTCGGCA

Downstream 100 bases:

>100_bases
CCTTCCGCACCAACATCCATGGGAGCGTCCTCGCTAGACGTGCAGCAAGCTGCGGCCGCTGCGGGCGAGCGCCTCGGTCA
AGCTACCGCCTGACGGCTAC

Product: formamidopyrimidine-DNA glycosylase

Products: NA

Alternate protein names: Fapy-DNA glycosylase; DNA-(apurinic or apyrimidinic site) lyase mutM; AP lyase mutM

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MPELPEVETTVRGLARFLQGERITRTVTNRPDMRFPFPDGLGQALTGATVVSLGRRAKYGLIHTDRDQTMIFHLGMSGRW
RIDPDETDKHDHLLIETADHRFALCDPRRFGWVDLVGTQALDQWPGFAAMGPEPLGDALTIEHLRAALSGRKQAIKLCLL
DQAIVAGLGNIYVCEALWHARIHPRKAGGRVSKQALSLLITAIRDVLEQSIRDGGSSLRDYAQPDGELGYFATRFQVYGR
DGQPCHRDDGGTIRRFAQGGRSTWYCPRCQR

Sequences:

>Translated_271_residues
MPELPEVETTVRGLARFLQGERITRTVTNRPDMRFPFPDGLGQALTGATVVSLGRRAKYGLIHTDRDQTMIFHLGMSGRW
RIDPDETDKHDHLLIETADHRFALCDPRRFGWVDLVGTQALDQWPGFAAMGPEPLGDALTIEHLRAALSGRKQAIKLCLL
DQAIVAGLGNIYVCEALWHARIHPRKAGGRVSKQALSLLITAIRDVLEQSIRDGGSSLRDYAQPDGELGYFATRFQVYGR
DGQPCHRDDGGTIRRFAQGGRSTWYCPRCQR
>Mature_270_residues
PELPEVETTVRGLARFLQGERITRTVTNRPDMRFPFPDGLGQALTGATVVSLGRRAKYGLIHTDRDQTMIFHLGMSGRWR
IDPDETDKHDHLLIETADHRFALCDPRRFGWVDLVGTQALDQWPGFAAMGPEPLGDALTIEHLRAALSGRKQAIKLCLLD
QAIVAGLGNIYVCEALWHARIHPRKAGGRVSKQALSLLITAIRDVLEQSIRDGGSSLRDYAQPDGELGYFATRFQVYGRD
GQPCHRDDGGTIRRFAQGGRSTWYCPRCQR

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyr

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger

Homologues:

Organism=Escherichia coli, GI1790066, Length=275, Percent_Identity=41.0909090909091, Blast_Score=184, Evalue=4e-48,
Organism=Escherichia coli, GI1786932, Length=279, Percent_Identity=27.5985663082437, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FPG_ERYLH (Q2N7Y8)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_459000.1
- HSSP:   P42371
- ProteinModelPortal:   Q2N7Y8
- SMR:   Q2N7Y8
- STRING:   Q2N7Y8
- GeneID:   3869870
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_10555
- NMPDR:   fig|314225.3.peg.917
- eggNOG:   COG0266
- HOGENOM:   HBG690070
- OMA:   RSTFYCA
- PhylomeDB:   Q2N7Y8
- ProtClustDB:   PRK01103
- BioCyc:   ELIT314225:ELI_10555-MONOMER
- HAMAP:   MF_00103
- InterPro:   IPR015886
- InterPro:   IPR000191
- InterPro:   IPR012319
- InterPro:   IPR020629
- InterPro:   IPR010979
- InterPro:   IPR000214
- InterPro:   IPR010663
- SMART:   SM00898
- TIGRFAMs:   TIGR00577

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH; PF06827 zf-FPG_IleRS; SSF81624 Form_DNAglyc_cat; SSF46946 Ribosomal_H2TH

EC number: =3.2.2.23; =4.2.99.18

Molecular weight: Translated: 30317; Mature: 30186

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: PS51068 FPG_CAT; PS01242 ZF_FPG_1; PS51066 ZF_FPG_2

Important sites: ACT_SITE 2-2 ACT_SITE 3-3 ACT_SITE 58-58 ACT_SITE 261-261 BINDING 90-90 BINDING 108-108 BINDING 151-151

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELPEVETTVRGLARFLQGERITRTVTNRPDMRFPFPDGLGQALTGATVVSLGRRAKYG
CCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
LIHTDRDQTMIFHLGMSGRWRIDPDETDKHDHLLIETADHRFALCDPRRFGWVDLVGTQA
CEECCCCCEEEEEECCCCEEECCCCCCCCCCEEEEEECCCEEEEECCCCCCEEEEHHHHH
LDQWPGFAAMGPEPLGDALTIEHLRAALSGRKQAIKLCLLDQAIVAGLGNIYVCEALWHA
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
RIHPRKAGGRVSKQALSLLITAIRDVLEQSIRDGGSSLRDYAQPDGELGYFATRFQVYGR
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCEEEEEEEEECC
DGQPCHRDDGGTIRRFAQGGRSTWYCPRCQR
CCCCCCCCCCHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
PELPEVETTVRGLARFLQGERITRTVTNRPDMRFPFPDGLGQALTGATVVSLGRRAKYG
CCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
LIHTDRDQTMIFHLGMSGRWRIDPDETDKHDHLLIETADHRFALCDPRRFGWVDLVGTQA
CEECCCCCEEEEEECCCCEEECCCCCCCCCCEEEEEECCCEEEEECCCCCCEEEEHHHHH
LDQWPGFAAMGPEPLGDALTIEHLRAALSGRKQAIKLCLLDQAIVAGLGNIYVCEALWHA
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
RIHPRKAGGRVSKQALSLLITAIRDVLEQSIRDGGSSLRDYAQPDGELGYFATRFQVYGR
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCEEEEEEEEECC
DGQPCHRDDGGTIRRFAQGGRSTWYCPRCQR
CCCCCCCCCCHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA